Mucilaginibacter corticis
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4741 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A556MX23|A0A556MX23_9SPHI Uncharacterized protein OS=Mucilaginibacter corticis OX=2597670 GN=FO440_09915 PE=4 SV=1
MM1 pKa = 7.34 MNPVQTSIPQADD13 pKa = 3.65 GSDD16 pKa = 3.2 RR17 pKa = 11.84 QVIIEE22 pKa = 4.87 PILEE26 pKa = 4.25 KK27 pKa = 10.13 HH28 pKa = 5.63 DD29 pKa = 4.1 HH30 pKa = 6.65 GYY32 pKa = 10.91 SSTGVYY38 pKa = 9.99 IIYY41 pKa = 10.31 KK42 pKa = 10.15 DD43 pKa = 3.43 AFSDD47 pKa = 3.64 EE48 pKa = 4.31 THH50 pKa = 7.06 LFTEE54 pKa = 5.13 PPEE57 pKa = 4.65 SDD59 pKa = 3.93 GPADD63 pKa = 5.33 DD64 pKa = 6.36 LPDD67 pKa = 3.96 EE68 pKa = 4.36 QNPDD72 pKa = 3.55 YY73 pKa = 10.93 LGKK76 pKa = 10.37 FVFDD80 pKa = 4.88 DD81 pKa = 4.25 GSLNHH86 pKa = 6.5 FEE88 pKa = 4.32 GQVLSHH94 pKa = 7.11 AEE96 pKa = 3.75 QAYY99 pKa = 9.71 LAVFIEE105 pKa = 4.27 NWQEE109 pKa = 3.52 PDD111 pKa = 3.07 VV112 pKa = 4.03
Molecular weight: 12.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.935
IPC_protein 3.897
Toseland 3.694
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.732
Grimsley 3.605
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.215
Thurlkill 3.745
EMBOSS 3.821
Sillero 4.012
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.916
Protein with the highest isoelectric point:
>tr|A0A556MI93|A0A556MI93_9SPHI Sigma-70 family RNA polymerase sigma factor OS=Mucilaginibacter corticis OX=2597670 GN=FO440_17540 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.54 RR3 pKa = 11.84 TFQPSQRR10 pKa = 11.84 KK11 pKa = 8.72 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.3 HH16 pKa = 3.93 GFRR19 pKa = 11.84 EE20 pKa = 4.18 RR21 pKa = 11.84 MSTANGRR28 pKa = 11.84 RR29 pKa = 11.84 ILAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.02 GRR39 pKa = 11.84 KK40 pKa = 8.51 KK41 pKa = 9.86 LTVSSEE47 pKa = 4.16 SRR49 pKa = 11.84 HH50 pKa = 5.16 KK51 pKa = 10.96 AA52 pKa = 3.08
Molecular weight: 6.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 10.745
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.223
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.959
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4741
0
4741
1574943
25
2344
332.2
37.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.805 ± 0.035
0.793 ± 0.011
5.677 ± 0.027
5.207 ± 0.034
4.839 ± 0.025
6.838 ± 0.035
1.945 ± 0.015
7.301 ± 0.033
7.042 ± 0.03
9.451 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.238 ± 0.016
5.563 ± 0.029
4.016 ± 0.021
3.712 ± 0.024
3.755 ± 0.023
6.041 ± 0.026
5.966 ± 0.034
6.433 ± 0.024
1.202 ± 0.016
4.176 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here