Gordonia phage Fosterous
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3M9R2|A0A3G3M9R2_9CAUD Uncharacterized protein OS=Gordonia phage Fosterous OX=2483668 GN=47 PE=4 SV=1
MM1 pKa = 7.52 SWTEE5 pKa = 3.81 DD6 pKa = 3.54 CNACGHH12 pKa = 5.69 THH14 pKa = 7.4 PDD16 pKa = 3.65 EE17 pKa = 4.91 CDD19 pKa = 3.81 CPCGCTMHH27 pKa = 7.33 PVDD30 pKa = 3.83 TWDD33 pKa = 3.45 MTEE36 pKa = 4.44 LPGDD40 pKa = 3.66 EE41 pKa = 4.19
Molecular weight: 4.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.901
IPC2_protein 3.834
IPC_protein 3.706
Toseland 3.516
ProMoST 3.846
Dawson 3.719
Bjellqvist 3.986
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.846
Patrickios 0.006
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.864
Protein with the highest isoelectric point:
>tr|A0A3G3M9P3|A0A3G3M9P3_9CAUD Uncharacterized protein OS=Gordonia phage Fosterous OX=2483668 GN=27 PE=4 SV=1
MM1 pKa = 7.09 TADD4 pKa = 3.68 PAIAAAQRR12 pKa = 11.84 AMSNPEE18 pKa = 4.02 VEE20 pKa = 4.61 AAWIDD25 pKa = 3.58 GGNDD29 pKa = 3.24 LAIAAARR36 pKa = 11.84 EE37 pKa = 3.88 ALAPLRR43 pKa = 11.84 KK44 pKa = 9.32 LHH46 pKa = 6.8 RR47 pKa = 11.84 PTRR50 pKa = 11.84 SNEE53 pKa = 3.55 WQHH56 pKa = 6.76 AKK58 pKa = 9.2 TKK60 pKa = 10.59 CPAGHH65 pKa = 7.54 DD66 pKa = 3.81 YY67 pKa = 11.49 AGDD70 pKa = 3.6 NLIVVQRR77 pKa = 11.84 PNRR80 pKa = 11.84 AGPEE84 pKa = 4.0 RR85 pKa = 11.84 RR86 pKa = 11.84 CRR88 pKa = 11.84 TCTRR92 pKa = 11.84 ATEE95 pKa = 4.0 RR96 pKa = 11.84 RR97 pKa = 11.84 SRR99 pKa = 11.84 ARR101 pKa = 11.84 KK102 pKa = 7.61 TGVV105 pKa = 2.67
Molecular weight: 11.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.37
IPC_protein 10.101
Toseland 10.657
ProMoST 10.365
Dawson 10.716
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.804
Grimsley 10.745
Solomon 10.891
Lehninger 10.862
Nozaki 10.672
DTASelect 10.423
Thurlkill 10.643
EMBOSS 11.067
Sillero 10.657
Patrickios 10.599
IPC_peptide 10.891
IPC2_peptide 9.78
IPC2.peptide.svr19 8.606
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
87
0
87
18839
37
1607
216.5
23.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.002 ± 0.406
0.897 ± 0.146
7.495 ± 0.457
5.664 ± 0.379
2.458 ± 0.16
8.626 ± 0.399
2.203 ± 0.191
4.523 ± 0.255
2.505 ± 0.195
7.368 ± 0.26
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.099
2.606 ± 0.149
6.28 ± 0.258
3.79 ± 0.181
7.644 ± 0.431
4.761 ± 0.242
6.8 ± 0.283
7.819 ± 0.274
2.144 ± 0.13
2.198 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here