Amino acid dipepetide frequency for Roseibium sp. TrichSKD4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.189AlaAla: 12.189 ± 0.119	1.052AlaCys: 1.052 ± 0.03	6.367AlaAsp: 6.367 ± 0.071	7.007AlaGlu: 7.007 ± 0.079	4.263AlaPhe: 4.263 ± 0.055	8.502AlaGly: 8.502 ± 0.128	2.056AlaHis: 2.056 ± 0.039	5.813AlaIle: 5.813 ± 0.072	4.635AlaLys: 4.635 ± 0.064	11.111AlaLeu: 11.111 ± 0.095	3.003AlaMet: 3.003 ± 0.043	3.322AlaAsn: 3.322 ± 0.048	4.359AlaPro: 4.359 ± 0.064	3.584AlaGln: 3.584 ± 0.047	6.231AlaArg: 6.231 ± 0.07	6.27AlaSer: 6.27 ± 0.071	5.07AlaThr: 5.07 ± 0.061	7.311AlaVal: 7.311 ± 0.082	1.218AlaTrp: 1.218 ± 0.029	2.421AlaTyr: 2.421 ± 0.035	0.001AlaXaa: 0.001 ± 0.001
Cys	0.946CysAla: 0.946 ± 0.026	0.131CysCys: 0.131 ± 0.01	0.572CysAsp: 0.572 ± 0.02	0.543CysGlu: 0.543 ± 0.019	0.369CysPhe: 0.369 ± 0.018	0.947CysGly: 0.947 ± 0.029	0.261CysHis: 0.261 ± 0.012	0.405CysIle: 0.405 ± 0.017	0.315CysLys: 0.315 ± 0.015	0.935CysLeu: 0.935 ± 0.027	0.191CysMet: 0.191 ± 0.012	0.27CysAsn: 0.27 ± 0.012	0.456CysPro: 0.456 ± 0.018	0.302CysGln: 0.302 ± 0.016	0.596CysArg: 0.596 ± 0.02	0.576CysSer: 0.576 ± 0.02	0.415CysThr: 0.415 ± 0.016	0.671CysVal: 0.671 ± 0.022	0.131CysTrp: 0.131 ± 0.009	0.233CysTyr: 0.233 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.068AspAla: 6.068 ± 0.065	0.509AspCys: 0.509 ± 0.018	3.43AspAsp: 3.43 ± 0.081	3.873AspGlu: 3.873 ± 0.054	2.393AspPhe: 2.393 ± 0.033	5.227AspGly: 5.227 ± 0.105	1.38AspHis: 1.38 ± 0.033	3.454AspIle: 3.454 ± 0.049	2.337AspLys: 2.337 ± 0.038	6.527AspLeu: 6.527 ± 0.077	1.503AspMet: 1.503 ± 0.031	1.587AspAsn: 1.587 ± 0.039	3.274AspPro: 3.274 ± 0.049	2.148AspGln: 2.148 ± 0.038	3.91AspArg: 3.91 ± 0.054	2.12AspSer: 2.12 ± 0.036	3.182AspThr: 3.182 ± 0.177	4.232AspVal: 4.232 ± 0.056	1.041AspTrp: 1.041 ± 0.025	1.627AspTyr: 1.627 ± 0.032	0.001AspXaa: 0.001 ± 0.001
Glu	7.364GluAla: 7.364 ± 0.077	0.458GluCys: 0.458 ± 0.016	3.646GluAsp: 3.646 ± 0.055	4.067GluGlu: 4.067 ± 0.06	2.207GluPhe: 2.207 ± 0.04	4.543GluGly: 4.543 ± 0.063	1.24GluHis: 1.24 ± 0.03	3.975GluIle: 3.975 ± 0.056	3.231GluLys: 3.231 ± 0.05	6.054GluLeu: 6.054 ± 0.07	1.601GluMet: 1.601 ± 0.035	2.456GluAsn: 2.456 ± 0.039	2.54GluPro: 2.54 ± 0.057	2.489GluGln: 2.489 ± 0.044	4.479GluArg: 4.479 ± 0.061	2.437GluSer: 2.437 ± 0.038	4.301GluThr: 4.301 ± 0.059	4.176GluVal: 4.176 ± 0.056	0.704GluTrp: 0.704 ± 0.023	1.161GluTyr: 1.161 ± 0.025	0.001GluXaa: 0.001 ± 0.001
Phe	4.187PheAla: 4.187 ± 0.053	0.495PheCys: 0.495 ± 0.02	2.779PheAsp: 2.779 ± 0.045	2.652PheGlu: 2.652 ± 0.039	1.584PhePhe: 1.584 ± 0.04	3.751PheGly: 3.751 ± 0.06	0.787PheHis: 0.787 ± 0.025	1.86PheIle: 1.86 ± 0.036	1.572PheLys: 1.572 ± 0.031	3.779PheLeu: 3.779 ± 0.061	0.903PheMet: 0.903 ± 0.025	1.241PheAsn: 1.241 ± 0.03	1.622PhePro: 1.622 ± 0.033	1.333PheGln: 1.333 ± 0.03	2.123PheArg: 2.123 ± 0.035	2.681PheSer: 2.681 ± 0.045	1.918PheThr: 1.918 ± 0.04	2.915PheVal: 2.915 ± 0.049	0.621PheTrp: 0.621 ± 0.021	0.98PheTyr: 0.98 ± 0.026	0.001PheXaa: 0.001 ± 0.001
Gly	7.694GlyAla: 7.694 ± 0.127	0.838GlyCys: 0.838 ± 0.024	4.473GlyAsp: 4.473 ± 0.15	5.014GlyGlu: 5.014 ± 0.072	3.647GlyPhe: 3.647 ± 0.05	6.933GlyGly: 6.933 ± 0.204	1.831GlyHis: 1.831 ± 0.037	4.306GlyIle: 4.306 ± 0.058	3.909GlyLys: 3.909 ± 0.056	8.343GlyLeu: 8.343 ± 0.087	2.127GlyMet: 2.127 ± 0.038	2.56GlyAsn: 2.56 ± 0.064	3.001GlyPro: 3.001 ± 0.051	2.996GlyGln: 2.996 ± 0.047	4.817GlyArg: 4.817 ± 0.067	4.864GlySer: 4.864 ± 0.09	4.422GlyThr: 4.422 ± 0.074	5.671GlyVal: 5.671 ± 0.073	1.292GlyTrp: 1.292 ± 0.047	2.394GlyTyr: 2.394 ± 0.038	0.002GlyXaa: 0.002 ± 0.001
His	1.926HisAla: 1.926 ± 0.036	0.251HisCys: 0.251 ± 0.012	1.247HisAsp: 1.247 ± 0.029	1.127HisGlu: 1.127 ± 0.026	0.921HisPhe: 0.921 ± 0.023	1.741HisGly: 1.741 ± 0.034	0.579HisHis: 0.579 ± 0.022	1.062HisIle: 1.062 ± 0.03	0.773HisLys: 0.773 ± 0.024	2.171HisLeu: 2.171 ± 0.041	0.545HisMet: 0.545 ± 0.022	0.551HisAsn: 0.551 ± 0.022	1.22HisPro: 1.22 ± 0.03	0.706HisGln: 0.706 ± 0.019	1.255HisArg: 1.255 ± 0.029	1.096HisSer: 1.096 ± 0.028	0.936HisThr: 0.936 ± 0.024	1.493HisVal: 1.493 ± 0.033	0.304HisTrp: 0.304 ± 0.014	0.561HisTyr: 0.561 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.387IleAla: 6.387 ± 0.077	0.635IleCys: 0.635 ± 0.018	3.696IleAsp: 3.696 ± 0.059	3.866IleGlu: 3.866 ± 0.048	1.905IlePhe: 1.905 ± 0.041	4.925IleGly: 4.925 ± 0.063	0.939IleHis: 0.939 ± 0.025	2.515IleIle: 2.515 ± 0.045	2.059IleLys: 2.059 ± 0.035	4.867IleLeu: 4.867 ± 0.064	1.12IleMet: 1.12 ± 0.029	1.748IleAsn: 1.748 ± 0.036	2.344IlePro: 2.344 ± 0.04	1.562IleGln: 1.562 ± 0.033	3.222IleArg: 3.222 ± 0.051	3.746IleSer: 3.746 ± 0.057	2.856IleThr: 2.856 ± 0.045	3.878IleVal: 3.878 ± 0.053	0.696IleTrp: 0.696 ± 0.023	1.256IleTyr: 1.256 ± 0.032	0.001IleXaa: 0.001 ± 0.001
Lys	5.071LysAla: 5.071 ± 0.066	0.265LysCys: 0.265 ± 0.014	2.49LysAsp: 2.49 ± 0.042	2.36LysGlu: 2.36 ± 0.045	1.301LysPhe: 1.301 ± 0.029	3.235LysGly: 3.235 ± 0.049	0.873LysHis: 0.873 ± 0.027	2.361LysIle: 2.361 ± 0.042	2.044LysLys: 2.044 ± 0.044	4.192LysLeu: 4.192 ± 0.058	1.048LysMet: 1.048 ± 0.026	1.476LysAsn: 1.476 ± 0.036	2.403LysPro: 2.403 ± 0.041	1.508LysGln: 1.508 ± 0.034	2.997LysArg: 2.997 ± 0.054	2.796LysSer: 2.796 ± 0.043	2.877LysThr: 2.877 ± 0.043	3.164LysVal: 3.164 ± 0.048	0.503LysTrp: 0.503 ± 0.021	0.856LysTyr: 0.856 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	10.851LeuAla: 10.851 ± 0.091	0.925LeuCys: 0.925 ± 0.028	5.969LeuAsp: 5.969 ± 0.074	6.302LeuGlu: 6.302 ± 0.067	3.847LeuPhe: 3.847 ± 0.056	7.63LeuGly: 7.63 ± 0.072	1.799LeuHis: 1.799 ± 0.035	5.209LeuIle: 5.209 ± 0.065	4.962LeuLys: 4.962 ± 0.059	8.795LeuLeu: 8.795 ± 0.083	2.299LeuMet: 2.299 ± 0.035	3.3LeuAsn: 3.3 ± 0.046	5.066LeuPro: 5.066 ± 0.055	3.137LeuGln: 3.137 ± 0.05	5.751LeuArg: 5.751 ± 0.07	7.431LeuSer: 7.431 ± 0.076	5.705LeuThr: 5.705 ± 0.064	6.864LeuVal: 6.864 ± 0.072	1.128LeuTrp: 1.128 ± 0.03	2.029LeuTyr: 2.029 ± 0.039	0.001LeuXaa: 0.001 ± 0.001
Met	2.795MetAla: 2.795 ± 0.047	0.197MetCys: 0.197 ± 0.011	1.3MetAsp: 1.3 ± 0.03	1.33MetGlu: 1.33 ± 0.031	0.777MetPhe: 0.777 ± 0.023	1.781MetGly: 1.781 ± 0.037	0.431MetHis: 0.431 ± 0.017	1.422MetIle: 1.422 ± 0.036	1.21MetLys: 1.21 ± 0.028	2.266MetLeu: 2.266 ± 0.034	0.685MetMet: 0.685 ± 0.024	0.9MetAsn: 0.9 ± 0.025	1.393MetPro: 1.393 ± 0.032	0.812MetGln: 0.812 ± 0.022	1.622MetArg: 1.622 ± 0.034	1.869MetSer: 1.869 ± 0.034	1.844MetThr: 1.844 ± 0.037	1.725MetVal: 1.725 ± 0.035	0.228MetTrp: 0.228 ± 0.011	0.32MetTyr: 0.32 ± 0.015	0.001MetXaa: 0.001 ± 0.001
Asn	3.444AsnAla: 3.444 ± 0.052	0.288AsnCys: 0.288 ± 0.013	1.865AsnAsp: 1.865 ± 0.062	1.827AsnGlu: 1.827 ± 0.033	1.142AsnPhe: 1.142 ± 0.028	3.016AsnGly: 3.016 ± 0.068	0.625AsnHis: 0.625 ± 0.023	1.664AsnIle: 1.664 ± 0.028	1.211AsnLys: 1.211 ± 0.03	3.101AsnLeu: 3.101 ± 0.048	0.804AsnMet: 0.804 ± 0.024	1.065AsnAsn: 1.065 ± 0.056	2.061AsnPro: 2.061 ± 0.043	1.087AsnGln: 1.087 ± 0.026	2.162AsnArg: 2.162 ± 0.041	1.778AsnSer: 1.778 ± 0.038	1.663AsnThr: 1.663 ± 0.034	2.21AsnVal: 2.21 ± 0.046	0.543AsnTrp: 0.543 ± 0.022	0.761AsnTyr: 0.761 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	4.538ProAla: 4.538 ± 0.065	0.366ProCys: 0.366 ± 0.015	3.538ProAsp: 3.538 ± 0.048	3.773ProGlu: 3.773 ± 0.054	2.023ProPhe: 2.023 ± 0.04	3.546ProGly: 3.546 ± 0.052	0.977ProHis: 0.977 ± 0.026	2.428ProIle: 2.428 ± 0.039	2.234ProLys: 2.234 ± 0.04	4.168ProLeu: 4.168 ± 0.057	1.123ProMet: 1.123 ± 0.03	1.638ProAsn: 1.638 ± 0.036	1.945ProPro: 1.945 ± 0.04	1.53ProGln: 1.53 ± 0.036	2.251ProArg: 2.251 ± 0.045	2.844ProSer: 2.844 ± 0.039	2.435ProThr: 2.435 ± 0.035	3.868ProVal: 3.868 ± 0.058	0.613ProTrp: 0.613 ± 0.022	1.181ProTyr: 1.181 ± 0.03	0.002ProXaa: 0.002 ± 0.001
Gln	3.694GlnAla: 3.694 ± 0.053	0.221GlnCys: 0.221 ± 0.013	1.741GlnAsp: 1.741 ± 0.031	1.947GlnGlu: 1.947 ± 0.043	1.277GlnPhe: 1.277 ± 0.027	2.296GlnGly: 2.296 ± 0.04	0.716GlnHis: 0.716 ± 0.02	2.247GlnIle: 2.247 ± 0.038	1.663GlnLys: 1.663 ± 0.038	3.3GlnLeu: 3.3 ± 0.047	1.06GlnMet: 1.06 ± 0.028	1.319GlnAsn: 1.319 ± 0.033	1.548GlnPro: 1.548 ± 0.03	1.253GlnGln: 1.253 ± 0.04	2.275GlnArg: 2.275 ± 0.04	2.277GlnSer: 2.277 ± 0.041	2.197GlnThr: 2.197 ± 0.033	2.387GlnVal: 2.387 ± 0.038	0.408GlnTrp: 0.408 ± 0.015	0.658GlnTyr: 0.658 ± 0.022	0.001GlnXaa: 0.001 ± 0.001
Arg	5.78ArgAla: 5.78 ± 0.068	0.501ArgCys: 0.501 ± 0.02	3.459ArgAsp: 3.459 ± 0.049	3.829ArgGlu: 3.829 ± 0.053	2.813ArgPhe: 2.813 ± 0.044	4.001ArgGly: 4.001 ± 0.051	1.414ArgHis: 1.414 ± 0.032	3.512ArgIle: 3.512 ± 0.051	2.943ArgLys: 2.943 ± 0.048	6.733ArgLeu: 6.733 ± 0.092	1.581ArgMet: 1.581 ± 0.035	1.854ArgAsn: 1.854 ± 0.036	2.852ArgPro: 2.852 ± 0.05	2.507ArgGln: 2.507 ± 0.042	4.2ArgArg: 4.2 ± 0.062	3.711ArgSer: 3.711 ± 0.049	3.119ArgThr: 3.119 ± 0.039	4.069ArgVal: 4.069 ± 0.058	0.807ArgTrp: 0.807 ± 0.021	1.578ArgTyr: 1.578 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	6.369SerAla: 6.369 ± 0.079	0.512SerCys: 0.512 ± 0.019	3.811SerAsp: 3.811 ± 0.054	3.92SerGlu: 3.92 ± 0.052	2.57SerPhe: 2.57 ± 0.046	5.917SerGly: 5.917 ± 0.101	1.217SerHis: 1.217 ± 0.03	3.183SerIle: 3.183 ± 0.048	2.502SerLys: 2.502 ± 0.04	5.833SerLeu: 5.833 ± 0.063	1.488SerMet: 1.488 ± 0.032	2.015SerAsn: 2.015 ± 0.04	2.692SerPro: 2.692 ± 0.048	2.069SerGln: 2.069 ± 0.042	3.487SerArg: 3.487 ± 0.05	3.861SerSer: 3.861 ± 0.067	3.037SerThr: 3.037 ± 0.046	4.391SerVal: 4.391 ± 0.058	0.812SerTrp: 0.812 ± 0.022	1.461SerTyr: 1.461 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	5.743ThrAla: 5.743 ± 0.06	0.497ThrCys: 0.497 ± 0.016	3.097ThrAsp: 3.097 ± 0.049	3.065ThrGlu: 3.065 ± 0.046	2.162ThrPhe: 2.162 ± 0.036	5.07ThrGly: 5.07 ± 0.1	1.104ThrHis: 1.104 ± 0.027	3.138ThrIle: 3.138 ± 0.069	2.092ThrLys: 2.092 ± 0.036	5.741ThrLeu: 5.741 ± 0.107	1.182ThrMet: 1.182 ± 0.026	1.691ThrAsn: 1.691 ± 0.034	3.043ThrPro: 3.043 ± 0.045	1.707ThrGln: 1.707 ± 0.034	3.074ThrArg: 3.074 ± 0.046	3.383ThrSer: 3.383 ± 0.053	2.892ThrThr: 2.892 ± 0.055	4.206ThrVal: 4.206 ± 0.059	0.661ThrTrp: 0.661 ± 0.02	1.352ThrTyr: 1.352 ± 0.028	0.002ThrXaa: 0.002 ± 0.001
Val	7.371ValAla: 7.371 ± 0.081	0.685ValCys: 0.685 ± 0.019	4.197ValAsp: 4.197 ± 0.054	4.565ValGlu: 4.565 ± 0.053	2.962ValPhe: 2.962 ± 0.047	5.138ValGly: 5.138 ± 0.059	1.343ValHis: 1.343 ± 0.031	3.908ValIle: 3.908 ± 0.059	2.718ValLys: 2.718 ± 0.046	7.194ValLeu: 7.194 ± 0.069	1.787ValMet: 1.787 ± 0.034	2.132ValAsn: 2.132 ± 0.044	3.456ValPro: 3.456 ± 0.051	2.263ValGln: 2.263 ± 0.037	4.237ValArg: 4.237 ± 0.054	5.089ValSer: 5.089 ± 0.055	4.19ValThr: 4.19 ± 0.062	5.318ValVal: 5.318 ± 0.069	0.855ValTrp: 0.855 ± 0.023	1.543ValTyr: 1.543 ± 0.031	0.001ValXaa: 0.001 ± 0.001
Trp	1.13TrpAla: 1.13 ± 0.027	0.16TrpCys: 0.16 ± 0.01	0.682TrpAsp: 0.682 ± 0.023	0.621TrpGlu: 0.621 ± 0.021	0.566TrpPhe: 0.566 ± 0.019	0.899TrpGly: 0.899 ± 0.025	0.353TrpHis: 0.353 ± 0.015	0.727TrpIle: 0.727 ± 0.02	0.535TrpLys: 0.535 ± 0.019	1.485TrpLeu: 1.485 ± 0.034	0.372TrpMet: 0.372 ± 0.018	0.509TrpAsn: 0.509 ± 0.037	0.656TrpPro: 0.656 ± 0.019	0.582TrpGln: 0.582 ± 0.018	0.93TrpArg: 0.93 ± 0.024	0.874TrpSer: 0.874 ± 0.023	0.724TrpThr: 0.724 ± 0.022	0.849TrpVal: 0.849 ± 0.028	0.212TrpTrp: 0.212 ± 0.012	0.299TrpTyr: 0.299 ± 0.014	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.236TyrAla: 2.236 ± 0.039	0.278TyrCys: 0.278 ± 0.014	1.464TyrAsp: 1.464 ± 0.027	1.44TyrGlu: 1.44 ± 0.031	1.053TyrPhe: 1.053 ± 0.025	2.037TyrGly: 2.037 ± 0.044	0.538TyrHis: 0.538 ± 0.019	1.033TyrIle: 1.033 ± 0.027	0.875TyrLys: 0.875 ± 0.023	2.403TyrLeu: 2.403 ± 0.044	0.487TyrMet: 0.487 ± 0.018	0.682TyrAsn: 0.682 ± 0.021	1.087TyrPro: 1.087 ± 0.026	0.911TyrGln: 0.911 ± 0.025	1.644TyrArg: 1.644 ± 0.03	1.442TyrSer: 1.442 ± 0.035	1.119TyrThr: 1.119 ± 0.024	1.556TyrVal: 1.556 ± 0.034	0.388TyrTrp: 0.388 ± 0.019	0.606TyrTyr: 0.606 ± 0.02	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.124XaaXaa: 0.124 ± 0.027

Statistics based on 5884 proteins (1621200 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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