Lactobacillus phage LfeSau

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1ENB0|A0A0A1ENB0_9CAUD DNA-binding protein OS=Lactobacillus phage LfeSau OX=1567453 GN=LfeSau_32 PE=4 SV=1
MM1 pKa = 7.68IKK3 pKa = 10.13QFTVVDD9 pKa = 4.03GDD11 pKa = 3.66KK12 pKa = 11.15ADD14 pKa = 3.71YY15 pKa = 10.43VYY17 pKa = 10.92SGHH20 pKa = 6.4EE21 pKa = 3.74FAFVCDD27 pKa = 3.78EE28 pKa = 3.87QDD30 pKa = 2.44IDD32 pKa = 4.21LAYY35 pKa = 10.64DD36 pKa = 3.54VLAEE40 pKa = 4.02SLDD43 pKa = 3.92LVSEE47 pKa = 4.65DD48 pKa = 5.6DD49 pKa = 6.57LIDD52 pKa = 3.8FQNAQDD58 pKa = 4.54DD59 pKa = 4.33DD60 pKa = 4.74VPTLLDD66 pKa = 3.87DD67 pKa = 3.73MKK69 pKa = 11.62KK70 pKa = 9.47MGFIDD75 pKa = 3.79QWEE78 pKa = 4.16AWEE81 pKa = 4.32VEE83 pKa = 4.17

Molecular weight: 9.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1ENQ9|A0A0A1ENQ9_9CAUD Tape measure protein OS=Lactobacillus phage LfeSau OX=1567453 GN=LfeSau_16 PE=4 SV=1
MM1 pKa = 7.58ARR3 pKa = 11.84ITKK6 pKa = 9.9IEE8 pKa = 3.82GLEE11 pKa = 3.86EE12 pKa = 3.88LKK14 pKa = 11.04AGLKK18 pKa = 10.52GRR20 pKa = 11.84MNLAPVADD28 pKa = 3.98VVKK31 pKa = 10.77KK32 pKa = 10.09HH33 pKa = 5.35GAQLSSRR40 pKa = 11.84TQSNMNAAYY49 pKa = 10.03VHH51 pKa = 6.79GYY53 pKa = 7.27STGMTRR59 pKa = 11.84RR60 pKa = 11.84SVTPVFSNAGMTVSVGPTTDD80 pKa = 3.16YY81 pKa = 10.99FPYY84 pKa = 10.53LEE86 pKa = 4.36YY87 pKa = 9.62GTRR90 pKa = 11.84FMNAMPTLKK99 pKa = 10.19PAFDD103 pKa = 3.86VQSVMFINEE112 pKa = 4.55LKK114 pKa = 11.09GLMQQ118 pKa = 4.86

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10006

44

1155

200.1

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.615 ± 0.667

0.52 ± 0.095

6.986 ± 0.415

5.797 ± 0.48

3.788 ± 0.215

7.076 ± 0.511

1.219 ± 0.148

5.846 ± 0.267

6.806 ± 0.424

7.945 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.217

5.487 ± 0.323

3.448 ± 0.257

4.747 ± 0.213

4.397 ± 0.381

5.956 ± 0.352

6.646 ± 0.355

7.246 ± 0.31

1.349 ± 0.18

3.478 ± 0.326
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski