Amino acid dipepetide frequency for Streptomyces sp. SID13031

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.803AlaAla: 18.803 ± 0.141
0.877AlaCys: 0.877 ± 0.021
7.983AlaAsp: 7.983 ± 0.062
7.885AlaGlu: 7.885 ± 0.069
3.598AlaPhe: 3.598 ± 0.042
12.384AlaGly: 12.384 ± 0.083
2.168AlaHis: 2.168 ± 0.028
5.14AlaIle: 5.14 ± 0.049
3.564AlaLys: 3.564 ± 0.05
12.845AlaLeu: 12.845 ± 0.09
2.421AlaMet: 2.421 ± 0.028
2.613AlaAsn: 2.613 ± 0.042
5.585AlaPro: 5.585 ± 0.06
3.783AlaGln: 3.783 ± 0.039
7.774AlaArg: 7.774 ± 0.063
6.214AlaSer: 6.214 ± 0.056
7.707AlaThr: 7.707 ± 0.063
11.105AlaVal: 11.105 ± 0.084
1.771AlaTrp: 1.771 ± 0.026
2.589AlaTyr: 2.589 ± 0.031
0.0AlaXaa: 0.0 ± 0.0
Cys
0.788CysAla: 0.788 ± 0.02
0.088CysCys: 0.088 ± 0.006
0.426CysAsp: 0.426 ± 0.014
0.373CysGlu: 0.373 ± 0.012
0.217CysPhe: 0.217 ± 0.01
0.802CysGly: 0.802 ± 0.018
0.159CysHis: 0.159 ± 0.008
0.223CysIle: 0.223 ± 0.009
0.144CysLys: 0.144 ± 0.008
0.655CysLeu: 0.655 ± 0.017
0.115CysMet: 0.115 ± 0.007
0.181CysAsn: 0.181 ± 0.009
0.413CysPro: 0.413 ± 0.014
0.195CysGln: 0.195 ± 0.01
0.481CysArg: 0.481 ± 0.016
0.465CysSer: 0.465 ± 0.017
0.471CysThr: 0.471 ± 0.015
0.558CysVal: 0.558 ± 0.015
0.11CysTrp: 0.11 ± 0.007
0.16CysTyr: 0.16 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
6.891AspAla: 6.891 ± 0.062
0.372AspCys: 0.372 ± 0.013
3.713AspAsp: 3.713 ± 0.046
3.902AspGlu: 3.902 ± 0.053
1.706AspPhe: 1.706 ± 0.025
6.117AspGly: 6.117 ± 0.063
1.407AspHis: 1.407 ± 0.026
1.969AspIle: 1.969 ± 0.029
1.487AspLys: 1.487 ± 0.028
6.929AspLeu: 6.929 ± 0.057
0.676AspMet: 0.676 ± 0.017
1.221AspAsn: 1.221 ± 0.023
4.251AspPro: 4.251 ± 0.044
2.139AspGln: 2.139 ± 0.032
4.36AspArg: 4.36 ± 0.047
2.858AspSer: 2.858 ± 0.036
2.739AspThr: 2.739 ± 0.034
4.734AspVal: 4.734 ± 0.044
1.052AspTrp: 1.052 ± 0.022
1.283AspTyr: 1.283 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
6.492GluAla: 6.492 ± 0.067
0.298GluCys: 0.298 ± 0.01
2.483GluAsp: 2.483 ± 0.037
2.72GluGlu: 2.72 ± 0.036
1.632GluPhe: 1.632 ± 0.029
3.532GluGly: 3.532 ± 0.038
1.36GluHis: 1.36 ± 0.024
2.499GluIle: 2.499 ± 0.042
1.401GluLys: 1.401 ± 0.022
7.293GluLeu: 7.293 ± 0.072
0.858GluMet: 0.858 ± 0.019
1.084GluAsn: 1.084 ± 0.021
3.077GluPro: 3.077 ± 0.047
2.355GluGln: 2.355 ± 0.029
4.37GluArg: 4.37 ± 0.051
2.78GluSer: 2.78 ± 0.037
2.81GluThr: 2.81 ± 0.035
4.718GluVal: 4.718 ± 0.044
0.803GluTrp: 0.803 ± 0.02
1.23GluTyr: 1.23 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.815PheAla: 3.815 ± 0.041
0.28PheCys: 0.28 ± 0.011
2.185PheAsp: 2.185 ± 0.034
1.594PheGlu: 1.594 ± 0.026
0.909PhePhe: 0.909 ± 0.023
3.222PheGly: 3.222 ± 0.038
0.615PheHis: 0.615 ± 0.017
0.944PheIle: 0.944 ± 0.024
0.695PheLys: 0.695 ± 0.017
2.634PheLeu: 2.634 ± 0.037
0.391PheMet: 0.391 ± 0.013
0.737PheAsn: 0.737 ± 0.019
1.384PhePro: 1.384 ± 0.024
0.799PheGln: 0.799 ± 0.019
1.743PheArg: 1.743 ± 0.029
1.706PheSer: 1.706 ± 0.028
2.084PheThr: 2.084 ± 0.03
2.577PheVal: 2.577 ± 0.033
0.481PheTrp: 0.481 ± 0.015
0.685PheTyr: 0.685 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
9.474GlyAla: 9.474 ± 0.071
0.766GlyCys: 0.766 ± 0.02
4.965GlyAsp: 4.965 ± 0.056
4.536GlyGlu: 4.536 ± 0.041
3.055GlyPhe: 3.055 ± 0.038
7.694GlyGly: 7.694 ± 0.079
1.877GlyHis: 1.877 ± 0.028
4.033GlyIle: 4.033 ± 0.042
2.946GlyLys: 2.946 ± 0.042
9.462GlyLeu: 9.462 ± 0.076
1.741GlyMet: 1.741 ± 0.027
2.25GlyAsn: 2.25 ± 0.043
4.382GlyPro: 4.382 ± 0.05
3.075GlyGln: 3.075 ± 0.044
6.16GlyArg: 6.16 ± 0.061
5.623GlySer: 5.623 ± 0.055
5.837GlyThr: 5.837 ± 0.062
7.541GlyVal: 7.541 ± 0.056
1.823GlyTrp: 1.823 ± 0.03
2.415GlyTyr: 2.415 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.194HisAla: 2.194 ± 0.029
0.177HisCys: 0.177 ± 0.009
1.229HisAsp: 1.229 ± 0.025
1.12HisGlu: 1.12 ± 0.023
0.623HisPhe: 0.623 ± 0.016
1.924HisGly: 1.924 ± 0.028
0.608HisHis: 0.608 ± 0.016
0.602HisIle: 0.602 ± 0.014
0.397HisLys: 0.397 ± 0.013
2.334HisLeu: 2.334 ± 0.03
0.25HisMet: 0.25 ± 0.01
0.44HisAsn: 0.44 ± 0.015
1.499HisPro: 1.499 ± 0.026
0.732HisGln: 0.732 ± 0.018
1.646HisArg: 1.646 ± 0.028
1.035HisSer: 1.035 ± 0.024
1.093HisThr: 1.093 ± 0.021
1.536HisVal: 1.536 ± 0.026
0.38HisTrp: 0.38 ± 0.012
0.461HisTyr: 0.461 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
5.747IleAla: 5.747 ± 0.058
0.329IleCys: 0.329 ± 0.013
2.903IleAsp: 2.903 ± 0.037
2.517IleGlu: 2.517 ± 0.036
1.028IlePhe: 1.028 ± 0.024
4.376IleGly: 4.376 ± 0.052
0.752IleHis: 0.752 ± 0.018
1.242IleIle: 1.242 ± 0.025
0.981IleLys: 0.981 ± 0.019
3.095IleLeu: 3.095 ± 0.042
0.472IleMet: 0.472 ± 0.014
0.938IleAsn: 0.938 ± 0.021
2.187IlePro: 2.187 ± 0.033
1.03IleGln: 1.03 ± 0.021
2.603IleArg: 2.603 ± 0.034
2.396IleSer: 2.396 ± 0.03
2.827IleThr: 2.827 ± 0.037
3.487IleVal: 3.487 ± 0.039
0.509IleTrp: 0.509 ± 0.016
0.824IleTyr: 0.824 ± 0.018
0.0IleXaa: 0.0 ± 0.0
Lys
3.541LysAla: 3.541 ± 0.041
0.128LysCys: 0.128 ± 0.008
1.392LysAsp: 1.392 ± 0.026
1.098LysGlu: 1.098 ± 0.021
0.672LysPhe: 0.672 ± 0.016
1.793LysGly: 1.793 ± 0.033
0.532LysHis: 0.532 ± 0.016
1.114LysIle: 1.114 ± 0.023
0.893LysLys: 0.893 ± 0.029
2.888LysLeu: 2.888 ± 0.038
0.441LysMet: 0.441 ± 0.012
0.666LysAsn: 0.666 ± 0.018
1.818LysPro: 1.818 ± 0.037
0.926LysGln: 0.926 ± 0.02
1.498LysArg: 1.498 ± 0.027
1.472LysSer: 1.472 ± 0.025
1.759LysThr: 1.759 ± 0.03
2.447LysVal: 2.447 ± 0.035
0.342LysTrp: 0.342 ± 0.012
0.662LysTyr: 0.662 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
14.649LeuAla: 14.649 ± 0.096
0.682LeuCys: 0.682 ± 0.018
6.705LeuAsp: 6.705 ± 0.059
5.051LeuGlu: 5.051 ± 0.047
2.709LeuPhe: 2.709 ± 0.038
9.2LeuGly: 9.2 ± 0.078
2.11LeuHis: 2.11 ± 0.033
4.336LeuIle: 4.336 ± 0.054
2.442LeuLys: 2.442 ± 0.034
10.886LeuLeu: 10.886 ± 0.113
1.627LeuMet: 1.627 ± 0.03
2.134LeuAsn: 2.134 ± 0.03
6.188LeuPro: 6.188 ± 0.055
2.94LeuGln: 2.94 ± 0.035
7.631LeuArg: 7.631 ± 0.06
5.853LeuSer: 5.853 ± 0.047
7.265LeuThr: 7.265 ± 0.056
9.23LeuVal: 9.23 ± 0.078
1.304LeuTrp: 1.304 ± 0.027
1.947LeuTyr: 1.947 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.095MetAla: 2.095 ± 0.03
0.092MetCys: 0.092 ± 0.007
0.796MetAsp: 0.796 ± 0.017
0.68MetGlu: 0.68 ± 0.019
0.505MetPhe: 0.505 ± 0.013
1.119MetGly: 1.119 ± 0.022
0.352MetHis: 0.352 ± 0.011
0.792MetIle: 0.792 ± 0.018
0.491MetLys: 0.491 ± 0.014
1.75MetLeu: 1.75 ± 0.028
0.31MetMet: 0.31 ± 0.012
0.461MetAsn: 0.461 ± 0.015
1.06MetPro: 1.06 ± 0.019
0.46MetGln: 0.46 ± 0.014
1.23MetArg: 1.23 ± 0.024
1.379MetSer: 1.379 ± 0.024
1.579MetThr: 1.579 ± 0.023
1.343MetVal: 1.343 ± 0.026
0.191MetTrp: 0.191 ± 0.008
0.315MetTyr: 0.315 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.566AsnAla: 2.566 ± 0.033
0.201AsnCys: 0.201 ± 0.009
1.275AsnAsp: 1.275 ± 0.024
1.017AsnGlu: 1.017 ± 0.024
0.643AsnPhe: 0.643 ± 0.017
2.39AsnGly: 2.39 ± 0.039
0.499AsnHis: 0.499 ± 0.015
0.765AsnIle: 0.765 ± 0.019
0.554AsnLys: 0.554 ± 0.015
2.347AsnLeu: 2.347 ± 0.032
0.332AsnMet: 0.332 ± 0.012
0.651AsnAsn: 0.651 ± 0.022
1.711AsnPro: 1.711 ± 0.029
0.788AsnGln: 0.788 ± 0.021
1.487AsnArg: 1.487 ± 0.028
1.261AsnSer: 1.261 ± 0.028
1.425AsnThr: 1.425 ± 0.031
1.643AsnVal: 1.643 ± 0.026
0.427AsnTrp: 0.427 ± 0.015
0.578AsnTyr: 0.578 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
7.786ProAla: 7.786 ± 0.061
0.302ProCys: 0.302 ± 0.012
4.159ProAsp: 4.159 ± 0.039
3.666ProGlu: 3.666 ± 0.042
1.501ProPhe: 1.501 ± 0.029
5.648ProGly: 5.648 ± 0.057
1.03ProHis: 1.03 ± 0.019
2.022ProIle: 2.022 ± 0.031
1.421ProLys: 1.421 ± 0.026
4.941ProLeu: 4.941 ± 0.049
0.954ProMet: 0.954 ± 0.02
1.269ProAsn: 1.269 ± 0.024
3.079ProPro: 3.079 ± 0.052
1.702ProGln: 1.702 ± 0.029
3.055ProArg: 3.055 ± 0.04
3.246ProSer: 3.246 ± 0.041
3.726ProThr: 3.726 ± 0.053
4.954ProVal: 4.954 ± 0.055
0.931ProTrp: 0.931 ± 0.022
1.274ProTyr: 1.274 ± 0.023
0.0ProXaa: 0.0 ± 0.0
Gln
4.197GlnAla: 4.197 ± 0.047
0.171GlnCys: 0.171 ± 0.008
1.374GlnAsp: 1.374 ± 0.026
1.35GlnGlu: 1.35 ± 0.023
0.919GlnPhe: 0.919 ± 0.019
2.224GlnGly: 2.224 ± 0.032
0.685GlnHis: 0.685 ± 0.017
1.407GlnIle: 1.407 ± 0.022
0.729GlnLys: 0.729 ± 0.018
3.979GlnLeu: 3.979 ± 0.043
0.514GlnMet: 0.514 ± 0.015
0.696GlnAsn: 0.696 ± 0.018
2.028GlnPro: 2.028 ± 0.032
1.484GlnGln: 1.484 ± 0.03
2.492GlnArg: 2.492 ± 0.037
1.62GlnSer: 1.62 ± 0.028
1.845GlnThr: 1.845 ± 0.03
2.95GlnVal: 2.95 ± 0.037
0.546GlnTrp: 0.546 ± 0.015
0.869GlnTyr: 0.869 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
7.55ArgAla: 7.55 ± 0.065
0.465ArgCys: 0.465 ± 0.016
3.747ArgAsp: 3.747 ± 0.045
3.828ArgGlu: 3.828 ± 0.049
2.199ArgPhe: 2.199 ± 0.031
4.814ArgGly: 4.814 ± 0.041
1.525ArgHis: 1.525 ± 0.027
3.263ArgIle: 3.263 ± 0.037
1.757ArgLys: 1.757 ± 0.027
7.721ArgLeu: 7.721 ± 0.067
1.547ArgMet: 1.547 ± 0.024
1.527ArgAsn: 1.527 ± 0.027
3.863ArgPro: 3.863 ± 0.044
2.358ArgGln: 2.358 ± 0.03
6.272ArgArg: 6.272 ± 0.068
3.999ArgSer: 3.999 ± 0.044
4.333ArgThr: 4.333 ± 0.045
5.148ArgVal: 5.148 ± 0.055
1.269ArgTrp: 1.269 ± 0.028
1.769ArgTyr: 1.769 ± 0.026
0.0ArgXaa: 0.0 ± 0.0
Ser
6.751SerAla: 6.751 ± 0.059
0.429SerCys: 0.429 ± 0.015
2.952SerAsp: 2.952 ± 0.041
2.616SerGlu: 2.616 ± 0.038
1.798SerPhe: 1.798 ± 0.03
5.943SerGly: 5.943 ± 0.05
1.024SerHis: 1.024 ± 0.02
2.282SerIle: 2.282 ± 0.033
1.422SerLys: 1.422 ± 0.023
5.414SerLeu: 5.414 ± 0.049
1.252SerMet: 1.252 ± 0.023
1.284SerAsn: 1.284 ± 0.026
3.171SerPro: 3.171 ± 0.041
1.654SerGln: 1.654 ± 0.03
3.633SerArg: 3.633 ± 0.04
3.579SerSer: 3.579 ± 0.045
4.003SerThr: 4.003 ± 0.047
4.572SerVal: 4.572 ± 0.048
1.083SerTrp: 1.083 ± 0.022
1.537SerTyr: 1.537 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
8.415ThrAla: 8.415 ± 0.077
0.43ThrCys: 0.43 ± 0.014
3.67ThrAsp: 3.67 ± 0.04
3.247ThrGlu: 3.247 ± 0.036
1.977ThrPhe: 1.977 ± 0.035
6.362ThrGly: 6.362 ± 0.062
1.113ThrHis: 1.113 ± 0.023
2.62ThrIle: 2.62 ± 0.037
1.668ThrLys: 1.668 ± 0.033
5.854ThrLeu: 5.854 ± 0.048
1.083ThrMet: 1.083 ± 0.022
1.461ThrAsn: 1.461 ± 0.027
4.177ThrPro: 4.177 ± 0.054
1.719ThrGln: 1.719 ± 0.032
3.592ThrArg: 3.592 ± 0.034
3.749ThrSer: 3.749 ± 0.041
4.63ThrThr: 4.63 ± 0.063
5.814ThrVal: 5.814 ± 0.053
0.975ThrTrp: 0.975 ± 0.023
1.538ThrTyr: 1.538 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
10.92ValAla: 10.92 ± 0.079
0.601ValCys: 0.601 ± 0.017
5.33ValAsp: 5.33 ± 0.047
4.78ValGlu: 4.78 ± 0.044
2.455ValPhe: 2.455 ± 0.032
6.791ValGly: 6.791 ± 0.057
1.651ValHis: 1.651 ± 0.027
3.721ValIle: 3.721 ± 0.044
2.113ValLys: 2.113 ± 0.033
9.495ValLeu: 9.495 ± 0.079
1.443ValMet: 1.443 ± 0.027
1.924ValAsn: 1.924 ± 0.031
4.765ValPro: 4.765 ± 0.041
2.424ValGln: 2.424 ± 0.027
5.898ValArg: 5.898 ± 0.057
4.753ValSer: 4.753 ± 0.048
5.607ValThr: 5.607 ± 0.058
8.367ValVal: 8.367 ± 0.071
1.105ValTrp: 1.105 ± 0.023
1.538ValTyr: 1.538 ± 0.025
0.0ValXaa: 0.0 ± 0.0
Trp
1.546TrpAla: 1.546 ± 0.027
0.126TrpCys: 0.126 ± 0.007
0.826TrpAsp: 0.826 ± 0.021
0.649TrpGlu: 0.649 ± 0.016
0.586TrpPhe: 0.586 ± 0.016
1.041TrpGly: 1.041 ± 0.025
0.38TrpHis: 0.38 ± 0.012
0.694TrpIle: 0.694 ± 0.016
0.463TrpLys: 0.463 ± 0.014
1.925TrpLeu: 1.925 ± 0.029
0.328TrpMet: 0.328 ± 0.01
0.479TrpAsn: 0.479 ± 0.015
0.858TrpPro: 0.858 ± 0.018
0.695TrpGln: 0.695 ± 0.017
1.217TrpArg: 1.217 ± 0.025
1.123TrpSer: 1.123 ± 0.024
1.084TrpThr: 1.084 ± 0.023
1.097TrpVal: 1.097 ± 0.022
0.358TrpTrp: 0.358 ± 0.012
0.386TrpTyr: 0.386 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.591TyrAla: 2.591 ± 0.03
0.208TyrCys: 0.208 ± 0.009
1.698TyrAsp: 1.698 ± 0.031
1.167TyrGlu: 1.167 ± 0.024
0.748TyrPhe: 0.748 ± 0.018
2.277TyrGly: 2.277 ± 0.035
0.433TyrHis: 0.433 ± 0.012
0.574TyrIle: 0.574 ± 0.014
0.513TyrLys: 0.513 ± 0.014
2.443TyrLeu: 2.443 ± 0.031
0.242TyrMet: 0.242 ± 0.011
0.545TyrAsn: 0.545 ± 0.018
1.169TyrPro: 1.169 ± 0.021
0.824TyrGln: 0.824 ± 0.019
1.813TyrArg: 1.813 ± 0.03
1.287TyrSer: 1.287 ± 0.023
1.27TyrThr: 1.27 ± 0.026
1.858TyrVal: 1.858 ± 0.028
0.4TyrTrp: 0.4 ± 0.013
0.583TyrTyr: 0.583 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7625 proteins (2496950 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski