Roseibium sp. TrichSKD4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Stappiaceae; Roseibium; unclassified Roseibium

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5884 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E2CIE0|E2CIE0_9RHOB Ribonuclease T2 OS=Roseibium sp. TrichSKD4 OX=744980 GN=TRICHSKD4_2842 PE=3 SV=1
MM1 pKa = 7.69IGTNDD6 pKa = 3.97DD7 pKa = 4.95DD8 pKa = 3.99ILNGTVHH15 pKa = 6.91SDD17 pKa = 3.65VISGRR22 pKa = 11.84FGDD25 pKa = 4.13DD26 pKa = 3.01TLIGANGNDD35 pKa = 3.98EE36 pKa = 4.31VWGGTGDD43 pKa = 3.56DD44 pKa = 3.5TLYY47 pKa = 11.3GNNGNDD53 pKa = 3.01ILYY56 pKa = 10.83GSGGPNLVQITSVEE70 pKa = 3.91IGDD73 pKa = 4.75DD74 pKa = 3.91YY75 pKa = 11.19PVSVVFEE82 pKa = 4.55GEE84 pKa = 3.88TAGYY88 pKa = 10.2RR89 pKa = 11.84NTFGYY94 pKa = 10.72YY95 pKa = 9.52KK96 pKa = 10.34VSEE99 pKa = 5.1DD100 pKa = 3.02GTIYY104 pKa = 10.13HH105 pKa = 6.41VEE107 pKa = 4.41VIWPNASLQGSGGNLIQGEE126 pKa = 4.47SRR128 pKa = 11.84EE129 pKa = 4.05FLDD132 pKa = 4.29VQSGDD137 pKa = 3.27QLAFFIISNGYY148 pKa = 8.53SRR150 pKa = 11.84NGGYY154 pKa = 10.66AGIDD158 pKa = 3.65LEE160 pKa = 4.53NGTLEE165 pKa = 4.19FQEE168 pKa = 4.63SDD170 pKa = 3.1GTTATINSTSPRR182 pKa = 11.84LYY184 pKa = 10.81YY185 pKa = 10.02IAADD189 pKa = 3.87GTRR192 pKa = 11.84TMIQTDD198 pKa = 4.41PYY200 pKa = 8.86HH201 pKa = 5.58TAAYY205 pKa = 9.78GDD207 pKa = 4.09TVGLNPDD214 pKa = 3.91GFLHH218 pKa = 5.23TTGVLKK224 pKa = 10.13TDD226 pKa = 3.6AGTITLGFEE235 pKa = 4.06DD236 pKa = 5.82LYY238 pKa = 11.66NGGDD242 pKa = 3.5RR243 pKa = 11.84DD244 pKa = 3.72FDD246 pKa = 4.41DD247 pKa = 4.88SVFTVDD253 pKa = 4.39IGVANALVLNAHH265 pKa = 6.08YY266 pKa = 10.42RR267 pKa = 11.84GQNEE271 pKa = 4.6GEE273 pKa = 4.56DD274 pKa = 4.22PDD276 pKa = 4.42TGDD279 pKa = 3.43GEE281 pKa = 4.44NPVVIEE287 pKa = 4.79RR288 pKa = 11.84SDD290 pKa = 3.45NDD292 pKa = 3.2ILYY295 pKa = 10.59GGTGQDD301 pKa = 3.51EE302 pKa = 4.33LHH304 pKa = 6.47GRR306 pKa = 11.84SGDD309 pKa = 3.5DD310 pKa = 2.93TLYY313 pKa = 11.35GNNGEE318 pKa = 4.74DD319 pKa = 4.15DD320 pKa = 3.81LHH322 pKa = 7.4GGSGDD327 pKa = 3.53DD328 pKa = 3.5ALYY331 pKa = 10.85GGSASDD337 pKa = 3.54NLYY340 pKa = 11.13GNSGNDD346 pKa = 3.45TLFGGQGNDD355 pKa = 3.42SLNGNNGDD363 pKa = 4.75DD364 pKa = 3.71ILNGEE369 pKa = 4.47SGNDD373 pKa = 3.4EE374 pKa = 5.03LIGGTGNDD382 pKa = 4.01TLDD385 pKa = 3.91GGHH388 pKa = 6.79GNDD391 pKa = 4.48ILDD394 pKa = 3.87GEE396 pKa = 4.59YY397 pKa = 11.08GNDD400 pKa = 3.62VIKK403 pKa = 10.97GGAGDD408 pKa = 3.61DD409 pKa = 3.84QLRR412 pKa = 11.84GGYY415 pKa = 10.46NNDD418 pKa = 3.37EE419 pKa = 4.36LFGGHH424 pKa = 6.76GNDD427 pKa = 3.66TFYY430 pKa = 11.75GDD432 pKa = 3.47AGNDD436 pKa = 3.36VMHH439 pKa = 7.32GGRR442 pKa = 11.84GVDD445 pKa = 3.32TVDD448 pKa = 3.11YY449 pKa = 10.58SAYY452 pKa = 10.54DD453 pKa = 3.19VDD455 pKa = 4.62LTVSLHH461 pKa = 5.28NKK463 pKa = 8.12KK464 pKa = 10.3AHH466 pKa = 5.42GLEE469 pKa = 4.28IGSDD473 pKa = 3.44TLKK476 pKa = 11.06FIDD479 pKa = 5.28NITSGDD485 pKa = 3.44GDD487 pKa = 4.44DD488 pKa = 4.13FLKK491 pKa = 11.0GSSGDD496 pKa = 3.57NVINGGAGDD505 pKa = 3.74DD506 pKa = 4.27TIRR509 pKa = 11.84GLTGEE514 pKa = 4.49DD515 pKa = 4.06VLTGGAGADD524 pKa = 3.1TFVFRR529 pKa = 11.84TFDD532 pKa = 3.95LNPADD537 pKa = 4.52VITDD541 pKa = 4.3FDD543 pKa = 4.87LLNDD547 pKa = 4.09TLDD550 pKa = 3.84LSHH553 pKa = 7.21LAGGEE558 pKa = 4.3GEE560 pKa = 4.75GFLSSLQALEE570 pKa = 4.35QGDD573 pKa = 4.33GTLLSVDD580 pKa = 3.94LNGDD584 pKa = 3.34GSYY587 pKa = 11.2QDD589 pKa = 3.06ICSLEE594 pKa = 4.62GIGSVSLTTLNNNDD608 pKa = 3.61CFLFF612 pKa = 4.6

Molecular weight: 63.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2CK55|E2CK55_9RHOB Uncharacterized protein OS=Roseibium sp. TrichSKD4 OX=744980 GN=TRICHSKD4_3471 PE=4 SV=1
MM1 pKa = 7.33QFIADD6 pKa = 4.05WKK8 pKa = 10.21RR9 pKa = 11.84VFAVSLSFWTLVLGFLILLVPEE31 pKa = 3.86AVFRR35 pKa = 11.84FTGADD40 pKa = 3.37TNPYY44 pKa = 9.13LVGWFAVFLFVAGMVGRR61 pKa = 11.84FVKK64 pKa = 10.39QDD66 pKa = 2.86GSKK69 pKa = 9.79RR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.67WLRR75 pKa = 11.84ILGVVLGALVIAMALSGQGIAGQMAEE101 pKa = 4.13SRR103 pKa = 11.84EE104 pKa = 4.04AATLKK109 pKa = 10.45IAVPFIAAKK118 pKa = 9.35EE119 pKa = 4.28GKK121 pKa = 9.85RR122 pKa = 11.84NRR124 pKa = 11.84AYY126 pKa = 10.84LDD128 pKa = 3.46VVGVPTICYY137 pKa = 9.66GSTRR141 pKa = 11.84GVKK144 pKa = 10.32LGMVKK149 pKa = 9.72TNAEE153 pKa = 4.09CTALLRR159 pKa = 11.84DD160 pKa = 3.57EE161 pKa = 4.38VAEE164 pKa = 4.08YY165 pKa = 10.79RR166 pKa = 11.84HH167 pKa = 5.98GLHH170 pKa = 7.17PYY172 pKa = 7.71FTKK175 pKa = 8.39TTKK178 pKa = 9.98SRR180 pKa = 11.84RR181 pKa = 11.84LPPSRR186 pKa = 11.84DD187 pKa = 3.12AAFTSLAFNCGIRR200 pKa = 11.84AIGRR204 pKa = 11.84STATRR209 pKa = 11.84RR210 pKa = 11.84LNSGDD215 pKa = 3.03IRR217 pKa = 11.84GACHH221 pKa = 7.64AITWWNKK228 pKa = 8.51AGGRR232 pKa = 11.84VWRR235 pKa = 11.84GLVVRR240 pKa = 11.84RR241 pKa = 11.84SAEE244 pKa = 3.72RR245 pKa = 11.84DD246 pKa = 3.15LCLGNGTT253 pKa = 4.32

Molecular weight: 27.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5884

0

5884

1621200

17

6021

275.5

30.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.578 ± 0.043

0.926 ± 0.013

5.982 ± 0.05

6.313 ± 0.036

3.978 ± 0.023

8.114 ± 0.057

2.046 ± 0.016

5.422 ± 0.023

4.382 ± 0.033

9.9 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.491 ± 0.019

3.232 ± 0.025

4.702 ± 0.031

3.449 ± 0.019

6.126 ± 0.033

6.115 ± 0.034

5.49 ± 0.035

7.095 ± 0.027

1.316 ± 0.015

2.328 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski