Butyrivibrio virus Arian
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H1MR37|A0A6H1MR37_9CAUD Uncharacterized protein OS=Butyrivibrio virus Arian OX=2696357 PE=4 SV=1
MM1 pKa = 7.5 AAMEE5 pKa = 3.97 QSEE8 pKa = 4.41 ILTAVKK14 pKa = 10.44 NGLGITTDD22 pKa = 3.46 AQDD25 pKa = 3.12 ATLNVYY31 pKa = 9.9 IDD33 pKa = 3.87 EE34 pKa = 4.45 VQQFMIAAGVSEE46 pKa = 4.92 NVACDD51 pKa = 3.22 SCAIGCILRR60 pKa = 11.84 GVTDD64 pKa = 3.6 LWDD67 pKa = 3.91 YY68 pKa = 11.64 GSGSAGLSDD77 pKa = 4.0 YY78 pKa = 11.28 FKK80 pKa = 10.82 MRR82 pKa = 11.84 VTQLAMIKK90 pKa = 9.66 IDD92 pKa = 3.25
Molecular weight: 9.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.999
IPC_protein 3.897
Toseland 3.694
ProMoST 3.986
Dawson 3.91
Bjellqvist 4.139
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.846
Nozaki 4.037
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.024
Patrickios 1.926
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.974
Protein with the highest isoelectric point:
>tr|A0A6B9SX71|A0A6B9SX71_9CAUD Transposase/terminase small subunit OS=Butyrivibrio virus Arian OX=2696357 PE=4 SV=1
MM1 pKa = 7.29 FPAIYY6 pKa = 10.29 RR7 pKa = 11.84 FNQQGAGAMTIKK19 pKa = 9.95 EE20 pKa = 4.33 RR21 pKa = 11.84 KK22 pKa = 9.02 RR23 pKa = 11.84 LSDD26 pKa = 3.15 AKK28 pKa = 10.51 RR29 pKa = 11.84 YY30 pKa = 9.92 LKK32 pKa = 10.53 VRR34 pKa = 11.84 AADD37 pKa = 4.69 AIARR41 pKa = 11.84 GDD43 pKa = 3.83 LQYY46 pKa = 9.87 MQRR49 pKa = 11.84 IINDD53 pKa = 3.45 TVYY56 pKa = 10.7 FLDD59 pKa = 3.39 NVEE62 pKa = 4.08 YY63 pKa = 10.53 KK64 pKa = 10.81 GRR66 pKa = 11.84 SKK68 pKa = 9.69 TT69 pKa = 3.56
Molecular weight: 8.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.23
IPC2_protein 9.633
IPC_protein 9.999
Toseland 10.16
ProMoST 9.897
Dawson 10.379
Bjellqvist 10.058
Wikipedia 10.57
Rodwell 10.701
Grimsley 10.452
Solomon 10.409
Lehninger 10.379
Nozaki 10.116
DTASelect 10.072
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.277
Patrickios 10.394
IPC_peptide 10.409
IPC2_peptide 8.741
IPC2.peptide.svr19 8.691
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
10154
38
818
188.0
21.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.952 ± 0.728
1.211 ± 0.171
6.726 ± 0.314
6.106 ± 0.399
3.92 ± 0.288
6.726 ± 0.359
1.398 ± 0.187
7.406 ± 0.418
7.012 ± 0.472
6.874 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.63 ± 0.258
6.657 ± 0.284
2.551 ± 0.154
3.664 ± 0.238
4.215 ± 0.318
5.417 ± 0.424
7.406 ± 0.443
5.811 ± 0.241
1.172 ± 0.139
4.146 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here