Gryllus bimaculatus nudivirus
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4L1W7|A4L1W7_9VIRU Uncharacterized protein OS=Gryllus bimaculatus nudivirus OX=432587 PE=4 SV=1
MM1 pKa = 7.29 MNYY4 pKa = 10.21 LGLILLVVISIICLVLCILLSNIKK28 pKa = 10.76 NNINLDD34 pKa = 3.63 KK35 pKa = 11.16 QEE37 pKa = 4.16 NRR39 pKa = 11.84 LDD41 pKa = 3.88 LDD43 pKa = 3.6 GFQNIITKK51 pKa = 9.78 RR52 pKa = 11.84 DD53 pKa = 3.59 CSSEE57 pKa = 4.07 TVYY60 pKa = 10.95 SINDD64 pKa = 3.53 AQCNQACYY72 pKa = 10.66 GNVGQYY78 pKa = 10.26 VSQNGICINSLILQSSVIEE97 pKa = 4.33 KK98 pKa = 10.57 EE99 pKa = 3.92 CDD101 pKa = 3.23 PKK103 pKa = 11.07 KK104 pKa = 10.99 GVIAYY109 pKa = 9.5 LIGNTQTGQTSLKK122 pKa = 9.78 CLSVDD127 pKa = 5.57 LGIQSNDD134 pKa = 3.05 PDD136 pKa = 4.46 GPNVICQNGSIDD148 pKa = 3.57 IDD150 pKa = 3.86 YY151 pKa = 10.9 LKK153 pKa = 10.51 EE154 pKa = 3.87 FPQIEE159 pKa = 4.38 NCKK162 pKa = 10.19 CMPSDD167 pKa = 3.77 VVGLIPYY174 pKa = 6.75 TTSIRR179 pKa = 11.84 QHH181 pKa = 6.46 ASCVSLNLYY190 pKa = 9.87 DD191 pKa = 3.75 ALKK194 pKa = 10.99 YY195 pKa = 10.63 NDD197 pKa = 5.19 LIII200 pKa = 5.19
Molecular weight: 22.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.409
IPC2_protein 4.495
IPC_protein 4.418
Toseland 4.215
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.342
Rodwell 4.253
Grimsley 4.126
Solomon 4.406
Lehninger 4.355
Nozaki 4.52
DTASelect 4.762
Thurlkill 4.266
EMBOSS 4.342
Sillero 4.546
Patrickios 0.337
IPC_peptide 4.393
IPC2_peptide 4.52
IPC2.peptide.svr19 4.453
Protein with the highest isoelectric point:
>tr|A4L237|A4L237_9VIRU Putative guanylate kinase OS=Gryllus bimaculatus nudivirus OX=432587 GN=gk PE=4 SV=1
MM1 pKa = 6.77 MQLEE5 pKa = 4.33 SRR7 pKa = 11.84 GNSRR11 pKa = 11.84 SRR13 pKa = 11.84 SRR15 pKa = 11.84 SRR17 pKa = 11.84 SRR19 pKa = 11.84 SKK21 pKa = 10.68 SRR23 pKa = 11.84 SRR25 pKa = 11.84 SNSRR29 pKa = 11.84 SRR31 pKa = 11.84 SRR33 pKa = 11.84 SKK35 pKa = 10.68 SRR37 pKa = 11.84 SRR39 pKa = 11.84 SNSRR43 pKa = 11.84 SRR45 pKa = 11.84 RR46 pKa = 11.84 SKK48 pKa = 9.25 RR49 pKa = 11.84 RR50 pKa = 11.84 SRR52 pKa = 11.84 SRR54 pKa = 11.84 SRR56 pKa = 11.84 CKK58 pKa = 9.61 PSSRR62 pKa = 11.84 SRR64 pKa = 11.84 SRR66 pKa = 11.84 SGRR69 pKa = 11.84 RR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 SRR74 pKa = 11.84 SRR76 pKa = 11.84 SRR78 pKa = 11.84 SRR80 pKa = 11.84 SRR82 pKa = 11.84 SCSRR86 pKa = 3.42
Molecular weight: 10.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.496
IPC2_protein 11.111
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.349
Rodwell 12.427
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.135
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.162
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
98
0
98
30144
56
1329
307.6
35.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.896 ± 0.129
2.292 ± 0.139
5.477 ± 0.128
6.326 ± 0.254
5.66 ± 0.225
2.737 ± 0.161
1.755 ± 0.117
9.428 ± 0.211
9.375 ± 0.228
9.302 ± 0.214
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.738 ± 0.075
9.073 ± 0.206
2.999 ± 0.124
3.032 ± 0.172
3.112 ± 0.156
7.613 ± 0.227
5.951 ± 0.166
5.258 ± 0.158
0.62 ± 0.049
5.354 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here