Amino acid dipepetide frequency for Leptonema illini DSM 21528

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.621AlaAla: 8.621 ± 0.114
0.852AlaCys: 0.852 ± 0.029
5.181AlaAsp: 5.181 ± 0.07
5.882AlaGlu: 5.882 ± 0.067
3.974AlaPhe: 3.974 ± 0.06
7.516AlaGly: 7.516 ± 0.1
1.588AlaHis: 1.588 ± 0.039
4.853AlaIle: 4.853 ± 0.072
3.312AlaLys: 3.312 ± 0.055
9.931AlaLeu: 9.931 ± 0.099
2.36AlaMet: 2.36 ± 0.044
2.202AlaAsn: 2.202 ± 0.044
3.214AlaPro: 3.214 ± 0.053
2.431AlaGln: 2.431 ± 0.045
5.742AlaArg: 5.742 ± 0.065
5.604AlaSer: 5.604 ± 0.065
4.0AlaThr: 4.0 ± 0.064
6.409AlaVal: 6.409 ± 0.086
0.822AlaTrp: 0.822 ± 0.028
2.368AlaTyr: 2.368 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.662CysAla: 0.662 ± 0.024
0.089CysCys: 0.089 ± 0.009
0.459CysAsp: 0.459 ± 0.018
0.532CysGlu: 0.532 ± 0.021
0.374CysPhe: 0.374 ± 0.018
0.735CysGly: 0.735 ± 0.025
0.237CysHis: 0.237 ± 0.014
0.526CysIle: 0.526 ± 0.02
0.34CysLys: 0.34 ± 0.016
0.788CysLeu: 0.788 ± 0.027
0.221CysMet: 0.221 ± 0.013
0.286CysAsn: 0.286 ± 0.016
0.414CysPro: 0.414 ± 0.018
0.212CysGln: 0.212 ± 0.013
0.622CysArg: 0.622 ± 0.023
0.703CysSer: 0.703 ± 0.025
0.458CysThr: 0.458 ± 0.022
0.549CysVal: 0.549 ± 0.022
0.072CysTrp: 0.072 ± 0.007
0.302CysTyr: 0.302 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.923AspAla: 4.923 ± 0.067
0.45AspCys: 0.45 ± 0.021
3.031AspAsp: 3.031 ± 0.06
4.137AspGlu: 4.137 ± 0.064
2.727AspPhe: 2.727 ± 0.045
4.285AspGly: 4.285 ± 0.058
1.124AspHis: 1.124 ± 0.027
3.054AspIle: 3.054 ± 0.059
1.416AspLys: 1.416 ± 0.036
6.443AspLeu: 6.443 ± 0.085
1.23AspMet: 1.23 ± 0.034
1.244AspAsn: 1.244 ± 0.033
2.987AspPro: 2.987 ± 0.052
1.643AspGln: 1.643 ± 0.038
5.188AspArg: 5.188 ± 0.07
3.112AspSer: 3.112 ± 0.053
2.492AspThr: 2.492 ± 0.042
3.501AspVal: 3.501 ± 0.05
0.714AspTrp: 0.714 ± 0.024
1.762AspTyr: 1.762 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
6.146GluAla: 6.146 ± 0.082
0.434GluCys: 0.434 ± 0.018
3.144GluAsp: 3.144 ± 0.053
5.444GluGlu: 5.444 ± 0.094
2.525GluPhe: 2.525 ± 0.047
4.38GluGly: 4.38 ± 0.063
1.441GluHis: 1.441 ± 0.036
4.557GluIle: 4.557 ± 0.065
4.754GluLys: 4.754 ± 0.068
6.605GluLeu: 6.605 ± 0.081
1.85GluMet: 1.85 ± 0.04
2.421GluAsn: 2.421 ± 0.041
2.316GluPro: 2.316 ± 0.042
2.928GluGln: 2.928 ± 0.058
5.553GluArg: 5.553 ± 0.068
4.356GluSer: 4.356 ± 0.072
3.291GluThr: 3.291 ± 0.046
3.389GluVal: 3.389 ± 0.05
0.756GluTrp: 0.756 ± 0.022
1.954GluTyr: 1.954 ± 0.039
0.0GluXaa: 0.0 ± 0.0
Phe
4.007PheAla: 4.007 ± 0.058
0.439PheCys: 0.439 ± 0.02
2.588PheAsp: 2.588 ± 0.052
2.899PheGlu: 2.899 ± 0.045
2.866PhePhe: 2.866 ± 0.063
3.078PheGly: 3.078 ± 0.045
1.106PheHis: 1.106 ± 0.031
2.345PheIle: 2.345 ± 0.047
1.585PheLys: 1.585 ± 0.035
5.35PheLeu: 5.35 ± 0.075
0.916PheMet: 0.916 ± 0.026
1.28PheAsn: 1.28 ± 0.031
2.008PhePro: 2.008 ± 0.04
1.689PheGln: 1.689 ± 0.03
3.311PheArg: 3.311 ± 0.055
3.242PheSer: 3.242 ± 0.047
2.347PheThr: 2.347 ± 0.046
3.054PheVal: 3.054 ± 0.05
0.596PheTrp: 0.596 ± 0.023
1.791PheTyr: 1.791 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
5.65GlyAla: 5.65 ± 0.072
0.732GlyCys: 0.732 ± 0.029
3.759GlyAsp: 3.759 ± 0.059
4.413GlyGlu: 4.413 ± 0.07
3.646GlyPhe: 3.646 ± 0.059
5.201GlyGly: 5.201 ± 0.077
1.516GlyHis: 1.516 ± 0.036
4.857GlyIle: 4.857 ± 0.055
3.456GlyLys: 3.456 ± 0.064
7.478GlyLeu: 7.478 ± 0.084
2.179GlyMet: 2.179 ± 0.041
2.286GlyAsn: 2.286 ± 0.049
2.58GlyPro: 2.58 ± 0.05
2.211GlyGln: 2.211 ± 0.044
5.403GlyArg: 5.403 ± 0.074
4.713GlySer: 4.713 ± 0.057
3.727GlyThr: 3.727 ± 0.06
4.722GlyVal: 4.722 ± 0.059
1.009GlyTrp: 1.009 ± 0.027
2.504GlyTyr: 2.504 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
1.747HisAla: 1.747 ± 0.039
0.244HisCys: 0.244 ± 0.012
1.067HisAsp: 1.067 ± 0.026
1.231HisGlu: 1.231 ± 0.029
1.035HisPhe: 1.035 ± 0.03
1.569HisGly: 1.569 ± 0.034
0.576HisHis: 0.576 ± 0.023
1.25HisIle: 1.25 ± 0.03
0.655HisLys: 0.655 ± 0.026
2.388HisLeu: 2.388 ± 0.041
0.429HisMet: 0.429 ± 0.019
0.583HisAsn: 0.583 ± 0.021
1.439HisPro: 1.439 ± 0.036
0.599HisGln: 0.599 ± 0.023
1.848HisArg: 1.848 ± 0.042
1.349HisSer: 1.349 ± 0.031
1.034HisThr: 1.034 ± 0.028
1.188HisVal: 1.188 ± 0.031
0.272HisTrp: 0.272 ± 0.015
0.774HisTyr: 0.774 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.366IleAla: 5.366 ± 0.072
0.55IleCys: 0.55 ± 0.02
3.881IleAsp: 3.881 ± 0.056
4.893IleGlu: 4.893 ± 0.06
2.604IlePhe: 2.604 ± 0.044
4.314IleGly: 4.314 ± 0.06
1.395IleHis: 1.395 ± 0.036
3.048IleIle: 3.048 ± 0.056
2.207IleLys: 2.207 ± 0.043
6.503IleLeu: 6.503 ± 0.084
1.077IleMet: 1.077 ± 0.029
1.743IleAsn: 1.743 ± 0.041
3.037IlePro: 3.037 ± 0.051
2.209IleGln: 2.209 ± 0.045
4.543IleArg: 4.543 ± 0.068
3.733IleSer: 3.733 ± 0.05
2.948IleThr: 2.948 ± 0.051
4.463IleVal: 4.463 ± 0.057
0.587IleTrp: 0.587 ± 0.021
1.784IleTyr: 1.784 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
4.143LysAla: 4.143 ± 0.064
0.224LysCys: 0.224 ± 0.012
2.541LysAsp: 2.541 ± 0.047
3.837LysGlu: 3.837 ± 0.068
1.209LysPhe: 1.209 ± 0.033
3.249LysGly: 3.249 ± 0.058
0.835LysHis: 0.835 ± 0.023
2.744LysIle: 2.744 ± 0.057
3.677LysLys: 3.677 ± 0.067
3.535LysLeu: 3.535 ± 0.054
1.153LysMet: 1.153 ± 0.028
1.874LysAsn: 1.874 ± 0.04
1.91LysPro: 1.91 ± 0.041
1.778LysGln: 1.778 ± 0.034
3.118LysArg: 3.118 ± 0.049
2.804LysSer: 2.804 ± 0.049
2.538LysThr: 2.538 ± 0.049
2.215LysVal: 2.215 ± 0.047
0.405LysTrp: 0.405 ± 0.015
1.159LysTyr: 1.159 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
9.059LeuAla: 9.059 ± 0.084
1.006LeuCys: 1.006 ± 0.027
5.454LeuAsp: 5.454 ± 0.062
6.373LeuGlu: 6.373 ± 0.083
5.672LeuPhe: 5.672 ± 0.094
6.032LeuGly: 6.032 ± 0.075
2.521LeuHis: 2.521 ± 0.047
6.322LeuIle: 6.322 ± 0.08
5.539LeuLys: 5.539 ± 0.078
12.441LeuLeu: 12.441 ± 0.167
2.35LeuMet: 2.35 ± 0.044
3.571LeuAsn: 3.571 ± 0.053
5.482LeuPro: 5.482 ± 0.069
3.932LeuGln: 3.932 ± 0.059
7.4LeuArg: 7.4 ± 0.084
7.859LeuSer: 7.859 ± 0.093
5.297LeuThr: 5.297 ± 0.064
5.926LeuVal: 5.926 ± 0.069
1.16LeuTrp: 1.16 ± 0.033
3.434LeuTyr: 3.434 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.26MetAla: 2.26 ± 0.044
0.124MetCys: 0.124 ± 0.009
1.219MetAsp: 1.219 ± 0.029
1.364MetGlu: 1.364 ± 0.033
0.741MetPhe: 0.741 ± 0.023
1.493MetGly: 1.493 ± 0.031
0.542MetHis: 0.542 ± 0.022
1.652MetIle: 1.652 ± 0.037
1.83MetLys: 1.83 ± 0.035
2.59MetLeu: 2.59 ± 0.043
0.639MetMet: 0.639 ± 0.022
1.165MetAsn: 1.165 ± 0.029
1.234MetPro: 1.234 ± 0.033
1.187MetGln: 1.187 ± 0.03
1.736MetArg: 1.736 ± 0.033
1.624MetSer: 1.624 ± 0.034
1.371MetThr: 1.371 ± 0.028
1.277MetVal: 1.277 ± 0.037
0.201MetTrp: 0.201 ± 0.016
0.443MetTyr: 0.443 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.636AsnAla: 2.636 ± 0.046
0.273AsnCys: 0.273 ± 0.017
1.734AsnAsp: 1.734 ± 0.036
2.007AsnGlu: 2.007 ± 0.044
1.184AsnPhe: 1.184 ± 0.031
2.523AsnGly: 2.523 ± 0.049
0.68AsnHis: 0.68 ± 0.022
1.802AsnIle: 1.802 ± 0.035
0.971AsnLys: 0.971 ± 0.03
3.255AsnLeu: 3.255 ± 0.054
0.654AsnMet: 0.654 ± 0.023
0.879AsnAsn: 0.879 ± 0.03
1.999AsnPro: 1.999 ± 0.043
1.017AsnGln: 1.017 ± 0.03
2.847AsnArg: 2.847 ± 0.044
1.758AsnSer: 1.758 ± 0.041
1.467AsnThr: 1.467 ± 0.038
1.923AsnVal: 1.923 ± 0.039
0.406AsnTrp: 0.406 ± 0.018
0.978AsnTyr: 0.978 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
4.653ProAla: 4.653 ± 0.069
0.292ProCys: 0.292 ± 0.015
3.536ProAsp: 3.536 ± 0.053
4.065ProGlu: 4.065 ± 0.06
2.315ProPhe: 2.315 ± 0.042
3.949ProGly: 3.949 ± 0.061
0.891ProHis: 0.891 ± 0.031
2.125ProIle: 2.125 ± 0.039
1.174ProLys: 1.174 ± 0.031
4.466ProLeu: 4.466 ± 0.072
0.959ProMet: 0.959 ± 0.025
0.986ProAsn: 0.986 ± 0.025
1.825ProPro: 1.825 ± 0.041
1.189ProGln: 1.189 ± 0.028
1.806ProArg: 1.806 ± 0.042
2.728ProSer: 2.728 ± 0.048
1.98ProThr: 1.98 ± 0.044
4.209ProVal: 4.209 ± 0.056
0.414ProTrp: 0.414 ± 0.02
1.423ProTyr: 1.423 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
3.297GlnAla: 3.297 ± 0.057
0.313GlnCys: 0.313 ± 0.016
1.407GlnAsp: 1.407 ± 0.031
2.159GlnGlu: 2.159 ± 0.045
1.357GlnPhe: 1.357 ± 0.033
2.18GlnGly: 2.18 ± 0.042
0.584GlnHis: 0.584 ± 0.025
2.506GlnIle: 2.506 ± 0.041
2.122GlnLys: 2.122 ± 0.045
3.033GlnLeu: 3.033 ± 0.051
1.009GlnMet: 1.009 ± 0.026
1.288GlnAsn: 1.288 ± 0.031
1.42GlnPro: 1.42 ± 0.036
1.141GlnGln: 1.141 ± 0.034
2.467GlnArg: 2.467 ± 0.048
2.518GlnSer: 2.518 ± 0.053
1.908GlnThr: 1.908 ± 0.039
1.704GlnVal: 1.704 ± 0.036
0.431GlnTrp: 0.431 ± 0.018
1.043GlnTyr: 1.043 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
4.902ArgAla: 4.902 ± 0.06
0.57ArgCys: 0.57 ± 0.022
3.551ArgAsp: 3.551 ± 0.053
4.929ArgGlu: 4.929 ± 0.072
3.764ArgPhe: 3.764 ± 0.059
3.781ArgGly: 3.781 ± 0.059
1.616ArgHis: 1.616 ± 0.035
5.493ArgIle: 5.493 ± 0.066
3.41ArgLys: 3.41 ± 0.066
8.076ArgLeu: 8.076 ± 0.096
2.253ArgMet: 2.253 ± 0.042
2.505ArgAsn: 2.505 ± 0.043
3.309ArgPro: 3.309 ± 0.048
2.525ArgGln: 2.525 ± 0.045
5.715ArgArg: 5.715 ± 0.09
5.224ArgSer: 5.224 ± 0.066
3.422ArgThr: 3.422 ± 0.053
3.811ArgVal: 3.811 ± 0.044
0.94ArgTrp: 0.94 ± 0.027
2.427ArgTyr: 2.427 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.545SerAla: 5.545 ± 0.066
0.694SerCys: 0.694 ± 0.027
3.707SerAsp: 3.707 ± 0.057
4.114SerGlu: 4.114 ± 0.052
3.208SerPhe: 3.208 ± 0.059
5.859SerGly: 5.859 ± 0.075
1.268SerHis: 1.268 ± 0.029
4.076SerIle: 4.076 ± 0.057
2.279SerLys: 2.279 ± 0.039
7.326SerLeu: 7.326 ± 0.074
1.677SerMet: 1.677 ± 0.038
1.61SerAsn: 1.61 ± 0.032
2.789SerPro: 2.789 ± 0.054
1.871SerGln: 1.871 ± 0.043
4.492SerArg: 4.492 ± 0.065
4.448SerSer: 4.448 ± 0.084
3.141SerThr: 3.141 ± 0.052
4.786SerVal: 4.786 ± 0.062
0.682SerTrp: 0.682 ± 0.029
2.102SerTyr: 2.102 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
4.717ThrAla: 4.717 ± 0.072
0.34ThrCys: 0.34 ± 0.017
3.016ThrAsp: 3.016 ± 0.054
3.377ThrGlu: 3.377 ± 0.058
1.978ThrPhe: 1.978 ± 0.036
5.205ThrGly: 5.205 ± 0.075
0.909ThrHis: 0.909 ± 0.028
3.144ThrIle: 3.144 ± 0.053
1.666ThrLys: 1.666 ± 0.035
5.108ThrLeu: 5.108 ± 0.054
1.222ThrMet: 1.222 ± 0.024
1.314ThrAsn: 1.314 ± 0.034
2.409ThrPro: 2.409 ± 0.046
1.362ThrGln: 1.362 ± 0.032
2.419ThrArg: 2.419 ± 0.047
2.851ThrSer: 2.851 ± 0.06
2.63ThrThr: 2.63 ± 0.061
3.914ThrVal: 3.914 ± 0.059
0.435ThrTrp: 0.435 ± 0.018
1.393ThrTyr: 1.393 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
5.359ValAla: 5.359 ± 0.075
0.558ValCys: 0.558 ± 0.022
3.822ValAsp: 3.822 ± 0.055
4.106ValGlu: 4.106 ± 0.055
3.293ValPhe: 3.293 ± 0.055
3.796ValGly: 3.796 ± 0.063
1.441ValHis: 1.441 ± 0.035
3.987ValIle: 3.987 ± 0.056
2.763ValLys: 2.763 ± 0.056
6.937ValLeu: 6.937 ± 0.088
1.461ValMet: 1.461 ± 0.034
2.08ValAsn: 2.08 ± 0.04
2.845ValPro: 2.845 ± 0.047
2.415ValGln: 2.415 ± 0.041
4.424ValArg: 4.424 ± 0.071
4.167ValSer: 4.167 ± 0.058
3.176ValThr: 3.176 ± 0.055
4.632ValVal: 4.632 ± 0.066
0.661ValTrp: 0.661 ± 0.024
2.114ValTyr: 2.114 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
0.716TrpAla: 0.716 ± 0.022
0.085TrpCys: 0.085 ± 0.008
0.507TrpAsp: 0.507 ± 0.019
0.483TrpGlu: 0.483 ± 0.021
0.479TrpPhe: 0.479 ± 0.02
0.622TrpGly: 0.622 ± 0.023
0.258TrpHis: 0.258 ± 0.015
0.925TrpIle: 0.925 ± 0.032
0.783TrpLys: 0.783 ± 0.023
1.139TrpLeu: 1.139 ± 0.033
0.377TrpMet: 0.377 ± 0.018
0.639TrpAsn: 0.639 ± 0.02
0.451TrpPro: 0.451 ± 0.02
0.527TrpGln: 0.527 ± 0.018
0.657TrpArg: 0.657 ± 0.027
0.739TrpSer: 0.739 ± 0.026
0.633TrpThr: 0.633 ± 0.023
0.616TrpVal: 0.616 ± 0.021
0.16TrpTrp: 0.16 ± 0.014
0.325TrpTyr: 0.325 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.454TyrAla: 2.454 ± 0.047
0.315TyrCys: 0.315 ± 0.016
1.857TyrAsp: 1.857 ± 0.044
1.998TyrGlu: 1.998 ± 0.039
1.548TyrPhe: 1.548 ± 0.041
2.271TyrGly: 2.271 ± 0.045
0.752TyrHis: 0.752 ± 0.028
1.616TyrIle: 1.616 ± 0.033
1.006TyrLys: 1.006 ± 0.029
3.439TyrLeu: 3.439 ± 0.053
0.678TyrMet: 0.678 ± 0.02
1.034TyrAsn: 1.034 ± 0.029
1.318TyrPro: 1.318 ± 0.032
1.021TyrGln: 1.021 ± 0.033
2.93TyrArg: 2.93 ± 0.052
2.145TyrSer: 2.145 ± 0.053
1.549TyrThr: 1.549 ± 0.033
1.75TyrVal: 1.75 ± 0.035
0.4TyrTrp: 0.4 ± 0.018
1.252TyrTyr: 1.252 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4129 proteins (1346542 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski