Streptomyces sp. SID13031

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7625 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B2TX50|A0A6B2TX50_9ACTN TIGR03667 family PPOX class F420-dependent oxidoreductase OS=Streptomyces sp. SID13031 OX=2706046 GN=G3I17_13095 PE=4 SV=1
MM1 pKa = 6.96GWYY4 pKa = 9.47SVRR7 pKa = 11.84CIFQLNGIRR16 pKa = 11.84ADD18 pKa = 3.53DD19 pKa = 3.54SPYY22 pKa = 10.89EE23 pKa = 3.97EE24 pKa = 6.32RR25 pKa = 11.84ITLWRR30 pKa = 11.84ADD32 pKa = 4.11DD33 pKa = 3.9FDD35 pKa = 4.23TAIEE39 pKa = 3.94LAEE42 pKa = 4.44NEE44 pKa = 4.05ALDD47 pKa = 4.51YY48 pKa = 11.39IEE50 pKa = 4.78DD51 pKa = 4.01TDD53 pKa = 3.61WAYY56 pKa = 11.38LGLAQCFYY64 pKa = 11.02LGDD67 pKa = 4.35DD68 pKa = 3.62VTQILQGTEE77 pKa = 4.13VYY79 pKa = 10.93SLIRR83 pKa = 11.84SNSLPPEE90 pKa = 4.58KK91 pKa = 10.74YY92 pKa = 9.99LDD94 pKa = 3.63SFFDD98 pKa = 3.41TGTEE102 pKa = 4.1HH103 pKa = 6.79QRR105 pKa = 11.84STDD108 pKa = 3.0

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B2TZC5|A0A6B2TZC5_9ACTN Carbohydrate-binding protein OS=Streptomyces sp. SID13031 OX=2706046 GN=G3I17_13970 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.33GRR40 pKa = 11.84QRR42 pKa = 11.84LAAA45 pKa = 4.56

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7625

0

7625

2496950

29

5015

327.5

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.681 ± 0.036

0.702 ± 0.007

5.981 ± 0.024

5.38 ± 0.026

2.948 ± 0.014

8.965 ± 0.026

2.028 ± 0.014

4.024 ± 0.018

2.608 ± 0.025

10.489 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.7 ± 0.011

2.256 ± 0.02

5.667 ± 0.021

3.208 ± 0.017

6.896 ± 0.03

5.64 ± 0.021

6.409 ± 0.028

8.568 ± 0.027

1.586 ± 0.012

2.264 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski