Streptomyces sp. NRRL WC-3618

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7314 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N0SY61|A0A0N0SY61_9ACTN HATPase_c domain-containing protein OS=Streptomyces sp. NRRL WC-3618 OX=1519490 GN=ADL01_32270 PE=4 SV=1
MM1 pKa = 7.32TAPTDD6 pKa = 3.7STSALLPTPAAAEE19 pKa = 3.98PPPLSFNVPEE29 pKa = 4.74GFFALPLAATPEE41 pKa = 4.04EE42 pKa = 4.17RR43 pKa = 11.84AEE45 pKa = 3.92RR46 pKa = 11.84AQTFVRR52 pKa = 11.84ALYY55 pKa = 10.73SGGDD59 pKa = 3.49DD60 pKa = 4.63TIWEE64 pKa = 4.17PAAPYY69 pKa = 9.31YY70 pKa = 10.76AAIAEE75 pKa = 4.31LMAGTGVSYY84 pKa = 11.01AAMGLFSTADD94 pKa = 3.5DD95 pKa = 4.51EE96 pKa = 5.58GDD98 pKa = 3.57GDD100 pKa = 3.89EE101 pKa = 4.8SGDD104 pKa = 3.72VARR107 pKa = 11.84HH108 pKa = 5.2SLSDD112 pKa = 3.56GAAQCAFTVAAIPTDD127 pKa = 3.52QAAADD132 pKa = 4.04TDD134 pKa = 3.83IVAQGILATLSRR146 pKa = 11.84DD147 pKa = 3.61PYY149 pKa = 11.62NDD151 pKa = 3.59ARR153 pKa = 11.84WLDD156 pKa = 4.34LPCGPAVFCVTMRR169 pKa = 11.84KK170 pKa = 9.48LKK172 pKa = 10.66LSPDD176 pKa = 3.3VTASGEE182 pKa = 4.24ATDD185 pKa = 5.34LLAGQIQVHH194 pKa = 5.55VPFPTGPFTAIFTLYY209 pKa = 9.45TASTEE214 pKa = 4.09YY215 pKa = 9.86WAEE218 pKa = 3.93FCDD221 pKa = 3.95MMAAILQTVSFAEE234 pKa = 4.22PLAGTDD240 pKa = 3.87ALPVIAGG247 pKa = 3.6

Molecular weight: 25.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8V8A4|A0A0M8V8A4_9ACTN Membrane protein OS=Streptomyces sp. NRRL WC-3618 OX=1519490 GN=ADL01_32795 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7314

0

7314

2386731

21

6357

326.3

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.209 ± 0.043

0.793 ± 0.008

6.056 ± 0.023

5.647 ± 0.029

2.77 ± 0.016

9.382 ± 0.027

2.301 ± 0.012

3.182 ± 0.018

2.274 ± 0.024

10.218 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.728 ± 0.012

1.932 ± 0.017

5.94 ± 0.026

2.869 ± 0.018

7.807 ± 0.03

5.278 ± 0.023

6.348 ± 0.023

8.517 ± 0.027

1.576 ± 0.013

2.17 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski