Amino acid dipepetide frequency for Shewanella algae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.421AlaAla: 10.421 ± 0.114
1.211AlaCys: 1.211 ± 0.035
5.387AlaAsp: 5.387 ± 0.071
6.878AlaGlu: 6.878 ± 0.083
3.591AlaPhe: 3.591 ± 0.051
7.074AlaGly: 7.074 ± 0.09
1.722AlaHis: 1.722 ± 0.038
5.785AlaIle: 5.785 ± 0.074
5.213AlaLys: 5.213 ± 0.071
12.001AlaLeu: 12.001 ± 0.125
3.006AlaMet: 3.006 ± 0.055
3.572AlaAsn: 3.572 ± 0.062
3.597AlaPro: 3.597 ± 0.058
4.326AlaGln: 4.326 ± 0.066
4.839AlaArg: 4.839 ± 0.07
5.789AlaSer: 5.789 ± 0.069
4.289AlaThr: 4.289 ± 0.068
6.236AlaVal: 6.236 ± 0.071
1.174AlaTrp: 1.174 ± 0.028
2.35AlaTyr: 2.35 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
0.942CysAla: 0.942 ± 0.03
0.191CysCys: 0.191 ± 0.013
0.645CysAsp: 0.645 ± 0.024
0.648CysGlu: 0.648 ± 0.024
0.477CysPhe: 0.477 ± 0.019
0.956CysGly: 0.956 ± 0.027
0.5CysHis: 0.5 ± 0.033
0.561CysIle: 0.561 ± 0.021
0.359CysLys: 0.359 ± 0.014
1.104CysLeu: 1.104 ± 0.036
0.241CysMet: 0.241 ± 0.015
0.357CysAsn: 0.357 ± 0.019
0.528CysPro: 0.528 ± 0.026
0.583CysGln: 0.583 ± 0.021
0.697CysArg: 0.697 ± 0.025
0.744CysSer: 0.744 ± 0.028
0.423CysThr: 0.423 ± 0.018
0.64CysVal: 0.64 ± 0.024
0.152CysTrp: 0.152 ± 0.01
0.383CysTyr: 0.383 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.985AspAla: 4.985 ± 0.064
0.609AspCys: 0.609 ± 0.023
2.863AspAsp: 2.863 ± 0.06
3.726AspGlu: 3.726 ± 0.057
2.347AspPhe: 2.347 ± 0.049
3.953AspGly: 3.953 ± 0.069
0.899AspHis: 0.899 ± 0.027
3.451AspIle: 3.451 ± 0.056
3.378AspLys: 3.378 ± 0.059
4.963AspLeu: 4.963 ± 0.066
1.382AspMet: 1.382 ± 0.03
2.382AspAsn: 2.382 ± 0.047
2.326AspPro: 2.326 ± 0.041
1.638AspGln: 1.638 ± 0.038
2.423AspArg: 2.423 ± 0.054
3.498AspSer: 3.498 ± 0.059
2.65AspThr: 2.65 ± 0.041
3.054AspVal: 3.054 ± 0.048
0.98AspTrp: 0.98 ± 0.03
2.074AspTyr: 2.074 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
6.183GluAla: 6.183 ± 0.08
0.534GluCys: 0.534 ± 0.024
2.721GluAsp: 2.721 ± 0.059
3.349GluGlu: 3.349 ± 0.062
2.242GluPhe: 2.242 ± 0.038
3.807GluGly: 3.807 ± 0.057
1.482GluHis: 1.482 ± 0.038
3.299GluIle: 3.299 ± 0.06
2.712GluLys: 2.712 ± 0.055
8.362GluLeu: 8.362 ± 0.08
1.615GluMet: 1.615 ± 0.038
1.89GluAsn: 1.89 ± 0.038
2.366GluPro: 2.366 ± 0.05
4.525GluGln: 4.525 ± 0.072
3.791GluArg: 3.791 ± 0.061
3.273GluSer: 3.273 ± 0.053
2.936GluThr: 2.936 ± 0.049
4.308GluVal: 4.308 ± 0.068
0.709GluTrp: 0.709 ± 0.025
1.729GluTyr: 1.729 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
3.802PheAla: 3.802 ± 0.061
0.514PheCys: 0.514 ± 0.021
2.605PheAsp: 2.605 ± 0.044
2.406PheGlu: 2.406 ± 0.044
1.574PhePhe: 1.574 ± 0.039
3.237PheGly: 3.237 ± 0.062
0.777PheHis: 0.777 ± 0.026
2.346PheIle: 2.346 ± 0.039
1.716PheLys: 1.716 ± 0.035
3.082PheLeu: 3.082 ± 0.051
0.97PheMet: 0.97 ± 0.027
1.795PheAsn: 1.795 ± 0.042
1.398PhePro: 1.398 ± 0.032
1.19PheGln: 1.19 ± 0.03
1.716PheArg: 1.716 ± 0.035
3.038PheSer: 3.038 ± 0.055
1.876PheThr: 1.876 ± 0.036
2.366PheVal: 2.366 ± 0.046
0.56PheTrp: 0.56 ± 0.021
1.341PheTyr: 1.341 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
6.18GlyAla: 6.18 ± 0.081
0.996GlyCys: 0.996 ± 0.032
3.75GlyAsp: 3.75 ± 0.067
4.713GlyGlu: 4.713 ± 0.063
3.429GlyPhe: 3.429 ± 0.056
5.01GlyGly: 5.01 ± 0.082
1.702GlyHis: 1.702 ± 0.039
4.562GlyIle: 4.562 ± 0.061
4.168GlyLys: 4.168 ± 0.065
7.901GlyLeu: 7.901 ± 0.086
2.192GlyMet: 2.192 ± 0.04
2.671GlyAsn: 2.671 ± 0.061
1.994GlyPro: 1.994 ± 0.045
3.374GlyGln: 3.374 ± 0.051
3.92GlyArg: 3.92 ± 0.06
4.549GlySer: 4.549 ± 0.069
3.339GlyThr: 3.339 ± 0.055
5.136GlyVal: 5.136 ± 0.062
1.031GlyTrp: 1.031 ± 0.028
2.641GlyTyr: 2.641 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
1.57HisAla: 1.57 ± 0.037
0.365HisCys: 0.365 ± 0.019
0.992HisAsp: 0.992 ± 0.03
1.043HisGlu: 1.043 ± 0.023
1.067HisPhe: 1.067 ± 0.029
1.697HisGly: 1.697 ± 0.046
0.606HisHis: 0.606 ± 0.026
1.15HisIle: 1.15 ± 0.03
1.107HisLys: 1.107 ± 0.029
2.37HisLeu: 2.37 ± 0.047
0.491HisMet: 0.491 ± 0.019
0.773HisAsn: 0.773 ± 0.022
1.234HisPro: 1.234 ± 0.031
1.196HisGln: 1.196 ± 0.027
1.067HisArg: 1.067 ± 0.032
1.482HisSer: 1.482 ± 0.036
1.016HisThr: 1.016 ± 0.035
0.963HisVal: 0.963 ± 0.031
0.435HisTrp: 0.435 ± 0.018
0.846HisTyr: 0.846 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.065IleAla: 6.065 ± 0.076
0.634IleCys: 0.634 ± 0.023
3.434IleAsp: 3.434 ± 0.059
3.656IleGlu: 3.656 ± 0.053
1.722IlePhe: 1.722 ± 0.041
4.18IleGly: 4.18 ± 0.067
1.083IleHis: 1.083 ± 0.031
2.797IleIle: 2.797 ± 0.05
2.597IleLys: 2.597 ± 0.045
4.889IleLeu: 4.889 ± 0.08
1.117IleMet: 1.117 ± 0.03
2.396IleAsn: 2.396 ± 0.04
2.37IlePro: 2.37 ± 0.046
1.871IleGln: 1.871 ± 0.042
2.882IleArg: 2.882 ± 0.05
3.865IleSer: 3.865 ± 0.062
2.756IleThr: 2.756 ± 0.047
3.118IleVal: 3.118 ± 0.053
0.635IleTrp: 0.635 ± 0.023
1.493IleTyr: 1.493 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
5.276LysAla: 5.276 ± 0.066
0.315LysCys: 0.315 ± 0.02
2.472LysAsp: 2.472 ± 0.049
2.749LysGlu: 2.749 ± 0.053
1.26LysPhe: 1.26 ± 0.032
3.591LysGly: 3.591 ± 0.059
1.089LysHis: 1.089 ± 0.03
2.242LysIle: 2.242 ± 0.051
1.907LysLys: 1.907 ± 0.046
5.589LysLeu: 5.589 ± 0.066
1.134LysMet: 1.134 ± 0.029
1.328LysAsn: 1.328 ± 0.035
2.407LysPro: 2.407 ± 0.052
2.972LysGln: 2.972 ± 0.05
2.774LysArg: 2.774 ± 0.045
2.635LysSer: 2.635 ± 0.051
2.247LysThr: 2.247 ± 0.043
3.605LysVal: 3.605 ± 0.057
0.461LysTrp: 0.461 ± 0.02
1.197LysTyr: 1.197 ± 0.032
0.0LysXaa: 0.0 ± 0.0
Leu
12.804LeuAla: 12.804 ± 0.129
1.314LeuCys: 1.314 ± 0.037
6.294LeuAsp: 6.294 ± 0.082
7.075LeuGlu: 7.075 ± 0.075
4.244LeuPhe: 4.244 ± 0.07
8.391LeuGly: 8.391 ± 0.099
2.111LeuHis: 2.111 ± 0.043
5.445LeuIle: 5.445 ± 0.077
5.987LeuLys: 5.987 ± 0.07
14.212LeuLeu: 14.212 ± 0.206
2.888LeuMet: 2.888 ± 0.048
4.191LeuAsn: 4.191 ± 0.059
5.697LeuPro: 5.697 ± 0.083
5.022LeuGln: 5.022 ± 0.082
5.071LeuArg: 5.071 ± 0.068
8.3LeuSer: 8.3 ± 0.096
5.935LeuThr: 5.935 ± 0.078
7.354LeuVal: 7.354 ± 0.086
1.432LeuTrp: 1.432 ± 0.04
2.852LeuTyr: 2.852 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.992MetAla: 2.992 ± 0.057
0.17MetCys: 0.17 ± 0.013
1.279MetAsp: 1.279 ± 0.029
1.318MetGlu: 1.318 ± 0.032
0.765MetPhe: 0.765 ± 0.026
1.844MetGly: 1.844 ± 0.04
0.455MetHis: 0.455 ± 0.021
1.152MetIle: 1.152 ± 0.033
1.254MetLys: 1.254 ± 0.032
3.196MetLeu: 3.196 ± 0.055
0.721MetMet: 0.721 ± 0.025
0.901MetAsn: 0.901 ± 0.027
1.331MetPro: 1.331 ± 0.03
1.279MetGln: 1.279 ± 0.034
1.237MetArg: 1.237 ± 0.032
1.788MetSer: 1.788 ± 0.036
1.518MetThr: 1.518 ± 0.04
1.719MetVal: 1.719 ± 0.036
0.214MetTrp: 0.214 ± 0.013
0.443MetTyr: 0.443 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.297AsnAla: 3.297 ± 0.051
0.421AsnCys: 0.421 ± 0.019
1.866AsnAsp: 1.866 ± 0.043
1.909AsnGlu: 1.909 ± 0.037
1.278AsnPhe: 1.278 ± 0.027
2.734AsnGly: 2.734 ± 0.062
0.794AsnHis: 0.794 ± 0.027
2.055AsnIle: 2.055 ± 0.045
1.694AsnLys: 1.694 ± 0.038
3.759AsnLeu: 3.759 ± 0.061
0.906AsnMet: 0.906 ± 0.026
1.479AsnAsn: 1.479 ± 0.046
2.085AsnPro: 2.085 ± 0.038
1.894AsnGln: 1.894 ± 0.045
2.031AsnArg: 2.031 ± 0.042
2.227AsnSer: 2.227 ± 0.042
1.79AsnThr: 1.79 ± 0.043
1.901AsnVal: 1.901 ± 0.042
0.58AsnTrp: 0.58 ± 0.02
1.097AsnTyr: 1.097 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
3.914ProAla: 3.914 ± 0.058
0.361ProCys: 0.361 ± 0.017
2.43ProAsp: 2.43 ± 0.045
3.607ProGlu: 3.607 ± 0.054
1.684ProPhe: 1.684 ± 0.038
3.305ProGly: 3.305 ± 0.054
0.847ProHis: 0.847 ± 0.026
1.966ProIle: 1.966 ± 0.042
2.228ProLys: 2.228 ± 0.046
5.188ProLeu: 5.188 ± 0.07
1.172ProMet: 1.172 ± 0.032
1.341ProAsn: 1.341 ± 0.033
1.288ProPro: 1.288 ± 0.034
2.185ProGln: 2.185 ± 0.045
1.719ProArg: 1.719 ± 0.035
2.462ProSer: 2.462 ± 0.041
1.768ProThr: 1.768 ± 0.039
3.228ProVal: 3.228 ± 0.05
0.661ProTrp: 0.661 ± 0.027
1.196ProTyr: 1.196 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
5.56GlnAla: 5.56 ± 0.072
0.405GlnCys: 0.405 ± 0.018
2.201GlnAsp: 2.201 ± 0.045
2.601GlnGlu: 2.601 ± 0.053
1.625GlnPhe: 1.625 ± 0.035
3.78GlnGly: 3.78 ± 0.057
1.187GlnHis: 1.187 ± 0.031
2.319GlnIle: 2.319 ± 0.042
1.847GlnLys: 1.847 ± 0.041
6.805GlnLeu: 6.805 ± 0.101
1.201GlnMet: 1.201 ± 0.033
1.337GlnAsn: 1.337 ± 0.037
1.954GlnPro: 1.954 ± 0.039
4.178GlnGln: 4.178 ± 0.089
2.751GlnArg: 2.751 ± 0.049
3.0GlnSer: 3.0 ± 0.055
2.39GlnThr: 2.39 ± 0.054
3.328GlnVal: 3.328 ± 0.057
0.714GlnTrp: 0.714 ± 0.022
1.415GlnTyr: 1.415 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
4.259ArgAla: 4.259 ± 0.062
0.554ArgCys: 0.554 ± 0.016
2.776ArgAsp: 2.776 ± 0.05
3.378ArgGlu: 3.378 ± 0.054
2.399ArgPhe: 2.399 ± 0.039
3.12ArgGly: 3.12 ± 0.053
1.397ArgHis: 1.397 ± 0.036
3.053ArgIle: 3.053 ± 0.054
2.239ArgLys: 2.239 ± 0.047
6.564ArgLeu: 6.564 ± 0.08
1.261ArgMet: 1.261 ± 0.032
1.817ArgAsn: 1.817 ± 0.037
1.97ArgPro: 1.97 ± 0.039
3.06ArgGln: 3.06 ± 0.058
3.204ArgArg: 3.204 ± 0.06
2.889ArgSer: 2.889 ± 0.048
2.009ArgThr: 2.009 ± 0.042
3.522ArgVal: 3.522 ± 0.055
0.744ArgTrp: 0.744 ± 0.023
1.921ArgTyr: 1.921 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
5.986SerAla: 5.986 ± 0.074
0.755SerCys: 0.755 ± 0.031
3.262SerAsp: 3.262 ± 0.051
3.732SerGlu: 3.732 ± 0.057
2.691SerPhe: 2.691 ± 0.045
5.397SerGly: 5.397 ± 0.067
1.537SerHis: 1.537 ± 0.04
3.2SerIle: 3.2 ± 0.056
2.638SerLys: 2.638 ± 0.05
7.671SerLeu: 7.671 ± 0.097
1.572SerMet: 1.572 ± 0.031
2.168SerAsn: 2.168 ± 0.047
2.636SerPro: 2.636 ± 0.051
3.317SerGln: 3.317 ± 0.059
3.622SerArg: 3.622 ± 0.053
3.988SerSer: 3.988 ± 0.064
2.541SerThr: 2.541 ± 0.05
4.028SerVal: 4.028 ± 0.057
0.937SerTrp: 0.937 ± 0.028
1.981SerTyr: 1.981 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
4.423ThrAla: 4.423 ± 0.069
0.46ThrCys: 0.46 ± 0.024
2.668ThrAsp: 2.668 ± 0.049
3.23ThrGlu: 3.23 ± 0.051
1.575ThrPhe: 1.575 ± 0.04
3.875ThrGly: 3.875 ± 0.058
0.953ThrHis: 0.953 ± 0.023
2.319ThrIle: 2.319 ± 0.044
1.686ThrLys: 1.686 ± 0.038
6.306ThrLeu: 6.306 ± 0.073
0.997ThrMet: 0.997 ± 0.028
1.441ThrAsn: 1.441 ± 0.034
2.68ThrPro: 2.68 ± 0.05
2.413ThrGln: 2.413 ± 0.047
2.275ThrArg: 2.275 ± 0.046
2.755ThrSer: 2.755 ± 0.05
2.198ThrThr: 2.198 ± 0.055
3.19ThrVal: 3.19 ± 0.059
0.521ThrTrp: 0.521 ± 0.021
1.149ThrTyr: 1.149 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
6.483ValAla: 6.483 ± 0.081
0.749ValCys: 0.749 ± 0.024
3.742ValAsp: 3.742 ± 0.061
3.957ValGlu: 3.957 ± 0.058
2.443ValPhe: 2.443 ± 0.046
4.239ValGly: 4.239 ± 0.065
1.12ValHis: 1.12 ± 0.034
3.927ValIle: 3.927 ± 0.055
3.096ValLys: 3.096 ± 0.061
6.93ValLeu: 6.93 ± 0.085
1.807ValMet: 1.807 ± 0.039
2.591ValAsn: 2.591 ± 0.051
2.698ValPro: 2.698 ± 0.049
2.218ValGln: 2.218 ± 0.038
3.09ValArg: 3.09 ± 0.054
4.686ValSer: 4.686 ± 0.061
3.737ValThr: 3.737 ± 0.063
4.534ValVal: 4.534 ± 0.078
0.764ValTrp: 0.764 ± 0.023
1.746ValTyr: 1.746 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
0.923TrpAla: 0.923 ± 0.028
0.179TrpCys: 0.179 ± 0.012
0.65TrpAsp: 0.65 ± 0.023
0.495TrpGlu: 0.495 ± 0.021
0.578TrpPhe: 0.578 ± 0.023
0.845TrpGly: 0.845 ± 0.029
0.438TrpHis: 0.438 ± 0.016
0.584TrpIle: 0.584 ± 0.023
0.389TrpLys: 0.389 ± 0.017
2.101TrpLeu: 2.101 ± 0.052
0.33TrpMet: 0.33 ± 0.017
0.379TrpAsn: 0.379 ± 0.019
0.588TrpPro: 0.588 ± 0.02
1.412TrpGln: 1.412 ± 0.039
0.849TrpArg: 0.849 ± 0.031
0.757TrpSer: 0.757 ± 0.026
0.448TrpThr: 0.448 ± 0.02
0.825TrpVal: 0.825 ± 0.025
0.193TrpTrp: 0.193 ± 0.011
0.408TrpTyr: 0.408 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.396TyrAla: 2.396 ± 0.051
0.394TyrCys: 0.394 ± 0.017
1.544TyrAsp: 1.544 ± 0.042
1.365TyrGlu: 1.365 ± 0.033
1.317TyrPhe: 1.317 ± 0.035
2.221TyrGly: 2.221 ± 0.042
0.772TyrHis: 0.772 ± 0.024
1.348TyrIle: 1.348 ± 0.035
1.037TyrLys: 1.037 ± 0.028
3.626TyrLeu: 3.626 ± 0.052
0.572TyrMet: 0.572 ± 0.021
0.988TyrAsn: 0.988 ± 0.035
1.402TyrPro: 1.402 ± 0.033
1.947TyrGln: 1.947 ± 0.048
2.178TyrArg: 2.178 ± 0.046
1.933TyrSer: 1.933 ± 0.04
1.246TyrThr: 1.246 ± 0.03
1.503TyrVal: 1.503 ± 0.033
0.473TyrTrp: 0.473 ± 0.019
0.913TyrTyr: 0.913 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3935 proteins (1298577 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski