Thermovenabulum gondwanense
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2237 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A161Q9M0|A0A161Q9M0_9FIRM Uncharacterized protein OS=Thermovenabulum gondwanense OX=520767 GN=ATZ99_21200 PE=4 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.74 VFVDD6 pKa = 3.42 QDD8 pKa = 3.79 LCISCGLCIDD18 pKa = 4.45 TCPEE22 pKa = 3.86 VYY24 pKa = 9.92 SWNDD28 pKa = 3.2 DD29 pKa = 3.41 GKK31 pKa = 10.86 AQAIDD36 pKa = 3.82 SEE38 pKa = 5.02 VPADD42 pKa = 4.21 LEE44 pKa = 4.5 DD45 pKa = 3.71 EE46 pKa = 4.15 ARR48 pKa = 11.84 EE49 pKa = 3.95 ALEE52 pKa = 4.24 NCPSEE57 pKa = 4.59 AIKK60 pKa = 10.95 EE61 pKa = 4.17 EE62 pKa = 4.14
Molecular weight: 6.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.706
IPC_protein 3.63
Toseland 3.439
ProMoST 3.757
Dawson 3.617
Bjellqvist 3.846
Wikipedia 3.554
Rodwell 3.465
Grimsley 3.35
Solomon 3.579
Lehninger 3.541
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.554
Sillero 3.745
Patrickios 0.693
IPC_peptide 3.579
IPC2_peptide 3.719
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A161PW99|A0A161PW99_9FIRM Uncharacterized protein OS=Thermovenabulum gondwanense OX=520767 GN=ATZ99_16300 PE=4 SV=1
MM1 pKa = 7.8 KK2 pKa = 10.22 LRR4 pKa = 11.84 EE5 pKa = 4.53 SILTALLMAIGYY17 pKa = 8.03 VLHH20 pKa = 6.3 QVTPPIVAGMRR31 pKa = 11.84 PNFLLVMLFLSIFINPKK48 pKa = 8.46 PANALLAGFLGGVFAALSTTFPGGQIANIVDD79 pKa = 3.89 EE80 pKa = 5.75 LITSQVIALLVRR92 pKa = 11.84 VTPNLNRR99 pKa = 11.84 QIAVPIFTLLGTMISGAIFLSVAMIVVGALPTAFSTLYY137 pKa = 9.71 TVVVLPTAAINTVVGAILYY156 pKa = 9.39 NLARR160 pKa = 11.84 TALRR164 pKa = 11.84 TVVKK168 pKa = 9.89 FF169 pKa = 3.57
Molecular weight: 17.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 10.028
IPC_protein 10.847
Toseland 10.716
ProMoST 10.496
Dawson 10.847
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 11.038
Grimsley 10.921
Solomon 10.965
Lehninger 10.921
Nozaki 10.672
DTASelect 10.599
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.774
Patrickios 10.833
IPC_peptide 10.965
IPC2_peptide 9.516
IPC2.peptide.svr19 8.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2237
0
2237
679041
29
2326
303.5
34.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.72 ± 0.063
1.034 ± 0.021
4.823 ± 0.041
8.147 ± 0.064
4.601 ± 0.044
7.246 ± 0.051
1.312 ± 0.02
9.46 ± 0.058
8.883 ± 0.053
9.673 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.494 ± 0.025
4.687 ± 0.045
3.69 ± 0.033
2.212 ± 0.027
4.125 ± 0.038
5.26 ± 0.035
4.412 ± 0.039
7.019 ± 0.048
0.633 ± 0.016
3.572 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here