Kuenenia stuttgartiensis
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3801 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1PXJ0|Q1PXJ0_KUEST Phosphoesterase OS=Kuenenia stuttgartiensis OX=174633 GN=KsCSTR_41870 PE=3 SV=1
MM1 pKa = 7.48 EE2 pKa = 4.6 KK3 pKa = 9.9 WEE5 pKa = 4.59 CSVCGYY11 pKa = 10.95 VYY13 pKa = 10.59 DD14 pKa = 4.51 PEE16 pKa = 6.54 KK17 pKa = 10.86 GDD19 pKa = 3.98 PDD21 pKa = 3.71 NGVAPGTSFEE31 pKa = 4.79 SIQDD35 pKa = 3.37 DD36 pKa = 4.37 WVCPSCGANKK46 pKa = 10.45 DD47 pKa = 3.91 LFDD50 pKa = 5.35 KK51 pKa = 11.27 LDD53 pKa = 3.3
Molecular weight: 5.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.846
Dawson 3.757
Bjellqvist 3.999
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A2C9CD79|A0A2C9CD79_KUEST Uncharacterized protein OS=Kuenenia stuttgartiensis OX=174633 GN=KSMBR1_1034 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.53 VRR4 pKa = 11.84 IRR6 pKa = 11.84 KK7 pKa = 9.38 SSIKK11 pKa = 9.7 RR12 pKa = 11.84 KK13 pKa = 9.58 RR14 pKa = 11.84 MCGFRR19 pKa = 11.84 KK20 pKa = 9.96 RR21 pKa = 11.84 MRR23 pKa = 11.84 TKK25 pKa = 10.4 GGRR28 pKa = 11.84 AILNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 IGRR39 pKa = 11.84 RR40 pKa = 11.84 PLLNVV45 pKa = 3.2
Molecular weight: 5.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.47
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.457
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.179
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.105
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3801
0
3801
1118467
29
3219
294.3
33.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.757 ± 0.037
1.395 ± 0.021
5.108 ± 0.031
6.991 ± 0.045
4.557 ± 0.034
6.736 ± 0.048
2.118 ± 0.025
8.226 ± 0.042
7.61 ± 0.05
9.342 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.459 ± 0.018
4.668 ± 0.033
3.725 ± 0.027
2.991 ± 0.022
4.781 ± 0.034
6.103 ± 0.042
5.344 ± 0.061
6.42 ± 0.033
1.058 ± 0.016
3.609 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here