Amino acid dipepetide frequency for Lactobacillus phage LfeSau

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.896AlaAla: 7.896 ± 1.838
0.2AlaCys: 0.2 ± 0.107
5.897AlaAsp: 5.897 ± 0.827
4.198AlaGlu: 4.198 ± 0.647
3.998AlaPhe: 3.998 ± 0.515
6.497AlaGly: 6.497 ± 0.731
0.8AlaHis: 0.8 ± 0.204
6.497AlaIle: 6.497 ± 0.847
4.798AlaLys: 4.798 ± 0.852
6.897AlaLeu: 6.897 ± 0.853
2.799AlaMet: 2.799 ± 0.793
4.198AlaAsn: 4.198 ± 0.693
3.498AlaPro: 3.498 ± 0.673
3.498AlaGln: 3.498 ± 0.579
3.298AlaArg: 3.298 ± 0.609
4.698AlaSer: 4.698 ± 0.544
6.497AlaThr: 6.497 ± 0.787
5.097AlaVal: 5.097 ± 0.955
1.699AlaTrp: 1.699 ± 0.455
3.198AlaTyr: 3.198 ± 0.714
0.0AlaXaa: 0.0 ± 0.0
Cys
0.2CysAla: 0.2 ± 0.141
0.0CysCys: 0.0 ± 0.0
0.3CysAsp: 0.3 ± 0.176
0.2CysGlu: 0.2 ± 0.149
0.1CysPhe: 0.1 ± 0.1
0.4CysGly: 0.4 ± 0.167
0.0CysHis: 0.0 ± 0.0
0.2CysIle: 0.2 ± 0.139
0.5CysLys: 0.5 ± 0.205
0.1CysLeu: 0.1 ± 0.089
0.3CysMet: 0.3 ± 0.17
0.5CysAsn: 0.5 ± 0.266
0.2CysPro: 0.2 ± 0.136
0.3CysGln: 0.3 ± 0.154
0.6CysArg: 0.6 ± 0.243
0.2CysSer: 0.2 ± 0.147
0.2CysThr: 0.2 ± 0.153
0.4CysVal: 0.4 ± 0.203
0.2CysTrp: 0.2 ± 0.15
0.3CysTyr: 0.3 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
5.097AspAla: 5.097 ± 0.704
0.2AspCys: 0.2 ± 0.132
6.597AspAsp: 6.597 ± 1.044
5.697AspGlu: 5.697 ± 0.726
3.198AspPhe: 3.198 ± 0.527
4.598AspGly: 4.598 ± 0.89
1.399AspHis: 1.399 ± 0.358
3.698AspIle: 3.698 ± 0.561
6.197AspLys: 6.197 ± 0.999
5.997AspLeu: 5.997 ± 0.67
1.799AspMet: 1.799 ± 0.405
3.498AspAsn: 3.498 ± 0.668
2.799AspPro: 2.799 ± 0.531
1.799AspGln: 1.799 ± 0.419
2.899AspArg: 2.899 ± 0.538
4.898AspSer: 4.898 ± 0.623
2.899AspThr: 2.899 ± 0.615
3.498AspVal: 3.498 ± 0.567
0.7AspTrp: 0.7 ± 0.231
2.399AspTyr: 2.399 ± 0.653
0.0AspXaa: 0.0 ± 0.0
Glu
6.397GluAla: 6.397 ± 0.915
0.1GluCys: 0.1 ± 0.103
4.198GluAsp: 4.198 ± 0.736
4.498GluGlu: 4.498 ± 0.856
1.799GluPhe: 1.799 ± 0.365
3.198GluGly: 3.198 ± 0.577
0.9GluHis: 0.9 ± 0.38
4.098GluIle: 4.098 ± 0.515
2.299GluLys: 2.299 ± 0.508
5.497GluLeu: 5.497 ± 0.489
1.299GluMet: 1.299 ± 0.325
2.699GluAsn: 2.699 ± 0.386
1.299GluPro: 1.299 ± 0.354
4.298GluGln: 4.298 ± 0.644
3.198GluArg: 3.198 ± 0.716
1.399GluSer: 1.399 ± 0.369
2.799GluThr: 2.799 ± 0.538
4.398GluVal: 4.398 ± 0.621
0.6GluTrp: 0.6 ± 0.238
2.999GluTyr: 2.999 ± 0.507
0.0GluXaa: 0.0 ± 0.0
Phe
2.499PheAla: 2.499 ± 0.437
0.2PheCys: 0.2 ± 0.118
2.599PheAsp: 2.599 ± 0.417
1.199PheGlu: 1.199 ± 0.338
1.499PhePhe: 1.499 ± 0.407
2.899PheGly: 2.899 ± 0.54
0.4PheHis: 0.4 ± 0.173
2.499PheIle: 2.499 ± 0.404
2.799PheLys: 2.799 ± 0.545
2.099PheLeu: 2.099 ± 0.421
1.299PheMet: 1.299 ± 0.297
1.999PheAsn: 1.999 ± 0.496
1.199PhePro: 1.199 ± 0.332
1.899PheGln: 1.899 ± 0.43
1.099PheArg: 1.099 ± 0.361
2.999PheSer: 2.999 ± 0.522
3.298PheThr: 3.298 ± 0.459
3.398PheVal: 3.398 ± 0.525
0.3PheTrp: 0.3 ± 0.198
1.699PheTyr: 1.699 ± 0.311
0.0PheXaa: 0.0 ± 0.0
Gly
5.197GlyAla: 5.197 ± 1.142
0.1GlyCys: 0.1 ± 0.092
4.898GlyAsp: 4.898 ± 0.668
3.998GlyGlu: 3.998 ± 0.638
3.098GlyPhe: 3.098 ± 0.599
4.998GlyGly: 4.998 ± 1.011
0.6GlyHis: 0.6 ± 0.235
3.698GlyIle: 3.698 ± 0.957
4.698GlyLys: 4.698 ± 0.596
6.297GlyLeu: 6.297 ± 0.757
3.698GlyMet: 3.698 ± 0.673
4.298GlyAsn: 4.298 ± 0.658
1.199GlyPro: 1.199 ± 0.335
3.098GlyGln: 3.098 ± 0.495
2.599GlyArg: 2.599 ± 0.545
4.598GlySer: 4.598 ± 0.6
3.798GlyThr: 3.798 ± 0.742
5.897GlyVal: 5.897 ± 0.812
1.199GlyTrp: 1.199 ± 0.353
2.799GlyTyr: 2.799 ± 0.5
0.0GlyXaa: 0.0 ± 0.0
His
0.7HisAla: 0.7 ± 0.233
0.2HisCys: 0.2 ± 0.149
1.099HisAsp: 1.099 ± 0.373
0.7HisGlu: 0.7 ± 0.231
0.5HisPhe: 0.5 ± 0.187
1.199HisGly: 1.199 ± 0.325
0.2HisHis: 0.2 ± 0.199
0.8HisIle: 0.8 ± 0.333
0.3HisLys: 0.3 ± 0.237
1.0HisLeu: 1.0 ± 0.354
0.3HisMet: 0.3 ± 0.194
0.5HisAsn: 0.5 ± 0.198
0.5HisPro: 0.5 ± 0.195
0.9HisGln: 0.9 ± 0.304
0.8HisArg: 0.8 ± 0.321
0.6HisSer: 0.6 ± 0.227
0.3HisThr: 0.3 ± 0.165
0.8HisVal: 0.8 ± 0.311
0.2HisTrp: 0.2 ± 0.137
0.6HisTyr: 0.6 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
5.197IleAla: 5.197 ± 0.811
0.4IleCys: 0.4 ± 0.195
5.497IleAsp: 5.497 ± 0.912
3.898IleGlu: 3.898 ± 0.627
1.199IlePhe: 1.199 ± 0.311
3.698IleGly: 3.698 ± 0.64
0.8IleHis: 0.8 ± 0.234
3.098IleIle: 3.098 ± 0.466
5.697IleLys: 5.697 ± 0.822
3.298IleLeu: 3.298 ± 0.611
1.299IleMet: 1.299 ± 0.394
4.698IleAsn: 4.698 ± 0.786
1.999IlePro: 1.999 ± 0.426
3.098IleGln: 3.098 ± 0.625
1.799IleArg: 1.799 ± 0.353
3.098IleSer: 3.098 ± 0.608
3.798IleThr: 3.798 ± 0.564
3.398IleVal: 3.398 ± 0.549
0.8IleTrp: 0.8 ± 0.577
1.699IleTyr: 1.699 ± 0.494
0.0IleXaa: 0.0 ± 0.0
Lys
6.997LysAla: 6.997 ± 0.773
0.3LysCys: 0.3 ± 0.153
4.598LysAsp: 4.598 ± 0.968
3.498LysGlu: 3.498 ± 0.848
2.099LysPhe: 2.099 ± 0.611
3.798LysGly: 3.798 ± 0.492
1.199LysHis: 1.199 ± 0.374
4.098LysIle: 4.098 ± 0.645
4.398LysLys: 4.398 ± 0.972
5.197LysLeu: 5.197 ± 0.851
1.899LysMet: 1.899 ± 0.364
3.898LysAsn: 3.898 ± 0.556
2.799LysPro: 2.799 ± 0.534
3.498LysGln: 3.498 ± 0.538
3.498LysArg: 3.498 ± 0.5
3.798LysSer: 3.798 ± 0.623
4.698LysThr: 4.698 ± 0.56
4.998LysVal: 4.998 ± 0.867
0.9LysTrp: 0.9 ± 0.268
1.899LysTyr: 1.899 ± 0.476
0.0LysXaa: 0.0 ± 0.0
Leu
5.197LeuAla: 5.197 ± 0.795
0.1LeuCys: 0.1 ± 0.089
5.997LeuAsp: 5.997 ± 0.697
5.897LeuGlu: 5.897 ± 1.048
2.599LeuPhe: 2.599 ± 0.5
4.998LeuGly: 4.998 ± 0.604
0.7LeuHis: 0.7 ± 0.319
4.598LeuIle: 4.598 ± 0.641
7.796LeuLys: 7.796 ± 0.871
5.297LeuLeu: 5.297 ± 0.92
1.799LeuMet: 1.799 ± 0.379
4.798LeuAsn: 4.798 ± 0.615
3.698LeuPro: 3.698 ± 0.78
2.799LeuGln: 2.799 ± 0.491
4.498LeuArg: 4.498 ± 0.857
5.097LeuSer: 5.097 ± 0.681
5.797LeuThr: 5.797 ± 0.767
4.798LeuVal: 4.798 ± 0.728
1.199LeuTrp: 1.199 ± 0.379
1.799LeuTyr: 1.799 ± 0.352
0.0LeuXaa: 0.0 ± 0.0
Met
3.098MetAla: 3.098 ± 0.519
0.1MetCys: 0.1 ± 0.101
1.299MetAsp: 1.299 ± 0.316
1.0MetGlu: 1.0 ± 0.339
1.099MetPhe: 1.099 ± 0.214
1.999MetGly: 1.999 ± 0.533
0.3MetHis: 0.3 ± 0.2
1.0MetIle: 1.0 ± 0.29
1.799MetLys: 1.799 ± 0.426
2.599MetLeu: 2.599 ± 0.416
0.2MetMet: 0.2 ± 0.123
1.799MetAsn: 1.799 ± 0.34
0.5MetPro: 0.5 ± 0.262
1.599MetGln: 1.599 ± 0.334
0.9MetArg: 0.9 ± 0.355
1.799MetSer: 1.799 ± 0.423
2.599MetThr: 2.599 ± 0.655
2.499MetVal: 2.499 ± 0.702
0.0MetTrp: 0.0 ± 0.0
0.3MetTyr: 0.3 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
4.798AsnAla: 4.798 ± 0.771
0.4AsnCys: 0.4 ± 0.184
2.899AsnAsp: 2.899 ± 0.678
2.499AsnGlu: 2.499 ± 0.516
2.299AsnPhe: 2.299 ± 0.342
5.097AsnGly: 5.097 ± 0.662
1.399AsnHis: 1.399 ± 0.33
2.799AsnIle: 2.799 ± 0.464
3.198AsnLys: 3.198 ± 0.596
5.197AsnLeu: 5.197 ± 0.715
1.099AsnMet: 1.099 ± 0.369
1.899AsnAsn: 1.899 ± 0.394
2.999AsnPro: 2.999 ± 0.564
2.099AsnGln: 2.099 ± 0.4
2.699AsnArg: 2.699 ± 0.565
3.498AsnSer: 3.498 ± 0.543
3.398AsnThr: 3.398 ± 0.639
3.898AsnVal: 3.898 ± 0.53
1.0AsnTrp: 1.0 ± 0.267
1.599AsnTyr: 1.599 ± 0.369
0.0AsnXaa: 0.0 ± 0.0
Pro
3.098ProAla: 3.098 ± 0.569
0.1ProCys: 0.1 ± 0.098
2.699ProAsp: 2.699 ± 0.764
1.899ProGlu: 1.899 ± 0.42
1.199ProPhe: 1.199 ± 0.358
1.899ProGly: 1.899 ± 0.436
0.1ProHis: 0.1 ± 0.098
1.299ProIle: 1.299 ± 0.421
2.699ProLys: 2.699 ± 0.565
2.099ProLeu: 2.099 ± 0.515
0.5ProMet: 0.5 ± 0.202
1.999ProAsn: 1.999 ± 0.455
1.299ProPro: 1.299 ± 0.35
1.599ProGln: 1.599 ± 0.311
2.199ProArg: 2.199 ± 0.344
2.599ProSer: 2.599 ± 0.629
2.799ProThr: 2.799 ± 0.493
2.599ProVal: 2.599 ± 0.434
0.4ProTrp: 0.4 ± 0.17
1.399ProTyr: 1.399 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
5.497GlnAla: 5.497 ± 0.92
0.3GlnCys: 0.3 ± 0.18
1.999GlnAsp: 1.999 ± 0.439
2.799GlnGlu: 2.799 ± 0.562
1.399GlnPhe: 1.399 ± 0.309
4.198GlnGly: 4.198 ± 0.989
0.1GlnHis: 0.1 ± 0.145
2.799GlnIle: 2.799 ± 0.482
3.798GlnLys: 3.798 ± 0.572
3.498GlnLeu: 3.498 ± 0.625
1.099GlnMet: 1.099 ± 0.314
1.599GlnAsn: 1.599 ± 0.32
1.299GlnPro: 1.299 ± 0.327
2.499GlnGln: 2.499 ± 0.474
1.599GlnArg: 1.599 ± 0.466
3.698GlnSer: 3.698 ± 0.549
3.798GlnThr: 3.798 ± 0.806
3.798GlnVal: 3.798 ± 0.656
0.5GlnTrp: 0.5 ± 0.182
1.199GlnTyr: 1.199 ± 0.282
0.0GlnXaa: 0.0 ± 0.0
Arg
2.799ArgAla: 2.799 ± 0.569
0.3ArgCys: 0.3 ± 0.144
2.099ArgAsp: 2.099 ± 0.459
3.398ArgGlu: 3.398 ± 0.68
2.399ArgPhe: 2.399 ± 0.486
2.899ArgGly: 2.899 ± 0.431
1.0ArgHis: 1.0 ± 0.246
1.999ArgIle: 1.999 ± 0.451
3.198ArgLys: 3.198 ± 0.682
4.598ArgLeu: 4.598 ± 0.854
0.9ArgMet: 0.9 ± 0.286
2.399ArgAsn: 2.399 ± 0.553
1.399ArgPro: 1.399 ± 0.45
1.499ArgGln: 1.499 ± 0.363
3.198ArgArg: 3.198 ± 0.554
1.599ArgSer: 1.599 ± 0.374
2.799ArgThr: 2.799 ± 0.539
3.198ArgVal: 3.198 ± 0.603
0.4ArgTrp: 0.4 ± 0.206
1.899ArgTyr: 1.899 ± 0.41
0.0ArgXaa: 0.0 ± 0.0
Ser
5.097SerAla: 5.097 ± 0.936
0.3SerCys: 0.3 ± 0.153
4.698SerAsp: 4.698 ± 0.595
3.398SerGlu: 3.398 ± 0.428
2.599SerPhe: 2.599 ± 0.381
5.297SerGly: 5.297 ± 0.928
0.2SerHis: 0.2 ± 0.117
4.298SerIle: 4.298 ± 0.649
2.799SerLys: 2.799 ± 0.649
5.597SerLeu: 5.597 ± 0.613
1.599SerMet: 1.599 ± 0.325
2.799SerAsn: 2.799 ± 0.571
2.199SerPro: 2.199 ± 0.464
2.099SerGln: 2.099 ± 0.444
1.899SerArg: 1.899 ± 0.447
3.398SerSer: 3.398 ± 1.055
3.998SerThr: 3.998 ± 0.861
4.398SerVal: 4.398 ± 0.713
0.7SerTrp: 0.7 ± 0.268
2.199SerTyr: 2.199 ± 0.55
0.0SerXaa: 0.0 ± 0.0
Thr
6.697ThrAla: 6.697 ± 1.213
0.7ThrCys: 0.7 ± 0.265
4.398ThrAsp: 4.398 ± 0.828
2.999ThrGlu: 2.999 ± 0.629
1.899ThrPhe: 1.899 ± 0.363
4.898ThrGly: 4.898 ± 0.747
0.5ThrHis: 0.5 ± 0.243
4.698ThrIle: 4.698 ± 0.721
2.999ThrLys: 2.999 ± 0.476
5.997ThrLeu: 5.997 ± 0.773
1.799ThrMet: 1.799 ± 0.448
3.698ThrAsn: 3.698 ± 0.68
3.098ThrPro: 3.098 ± 0.529
2.999ThrGln: 2.999 ± 0.594
2.299ThrArg: 2.299 ± 0.563
3.098ThrSer: 3.098 ± 0.733
4.398ThrThr: 4.398 ± 0.707
6.297ThrVal: 6.297 ± 0.873
0.6ThrTrp: 0.6 ± 0.274
2.399ThrTyr: 2.399 ± 0.51
0.0ThrXaa: 0.0 ± 0.0
Val
5.797ValAla: 5.797 ± 0.783
0.6ValCys: 0.6 ± 0.228
5.197ValAsp: 5.197 ± 0.847
4.198ValGlu: 4.198 ± 0.56
2.899ValPhe: 2.899 ± 0.58
4.898ValGly: 4.898 ± 0.869
0.9ValHis: 0.9 ± 0.262
3.998ValIle: 3.998 ± 0.665
4.798ValLys: 4.798 ± 0.834
3.998ValLeu: 3.998 ± 0.617
1.999ValMet: 1.999 ± 0.46
4.298ValAsn: 4.298 ± 0.58
1.699ValPro: 1.699 ± 0.47
3.798ValGln: 3.798 ± 0.704
2.199ValArg: 2.199 ± 0.534
4.698ValSer: 4.698 ± 0.698
6.297ValThr: 6.297 ± 1.003
6.097ValVal: 6.097 ± 0.789
1.699ValTrp: 1.699 ± 0.503
2.399ValTyr: 2.399 ± 0.526
0.0ValXaa: 0.0 ± 0.0
Trp
0.6TrpAla: 0.6 ± 0.213
0.1TrpCys: 0.1 ± 0.095
0.5TrpAsp: 0.5 ± 0.192
1.0TrpGlu: 1.0 ± 0.324
0.5TrpPhe: 0.5 ± 0.241
0.9TrpGly: 0.9 ± 0.408
0.1TrpHis: 0.1 ± 0.078
0.6TrpIle: 0.6 ± 0.232
0.7TrpLys: 0.7 ± 0.261
1.499TrpLeu: 1.499 ± 0.311
0.0TrpMet: 0.0 ± 0.0
1.499TrpAsn: 1.499 ± 0.543
0.1TrpPro: 0.1 ± 0.089
1.199TrpGln: 1.199 ± 0.503
0.4TrpArg: 0.4 ± 0.162
1.499TrpSer: 1.499 ± 0.448
1.099TrpThr: 1.099 ± 0.35
0.6TrpVal: 0.6 ± 0.234
0.0TrpTrp: 0.0 ± 0.0
0.6TrpTyr: 0.6 ± 0.231
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.298TyrAla: 3.298 ± 0.4
0.5TyrCys: 0.5 ± 0.193
2.399TyrAsp: 2.399 ± 0.562
1.0TyrGlu: 1.0 ± 0.392
1.499TyrPhe: 1.499 ± 0.372
2.399TyrGly: 2.399 ± 0.473
0.6TyrHis: 0.6 ± 0.274
1.699TyrIle: 1.699 ± 0.455
2.599TyrLys: 2.599 ± 0.548
2.699TyrLeu: 2.699 ± 0.478
0.7TyrMet: 0.7 ± 0.214
1.799TyrAsn: 1.799 ± 0.402
0.7TyrPro: 0.7 ± 0.271
2.899TyrGln: 2.899 ± 0.58
2.299TyrArg: 2.299 ± 0.664
2.299TyrSer: 2.299 ± 0.428
1.199TyrThr: 1.199 ± 0.437
2.399TyrVal: 2.399 ± 0.605
0.4TyrTrp: 0.4 ± 0.202
1.399TyrTyr: 1.399 ± 0.36
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10006 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski