Amino acid dipepetide frequency for Methanobacterium sp. A39

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.306AlaAla: 5.306 ± 0.126
0.794AlaCys: 0.794 ± 0.038
3.542AlaAsp: 3.542 ± 0.078
4.134AlaGlu: 4.134 ± 0.076
2.651AlaPhe: 2.651 ± 0.057
4.929AlaGly: 4.929 ± 0.085
0.972AlaHis: 0.972 ± 0.038
5.563AlaIle: 5.563 ± 0.104
4.076AlaLys: 4.076 ± 0.088
6.403AlaLeu: 6.403 ± 0.115
1.649AlaMet: 1.649 ± 0.045
2.619AlaAsn: 2.619 ± 0.064
1.977AlaPro: 1.977 ± 0.049
1.543AlaGln: 1.543 ± 0.051
2.248AlaArg: 2.248 ± 0.055
3.989AlaSer: 3.989 ± 0.079
3.127AlaThr: 3.127 ± 0.07
5.551AlaVal: 5.551 ± 0.097
0.457AlaTrp: 0.457 ± 0.026
2.153AlaTyr: 2.153 ± 0.06
0.0AlaXaa: 0.0 ± 0.0
Cys
0.687CysAla: 0.687 ± 0.03
0.196CysCys: 0.196 ± 0.016
0.593CysAsp: 0.593 ± 0.028
0.722CysGlu: 0.722 ± 0.037
0.406CysPhe: 0.406 ± 0.023
1.347CysGly: 1.347 ± 0.062
0.233CysHis: 0.233 ± 0.017
0.948CysIle: 0.948 ± 0.038
0.822CysLys: 0.822 ± 0.032
0.841CysLeu: 0.841 ± 0.035
0.282CysMet: 0.282 ± 0.02
0.615CysAsn: 0.615 ± 0.029
0.801CysPro: 0.801 ± 0.044
0.269CysGln: 0.269 ± 0.019
0.437CysArg: 0.437 ± 0.025
0.792CysSer: 0.792 ± 0.032
0.578CysThr: 0.578 ± 0.029
0.697CysVal: 0.697 ± 0.03
0.091CysTrp: 0.091 ± 0.01
0.408CysTyr: 0.408 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.381AspAla: 3.381 ± 0.076
0.525AspCys: 0.525 ± 0.027
2.803AspAsp: 2.803 ± 0.063
4.461AspGlu: 4.461 ± 0.094
2.536AspPhe: 2.536 ± 0.063
3.226AspGly: 3.226 ± 0.072
0.879AspHis: 0.879 ± 0.034
5.938AspIle: 5.938 ± 0.091
4.179AspLys: 4.179 ± 0.079
5.293AspLeu: 5.293 ± 0.079
1.532AspMet: 1.532 ± 0.048
2.781AspAsn: 2.781 ± 0.061
2.323AspPro: 2.323 ± 0.056
1.118AspGln: 1.118 ± 0.041
1.609AspArg: 1.609 ± 0.047
2.914AspSer: 2.914 ± 0.066
2.505AspThr: 2.505 ± 0.057
4.088AspVal: 4.088 ± 0.072
0.406AspTrp: 0.406 ± 0.024
2.234AspTyr: 2.234 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
3.995GluAla: 3.995 ± 0.095
0.804GluCys: 0.804 ± 0.038
4.292GluAsp: 4.292 ± 0.089
6.211GluGlu: 6.211 ± 0.139
2.835GluPhe: 2.835 ± 0.069
4.242GluGly: 4.242 ± 0.089
1.088GluHis: 1.088 ± 0.039
7.148GluIle: 7.148 ± 0.117
6.514GluLys: 6.514 ± 0.117
6.504GluLeu: 6.504 ± 0.105
1.954GluMet: 1.954 ± 0.05
4.758GluAsn: 4.758 ± 0.082
1.759GluPro: 1.759 ± 0.049
1.404GluGln: 1.404 ± 0.042
2.688GluArg: 2.688 ± 0.073
3.942GluSer: 3.942 ± 0.07
3.271GluThr: 3.271 ± 0.064
4.338GluVal: 4.338 ± 0.085
0.522GluTrp: 0.522 ± 0.027
2.492GluTyr: 2.492 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
2.67PheAla: 2.67 ± 0.068
0.536PheCys: 0.536 ± 0.027
2.386PheAsp: 2.386 ± 0.062
2.784PheGlu: 2.784 ± 0.065
1.861PhePhe: 1.861 ± 0.06
3.143PheGly: 3.143 ± 0.066
0.668PheHis: 0.668 ± 0.028
4.033PheIle: 4.033 ± 0.091
3.5PheLys: 3.5 ± 0.072
4.171PheLeu: 4.171 ± 0.098
1.218PheMet: 1.218 ± 0.042
2.364PheAsn: 2.364 ± 0.063
1.412PhePro: 1.412 ± 0.038
0.983PheGln: 0.983 ± 0.039
1.411PheArg: 1.411 ± 0.043
2.841PheSer: 2.841 ± 0.059
2.327PheThr: 2.327 ± 0.058
2.666PheVal: 2.666 ± 0.064
0.407PheTrp: 0.407 ± 0.023
1.588PheTyr: 1.588 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
4.829GlyAla: 4.829 ± 0.094
0.898GlyCys: 0.898 ± 0.045
3.48GlyAsp: 3.48 ± 0.062
4.12GlyGlu: 4.12 ± 0.084
3.135GlyPhe: 3.135 ± 0.069
4.625GlyGly: 4.625 ± 0.107
1.26GlyHis: 1.26 ± 0.041
7.344GlyIle: 7.344 ± 0.1
5.506GlyLys: 5.506 ± 0.095
6.006GlyLeu: 6.006 ± 0.105
1.88GlyMet: 1.88 ± 0.054
3.834GlyAsn: 3.834 ± 0.094
1.804GlyPro: 1.804 ± 0.043
1.484GlyGln: 1.484 ± 0.045
2.392GlyArg: 2.392 ± 0.062
4.581GlySer: 4.581 ± 0.089
4.408GlyThr: 4.408 ± 0.104
4.72GlyVal: 4.72 ± 0.078
0.668GlyTrp: 0.668 ± 0.031
2.844GlyTyr: 2.844 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.06HisAla: 1.06 ± 0.035
0.212HisCys: 0.212 ± 0.016
0.966HisAsp: 0.966 ± 0.033
1.224HisGlu: 1.224 ± 0.041
0.739HisPhe: 0.739 ± 0.03
1.349HisGly: 1.349 ± 0.04
0.394HisHis: 0.394 ± 0.025
1.391HisIle: 1.391 ± 0.044
1.106HisLys: 1.106 ± 0.037
1.396HisLeu: 1.396 ± 0.044
0.43HisMet: 0.43 ± 0.022
0.735HisAsn: 0.735 ± 0.028
0.828HisPro: 0.828 ± 0.029
0.355HisGln: 0.355 ± 0.023
0.61HisArg: 0.61 ± 0.027
0.966HisSer: 0.966 ± 0.036
0.75HisThr: 0.75 ± 0.031
1.192HisVal: 1.192 ± 0.034
0.161HisTrp: 0.161 ± 0.016
0.671HisTyr: 0.671 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.152IleAla: 6.152 ± 0.098
1.104IleCys: 1.104 ± 0.044
4.901IleAsp: 4.901 ± 0.089
6.347IleGlu: 6.347 ± 0.103
4.134IlePhe: 4.134 ± 0.092
6.471IleGly: 6.471 ± 0.106
1.511IleHis: 1.511 ± 0.049
8.782IleIle: 8.782 ± 0.128
7.299IleLys: 7.299 ± 0.101
9.012IleLeu: 9.012 ± 0.128
2.272IleMet: 2.272 ± 0.056
5.023IleAsn: 5.023 ± 0.09
3.842IlePro: 3.842 ± 0.074
2.027IleGln: 2.027 ± 0.051
2.956IleArg: 2.956 ± 0.07
6.561IleSer: 6.561 ± 0.095
5.514IleThr: 5.514 ± 0.086
5.994IleVal: 5.994 ± 0.084
0.735IleTrp: 0.735 ± 0.032
3.296IleTyr: 3.296 ± 0.08
0.0IleXaa: 0.0 ± 0.0
Lys
4.356LysAla: 4.356 ± 0.075
0.99LysCys: 0.99 ± 0.042
4.514LysAsp: 4.514 ± 0.085
6.853LysGlu: 6.853 ± 0.129
2.951LysPhe: 2.951 ± 0.063
4.639LysGly: 4.639 ± 0.081
1.249LysHis: 1.249 ± 0.039
7.769LysIle: 7.769 ± 0.131
6.932LysLys: 6.932 ± 0.116
6.286LysLeu: 6.286 ± 0.097
2.478LysMet: 2.478 ± 0.052
5.069LysAsn: 5.069 ± 0.091
2.45LysPro: 2.45 ± 0.054
1.681LysGln: 1.681 ± 0.047
3.124LysArg: 3.124 ± 0.063
4.802LysSer: 4.802 ± 0.082
4.383LysThr: 4.383 ± 0.075
4.483LysVal: 4.483 ± 0.072
0.703LysTrp: 0.703 ± 0.033
3.208LysTyr: 3.208 ± 0.07
0.0LysXaa: 0.0 ± 0.0
Leu
5.651LeuAla: 5.651 ± 0.093
0.944LeuCys: 0.944 ± 0.035
5.05LeuAsp: 5.05 ± 0.095
6.309LeuGlu: 6.309 ± 0.118
4.006LeuPhe: 4.006 ± 0.096
6.173LeuGly: 6.173 ± 0.093
1.306LeuHis: 1.306 ± 0.041
8.579LeuIle: 8.579 ± 0.149
8.238LeuLys: 8.238 ± 0.111
7.918LeuLeu: 7.918 ± 0.148
2.33LeuMet: 2.33 ± 0.054
5.627LeuAsn: 5.627 ± 0.085
3.285LeuPro: 3.285 ± 0.063
2.215LeuGln: 2.215 ± 0.052
3.067LeuArg: 3.067 ± 0.062
6.097LeuSer: 6.097 ± 0.086
4.648LeuThr: 4.648 ± 0.084
5.525LeuVal: 5.525 ± 0.098
0.712LeuTrp: 0.712 ± 0.033
2.706LeuTyr: 2.706 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
1.954MetAla: 1.954 ± 0.047
0.261MetCys: 0.261 ± 0.021
1.765MetAsp: 1.765 ± 0.049
2.129MetGlu: 2.129 ± 0.049
0.973MetPhe: 0.973 ± 0.037
2.103MetGly: 2.103 ± 0.045
0.419MetHis: 0.419 ± 0.023
2.173MetIle: 2.173 ± 0.049
2.142MetLys: 2.142 ± 0.053
2.121MetLeu: 2.121 ± 0.055
0.677MetMet: 0.677 ± 0.035
1.449MetAsn: 1.449 ± 0.045
0.977MetPro: 0.977 ± 0.036
0.692MetGln: 0.692 ± 0.027
0.887MetArg: 0.887 ± 0.029
1.617MetSer: 1.617 ± 0.04
1.22MetThr: 1.22 ± 0.04
1.933MetVal: 1.933 ± 0.052
0.207MetTrp: 0.207 ± 0.016
0.724MetTyr: 0.724 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.425AsnAla: 3.425 ± 0.077
0.687AsnCys: 0.687 ± 0.035
2.59AsnAsp: 2.59 ± 0.055
3.519AsnGlu: 3.519 ± 0.071
2.282AsnPhe: 2.282 ± 0.058
3.898AsnGly: 3.898 ± 0.106
0.866AsnHis: 0.866 ± 0.031
5.384AsnIle: 5.384 ± 0.097
4.01AsnLys: 4.01 ± 0.085
5.006AsnLeu: 5.006 ± 0.087
1.454AsnMet: 1.454 ± 0.044
3.172AsnAsn: 3.172 ± 0.114
2.469AsnPro: 2.469 ± 0.051
1.518AsnGln: 1.518 ± 0.046
1.784AsnArg: 1.784 ± 0.049
3.948AsnSer: 3.948 ± 0.105
2.926AsnThr: 2.926 ± 0.09
3.75AsnVal: 3.75 ± 0.075
0.577AsnTrp: 0.577 ± 0.031
2.346AsnTyr: 2.346 ± 0.065
0.0AsnXaa: 0.0 ± 0.0
Pro
2.124ProAla: 2.124 ± 0.049
0.405ProCys: 0.405 ± 0.02
2.248ProAsp: 2.248 ± 0.054
3.29ProGlu: 3.29 ± 0.075
1.656ProPhe: 1.656 ± 0.046
2.287ProGly: 2.287 ± 0.058
0.722ProHis: 0.722 ± 0.032
2.587ProIle: 2.587 ± 0.052
2.437ProLys: 2.437 ± 0.052
3.273ProLeu: 3.273 ± 0.072
0.789ProMet: 0.789 ± 0.029
1.498ProAsn: 1.498 ± 0.048
1.037ProPro: 1.037 ± 0.032
1.08ProGln: 1.08 ± 0.036
1.091ProArg: 1.091 ± 0.036
2.178ProSer: 2.178 ± 0.054
1.796ProThr: 1.796 ± 0.047
3.054ProVal: 3.054 ± 0.073
0.312ProTrp: 0.312 ± 0.023
1.366ProTyr: 1.366 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
1.347GlnAla: 1.347 ± 0.039
0.239GlnCys: 0.239 ± 0.021
1.253GlnAsp: 1.253 ± 0.043
1.558GlnGlu: 1.558 ± 0.046
0.977GlnPhe: 0.977 ± 0.039
1.379GlnGly: 1.379 ± 0.045
0.361GlnHis: 0.361 ± 0.02
2.373GlnIle: 2.373 ± 0.055
2.167GlnLys: 2.167 ± 0.055
2.069GlnLeu: 2.069 ± 0.052
0.728GlnMet: 0.728 ± 0.029
1.594GlnAsn: 1.594 ± 0.051
0.648GlnPro: 0.648 ± 0.029
0.69GlnGln: 0.69 ± 0.033
0.953GlnArg: 0.953 ± 0.039
1.462GlnSer: 1.462 ± 0.048
1.297GlnThr: 1.297 ± 0.04
1.391GlnVal: 1.391 ± 0.038
0.198GlnTrp: 0.198 ± 0.016
0.882GlnTyr: 0.882 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
2.138ArgAla: 2.138 ± 0.05
0.395ArgCys: 0.395 ± 0.025
1.944ArgAsp: 1.944 ± 0.049
2.746ArgGlu: 2.746 ± 0.061
1.494ArgPhe: 1.494 ± 0.046
2.314ArgGly: 2.314 ± 0.062
0.593ArgHis: 0.593 ± 0.029
3.158ArgIle: 3.158 ± 0.067
3.03ArgLys: 3.03 ± 0.07
2.806ArgLeu: 2.806 ± 0.054
0.984ArgMet: 0.984 ± 0.033
1.793ArgAsn: 1.793 ± 0.049
1.087ArgPro: 1.087 ± 0.039
0.787ArgGln: 0.787 ± 0.032
1.614ArgArg: 1.614 ± 0.051
2.008ArgSer: 2.008 ± 0.05
1.675ArgThr: 1.675 ± 0.045
2.27ArgVal: 2.27 ± 0.059
0.294ArgTrp: 0.294 ± 0.019
1.41ArgTyr: 1.41 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
3.88SerAla: 3.88 ± 0.074
0.705SerCys: 0.705 ± 0.032
3.254SerAsp: 3.254 ± 0.075
3.939SerGlu: 3.939 ± 0.075
2.91SerPhe: 2.91 ± 0.07
5.133SerGly: 5.133 ± 0.114
1.095SerHis: 1.095 ± 0.037
5.729SerIle: 5.729 ± 0.079
5.105SerLys: 5.105 ± 0.08
5.778SerLeu: 5.778 ± 0.11
1.701SerMet: 1.701 ± 0.044
3.623SerAsn: 3.623 ± 0.103
2.21SerPro: 2.21 ± 0.054
1.776SerGln: 1.776 ± 0.049
2.305SerArg: 2.305 ± 0.059
4.816SerSer: 4.816 ± 0.113
3.75SerThr: 3.75 ± 0.1
4.021SerVal: 4.021 ± 0.069
0.589SerTrp: 0.589 ± 0.026
2.424SerTyr: 2.424 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
3.779ThrAla: 3.779 ± 0.09
0.682ThrCys: 0.682 ± 0.031
2.806ThrAsp: 2.806 ± 0.059
3.215ThrGlu: 3.215 ± 0.067
2.284ThrPhe: 2.284 ± 0.057
4.667ThrGly: 4.667 ± 0.088
0.954ThrHis: 0.954 ± 0.036
4.688ThrIle: 4.688 ± 0.104
3.237ThrLys: 3.237 ± 0.062
4.848ThrLeu: 4.848 ± 0.093
1.183ThrMet: 1.183 ± 0.04
2.53ThrAsn: 2.53 ± 0.071
2.12ThrPro: 2.12 ± 0.052
1.289ThrGln: 1.289 ± 0.043
1.74ThrArg: 1.74 ± 0.046
3.798ThrSer: 3.798 ± 0.092
3.009ThrThr: 3.009 ± 0.097
4.239ThrVal: 4.239 ± 0.08
0.446ThrTrp: 0.446 ± 0.024
1.886ThrTyr: 1.886 ± 0.053
0.0ThrXaa: 0.0 ± 0.0
Val
4.334ValAla: 4.334 ± 0.081
0.796ValCys: 0.796 ± 0.035
3.832ValAsp: 3.832 ± 0.076
4.493ValGlu: 4.493 ± 0.094
2.929ValPhe: 2.929 ± 0.063
4.421ValGly: 4.421 ± 0.072
1.148ValHis: 1.148 ± 0.034
6.365ValIle: 6.365 ± 0.097
5.32ValLys: 5.32 ± 0.091
6.362ValLeu: 6.362 ± 0.084
1.652ValMet: 1.652 ± 0.047
3.607ValAsn: 3.607 ± 0.072
2.564ValPro: 2.564 ± 0.05
1.58ValGln: 1.58 ± 0.037
2.047ValArg: 2.047 ± 0.052
4.454ValSer: 4.454 ± 0.072
3.872ValThr: 3.872 ± 0.079
4.725ValVal: 4.725 ± 0.089
0.535ValTrp: 0.535 ± 0.027
2.361ValTyr: 2.361 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.482TrpAla: 0.482 ± 0.024
0.1TrpCys: 0.1 ± 0.011
0.496TrpAsp: 0.496 ± 0.022
0.491TrpGlu: 0.491 ± 0.024
0.381TrpPhe: 0.381 ± 0.023
0.578TrpGly: 0.578 ± 0.029
0.144TrpHis: 0.144 ± 0.013
0.832TrpIle: 0.832 ± 0.032
0.703TrpLys: 0.703 ± 0.035
0.793TrpLeu: 0.793 ± 0.033
0.254TrpMet: 0.254 ± 0.017
0.601TrpAsn: 0.601 ± 0.027
0.212TrpPro: 0.212 ± 0.017
0.215TrpGln: 0.215 ± 0.017
0.318TrpArg: 0.318 ± 0.022
0.47TrpSer: 0.47 ± 0.026
0.452TrpThr: 0.452 ± 0.027
0.51TrpVal: 0.51 ± 0.024
0.17TrpTrp: 0.17 ± 0.017
0.383TrpTyr: 0.383 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.133TyrAla: 2.133 ± 0.06
0.494TyrCys: 0.494 ± 0.025
2.018TyrAsp: 2.018 ± 0.054
2.214TyrGlu: 2.214 ± 0.057
1.83TyrPhe: 1.83 ± 0.055
2.892TyrGly: 2.892 ± 0.068
0.673TyrHis: 0.673 ± 0.025
3.148TyrIle: 3.148 ± 0.063
2.596TyrLys: 2.596 ± 0.059
3.499TyrLeu: 3.499 ± 0.066
0.976TyrMet: 0.976 ± 0.035
2.319TyrAsn: 2.319 ± 0.063
1.424TyrPro: 1.424 ± 0.041
0.862TyrGln: 0.862 ± 0.033
1.262TyrArg: 1.262 ± 0.039
2.508TyrSer: 2.508 ± 0.066
1.892TyrThr: 1.892 ± 0.059
2.25TyrVal: 2.25 ± 0.056
0.394TyrTrp: 0.394 ± 0.023
1.622TyrTyr: 1.622 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3065 proteins (842557 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski