Staphylococcus sp. DORA_6_22

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus; unclassified Staphylococcus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W1W5U8|W1W5U8_9STAP Competence protein ComGF OS=Staphylococcus sp. DORA_6_22 OX=1403935 GN=Q614_SASC00242G0006 PE=4 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.09MYY5 pKa = 11.19LYY7 pKa = 10.78DD8 pKa = 6.09DD9 pKa = 4.7EE10 pKa = 5.2EE11 pKa = 4.3QSQVQFVGFVGEE23 pKa = 3.94HH24 pKa = 4.99SRR26 pKa = 11.84YY27 pKa = 10.87DD28 pKa = 3.57LMLVQTDD35 pKa = 2.95RR36 pKa = 11.84HH37 pKa = 5.67YY38 pKa = 11.8GKK40 pKa = 8.15TLVLNMQTNQFGIIGSDD57 pKa = 3.67DD58 pKa = 3.61IEE60 pKa = 4.36EE61 pKa = 4.17EE62 pKa = 5.04GYY64 pKa = 10.1ISHH67 pKa = 7.21ILGVSKK73 pKa = 11.13EE74 pKa = 4.12EE75 pKa = 3.66ADD77 pKa = 4.92EE78 pKa = 4.52IIEE81 pKa = 3.98YY82 pKa = 10.51LNEE85 pKa = 4.26VIQQ88 pKa = 4.08

Molecular weight: 10.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W1W5H6|W1W5H6_9STAP MOSC protein OS=Staphylococcus sp. DORA_6_22 OX=1403935 GN=Q614_SASC00168G0043 PE=4 SV=1
MM1 pKa = 7.31IAVISMSGHH10 pKa = 5.12VWSQQVPLWSIIIRR24 pKa = 11.84TIHH27 pKa = 7.35LIGLTLWLGSLVYY40 pKa = 9.11LICYY44 pKa = 9.18AIKK47 pKa = 10.82VKK49 pKa = 10.36INQLTSVRR57 pKa = 11.84RR58 pKa = 11.84MLLKK62 pKa = 10.86VNIIAVFMLVFTGILMAIDD81 pKa = 3.56EE82 pKa = 4.96TNTLTLWNNVSAWSIYY98 pKa = 10.66LVIKK102 pKa = 9.87IAGIITMMLLGFYY115 pKa = 9.11QTMRR119 pKa = 11.84ALRR122 pKa = 11.84QRR124 pKa = 11.84QQVHH128 pKa = 5.87RR129 pKa = 11.84FALMTEE135 pKa = 4.42LLIGMILIFAGIMMSKK151 pKa = 10.59

Molecular weight: 17.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1603

0

1603

402552

20

2400

251.1

28.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.827 ± 0.064

0.644 ± 0.019

5.603 ± 0.062

6.61 ± 0.08

4.521 ± 0.057

6.139 ± 0.067

2.46 ± 0.033

8.962 ± 0.073

7.566 ± 0.083

9.317 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.027

5.717 ± 0.057

3.136 ± 0.035

4.164 ± 0.049

3.587 ± 0.048

6.181 ± 0.052

5.567 ± 0.045

6.475 ± 0.05

0.747 ± 0.022

3.942 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski