Trypanosoma rangeli SC58
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7365 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A061J7B2|A0A061J7B2_TRYRA Ubiquitinyl hydrolase 1 OS=Trypanosoma rangeli SC58 OX=429131 GN=TRSC58_02040 PE=4 SV=1
MM1 pKa = 7.96 DD2 pKa = 5.76 GDD4 pKa = 4.09 FLLEE8 pKa = 3.91 RR9 pKa = 11.84 LDD11 pKa = 4.12 AFFSEE16 pKa = 4.88 GANTDD21 pKa = 3.61 AIGNFISEE29 pKa = 4.55 EE30 pKa = 3.96 QGVMQLLGHH39 pKa = 5.73 STDD42 pKa = 3.72 TQEE45 pKa = 4.88 SLQLYY50 pKa = 10.68 ALFKK54 pKa = 10.61 KK55 pKa = 10.04 YY56 pKa = 10.61 AAVVDD61 pKa = 4.41 ALLHH65 pKa = 5.18 MFVARR70 pKa = 11.84 EE71 pKa = 4.1 TEE73 pKa = 4.13 AGCPIDD79 pKa = 5.58 LEE81 pKa = 4.16 QLAAAVMKK89 pKa = 10.02 EE90 pKa = 3.24 WRR92 pKa = 11.84 QEE94 pKa = 3.54 HH95 pKa = 7.23 DD96 pKa = 3.44 YY97 pKa = 11.38 CRR99 pKa = 11.84 YY100 pKa = 10.35 LCTAYY105 pKa = 10.77 VAGALDD111 pKa = 3.91 FEE113 pKa = 4.99 SFKK116 pKa = 11.16 QLVADD121 pKa = 4.14 VNAMTTYY128 pKa = 10.46 PVGAEE133 pKa = 4.17 LSDD136 pKa = 3.56 EE137 pKa = 4.61 DD138 pKa = 4.52 SVSEE142 pKa = 3.97 ATPEE146 pKa = 4.12 EE147 pKa = 4.49 EE148 pKa = 5.47 DD149 pKa = 3.51 SS150 pKa = 3.7
Molecular weight: 16.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.787
IPC2_protein 3.973
IPC_protein 3.923
Toseland 3.732
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.783
Rodwell 3.757
Grimsley 3.643
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.164
Thurlkill 3.77
EMBOSS 3.795
Sillero 4.037
Patrickios 1.888
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|A0A061J7A0|A0A061J7A0_TRYRA Trimethylguanosine synthase OS=Trypanosoma rangeli SC58 OX=429131 GN=TRSC58_01512 PE=4 SV=1
MM1 pKa = 7.86 ISIKK5 pKa = 9.68 TGKK8 pKa = 9.75 KK9 pKa = 9.07 PRR11 pKa = 11.84 AKK13 pKa = 9.99 KK14 pKa = 10.22 VKK16 pKa = 9.42 NLKK19 pKa = 10.42 GPGGRR24 pKa = 11.84 GAAKK28 pKa = 10.21 GALQQRR34 pKa = 11.84 GARR37 pKa = 11.84 GVPGQKK43 pKa = 9.99 GAAQRR48 pKa = 11.84 GGAQKK53 pKa = 11.18 GNTRR57 pKa = 11.84 RR58 pKa = 11.84 PQQQQQKK65 pKa = 9.02 QRR67 pKa = 11.84 PQQKK71 pKa = 9.4 QSQQRR76 pKa = 11.84 GARR79 pKa = 11.84 PSRR82 pKa = 11.84 NEE84 pKa = 3.4 EE85 pKa = 3.53 GGRR88 pKa = 11.84 QQRR91 pKa = 11.84 GGNPASQARR100 pKa = 11.84 QQQGRR105 pKa = 11.84 NAPRR109 pKa = 11.84 PQKK112 pKa = 10.91 AA113 pKa = 2.92
Molecular weight: 12.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.421
IPC2_protein 10.906
IPC_protein 12.471
Toseland 12.647
ProMoST 13.144
Dawson 12.647
Bjellqvist 12.647
Wikipedia 13.13
Rodwell 12.442
Grimsley 12.691
Solomon 13.144
Lehninger 13.042
Nozaki 12.647
DTASelect 12.647
Thurlkill 12.647
EMBOSS 13.144
Sillero 12.647
Patrickios 12.149
IPC_peptide 13.144
IPC2_peptide 12.135
IPC2.peptide.svr19 9.069
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7365
0
7365
3404951
51
4449
462.3
51.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.237 ± 0.031
2.125 ± 0.013
4.785 ± 0.016
6.849 ± 0.029
3.826 ± 0.017
6.47 ± 0.019
2.675 ± 0.012
3.633 ± 0.019
4.11 ± 0.024
10.109 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.499 ± 0.011
3.122 ± 0.014
4.916 ± 0.02
3.953 ± 0.02
7.36 ± 0.024
7.382 ± 0.026
5.583 ± 0.018
7.656 ± 0.02
1.201 ± 0.009
2.507 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here