Escherichia phage aldrigsur

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Bonnellvirus; unclassified Bonnellvirus

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9WVS8|A0A6B9WVS8_9CAUD Uncharacterized protein OS=Escherichia phage aldrigsur OX=2696378 GN=aldrigsur_10 PE=4 SV=1
MM1 pKa = 7.47ALVDD5 pKa = 3.57STAAEE10 pKa = 3.86RR11 pKa = 11.84AEE13 pKa = 5.0FVAACNMAAKK23 pKa = 9.82YY24 pKa = 10.73LNVWTEE30 pKa = 4.4DD31 pKa = 3.66YY32 pKa = 11.2FFDD35 pKa = 3.72TTNTEE40 pKa = 3.74LVAYY44 pKa = 7.89YY45 pKa = 8.17QTFVTRR51 pKa = 11.84VQTALTNLTNGGAA64 pKa = 3.51

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9WS49|A0A6B9WS49_9CAUD Uncharacterized protein OS=Escherichia phage aldrigsur OX=2696378 GN=aldrigsur_33 PE=4 SV=1
MM1 pKa = 7.8EE2 pKa = 4.56EE3 pKa = 3.64RR4 pKa = 11.84TYY6 pKa = 10.97RR7 pKa = 11.84AAPLNQRR14 pKa = 11.84EE15 pKa = 4.14QATIRR20 pKa = 11.84AQYY23 pKa = 10.49RR24 pKa = 11.84KK25 pKa = 10.09GDD27 pKa = 3.59AFRR30 pKa = 11.84GAEE33 pKa = 3.82ALAEE37 pKa = 4.11RR38 pKa = 11.84FNVPPADD45 pKa = 3.33VVAVVNFTYY54 pKa = 10.52RR55 pKa = 11.84KK56 pKa = 9.82GKK58 pKa = 9.52QKK60 pKa = 10.4HH61 pKa = 6.33DD62 pKa = 3.52KK63 pKa = 10.67

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12665

56

1036

234.5

25.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.225 ± 0.592

0.94 ± 0.155

6.103 ± 0.3

6.182 ± 0.391

3.466 ± 0.201

7.833 ± 0.367

2.037 ± 0.229

4.659 ± 0.242

4.358 ± 0.303

8.133 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.866 ± 0.303

4.145 ± 0.316

4.343 ± 0.299

4.714 ± 0.45

5.638 ± 0.317

5.653 ± 0.316

6.151 ± 0.487

7.469 ± 0.385

1.516 ± 0.155

3.569 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski