Amino acid dipepetide frequency for Actinomadura sp. J1-007

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.83AlaAla: 23.83 ± 0.149	1.168AlaCys: 1.168 ± 0.025	8.511AlaAsp: 8.511 ± 0.066	8.855AlaGlu: 8.855 ± 0.076	4.042AlaPhe: 4.042 ± 0.038	14.586AlaGly: 14.586 ± 0.091	2.68AlaHis: 2.68 ± 0.033	3.866AlaIle: 3.866 ± 0.048	2.587AlaLys: 2.587 ± 0.035	14.748AlaLeu: 14.748 ± 0.105	2.663AlaMet: 2.663 ± 0.033	1.974AlaAsn: 1.974 ± 0.029	7.786AlaPro: 7.786 ± 0.074	3.195AlaGln: 3.195 ± 0.04	12.353AlaArg: 12.353 ± 0.094	6.357AlaSer: 6.357 ± 0.052	6.486AlaThr: 6.486 ± 0.055	12.546AlaVal: 12.546 ± 0.087	2.021AlaTrp: 2.021 ± 0.032	2.761AlaTyr: 2.761 ± 0.031	0.0AlaXaa: 0.0 ± 0.0
Cys	1.12CysAla: 1.12 ± 0.022	0.115CysCys: 0.115 ± 0.009	0.491CysAsp: 0.491 ± 0.013	0.403CysGlu: 0.403 ± 0.012	0.217CysPhe: 0.217 ± 0.008	1.003CysGly: 1.003 ± 0.021	0.196CysHis: 0.196 ± 0.01	0.116CysIle: 0.116 ± 0.007	0.119CysLys: 0.119 ± 0.006	0.734CysLeu: 0.734 ± 0.015	0.14CysMet: 0.14 ± 0.008	0.111CysAsn: 0.111 ± 0.007	0.526CysPro: 0.526 ± 0.016	0.143CysGln: 0.143 ± 0.009	0.666CysArg: 0.666 ± 0.016	0.429CysSer: 0.429 ± 0.016	0.428CysThr: 0.428 ± 0.014	0.674CysVal: 0.674 ± 0.016	0.129CysTrp: 0.129 ± 0.007	0.168CysTyr: 0.168 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.147AspAla: 8.147 ± 0.071	0.37AspCys: 0.37 ± 0.012	3.62AspAsp: 3.62 ± 0.046	3.758AspGlu: 3.758 ± 0.044	1.416AspPhe: 1.416 ± 0.024	6.924AspGly: 6.924 ± 0.061	1.389AspHis: 1.389 ± 0.023	1.567AspIle: 1.567 ± 0.027	0.938AspLys: 0.938 ± 0.02	6.615AspLeu: 6.615 ± 0.056	0.778AspMet: 0.778 ± 0.017	0.786AspAsn: 0.786 ± 0.019	4.731AspPro: 4.731 ± 0.049	1.364AspGln: 1.364 ± 0.023	5.385AspArg: 5.385 ± 0.049	1.964AspSer: 1.964 ± 0.031	2.417AspThr: 2.417 ± 0.028	4.995AspVal: 4.995 ± 0.045	0.888AspTrp: 0.888 ± 0.02	1.071AspTyr: 1.071 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.07GluAla: 7.07 ± 0.062	0.343GluCys: 0.343 ± 0.011	2.654GluAsp: 2.654 ± 0.034	3.121GluGlu: 3.121 ± 0.04	1.376GluPhe: 1.376 ± 0.026	4.096GluGly: 4.096 ± 0.04	1.532GluHis: 1.532 ± 0.022	2.283GluIle: 2.283 ± 0.032	1.084GluLys: 1.084 ± 0.024	6.269GluLeu: 6.269 ± 0.05	0.927GluMet: 0.927 ± 0.019	0.887GluAsn: 0.887 ± 0.019	3.651GluPro: 3.651 ± 0.044	1.733GluGln: 1.733 ± 0.029	6.588GluArg: 6.588 ± 0.069	2.303GluSer: 2.303 ± 0.03	2.734GluThr: 2.734 ± 0.035	4.257GluVal: 4.257 ± 0.048	0.767GluTrp: 0.767 ± 0.019	1.026GluTyr: 1.026 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.856PheAla: 3.856 ± 0.043	0.289PheCys: 0.289 ± 0.011	1.913PheAsp: 1.913 ± 0.03	1.388PheGlu: 1.388 ± 0.023	0.849PhePhe: 0.849 ± 0.021	3.259PheGly: 3.259 ± 0.04	0.591PheHis: 0.591 ± 0.016	0.664PheIle: 0.664 ± 0.018	0.462PheLys: 0.462 ± 0.014	2.591PheLeu: 2.591 ± 0.036	0.423PheMet: 0.423 ± 0.013	0.538PheAsn: 0.538 ± 0.016	1.406PhePro: 1.406 ± 0.024	0.706PheGln: 0.706 ± 0.016	1.926PheArg: 1.926 ± 0.033	1.368PheSer: 1.368 ± 0.025	1.894PheThr: 1.894 ± 0.032	2.321PheVal: 2.321 ± 0.029	0.43PheTrp: 0.43 ± 0.012	0.577PheTyr: 0.577 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	12.657GlyAla: 12.657 ± 0.083	0.869GlyCys: 0.869 ± 0.019	5.642GlyAsp: 5.642 ± 0.047	5.255GlyGlu: 5.255 ± 0.047	2.952GlyPhe: 2.952 ± 0.04	10.222GlyGly: 10.222 ± 0.088	2.283GlyHis: 2.283 ± 0.033	3.186GlyIle: 3.186 ± 0.039	2.214GlyLys: 2.214 ± 0.034	10.014GlyLeu: 10.014 ± 0.065	2.023GlyMet: 2.023 ± 0.028	1.635GlyAsn: 1.635 ± 0.034	6.033GlyPro: 6.033 ± 0.052	2.352GlyGln: 2.352 ± 0.036	9.422GlyArg: 9.422 ± 0.073	5.047GlySer: 5.047 ± 0.052	6.13GlyThr: 6.13 ± 0.061	7.964GlyVal: 7.964 ± 0.061	1.765GlyTrp: 1.765 ± 0.03	2.237GlyTyr: 2.237 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.681HisAla: 2.681 ± 0.037	0.194HisCys: 0.194 ± 0.009	1.307HisAsp: 1.307 ± 0.022	1.085HisGlu: 1.085 ± 0.019	0.597HisPhe: 0.597 ± 0.015	2.31HisGly: 2.31 ± 0.037	0.612HisHis: 0.612 ± 0.017	0.586HisIle: 0.586 ± 0.016	0.272HisLys: 0.272 ± 0.011	2.309HisLeu: 2.309 ± 0.034	0.334HisMet: 0.334 ± 0.011	0.323HisAsn: 0.323 ± 0.01	1.678HisPro: 1.678 ± 0.027	0.529HisGln: 0.529 ± 0.014	2.132HisArg: 2.132 ± 0.031	0.854HisSer: 0.854 ± 0.02	1.009HisThr: 1.009 ± 0.02	1.748HisVal: 1.748 ± 0.029	0.333HisTrp: 0.333 ± 0.01	0.456HisTyr: 0.456 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.866IleAla: 4.866 ± 0.044	0.248IleCys: 0.248 ± 0.011	2.203IleAsp: 2.203 ± 0.03	1.968IleGlu: 1.968 ± 0.029	0.699IlePhe: 0.699 ± 0.018	3.552IleGly: 3.552 ± 0.041	0.508IleHis: 0.508 ± 0.014	0.876IleIle: 0.876 ± 0.018	0.628IleLys: 0.628 ± 0.016	2.326IleLeu: 2.326 ± 0.031	0.475IleMet: 0.475 ± 0.013	0.629IleAsn: 0.629 ± 0.016	1.625IlePro: 1.625 ± 0.025	0.614IleGln: 0.614 ± 0.016	2.146IleArg: 2.146 ± 0.03	1.508IleSer: 1.508 ± 0.028	2.05IleThr: 2.05 ± 0.03	3.008IleVal: 3.008 ± 0.036	0.368IleTrp: 0.368 ± 0.014	0.48IleTyr: 0.48 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.622LysAla: 2.622 ± 0.039	0.102LysCys: 0.102 ± 0.007	1.103LysAsp: 1.103 ± 0.022	0.98LysGlu: 0.98 ± 0.02	0.367LysPhe: 0.367 ± 0.012	1.772LysGly: 1.772 ± 0.033	0.383LysHis: 0.383 ± 0.012	0.81LysIle: 0.81 ± 0.019	0.619LysLys: 0.619 ± 0.023	1.652LysLeu: 1.652 ± 0.029	0.337LysMet: 0.337 ± 0.012	0.43LysAsn: 0.43 ± 0.013	1.291LysPro: 1.291 ± 0.033	0.51LysGln: 0.51 ± 0.017	1.425LysArg: 1.425 ± 0.025	0.956LysSer: 0.956 ± 0.023	1.062LysThr: 1.062 ± 0.024	1.716LysVal: 1.716 ± 0.029	0.233LysTrp: 0.233 ± 0.01	0.361LysTyr: 0.361 ± 0.011	0.0LysXaa: 0.0 ± 0.0
Leu	16.186LeuAla: 16.186 ± 0.119	0.81LeuCys: 0.81 ± 0.018	6.71LeuAsp: 6.71 ± 0.061	4.688LeuGlu: 4.688 ± 0.046	2.552LeuPhe: 2.552 ± 0.036	9.61LeuGly: 9.61 ± 0.062	2.066LeuHis: 2.066 ± 0.029	3.101LeuIle: 3.101 ± 0.036	1.78LeuLys: 1.78 ± 0.027	10.64LeuLeu: 10.64 ± 0.084	1.65LeuMet: 1.65 ± 0.026	1.768LeuAsn: 1.768 ± 0.027	6.444LeuPro: 6.444 ± 0.055	1.909LeuGln: 1.909 ± 0.03	9.028LeuArg: 9.028 ± 0.064	4.983LeuSer: 4.983 ± 0.05	6.008LeuThr: 6.008 ± 0.051	8.763LeuVal: 8.763 ± 0.067	1.291LeuTrp: 1.291 ± 0.026	1.765LeuTyr: 1.765 ± 0.028	0.0LeuXaa: 0.0 ± 0.0
Met	2.477MetAla: 2.477 ± 0.028	0.124MetCys: 0.124 ± 0.007	0.935MetAsp: 0.935 ± 0.02	0.731MetGlu: 0.731 ± 0.018	0.512MetPhe: 0.512 ± 0.013	1.391MetGly: 1.391 ± 0.026	0.321MetHis: 0.321 ± 0.013	0.719MetIle: 0.719 ± 0.017	0.383MetLys: 0.383 ± 0.012	1.812MetLeu: 1.812 ± 0.027	0.309MetMet: 0.309 ± 0.012	0.435MetAsn: 0.435 ± 0.015	1.173MetPro: 1.173 ± 0.019	0.369MetGln: 0.369 ± 0.012	1.631MetArg: 1.631 ± 0.025	1.229MetSer: 1.229 ± 0.022	1.411MetThr: 1.411 ± 0.026	1.373MetVal: 1.373 ± 0.024	0.216MetTrp: 0.216 ± 0.01	0.279MetTyr: 0.279 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.333AsnAla: 2.333 ± 0.032	0.153AsnCys: 0.153 ± 0.008	0.956AsnAsp: 0.956 ± 0.02	0.875AsnGlu: 0.875 ± 0.018	0.406AsnPhe: 0.406 ± 0.013	2.154AsnGly: 2.154 ± 0.036	0.325AsnHis: 0.325 ± 0.011	0.533AsnIle: 0.533 ± 0.014	0.314AsnLys: 0.314 ± 0.013	1.626AsnLeu: 1.626 ± 0.028	0.249AsnMet: 0.249 ± 0.01	0.309AsnAsn: 0.309 ± 0.011	1.218AsnPro: 1.218 ± 0.019	0.407AsnGln: 0.407 ± 0.013	1.175AsnArg: 1.175 ± 0.021	0.662AsnSer: 0.662 ± 0.018	0.913AsnThr: 0.913 ± 0.021	1.528AsnVal: 1.528 ± 0.026	0.238AsnTrp: 0.238 ± 0.01	0.354AsnTyr: 0.354 ± 0.011	0.0AsnXaa: 0.0 ± 0.0
Pro	9.369ProAla: 9.369 ± 0.086	0.405ProCys: 0.405 ± 0.013	4.776ProAsp: 4.776 ± 0.049	4.225ProGlu: 4.225 ± 0.047	1.705ProPhe: 1.705 ± 0.027	7.633ProGly: 7.633 ± 0.062	1.303ProHis: 1.303 ± 0.024	1.526ProIle: 1.526 ± 0.025	1.205ProLys: 1.205 ± 0.027	5.245ProLeu: 5.245 ± 0.05	1.188ProMet: 1.188 ± 0.022	0.99ProAsn: 0.99 ± 0.022	4.694ProPro: 4.694 ± 0.058	1.422ProGln: 1.422 ± 0.027	4.818ProArg: 4.818 ± 0.058	3.665ProSer: 3.665 ± 0.042	2.855ProThr: 2.855 ± 0.036	5.286ProVal: 5.286 ± 0.045	0.943ProTrp: 0.943 ± 0.023	1.391ProTyr: 1.391 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	3.267GlnAla: 3.267 ± 0.041	0.149GlnCys: 0.149 ± 0.007	1.19GlnAsp: 1.19 ± 0.023	1.157GlnGlu: 1.157 ± 0.02	0.573GlnPhe: 0.573 ± 0.015	2.063GlnGly: 2.063 ± 0.034	0.494GlnHis: 0.494 ± 0.013	1.008GlnIle: 1.008 ± 0.023	0.465GlnLys: 0.465 ± 0.016	2.158GlnLeu: 2.158 ± 0.033	0.415GlnMet: 0.415 ± 0.012	0.433GlnAsn: 0.433 ± 0.014	1.398GlnPro: 1.398 ± 0.033	0.876GlnGln: 0.876 ± 0.031	2.307GlnArg: 2.307 ± 0.033	0.998GlnSer: 0.998 ± 0.021	1.123GlnThr: 1.123 ± 0.022	2.163GlnVal: 2.163 ± 0.03	0.402GlnTrp: 0.402 ± 0.014	0.436GlnTyr: 0.436 ± 0.012	0.0GlnXaa: 0.0 ± 0.0
Arg	11.777ArgAla: 11.777 ± 0.085	0.691ArgCys: 0.691 ± 0.018	5.128ArgAsp: 5.128 ± 0.047	5.004ArgGlu: 5.004 ± 0.048	2.537ArgPhe: 2.537 ± 0.032	6.824ArgGly: 6.824 ± 0.064	2.195ArgHis: 2.195 ± 0.03	3.151ArgIle: 3.151 ± 0.035	1.525ArgLys: 1.525 ± 0.027	9.751ArgLeu: 9.751 ± 0.085	1.971ArgMet: 1.971 ± 0.027	1.444ArgAsn: 1.444 ± 0.022	6.29ArgPro: 6.29 ± 0.067	2.074ArgGln: 2.074 ± 0.031	9.75ArgArg: 9.75 ± 0.094	4.489ArgSer: 4.489 ± 0.051	5.384ArgThr: 5.384 ± 0.047	6.69ArgVal: 6.69 ± 0.056	1.526ArgTrp: 1.526 ± 0.021	1.934ArgTyr: 1.934 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.657SerAla: 6.657 ± 0.06	0.404SerCys: 0.404 ± 0.012	2.485SerAsp: 2.485 ± 0.031	2.22SerGlu: 2.22 ± 0.03	1.527SerPhe: 1.527 ± 0.024	6.044SerGly: 6.044 ± 0.053	0.883SerHis: 0.883 ± 0.019	1.359SerIle: 1.359 ± 0.026	0.954SerLys: 0.954 ± 0.024	4.513SerLeu: 4.513 ± 0.042	1.017SerMet: 1.017 ± 0.02	0.784SerAsn: 0.784 ± 0.019	3.356SerPro: 3.356 ± 0.042	0.997SerGln: 0.997 ± 0.022	3.895SerArg: 3.895 ± 0.04	2.677SerSer: 2.677 ± 0.046	2.579SerThr: 2.579 ± 0.038	3.848SerVal: 3.848 ± 0.041	0.852SerTrp: 0.852 ± 0.019	1.128SerTyr: 1.128 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	8.131ThrAla: 8.131 ± 0.066	0.432ThrCys: 0.432 ± 0.015	2.809ThrAsp: 2.809 ± 0.034	2.565ThrGlu: 2.565 ± 0.032	1.609ThrPhe: 1.609 ± 0.027	6.491ThrGly: 6.491 ± 0.055	0.989ThrHis: 0.989 ± 0.024	1.715ThrIle: 1.715 ± 0.031	0.932ThrLys: 0.932 ± 0.02	5.289ThrLeu: 5.289 ± 0.05	1.006ThrMet: 1.006 ± 0.022	0.81ThrAsn: 0.81 ± 0.015	3.726ThrPro: 3.726 ± 0.046	1.017ThrGln: 1.017 ± 0.022	4.111ThrArg: 4.111 ± 0.045	2.843ThrSer: 2.843 ± 0.039	3.112ThrThr: 3.112 ± 0.041	5.408ThrVal: 5.408 ± 0.05	0.869ThrTrp: 0.869 ± 0.018	1.138ThrTyr: 1.138 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	11.681ValAla: 11.681 ± 0.075	0.696ValCys: 0.696 ± 0.016	4.5ValAsp: 4.5 ± 0.049	4.514ValGlu: 4.514 ± 0.044	2.413ValPhe: 2.413 ± 0.033	6.579ValGly: 6.579 ± 0.058	1.856ValHis: 1.856 ± 0.025	2.718ValIle: 2.718 ± 0.034	1.56ValLys: 1.56 ± 0.029	9.638ValLeu: 9.638 ± 0.076	1.379ValMet: 1.379 ± 0.024	1.69ValAsn: 1.69 ± 0.027	5.73ValPro: 5.73 ± 0.055	1.908ValGln: 1.908 ± 0.03	7.933ValArg: 7.933 ± 0.061	4.135ValSer: 4.135 ± 0.04	5.262ValThr: 5.262 ± 0.049	7.818ValVal: 7.818 ± 0.072	1.132ValTrp: 1.132 ± 0.021	1.551ValTyr: 1.551 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.646TrpAla: 1.646 ± 0.03	0.164TrpCys: 0.164 ± 0.009	0.828TrpAsp: 0.828 ± 0.021	0.713TrpGlu: 0.713 ± 0.016	0.486TrpPhe: 0.486 ± 0.013	1.087TrpGly: 1.087 ± 0.023	0.362TrpHis: 0.362 ± 0.011	0.535TrpIle: 0.535 ± 0.015	0.342TrpLys: 0.342 ± 0.012	1.746TrpLeu: 1.746 ± 0.024	0.299TrpMet: 0.299 ± 0.011	0.384TrpAsn: 0.384 ± 0.013	0.874TrpPro: 0.874 ± 0.019	0.445TrpGln: 0.445 ± 0.014	1.626TrpArg: 1.626 ± 0.026	0.855TrpSer: 0.855 ± 0.021	1.103TrpThr: 1.103 ± 0.024	0.942TrpVal: 0.942 ± 0.02	0.346TrpTrp: 0.346 ± 0.012	0.332TrpTyr: 0.332 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.641TyrAla: 2.641 ± 0.03	0.201TyrCys: 0.201 ± 0.008	1.361TyrAsp: 1.361 ± 0.03	1.198TyrGlu: 1.198 ± 0.022	0.615TyrPhe: 0.615 ± 0.019	2.303TyrGly: 2.303 ± 0.032	0.383TyrHis: 0.383 ± 0.012	0.457TyrIle: 0.457 ± 0.014	0.346TyrLys: 0.346 ± 0.014	2.075TyrLeu: 2.075 ± 0.031	0.246TyrMet: 0.246 ± 0.01	0.366TyrAsn: 0.366 ± 0.013	1.015TyrPro: 1.015 ± 0.02	0.506TyrGln: 0.506 ± 0.016	1.82TyrArg: 1.82 ± 0.03	0.854TyrSer: 0.854 ± 0.021	1.045TyrThr: 1.045 ± 0.022	1.643TyrVal: 1.643 ± 0.028	0.366TyrTrp: 0.366 ± 0.012	0.422TyrTyr: 0.422 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8101 proteins (2547833 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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