Blastochloris viridis (Rhodopseudomonas viridis)
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3241 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N7IUI4|A0A0N7IUI4_BLAVI Cadherin-like beta sandwich domain protein OS=Blastochloris viridis OX=1079 GN=BVIRIDIS_11120 PE=4 SV=1
MM1 pKa = 6.61 TTVSSATSTTYY12 pKa = 10.81 SSSTSSSSSSLDD24 pKa = 3.47 LDD26 pKa = 3.62 TSDD29 pKa = 6.1 FLTLMCVQLQNQNPLDD45 pKa = 4.22 PSDD48 pKa = 3.48 PTEE51 pKa = 4.06 YY52 pKa = 9.91 MSQLVSYY59 pKa = 10.66 ASLSEE64 pKa = 3.84 QSEE67 pKa = 4.61 VNDD70 pKa = 3.23 QLGNITTALASLLAANAVGYY90 pKa = 10.1 VGHH93 pKa = 5.85 TVEE96 pKa = 5.7 AAGSTNALADD106 pKa = 4.7 GSASWNYY113 pKa = 9.19 TLASDD118 pKa = 4.45 ASKK121 pKa = 9.53 VTIKK125 pKa = 9.58 ITDD128 pKa = 3.5 EE129 pKa = 4.06 SGKK132 pKa = 8.37 TVYY135 pKa = 7.63 TTTGEE140 pKa = 4.22 TAAGEE145 pKa = 4.31 HH146 pKa = 6.09 SFVWDD151 pKa = 3.55 GTTSSGTKK159 pKa = 8.71 LTEE162 pKa = 3.8 GTYY165 pKa = 10.46 TMSITATDD173 pKa = 3.64 ADD175 pKa = 4.35 GNAVTTSSTVVGVVTGVDD193 pKa = 3.5 SSSGTVMLQIGDD205 pKa = 4.11 AEE207 pKa = 4.32 VAFANVIAIKK217 pKa = 10.55 SS218 pKa = 3.56
Molecular weight: 22.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.821
Patrickios 0.604
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.743
Protein with the highest isoelectric point:
>tr|A0A0S4Q6K6|A0A0S4Q6K6_BLAVI Uncharacterized protein OS=Blastochloris viridis OX=1079 GN=BVIRIDIS_31430 PE=4 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 KK3 pKa = 9.13 RR4 pKa = 11.84 RR5 pKa = 11.84 HH6 pKa = 4.77 GFRR9 pKa = 11.84 ARR11 pKa = 11.84 MATKK15 pKa = 10.31 SGRR18 pKa = 11.84 KK19 pKa = 9.29 VIALRR24 pKa = 11.84 RR25 pKa = 11.84 AQGRR29 pKa = 11.84 KK30 pKa = 9.22 RR31 pKa = 11.84 LSAA34 pKa = 3.93
Molecular weight: 4.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.618
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.34
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3241
0
3241
1038761
28
4528
320.5
34.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.156 ± 0.075
0.818 ± 0.014
5.709 ± 0.037
5.437 ± 0.037
3.579 ± 0.028
8.573 ± 0.046
1.928 ± 0.019
4.675 ± 0.034
2.89 ± 0.033
10.179 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.109 ± 0.022
2.182 ± 0.026
5.561 ± 0.041
2.747 ± 0.02
7.772 ± 0.048
4.903 ± 0.039
5.399 ± 0.048
8.163 ± 0.038
1.246 ± 0.018
1.97 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here