Alces alces faeces associated microvirus MP11 5517
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJ74|A0A2Z5CJ74_9VIRU Minor capsid protein OS=Alces alces faeces associated microvirus MP11 5517 OX=2219134 PE=4 SV=1
MM1 pKa = 7.66 AKK3 pKa = 10.28 FFTPYY8 pKa = 7.77 TTTKK12 pKa = 10.25 KK13 pKa = 10.6 VVVEE17 pKa = 4.98 FKK19 pKa = 10.73 EE20 pKa = 4.2 PSLTDD25 pKa = 3.04 QSYY28 pKa = 11.41 KK29 pKa = 10.83 DD30 pKa = 3.56 EE31 pKa = 5.25 CDD33 pKa = 2.97 LGFIIEE39 pKa = 4.5 NYY41 pKa = 8.12 VSKK44 pKa = 10.93 GIPLPQSTMNYY55 pKa = 9.31 QDD57 pKa = 3.56 CTTVQDD63 pKa = 3.98 YY64 pKa = 10.65 QSAMMLVAEE73 pKa = 4.8 AKK75 pKa = 10.74 SNFEE79 pKa = 3.89 QLPSKK84 pKa = 10.83 ARR86 pKa = 11.84 DD87 pKa = 3.28 EE88 pKa = 4.72 FGTVEE93 pKa = 4.23 NYY95 pKa = 10.93 LDD97 pKa = 4.99 FISKK101 pKa = 10.19 PEE103 pKa = 3.93 NLKK106 pKa = 9.39 TSYY109 pKa = 10.49 EE110 pKa = 3.88 KK111 pKa = 11.1 GYY113 pKa = 10.15 IDD115 pKa = 5.05 PSTVDD120 pKa = 4.0 LMDD123 pKa = 4.12 VYY125 pKa = 10.62 PEE127 pKa = 4.63 RR128 pKa = 11.84 YY129 pKa = 7.02 QTLSEE134 pKa = 4.17 QIEE137 pKa = 4.74 TPTVEE142 pKa = 4.1 PVVNPSEE149 pKa = 4.38 TPSTEE154 pKa = 3.64 VTAA157 pKa = 4.91
Molecular weight: 17.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.202
IPC2_protein 4.304
IPC_protein 4.215
Toseland 4.037
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.024
Rodwell 4.037
Grimsley 3.948
Solomon 4.151
Lehninger 4.101
Nozaki 4.266
DTASelect 4.406
Thurlkill 4.05
EMBOSS 4.037
Sillero 4.317
Patrickios 3.109
IPC_peptide 4.151
IPC2_peptide 4.304
IPC2.peptide.svr19 4.246
Protein with the highest isoelectric point:
>tr|A0A2Z5CHI1|A0A2Z5CHI1_9VIRU Nonstructural protein OS=Alces alces faeces associated microvirus MP11 5517 OX=2219134 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.32 RR3 pKa = 11.84 KK4 pKa = 9.2 QLSRR8 pKa = 11.84 KK9 pKa = 9.66 AGAKK13 pKa = 7.54 MFKK16 pKa = 10.3 RR17 pKa = 11.84 SGQMMNSKK25 pKa = 9.36 NQPKK29 pKa = 9.88 ISRR32 pKa = 11.84 GGIRR36 pKa = 11.84 FF37 pKa = 3.43
Molecular weight: 4.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.906
IPC_protein 12.442
Toseland 12.632
ProMoST 13.1
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.086
Rodwell 12.544
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.115
Sillero 12.632
Patrickios 12.281
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.991
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1296
37
542
216.0
24.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.093 ± 0.735
1.929 ± 0.878
5.71 ± 0.48
6.867 ± 1.052
4.475 ± 0.141
5.71 ± 0.988
2.006 ± 0.611
5.401 ± 0.665
7.407 ± 2.93
6.944 ± 0.371
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.009 ± 0.637
5.247 ± 0.497
4.475 ± 0.819
4.167 ± 1.021
4.244 ± 0.884
7.562 ± 1.04
6.096 ± 1.253
6.636 ± 0.896
1.157 ± 0.421
5.864 ± 0.922
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here