Fuerstia marisgermanicae
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6466 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8WLJ7|A0A1P8WLJ7_9PLAN Uncharacterized protein OS=Fuerstia marisgermanicae OX=1891926 GN=Fuma_04588 PE=4 SV=1
MM1 pKa = 7.33 LQTSNSAGEE10 pKa = 4.11 LGAEE14 pKa = 4.37 VVLSSLSSIASTKK27 pKa = 10.64 LHH29 pKa = 7.31 DD30 pKa = 4.23 IDD32 pKa = 5.34 NDD34 pKa = 3.57 GDD36 pKa = 4.22 LDD38 pKa = 3.98 IVASDD43 pKa = 3.89 SGAEE47 pKa = 4.13 SILIFINDD55 pKa = 3.55 GSANFGAPASVTPDD69 pKa = 3.11 EE70 pKa = 4.85 TGTTLHH76 pKa = 6.19 QVVDD80 pKa = 4.52 LNGDD84 pKa = 4.01 GYY86 pKa = 11.86 ADD88 pKa = 5.03 LLVTDD93 pKa = 5.09 AGAPNKK99 pKa = 9.74 VGYY102 pKa = 9.95 YY103 pKa = 8.0 PQQAGGTFGARR114 pKa = 11.84 VNVPIYY120 pKa = 9.5 GQVVSSLSATDD131 pKa = 3.87 FNGDD135 pKa = 3.3 GVIEE139 pKa = 4.3 IGFAHH144 pKa = 6.6 TNYY147 pKa = 10.67 DD148 pKa = 3.46 DD149 pKa = 3.63 VAFTFFDD156 pKa = 4.86 FNVGWALGQGGGDD169 pKa = 4.11 FGPAIVVQRR178 pKa = 11.84 YY179 pKa = 9.37 DD180 pKa = 3.29 SIMYY184 pKa = 10.93 SMATPDD190 pKa = 4.2 LDD192 pKa = 4.88 GDD194 pKa = 4.06 GDD196 pKa = 4.22 PDD198 pKa = 3.32 IVVPQRR204 pKa = 11.84 SGPTSVAAFINQDD217 pKa = 3.07 GEE219 pKa = 4.17 DD220 pKa = 3.9 PMRR223 pKa = 11.84 FIAPEE228 pKa = 3.71 SRR230 pKa = 11.84 TYY232 pKa = 10.58 TGGDD236 pKa = 3.61 PIEE239 pKa = 4.19 LQVYY243 pKa = 8.76 FGLPITVTGTPRR255 pKa = 11.84 IALDD259 pKa = 3.59 LGGEE263 pKa = 4.34 TVYY266 pKa = 11.22 AEE268 pKa = 4.25 YY269 pKa = 10.95 ASGSGTATHH278 pKa = 5.8 QFVYY282 pKa = 10.44 QVGEE286 pKa = 4.05 TDD288 pKa = 3.79 LDD290 pKa = 4.17 LDD292 pKa = 4.46 GVQLADD298 pKa = 3.64 NAIDD302 pKa = 4.1 LNEE305 pKa = 4.28 GTLTDD310 pKa = 4.15 PLGTSALLEE319 pKa = 4.53 FPDD322 pKa = 3.97 TVLDD326 pKa = 4.03 GVVVNAVGPLVQHH339 pKa = 7.03 ISRR342 pKa = 11.84 GDD344 pKa = 3.62 SAPTDD349 pKa = 3.44 AASVRR354 pKa = 11.84 FTVEE358 pKa = 3.29 FSEE361 pKa = 4.37 AVEE364 pKa = 4.34 GVDD367 pKa = 4.63 VSDD370 pKa = 4.53 FQVKK374 pKa = 8.48 MFDD377 pKa = 3.47 GDD379 pKa = 3.64 LAGAVIEE386 pKa = 4.62 SVTGSGGTYY395 pKa = 9.72 EE396 pKa = 4.36 VTVSAGTGSGVLALSVNEE414 pKa = 4.17 NASIADD420 pKa = 3.86 LEE422 pKa = 4.41 GAPLARR428 pKa = 11.84 GYY430 pKa = 11.04 VGGEE434 pKa = 4.08 VYY436 pKa = 9.85 TLRR439 pKa = 11.84 RR440 pKa = 11.84 GVEE443 pKa = 3.94 APIDD447 pKa = 3.51 KK448 pKa = 10.66 YY449 pKa = 10.49 FTDD452 pKa = 2.9 GHH454 pKa = 6.07 GDD456 pKa = 3.53 YY457 pKa = 10.94 RR458 pKa = 11.84 PVLNDD463 pKa = 3.58 GEE465 pKa = 4.38 LSYY468 pKa = 11.31 VLHH471 pKa = 7.06 GDD473 pKa = 3.34 SGVIPDD479 pKa = 4.55 GEE481 pKa = 4.46 VPSGGIYY488 pKa = 9.88 TYY490 pKa = 11.16 VDD492 pKa = 2.82 STGIVNRR499 pKa = 11.84 AEE501 pKa = 4.15 DD502 pKa = 3.78 DD503 pKa = 3.71 SYY505 pKa = 12.24 DD506 pKa = 3.84 FLGVDD511 pKa = 3.86 ANEE514 pKa = 4.1 PLYY517 pKa = 10.44 VIPSTQVPSLPFLGFSGEE535 pKa = 4.35 GVPSGALASYY545 pKa = 10.86 VPDD548 pKa = 3.75 RR549 pKa = 11.84 PNVTSSSASPYY560 pKa = 10.18 IKK562 pKa = 10.61 LEE564 pKa = 4.05 MVDD567 pKa = 3.53 VRR569 pKa = 11.84 STSGGEE575 pKa = 3.6 FSLWSNPPSWNPSAGVTVYY594 pKa = 9.6 MATSDD599 pKa = 3.89 GVSDD603 pKa = 4.31 ADD605 pKa = 3.89 ALFLRR610 pKa = 11.84 VGSHH614 pKa = 4.51 GHH616 pKa = 5.62 FNVGFSEE623 pKa = 4.15 PGIYY627 pKa = 10.09 EE628 pKa = 3.72 VDD630 pKa = 3.54 IYY632 pKa = 11.68 ASGYY636 pKa = 10.24 LDD638 pKa = 3.74 NDD640 pKa = 3.76 HH641 pKa = 6.93 NGVYY645 pKa = 10.56 DD646 pKa = 4.07 PGKK649 pKa = 10.37 DD650 pKa = 3.27 SYY652 pKa = 11.12 IEE654 pKa = 4.31 SGIQTMVFAVDD665 pKa = 3.23 SLGAVDD671 pKa = 6.02 DD672 pKa = 4.38 SFTVLEE678 pKa = 4.71 GEE680 pKa = 4.46 TLTGDD685 pKa = 4.15 VSANDD690 pKa = 3.72 DD691 pKa = 2.83 WHH693 pKa = 6.38 EE694 pKa = 4.17 AMGEE698 pKa = 4.05 YY699 pKa = 8.61 TASVEE704 pKa = 4.15 TEE706 pKa = 4.07 PAHH709 pKa = 6.48 GTLTLNADD717 pKa = 3.44 GTFTYY722 pKa = 10.5 EE723 pKa = 3.76 PVAALTGVDD732 pKa = 3.22 SFAYY736 pKa = 10.32 RR737 pKa = 11.84 LTNEE741 pKa = 4.01 RR742 pKa = 11.84 GGFTTATVTLLTHH755 pKa = 6.71 HH756 pKa = 6.83 SPTAVEE762 pKa = 4.17 DD763 pKa = 4.06 AFVVASGSSVYY774 pKa = 11.11 GNVLFNDD781 pKa = 3.93 FDD783 pKa = 4.37 VDD785 pKa = 4.06 GDD787 pKa = 4.18 ALLAALDD794 pKa = 4.34 AGPTSGSVVVSADD807 pKa = 2.83 GSFVYY812 pKa = 10.17 TPSAAFDD819 pKa = 4.32 DD820 pKa = 4.58 SDD822 pKa = 4.06 SFTYY826 pKa = 10.68 SIDD829 pKa = 3.85 DD830 pKa = 3.81 GTGLASTATVVISAAEE846 pKa = 3.87 DD847 pKa = 3.6 AEE849 pKa = 4.46 FEE851 pKa = 4.51 VVLTEE856 pKa = 3.7 GHH858 pKa = 5.63 VDD860 pKa = 2.81 IGVVIGEE867 pKa = 4.39 HH868 pKa = 6.75 DD869 pKa = 3.79 HH870 pKa = 8.13 DD871 pKa = 4.85 EE872 pKa = 5.59 DD873 pKa = 5.71 EE874 pKa = 5.95 DD875 pKa = 4.48 DD876 pKa = 5.77 HH877 pKa = 9.71 DD878 pKa = 6.91 DD879 pKa = 5.8 DD880 pKa = 6.59 DD881 pKa = 5.3 DD882 pKa = 5.26 HH883 pKa = 8.94 DD884 pKa = 4.84 HH885 pKa = 6.87 EE886 pKa = 5.98 EE887 pKa = 3.8 EE888 pKa = 4.68 AEE890 pKa = 4.08 WDD892 pKa = 3.79 LHH894 pKa = 5.27 VHH896 pKa = 4.91 DD897 pKa = 5.39 HH898 pKa = 6.08 EE899 pKa = 6.24 GEE901 pKa = 4.32 VEE903 pKa = 4.08 YY904 pKa = 10.64 HH905 pKa = 6.73 ADD907 pKa = 3.47 EE908 pKa = 4.66 ALLYY912 pKa = 10.91 VGMDD916 pKa = 4.81 AITDD920 pKa = 4.04 RR921 pKa = 11.84 PAGTAYY927 pKa = 10.74 DD928 pKa = 4.88 FIGTDD933 pKa = 2.92 AGEE936 pKa = 4.52 SVFVLPATEE945 pKa = 4.4 NSDD948 pKa = 4.62 LLFLGLGTEE957 pKa = 4.35 EE958 pKa = 4.53 IEE960 pKa = 4.61 VGTFLDD966 pKa = 3.95 GTLEE970 pKa = 3.96 LRR972 pKa = 11.84 LKK974 pKa = 10.49 SVSGPGQFSMWTSGLEE990 pKa = 4.14 GPEE993 pKa = 3.79 VAMATSDD1000 pKa = 4.21 GITEE1004 pKa = 4.52 ADD1006 pKa = 4.84 LITLLEE1012 pKa = 4.73 AGHH1015 pKa = 6.26 AHH1017 pKa = 6.62 ANFGFTEE1024 pKa = 3.96 MGLYY1028 pKa = 10.28 QITLQASGTLADD1040 pKa = 4.24 GDD1042 pKa = 3.81 VSEE1045 pKa = 4.91 DD1046 pKa = 3.01 VTYY1049 pKa = 10.39 FFKK1052 pKa = 11.09 VGNTAEE1058 pKa = 4.74 GIEE1061 pKa = 4.32 VQNGMTQRR1069 pKa = 11.84 SYY1071 pKa = 11.17 IDD1073 pKa = 3.49 EE1074 pKa = 4.91 LDD1076 pKa = 3.66 VLFGSEE1082 pKa = 5.5 DD1083 pKa = 3.64 GLDD1086 pKa = 4.82 DD1087 pKa = 4.64 ILTNPDD1093 pKa = 2.66 RR1094 pKa = 11.84 VQITKK1099 pKa = 10.52 YY1100 pKa = 10.68 DD1101 pKa = 3.86 LNGEE1105 pKa = 4.21 NGSLLSDD1112 pKa = 3.61 AGYY1115 pKa = 10.69 SLSANGSRR1123 pKa = 11.84 LSFNFGVQGLGGNRR1137 pKa = 11.84 NSNAGDD1143 pKa = 3.84 GYY1145 pKa = 10.94 YY1146 pKa = 10.29 EE1147 pKa = 4.17 IGIDD1151 pKa = 3.47 ADD1153 pKa = 3.84 GDD1155 pKa = 4.23 GEE1157 pKa = 4.55 YY1158 pKa = 9.11 EE1159 pKa = 4.46 TYY1161 pKa = 10.59 QSFYY1165 pKa = 11.27 RR1166 pKa = 11.84 LLGDD1170 pKa = 3.31 VNGDD1174 pKa = 3.68 RR1175 pKa = 11.84 KK1176 pKa = 10.53 VDD1178 pKa = 3.24 AADD1181 pKa = 3.76 RR1182 pKa = 11.84 MSVMQSMRR1190 pKa = 11.84 NQNPEE1195 pKa = 3.59 ADD1197 pKa = 3.8 VNGDD1201 pKa = 3.19 GRR1203 pKa = 11.84 VNSIDD1208 pKa = 3.5 FVLVSRR1214 pKa = 11.84 ALGRR1218 pKa = 11.84 DD1219 pKa = 3.26 LRR1221 pKa = 11.84 DD1222 pKa = 3.24 GLFDD1226 pKa = 5.63 DD1227 pKa = 6.27 DD1228 pKa = 4.63 EE1229 pKa = 6.9 DD1230 pKa = 4.0 EE1231 pKa = 4.25
Molecular weight: 129.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.465
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.516
Grimsley 3.376
Solomon 3.694
Lehninger 3.656
Nozaki 3.795
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.643
Sillero 3.821
Patrickios 1.049
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.738
Protein with the highest isoelectric point:
>tr|A0A1P8WL87|A0A1P8WL87_9PLAN Uncharacterized protein OS=Fuerstia marisgermanicae OX=1891926 GN=Fuma_04454 PE=4 SV=1
MM1 pKa = 7.1 QRR3 pKa = 11.84 AKK5 pKa = 10.82 LNPPPKK11 pKa = 9.25 TNLVSLLISTFIGAVTGAVVLAPFTRR37 pKa = 11.84 SFGAPTGRR45 pKa = 11.84 GVGFGLGGMISMIGALIVRR64 pKa = 11.84 GLGRR68 pKa = 11.84 RR69 pKa = 11.84 LTLL72 pKa = 3.73
Molecular weight: 7.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.441
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6466
0
6466
2532177
30
12079
391.6
43.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.809 ± 0.034
1.15 ± 0.018
6.501 ± 0.044
5.894 ± 0.04
3.811 ± 0.017
7.61 ± 0.059
2.241 ± 0.021
4.881 ± 0.028
3.782 ± 0.047
9.409 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.182 ± 0.021
3.55 ± 0.036
5.001 ± 0.029
4.035 ± 0.028
6.278 ± 0.053
6.63 ± 0.034
6.115 ± 0.077
7.387 ± 0.028
1.466 ± 0.015
2.267 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here