Methanobacterium sp. A39

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobacterium; unclassified Methanobacterium

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3065 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D2W4D5|A0A1D2W4D5_9EURY Uncharacterized protein OS=Methanobacterium sp. A39 OX=1860100 GN=A9507_15755 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.28KK3 pKa = 10.41VLVLFLTIAVVAGTSIGFGATLNSNTNDD31 pKa = 3.48PGNTINTAANDD42 pKa = 3.79GSNIDD47 pKa = 3.52NSQNDD52 pKa = 3.9GNGDD56 pKa = 3.89NINQNRR62 pKa = 11.84DD63 pKa = 3.35DD64 pKa = 3.93TDD66 pKa = 3.35NGQGKK71 pKa = 9.76NDD73 pKa = 4.1GNNFDD78 pKa = 3.75QDD80 pKa = 3.63YY81 pKa = 10.18GNNYY85 pKa = 9.42HH86 pKa = 7.29GNDD89 pKa = 3.31KK90 pKa = 11.22DD91 pKa = 3.25NWYY94 pKa = 10.54DD95 pKa = 3.72GFWNKK100 pKa = 9.58KK101 pKa = 10.02DD102 pKa = 3.46KK103 pKa = 11.47GFDD106 pKa = 2.89KK107 pKa = 11.02CYY109 pKa = 11.01DD110 pKa = 3.57KK111 pKa = 11.25DD112 pKa = 5.25YY113 pKa = 9.8DD114 pKa = 4.31TLWNCHH120 pKa = 6.11DD121 pKa = 4.28TDD123 pKa = 4.22EE124 pKa = 5.68CCDD127 pKa = 4.32DD128 pKa = 6.01DD129 pKa = 5.25DD130 pKa = 4.06WCNDD134 pKa = 3.72EE135 pKa = 5.53YY136 pKa = 11.36CDD138 pKa = 5.31NDD140 pKa = 4.09DD141 pKa = 3.87WCDD144 pKa = 5.32DD145 pKa = 4.75DD146 pKa = 6.1DD147 pKa = 3.94EE148 pKa = 5.7CYY150 pKa = 9.95NANYY154 pKa = 9.85INNQIAINNDD164 pKa = 2.72INNVNVNTASNAGTEE179 pKa = 4.18LASGSKK185 pKa = 10.3ASQIYY190 pKa = 10.82GEE192 pKa = 4.07IQEE195 pKa = 4.29YY196 pKa = 10.38NYY198 pKa = 10.83KK199 pKa = 10.25NRR201 pKa = 11.84SANEE205 pKa = 3.74LPMQKK210 pKa = 8.83TGLPVVPALLSTLLVGSGLLYY231 pKa = 10.58RR232 pKa = 11.84KK233 pKa = 9.21LRR235 pKa = 11.84KK236 pKa = 9.27

Molecular weight: 26.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D2WDW5|A0A1D2WDW5_9EURY Uncharacterized protein OS=Methanobacterium sp. A39 OX=1860100 GN=A9507_03635 PE=4 SV=1
MM1 pKa = 7.48SRR3 pKa = 11.84NRR5 pKa = 11.84PLAKK9 pKa = 10.13KK10 pKa = 10.27LRR12 pKa = 11.84LAKK15 pKa = 10.37ANKK18 pKa = 8.91QSRR21 pKa = 11.84TVPVWVRR28 pKa = 11.84IKK30 pKa = 9.7TNRR33 pKa = 11.84KK34 pKa = 8.83VMSHH38 pKa = 5.9PKK40 pKa = 9.01MRR42 pKa = 11.84RR43 pKa = 11.84HH44 pKa = 5.38WRR46 pKa = 11.84RR47 pKa = 11.84SSLKK51 pKa = 9.74VV52 pKa = 3.13

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3065

0

3065

842557

43

2636

274.9

30.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.368 ± 0.048

1.177 ± 0.025

5.473 ± 0.04

7.076 ± 0.074

4.297 ± 0.038

6.982 ± 0.046

1.654 ± 0.019

9.393 ± 0.047

7.892 ± 0.062

9.038 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.025

5.561 ± 0.074

3.523 ± 0.03

2.357 ± 0.024

3.454 ± 0.036

6.474 ± 0.058

5.34 ± 0.059

6.74 ± 0.043

0.858 ± 0.014

3.7 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski