Shewanella algae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3935 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S2T8I8|A0A1S2T8I8_9GAMM Flagella-associated GTP-binding protein OS=Shewanella algae OX=38313 GN=BFS86_03580 PE=3 SV=1
MM1 pKa = 7.92KK2 pKa = 10.55LFQCNWLIRR11 pKa = 11.84ALGVVLIGGASLYY24 pKa = 10.29GASSLTPVNASFFPQQFDD42 pKa = 3.68QACMAEE48 pKa = 4.17QAGFALNCTANDD60 pKa = 3.45VRR62 pKa = 11.84VSKK65 pKa = 10.94VDD67 pKa = 3.26NVRR70 pKa = 11.84NLDD73 pKa = 3.19GTTPVEE79 pKa = 4.42CVLGDD84 pKa = 3.99DD85 pKa = 3.94VTFLADD91 pKa = 3.41VTITTTANQRR101 pKa = 11.84YY102 pKa = 9.03DD103 pKa = 3.48YY104 pKa = 11.06SVYY107 pKa = 10.48LPEE110 pKa = 5.14GSWSAQDD117 pKa = 3.46SDD119 pKa = 4.57PNNEE123 pKa = 3.81CSILLGRR130 pKa = 11.84TNGPGVDD137 pKa = 3.77LEE139 pKa = 4.64EE140 pKa = 4.98NLDD143 pKa = 3.7ACADD147 pKa = 3.31ISKK150 pKa = 10.47AAGYY154 pKa = 9.05DD155 pKa = 3.29ATHH158 pKa = 6.65VYY160 pKa = 10.35SGEE163 pKa = 4.13QITLFCRR170 pKa = 11.84DD171 pKa = 4.26DD172 pKa = 4.47DD173 pKa = 4.43NSGKK177 pKa = 10.66AEE179 pKa = 4.04FNYY182 pKa = 9.59CAAWHH187 pKa = 6.28NKK189 pKa = 7.81TGADD193 pKa = 3.32CSEE196 pKa = 4.5NNPAAPGTPSKK207 pKa = 10.53CRR209 pKa = 11.84CDD211 pKa = 4.08AFDD214 pKa = 3.88IDD216 pKa = 5.61VFIKK220 pKa = 10.44PNPPVINKK228 pKa = 9.68KK229 pKa = 10.55LITSNTQNEE238 pKa = 4.23PGGTYY243 pKa = 9.02TFEE246 pKa = 5.24LSFNNPNAQTSLFITSLSDD265 pKa = 3.43EE266 pKa = 4.27VDD268 pKa = 2.87IGADD272 pKa = 3.45GTYY275 pKa = 8.89DD276 pKa = 3.1TSLNLWSAGGPVGASDD292 pKa = 3.93GVYY295 pKa = 10.35LKK297 pKa = 10.83SSNCTQPANGGEE309 pKa = 4.08ILPSGSYY316 pKa = 9.21SCQFTVHH323 pKa = 6.39IVDD326 pKa = 4.6RR327 pKa = 11.84DD328 pKa = 3.93LPDD331 pKa = 4.07LAPNIEE337 pKa = 4.87LYY339 pKa = 10.97DD340 pKa = 4.12DD341 pKa = 5.3LIKK344 pKa = 10.86LALEE348 pKa = 4.76DD349 pKa = 4.23KK350 pKa = 11.17NGDD353 pKa = 3.79DD354 pKa = 4.87VIDD357 pKa = 4.32SNSCAAVNGIDD368 pKa = 5.23GEE370 pKa = 4.43HH371 pKa = 7.06CSNVVQVQVEE381 pKa = 4.22NLNPSITVLKK391 pKa = 8.8TANPDD396 pKa = 3.34QVPEE400 pKa = 4.05SGGNVTYY407 pKa = 8.62TVRR410 pKa = 11.84VNNTAEE416 pKa = 4.86AYY418 pKa = 9.07DD419 pKa = 3.83SPLEE423 pKa = 4.0LFYY426 pKa = 11.34LVDD429 pKa = 5.34DD430 pKa = 5.15KK431 pKa = 11.75FGDD434 pKa = 3.93LNGKK438 pKa = 8.81GSCSTYY444 pKa = 10.98LDD446 pKa = 3.27IAYY449 pKa = 9.93GSYY452 pKa = 10.25YY453 pKa = 9.79EE454 pKa = 4.51CSFTEE459 pKa = 5.36FISGTGAGSHH469 pKa = 6.02TNTVTAKK476 pKa = 10.54AYY478 pKa = 9.82DD479 pKa = 3.77DD480 pKa = 3.87EE481 pKa = 5.05MIEE484 pKa = 5.13AMAADD489 pKa = 3.94SATVVINDD497 pKa = 3.75IPSMITLDD505 pKa = 3.57KK506 pKa = 11.19SADD509 pKa = 3.61PTSVLEE515 pKa = 4.32TGDD518 pKa = 3.85DD519 pKa = 3.57LSIFRR524 pKa = 11.84DD525 pKa = 3.63VEE527 pKa = 4.22FTFLFGVKK535 pKa = 10.1DD536 pKa = 3.7QINGQNTVDD545 pKa = 3.06TVTFNTLDD553 pKa = 3.63DD554 pKa = 4.28SVFGDD559 pKa = 3.99LTAKK563 pKa = 10.81CEE565 pKa = 3.83VDD567 pKa = 3.57TVDD570 pKa = 3.86GLPYY574 pKa = 10.64GPAPLAGLMLDD585 pKa = 3.89PGHH588 pKa = 6.37NASCTITLQVQGNRR602 pKa = 11.84TDD604 pKa = 2.47IHH606 pKa = 6.42TNLATIYY613 pKa = 9.26GTDD616 pKa = 3.5EE617 pKa = 4.71DD618 pKa = 4.53GQAVDD623 pKa = 4.97AMDD626 pKa = 4.94DD627 pKa = 3.5ATVTFTPGSPAVDD640 pKa = 3.25MDD642 pKa = 4.49FAASMLVVLGMHH654 pKa = 6.49NADD657 pKa = 3.49VNNANLTMLTAAGVDD672 pKa = 3.73VFPGADD678 pKa = 2.99MPGFKK683 pKa = 10.36LINSGGTFEE692 pKa = 4.14GVNYY696 pKa = 9.72GSCALNHH703 pKa = 6.23PFGYY707 pKa = 8.89TGSGTEE713 pKa = 4.9DD714 pKa = 3.82YY715 pKa = 10.78FCAFTIEE722 pKa = 4.51FKK724 pKa = 10.8PGLEE728 pKa = 4.06NTDD731 pKa = 4.24PIVFLGDD738 pKa = 3.59VIVKK742 pKa = 9.45LVNSKK747 pKa = 10.77GDD749 pKa = 3.31EE750 pKa = 4.1STADD754 pKa = 3.19VTIQVGTNEE763 pKa = 3.96NN764 pKa = 3.4

Molecular weight: 81.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S2TVI2|A0A1S2TVI2_9GAMM Fe-S assembly protein IscX OS=Shewanella algae OX=38313 GN=BFS86_12180 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.31SLSLLQFPRR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 5.61PQALTIISRR22 pKa = 11.84ALASIIGGYY31 pKa = 10.17LIASLACALLAVSLPMSRR49 pKa = 11.84IDD51 pKa = 3.61STVCAMMLSFLIYY64 pKa = 9.01ALVVIRR70 pKa = 11.84VFCIKK75 pKa = 10.25RR76 pKa = 11.84SLRR79 pKa = 11.84AWQEE83 pKa = 3.89LLLLAAALFGVLKK96 pKa = 10.86LLGAA100 pKa = 4.7

Molecular weight: 10.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3935

0

3935

1298577

37

1980

330.0

36.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.846 ± 0.045

1.113 ± 0.016

5.358 ± 0.029

6.184 ± 0.035

3.831 ± 0.026

7.326 ± 0.034

2.147 ± 0.019

5.356 ± 0.03

4.725 ± 0.032

11.662 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.02

3.58 ± 0.027

4.255 ± 0.025

5.06 ± 0.037

5.213 ± 0.033

6.366 ± 0.03

4.711 ± 0.027

6.456 ± 0.034

1.337 ± 0.018

2.917 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski