Podoviridae sp. ctrTa16
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W6T6|A0A5Q2W6T6_9CAUD Uncharacterized protein OS=Podoviridae sp. ctrTa16 OX=2656715 PE=4 SV=1
MM1 pKa = 7.36 ATSQTYY7 pKa = 9.13 YY8 pKa = 11.29 LNGASLGSSTSIFINPEE25 pKa = 3.86 LTILGADD32 pKa = 4.03 GFYY35 pKa = 10.66 SDD37 pKa = 4.1 GVIVRR42 pKa = 11.84 EE43 pKa = 3.9 QSGGILLPQQPCPSCATQCGEE64 pKa = 4.41 SVITSFDD71 pKa = 3.9 SNGVYY76 pKa = 9.46 MLPIDD81 pKa = 4.23 AGSSVGAIIIRR92 pKa = 11.84 FNPFSIPIGIQAVFNSITYY111 pKa = 9.7 NKK113 pKa = 9.69 LVSATYY119 pKa = 10.23 GVLEE123 pKa = 4.44 GSPGLLTYY131 pKa = 10.55 VGNTANDD138 pKa = 3.88 CGLVAGSPHH147 pKa = 6.17 TLDD150 pKa = 4.99 DD151 pKa = 4.1 FVFNGTSFVPQATQSIVSITAPEE174 pKa = 4.12 VQTTVASPGEE184 pKa = 4.27 CVMIIPKK191 pKa = 8.18 TTASPSAVDD200 pKa = 3.33 VTIAAACASAVFNVKK215 pKa = 9.82 IEE217 pKa = 4.25 CPAFLSSFEE226 pKa = 3.95 ISEE229 pKa = 4.55 ANVDD233 pKa = 3.78 APAACLSSVSTDD245 pKa = 2.88 AYY247 pKa = 10.34 VAFINGSGTVFGLYY261 pKa = 10.5 DD262 pKa = 4.99 CIFSDD267 pKa = 3.81 ATGEE271 pKa = 4.09 FPLPDD276 pKa = 3.99 GYY278 pKa = 11.76 YY279 pKa = 8.08 KK280 pKa = 10.75 TNLISVPPGYY290 pKa = 11.09 DD291 pKa = 2.53 WMRR294 pKa = 11.84 LQNGVIIEE302 pKa = 4.49 FGNCPP307 pKa = 3.36
Molecular weight: 31.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.892
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.554
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.884
Patrickios 0.006
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A5Q2W8T6|A0A5Q2W8T6_9CAUD Structural protein OS=Podoviridae sp. ctrTa16 OX=2656715 PE=4 SV=1
MM1 pKa = 7.75 ADD3 pKa = 3.37 KK4 pKa = 11.25 SKK6 pKa = 10.16 MKK8 pKa = 10.31 CNRR11 pKa = 11.84 PVPSDD16 pKa = 3.48 RR17 pKa = 11.84 PGKK20 pKa = 10.7 KK21 pKa = 9.6 MMVKK25 pKa = 9.51 ACSGGVEE32 pKa = 4.13 KK33 pKa = 10.67 LLHH36 pKa = 6.54 FGAEE40 pKa = 4.91 GYY42 pKa = 9.1 GHH44 pKa = 6.93 NYY46 pKa = 8.7 SAAARR51 pKa = 11.84 KK52 pKa = 9.48 SFRR55 pKa = 11.84 ARR57 pKa = 11.84 HH58 pKa = 5.08 GCEE61 pKa = 3.42 SATNKK66 pKa = 7.79 LTPKK70 pKa = 9.85 HH71 pKa = 5.48 WACSYY76 pKa = 10.55 LWAGKK81 pKa = 10.33 GGSTKK86 pKa = 10.71 SNPKK90 pKa = 9.06 NRR92 pKa = 11.84 KK93 pKa = 8.47 GKK95 pKa = 9.62 YY96 pKa = 8.81
Molecular weight: 10.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.197
IPC2_protein 9.545
IPC_protein 9.516
Toseland 10.438
ProMoST 9.97
Dawson 10.54
Bjellqvist 10.16
Wikipedia 10.657
Rodwell 11.228
Grimsley 10.584
Solomon 10.57
Lehninger 10.555
Nozaki 10.438
DTASelect 10.145
Thurlkill 10.423
EMBOSS 10.804
Sillero 10.452
Patrickios 10.95
IPC_peptide 10.57
IPC2_peptide 8.916
IPC2.peptide.svr19 8.483
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
70
0
70
18610
34
2943
265.9
30.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.814 ± 0.377
0.951 ± 0.206
5.986 ± 0.143
7.474 ± 0.442
4.546 ± 0.175
6.373 ± 0.284
1.3 ± 0.173
7.093 ± 0.192
7.942 ± 0.54
7.41 ± 0.209
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.676 ± 0.162
5.97 ± 0.188
3.385 ± 0.199
3.321 ± 0.204
4.39 ± 0.213
7.227 ± 0.265
5.61 ± 0.339
6.179 ± 0.281
0.973 ± 0.105
4.379 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here