Amino acid dipepetide frequency for Escherichia phage aldrigsur

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.266AlaAla: 13.266 ± 1.844
1.105AlaCys: 1.105 ± 0.318
5.37AlaAsp: 5.37 ± 0.641
7.107AlaGlu: 7.107 ± 0.835
3.711AlaPhe: 3.711 ± 0.527
7.423AlaGly: 7.423 ± 0.969
1.974AlaHis: 1.974 ± 0.369
4.58AlaIle: 4.58 ± 0.519
4.185AlaLys: 4.185 ± 0.603
9.397AlaLeu: 9.397 ± 1.182
3.159AlaMet: 3.159 ± 0.652
3.711AlaAsn: 3.711 ± 0.654
3.948AlaPro: 3.948 ± 0.733
5.212AlaGln: 5.212 ± 0.895
5.054AlaArg: 5.054 ± 0.874
5.291AlaSer: 5.291 ± 0.76
5.606AlaThr: 5.606 ± 1.058
7.423AlaVal: 7.423 ± 0.809
1.184AlaTrp: 1.184 ± 0.281
3.553AlaTyr: 3.553 ± 0.536
0.0AlaXaa: 0.0 ± 0.0
Cys
0.79CysAla: 0.79 ± 0.297
0.079CysCys: 0.079 ± 0.064
0.395CysAsp: 0.395 ± 0.2
0.316CysGlu: 0.316 ± 0.138
0.237CysPhe: 0.237 ± 0.114
0.711CysGly: 0.711 ± 0.329
0.316CysHis: 0.316 ± 0.228
0.553CysIle: 0.553 ± 0.218
0.632CysLys: 0.632 ± 0.244
0.948CysLeu: 0.948 ± 0.242
0.316CysMet: 0.316 ± 0.172
0.395CysAsn: 0.395 ± 0.18
0.632CysPro: 0.632 ± 0.266
0.158CysGln: 0.158 ± 0.117
0.632CysArg: 0.632 ± 0.224
0.79CysSer: 0.79 ± 0.257
0.158CysThr: 0.158 ± 0.096
0.869CysVal: 0.869 ± 0.257
0.158CysTrp: 0.158 ± 0.11
0.316CysTyr: 0.316 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
6.238AspAla: 6.238 ± 0.706
0.395AspCys: 0.395 ± 0.207
5.37AspAsp: 5.37 ± 0.796
4.738AspGlu: 4.738 ± 0.659
2.132AspPhe: 2.132 ± 0.441
5.291AspGly: 5.291 ± 0.708
0.632AspHis: 0.632 ± 0.24
4.185AspIle: 4.185 ± 0.484
2.922AspLys: 2.922 ± 0.421
4.027AspLeu: 4.027 ± 0.541
1.579AspMet: 1.579 ± 0.414
2.29AspAsn: 2.29 ± 0.376
3.08AspPro: 3.08 ± 0.605
1.027AspGln: 1.027 ± 0.283
2.922AspArg: 2.922 ± 0.381
3.869AspSer: 3.869 ± 0.693
3.79AspThr: 3.79 ± 0.554
3.001AspVal: 3.001 ± 0.523
1.184AspTrp: 1.184 ± 0.343
2.369AspTyr: 2.369 ± 0.455
0.0AspXaa: 0.0 ± 0.0
Glu
5.843GluAla: 5.843 ± 0.844
0.711GluCys: 0.711 ± 0.275
3.395GluAsp: 3.395 ± 0.602
3.869GluGlu: 3.869 ± 0.632
2.764GluPhe: 2.764 ± 0.474
4.343GluGly: 4.343 ± 0.489
1.263GluHis: 1.263 ± 0.313
1.737GluIle: 1.737 ± 0.402
2.448GluLys: 2.448 ± 0.501
5.449GluLeu: 5.449 ± 0.751
2.053GluMet: 2.053 ± 0.472
2.448GluAsn: 2.448 ± 0.436
2.211GluPro: 2.211 ± 0.539
3.474GluGln: 3.474 ± 0.572
3.553GluArg: 3.553 ± 0.526
3.001GluSer: 3.001 ± 0.471
4.106GluThr: 4.106 ± 0.726
5.291GluVal: 5.291 ± 0.926
1.421GluTrp: 1.421 ± 0.323
2.448GluTyr: 2.448 ± 0.579
0.0GluXaa: 0.0 ± 0.0
Phe
2.843PheAla: 2.843 ± 0.487
0.395PheCys: 0.395 ± 0.164
2.843PheAsp: 2.843 ± 0.427
2.132PheGlu: 2.132 ± 0.468
0.948PhePhe: 0.948 ± 0.196
2.922PheGly: 2.922 ± 0.458
0.395PheHis: 0.395 ± 0.174
2.448PheIle: 2.448 ± 0.404
1.974PheLys: 1.974 ± 0.462
2.132PheLeu: 2.132 ± 0.383
1.027PheMet: 1.027 ± 0.275
1.816PheAsn: 1.816 ± 0.416
1.895PhePro: 1.895 ± 0.346
1.105PheGln: 1.105 ± 0.32
1.737PheArg: 1.737 ± 0.394
1.579PheSer: 1.579 ± 0.375
2.369PheThr: 2.369 ± 0.384
2.922PheVal: 2.922 ± 0.444
0.632PheTrp: 0.632 ± 0.214
0.553PheTyr: 0.553 ± 0.181
0.0PheXaa: 0.0 ± 0.0
Gly
7.423GlyAla: 7.423 ± 1.256
0.869GlyCys: 0.869 ± 0.226
4.264GlyAsp: 4.264 ± 0.595
5.054GlyGlu: 5.054 ± 0.736
1.816GlyPhe: 1.816 ± 0.445
6.396GlyGly: 6.396 ± 1.021
1.421GlyHis: 1.421 ± 0.392
4.659GlyIle: 4.659 ± 0.598
4.185GlyLys: 4.185 ± 0.83
5.449GlyLeu: 5.449 ± 0.672
2.448GlyMet: 2.448 ± 0.372
3.238GlyAsn: 3.238 ± 0.512
1.658GlyPro: 1.658 ± 0.226
3.79GlyGln: 3.79 ± 0.634
5.054GlyArg: 5.054 ± 0.515
4.896GlySer: 4.896 ± 0.735
6.001GlyThr: 6.001 ± 1.054
5.291GlyVal: 5.291 ± 0.76
1.263GlyTrp: 1.263 ± 0.291
3.159GlyTyr: 3.159 ± 0.381
0.0GlyXaa: 0.0 ± 0.0
His
1.658HisAla: 1.658 ± 0.363
0.316HisCys: 0.316 ± 0.129
1.5HisAsp: 1.5 ± 0.376
1.342HisGlu: 1.342 ± 0.306
0.79HisPhe: 0.79 ± 0.207
1.658HisGly: 1.658 ± 0.31
0.869HisHis: 0.869 ± 0.307
0.474HisIle: 0.474 ± 0.199
1.105HisLys: 1.105 ± 0.327
2.132HisLeu: 2.132 ± 0.418
0.632HisMet: 0.632 ± 0.186
0.553HisAsn: 0.553 ± 0.156
0.79HisPro: 0.79 ± 0.311
0.395HisGln: 0.395 ± 0.159
0.869HisArg: 0.869 ± 0.264
1.027HisSer: 1.027 ± 0.284
1.184HisThr: 1.184 ± 0.288
1.737HisVal: 1.737 ± 0.497
0.395HisTrp: 0.395 ± 0.205
0.948HisTyr: 0.948 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
4.185IleAla: 4.185 ± 0.506
0.553IleCys: 0.553 ± 0.316
3.553IleAsp: 3.553 ± 0.478
2.922IleGlu: 2.922 ± 0.42
1.105IlePhe: 1.105 ± 0.264
4.106IleGly: 4.106 ± 0.574
0.948IleHis: 0.948 ± 0.208
2.843IleIle: 2.843 ± 0.516
1.658IleLys: 1.658 ± 0.387
2.843IleLeu: 2.843 ± 0.479
1.263IleMet: 1.263 ± 0.268
2.132IleAsn: 2.132 ± 0.445
2.685IlePro: 2.685 ± 0.389
2.369IleGln: 2.369 ± 0.308
3.238IleArg: 3.238 ± 0.442
1.974IleSer: 1.974 ± 0.431
2.448IleThr: 2.448 ± 0.527
4.027IleVal: 4.027 ± 0.589
0.395IleTrp: 0.395 ± 0.191
1.342IleTyr: 1.342 ± 0.279
0.0IleXaa: 0.0 ± 0.0
Lys
5.527LysAla: 5.527 ± 0.874
0.474LysCys: 0.474 ± 0.186
2.606LysAsp: 2.606 ± 0.717
2.922LysGlu: 2.922 ± 0.463
1.816LysPhe: 1.816 ± 0.378
2.369LysGly: 2.369 ± 0.497
1.184LysHis: 1.184 ± 0.38
0.553LysIle: 0.553 ± 0.238
1.816LysLys: 1.816 ± 0.438
4.738LysLeu: 4.738 ± 0.843
0.948LysMet: 0.948 ± 0.245
0.948LysAsn: 0.948 ± 0.264
2.29LysPro: 2.29 ± 0.345
2.764LysGln: 2.764 ± 0.442
2.211LysArg: 2.211 ± 0.414
2.685LysSer: 2.685 ± 0.377
2.527LysThr: 2.527 ± 0.524
3.316LysVal: 3.316 ± 0.579
0.79LysTrp: 0.79 ± 0.274
1.105LysTyr: 1.105 ± 0.257
0.0LysXaa: 0.0 ± 0.0
Leu
8.844LeuAla: 8.844 ± 0.85
0.474LeuCys: 0.474 ± 0.189
5.133LeuAsp: 5.133 ± 0.581
5.291LeuGlu: 5.291 ± 0.545
3.395LeuPhe: 3.395 ± 0.573
5.922LeuGly: 5.922 ± 0.743
2.527LeuHis: 2.527 ± 0.449
2.527LeuIle: 2.527 ± 0.388
2.764LeuLys: 2.764 ± 0.424
6.791LeuLeu: 6.791 ± 0.782
2.053LeuMet: 2.053 ± 0.416
3.711LeuAsn: 3.711 ± 0.514
4.027LeuPro: 4.027 ± 0.543
4.027LeuGln: 4.027 ± 0.445
4.185LeuArg: 4.185 ± 0.625
4.817LeuSer: 4.817 ± 0.692
5.449LeuThr: 5.449 ± 0.871
5.37LeuVal: 5.37 ± 0.539
1.421LeuTrp: 1.421 ± 0.37
2.527LeuTyr: 2.527 ± 0.446
0.0LeuXaa: 0.0 ± 0.0
Met
3.08MetAla: 3.08 ± 0.594
0.237MetCys: 0.237 ± 0.127
1.263MetAsp: 1.263 ± 0.368
0.632MetGlu: 0.632 ± 0.211
0.632MetPhe: 0.632 ± 0.214
2.132MetGly: 2.132 ± 0.441
0.711MetHis: 0.711 ± 0.209
0.711MetIle: 0.711 ± 0.312
1.342MetLys: 1.342 ± 0.345
2.843MetLeu: 2.843 ± 0.552
1.579MetMet: 1.579 ± 0.55
1.421MetAsn: 1.421 ± 0.319
1.184MetPro: 1.184 ± 0.263
2.053MetGln: 2.053 ± 0.535
1.658MetArg: 1.658 ± 0.517
1.5MetSer: 1.5 ± 0.303
2.369MetThr: 2.369 ± 0.383
1.816MetVal: 1.816 ± 0.38
0.553MetTrp: 0.553 ± 0.278
0.948MetTyr: 0.948 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
3.553AsnAla: 3.553 ± 0.603
0.395AsnCys: 0.395 ± 0.215
1.737AsnAsp: 1.737 ± 0.298
2.369AsnGlu: 2.369 ± 0.331
1.5AsnPhe: 1.5 ± 0.399
3.316AsnGly: 3.316 ± 0.633
0.869AsnHis: 0.869 ± 0.306
3.001AsnIle: 3.001 ± 0.461
2.606AsnLys: 2.606 ± 0.419
3.238AsnLeu: 3.238 ± 0.462
1.105AsnMet: 1.105 ± 0.259
1.737AsnAsn: 1.737 ± 0.355
2.29AsnPro: 2.29 ± 0.56
1.658AsnGln: 1.658 ± 0.324
1.974AsnArg: 1.974 ± 0.311
2.527AsnSer: 2.527 ± 0.538
2.448AsnThr: 2.448 ± 0.49
4.264AsnVal: 4.264 ± 0.751
0.474AsnTrp: 0.474 ± 0.2
0.395AsnTyr: 0.395 ± 0.163
0.0AsnXaa: 0.0 ± 0.0
Pro
4.106ProAla: 4.106 ± 0.49
0.553ProCys: 0.553 ± 0.211
3.395ProAsp: 3.395 ± 0.428
3.711ProGlu: 3.711 ± 0.569
1.184ProPhe: 1.184 ± 0.312
3.159ProGly: 3.159 ± 0.48
0.948ProHis: 0.948 ± 0.335
1.5ProIle: 1.5 ± 0.275
1.658ProLys: 1.658 ± 0.484
2.606ProLeu: 2.606 ± 0.501
0.711ProMet: 0.711 ± 0.213
2.448ProAsn: 2.448 ± 0.562
1.816ProPro: 1.816 ± 0.432
1.895ProGln: 1.895 ± 0.768
2.132ProArg: 2.132 ± 0.371
2.685ProSer: 2.685 ± 0.437
2.764ProThr: 2.764 ± 0.446
3.316ProVal: 3.316 ± 0.527
0.632ProTrp: 0.632 ± 0.209
2.211ProTyr: 2.211 ± 0.496
0.0ProXaa: 0.0 ± 0.0
Gln
5.527GlnAla: 5.527 ± 0.8
0.079GlnCys: 0.079 ± 0.073
2.369GlnAsp: 2.369 ± 0.333
2.132GlnGlu: 2.132 ± 0.397
2.448GlnPhe: 2.448 ± 0.517
3.159GlnGly: 3.159 ± 0.584
0.948GlnHis: 0.948 ± 0.25
1.816GlnIle: 1.816 ± 0.477
1.421GlnLys: 1.421 ± 0.381
3.474GlnLeu: 3.474 ± 0.653
1.579GlnMet: 1.579 ± 0.312
1.816GlnAsn: 1.816 ± 0.609
2.211GlnPro: 2.211 ± 0.957
3.711GlnGln: 3.711 ± 1.255
3.869GlnArg: 3.869 ± 0.609
2.053GlnSer: 2.053 ± 0.424
2.606GlnThr: 2.606 ± 0.544
3.474GlnVal: 3.474 ± 0.554
0.632GlnTrp: 0.632 ± 0.187
1.816GlnTyr: 1.816 ± 0.309
0.0GlnXaa: 0.0 ± 0.0
Arg
5.527ArgAla: 5.527 ± 0.945
0.474ArgCys: 0.474 ± 0.192
3.474ArgAsp: 3.474 ± 0.681
3.79ArgGlu: 3.79 ± 0.606
2.132ArgPhe: 2.132 ± 0.335
4.343ArgGly: 4.343 ± 0.533
1.027ArgHis: 1.027 ± 0.286
3.159ArgIle: 3.159 ± 0.407
2.606ArgLys: 2.606 ± 0.425
5.291ArgLeu: 5.291 ± 0.473
1.737ArgMet: 1.737 ± 0.358
1.737ArgAsn: 1.737 ± 0.41
1.658ArgPro: 1.658 ± 0.549
2.448ArgGln: 2.448 ± 0.33
4.027ArgArg: 4.027 ± 0.822
2.448ArgSer: 2.448 ± 0.431
3.08ArgThr: 3.08 ± 0.398
4.027ArgVal: 4.027 ± 0.659
1.027ArgTrp: 1.027 ± 0.287
2.369ArgTyr: 2.369 ± 0.433
0.0ArgXaa: 0.0 ± 0.0
Ser
5.606SerAla: 5.606 ± 0.506
0.079SerCys: 0.079 ± 0.101
3.001SerAsp: 3.001 ± 0.456
2.527SerGlu: 2.527 ± 0.508
2.369SerPhe: 2.369 ± 0.382
5.764SerGly: 5.764 ± 0.507
0.948SerHis: 0.948 ± 0.299
3.159SerIle: 3.159 ± 0.422
2.685SerLys: 2.685 ± 0.404
4.422SerLeu: 4.422 ± 0.555
1.5SerMet: 1.5 ± 0.364
2.448SerAsn: 2.448 ± 0.426
3.08SerPro: 3.08 ± 0.515
2.132SerGln: 2.132 ± 0.416
2.606SerArg: 2.606 ± 0.474
3.08SerSer: 3.08 ± 0.734
3.948SerThr: 3.948 ± 0.867
4.106SerVal: 4.106 ± 0.705
0.79SerTrp: 0.79 ± 0.242
2.29SerTyr: 2.29 ± 0.44
0.0SerXaa: 0.0 ± 0.0
Thr
7.581ThrAla: 7.581 ± 0.944
0.474ThrCys: 0.474 ± 0.173
2.922ThrAsp: 2.922 ± 0.473
2.606ThrGlu: 2.606 ± 0.477
1.895ThrPhe: 1.895 ± 0.443
5.449ThrGly: 5.449 ± 0.599
0.869ThrHis: 0.869 ± 0.217
2.843ThrIle: 2.843 ± 0.449
2.843ThrLys: 2.843 ± 0.466
5.37ThrLeu: 5.37 ± 0.602
1.342ThrMet: 1.342 ± 0.396
3.474ThrAsn: 3.474 ± 0.845
2.29ThrPro: 2.29 ± 0.447
2.369ThrGln: 2.369 ± 0.549
3.001ThrArg: 3.001 ± 0.414
4.501ThrSer: 4.501 ± 0.725
3.159ThrThr: 3.159 ± 0.725
5.212ThrVal: 5.212 ± 1.073
0.632ThrTrp: 0.632 ± 0.201
2.685ThrTyr: 2.685 ± 0.461
0.0ThrXaa: 0.0 ± 0.0
Val
6.87ValAla: 6.87 ± 0.853
0.869ValCys: 0.869 ± 0.283
4.975ValAsp: 4.975 ± 0.691
5.212ValGlu: 5.212 ± 0.708
2.053ValPhe: 2.053 ± 0.504
5.449ValGly: 5.449 ± 0.685
1.421ValHis: 1.421 ± 0.328
3.79ValIle: 3.79 ± 0.472
3.553ValLys: 3.553 ± 0.579
5.054ValLeu: 5.054 ± 0.602
1.737ValMet: 1.737 ± 0.451
3.08ValAsn: 3.08 ± 0.573
3.632ValPro: 3.632 ± 0.592
3.553ValGln: 3.553 ± 0.681
4.58ValArg: 4.58 ± 0.862
4.501ValSer: 4.501 ± 0.536
4.817ValThr: 4.817 ± 0.848
5.843ValVal: 5.843 ± 0.709
1.027ValTrp: 1.027 ± 0.276
2.685ValTyr: 2.685 ± 0.401
0.0ValXaa: 0.0 ± 0.0
Trp
0.948TrpAla: 0.948 ± 0.286
0.474TrpCys: 0.474 ± 0.177
1.421TrpAsp: 1.421 ± 0.228
0.711TrpGlu: 0.711 ± 0.206
1.027TrpPhe: 1.027 ± 0.302
1.027TrpGly: 1.027 ± 0.266
0.316TrpHis: 0.316 ± 0.127
0.474TrpIle: 0.474 ± 0.205
0.158TrpLys: 0.158 ± 0.107
2.29TrpLeu: 2.29 ± 0.474
0.474TrpMet: 0.474 ± 0.208
0.395TrpAsn: 0.395 ± 0.162
0.553TrpPro: 0.553 ± 0.218
0.79TrpGln: 0.79 ± 0.172
0.632TrpArg: 0.632 ± 0.198
1.105TrpSer: 1.105 ± 0.236
0.474TrpThr: 0.474 ± 0.175
0.869TrpVal: 0.869 ± 0.276
0.474TrpTrp: 0.474 ± 0.229
0.553TrpTyr: 0.553 ± 0.247
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.843TyrAla: 2.843 ± 0.553
0.395TyrCys: 0.395 ± 0.179
2.053TyrAsp: 2.053 ± 0.353
2.448TyrGlu: 2.448 ± 0.479
0.711TyrPhe: 0.711 ± 0.207
3.395TyrGly: 3.395 ± 0.426
0.79TyrHis: 0.79 ± 0.258
1.579TyrIle: 1.579 ± 0.449
1.027TyrLys: 1.027 ± 0.238
2.843TyrLeu: 2.843 ± 0.532
1.342TyrMet: 1.342 ± 0.306
1.658TyrAsn: 1.658 ± 0.249
1.5TyrPro: 1.5 ± 0.272
2.211TyrGln: 2.211 ± 0.382
2.448TyrArg: 2.448 ± 0.522
2.211TyrSer: 2.211 ± 0.443
2.211TyrThr: 2.211 ± 0.566
2.527TyrVal: 2.527 ± 0.505
0.079TyrTrp: 0.079 ± 0.064
1.421TyrTyr: 1.421 ± 0.297
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (12665 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski