Amino acid dipepetide frequency for Streptomyces sp. NRRL WC-3618

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.745AlaAla: 19.745 ± 0.141	1.018AlaCys: 1.018 ± 0.024	8.366AlaAsp: 8.366 ± 0.07	8.513AlaGlu: 8.513 ± 0.088	3.437AlaPhe: 3.437 ± 0.042	12.487AlaGly: 12.487 ± 0.086	2.852AlaHis: 2.852 ± 0.035	3.458AlaIle: 3.458 ± 0.041	2.998AlaLys: 2.998 ± 0.048	13.999AlaLeu: 13.999 ± 0.102	2.405AlaMet: 2.405 ± 0.031	2.114AlaAsn: 2.114 ± 0.03	6.879AlaPro: 6.879 ± 0.074	3.938AlaGln: 3.938 ± 0.049	9.861AlaArg: 9.861 ± 0.082	6.08AlaSer: 6.08 ± 0.06	7.322AlaThr: 7.322 ± 0.062	11.958AlaVal: 11.958 ± 0.084	1.857AlaTrp: 1.857 ± 0.028	2.806AlaTyr: 2.806 ± 0.037	0.0AlaXaa: 0.0 ± 0.0
Cys	1.072CysAla: 1.072 ± 0.022	0.096CysCys: 0.096 ± 0.006	0.481CysAsp: 0.481 ± 0.016	0.426CysGlu: 0.426 ± 0.015	0.224CysPhe: 0.224 ± 0.01	0.946CysGly: 0.946 ± 0.024	0.189CysHis: 0.189 ± 0.008	0.15CysIle: 0.15 ± 0.009	0.121CysLys: 0.121 ± 0.007	0.804CysLeu: 0.804 ± 0.02	0.122CysMet: 0.122 ± 0.006	0.14CysAsn: 0.14 ± 0.008	0.459CysPro: 0.459 ± 0.014	0.179CysGln: 0.179 ± 0.009	0.574CysArg: 0.574 ± 0.018	0.434CysSer: 0.434 ± 0.012	0.514CysThr: 0.514 ± 0.015	0.717CysVal: 0.717 ± 0.018	0.135CysTrp: 0.135 ± 0.007	0.152CysTyr: 0.152 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.603AspAla: 7.603 ± 0.071	0.455AspCys: 0.455 ± 0.013	3.713AspAsp: 3.713 ± 0.043	3.858AspGlu: 3.858 ± 0.041	1.743AspPhe: 1.743 ± 0.027	6.325AspGly: 6.325 ± 0.057	1.447AspHis: 1.447 ± 0.023	2.032AspIle: 2.032 ± 0.032	1.252AspLys: 1.252 ± 0.027	6.198AspLeu: 6.198 ± 0.05	0.846AspMet: 0.846 ± 0.02	1.096AspAsn: 1.096 ± 0.023	4.415AspPro: 4.415 ± 0.051	1.626AspGln: 1.626 ± 0.025	4.719AspArg: 4.719 ± 0.048	2.817AspSer: 2.817 ± 0.035	3.36AspThr: 3.36 ± 0.038	4.831AspVal: 4.831 ± 0.043	1.076AspTrp: 1.076 ± 0.025	1.153AspTyr: 1.153 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	7.248GluAla: 7.248 ± 0.071	0.377GluCys: 0.377 ± 0.013	2.715GluAsp: 2.715 ± 0.04	3.429GluGlu: 3.429 ± 0.048	1.537GluPhe: 1.537 ± 0.025	4.35GluGly: 4.35 ± 0.049	1.51GluHis: 1.51 ± 0.027	2.238GluIle: 2.238 ± 0.036	1.455GluLys: 1.455 ± 0.028	6.856GluLeu: 6.856 ± 0.073	0.874GluMet: 0.874 ± 0.021	1.068GluAsn: 1.068 ± 0.02	3.215GluPro: 3.215 ± 0.046	2.249GluGln: 2.249 ± 0.036	5.231GluArg: 5.231 ± 0.051	2.738GluSer: 2.738 ± 0.038	2.937GluThr: 2.937 ± 0.038	4.461GluVal: 4.461 ± 0.047	0.813GluTrp: 0.813 ± 0.02	1.169GluTyr: 1.169 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	3.617PheAla: 3.617 ± 0.041	0.273PheCys: 0.273 ± 0.01	2.004PheAsp: 2.004 ± 0.029	1.469PheGlu: 1.469 ± 0.027	0.879PhePhe: 0.879 ± 0.022	3.015PheGly: 3.015 ± 0.038	0.64PheHis: 0.64 ± 0.016	0.727PheIle: 0.727 ± 0.018	0.553PheLys: 0.553 ± 0.016	2.544PheLeu: 2.544 ± 0.036	0.424PheMet: 0.424 ± 0.013	0.602PheAsn: 0.602 ± 0.017	1.368PhePro: 1.368 ± 0.026	0.714PheGln: 0.714 ± 0.016	1.89PheArg: 1.89 ± 0.027	1.541PheSer: 1.541 ± 0.029	2.086PheThr: 2.086 ± 0.029	2.304PheVal: 2.304 ± 0.031	0.442PheTrp: 0.442 ± 0.013	0.608PheTyr: 0.608 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	10.949GlyAla: 10.949 ± 0.085	0.823GlyCys: 0.823 ± 0.019	5.195GlyAsp: 5.195 ± 0.05	5.02GlyGlu: 5.02 ± 0.045	2.914GlyPhe: 2.914 ± 0.032	8.765GlyGly: 8.765 ± 0.085	2.36GlyHis: 2.36 ± 0.038	3.419GlyIle: 3.419 ± 0.042	2.566GlyLys: 2.566 ± 0.039	9.21GlyLeu: 9.21 ± 0.068	1.925GlyMet: 1.925 ± 0.028	1.947GlyAsn: 1.947 ± 0.039	4.885GlyPro: 4.885 ± 0.052	2.781GlyGln: 2.781 ± 0.045	7.379GlyArg: 7.379 ± 0.06	5.461GlySer: 5.461 ± 0.058	6.538GlyThr: 6.538 ± 0.063	7.697GlyVal: 7.697 ± 0.063	1.72GlyTrp: 1.72 ± 0.027	2.266GlyTyr: 2.266 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.646HisAla: 2.646 ± 0.031	0.222HisCys: 0.222 ± 0.01	1.358HisAsp: 1.358 ± 0.024	1.232HisGlu: 1.232 ± 0.025	0.649HisPhe: 0.649 ± 0.017	2.389HisGly: 2.389 ± 0.034	0.688HisHis: 0.688 ± 0.017	0.689HisIle: 0.689 ± 0.019	0.393HisLys: 0.393 ± 0.013	2.436HisLeu: 2.436 ± 0.037	0.342HisMet: 0.342 ± 0.012	0.412HisAsn: 0.412 ± 0.013	1.804HisPro: 1.804 ± 0.029	0.662HisGln: 0.662 ± 0.017	2.019HisArg: 2.019 ± 0.033	1.059HisSer: 1.059 ± 0.022	1.427HisThr: 1.427 ± 0.027	1.683HisVal: 1.683 ± 0.03	0.385HisTrp: 0.385 ± 0.012	0.514HisTyr: 0.514 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.597IleAla: 4.597 ± 0.047	0.278IleCys: 0.278 ± 0.011	2.306IleAsp: 2.306 ± 0.035	1.985IleGlu: 1.985 ± 0.029	0.706IlePhe: 0.706 ± 0.019	3.468IleGly: 3.468 ± 0.044	0.617IleHis: 0.617 ± 0.017	0.888IleIle: 0.888 ± 0.022	0.776IleLys: 0.776 ± 0.024	2.386IleLeu: 2.386 ± 0.037	0.466IleMet: 0.466 ± 0.017	0.75IleAsn: 0.75 ± 0.024	1.822IlePro: 1.822 ± 0.029	0.768IleGln: 0.768 ± 0.018	2.307IleArg: 2.307 ± 0.028	1.71IleSer: 1.71 ± 0.026	2.297IleThr: 2.297 ± 0.032	2.746IleVal: 2.746 ± 0.04	0.395IleTrp: 0.395 ± 0.013	0.553IleTyr: 0.553 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	3.039LysAla: 3.039 ± 0.044	0.141LysCys: 0.141 ± 0.009	1.412LysAsp: 1.412 ± 0.029	1.227LysGlu: 1.227 ± 0.031	0.494LysPhe: 0.494 ± 0.017	2.021LysGly: 2.021 ± 0.035	0.416LysHis: 0.416 ± 0.014	0.934LysIle: 0.934 ± 0.02	0.873LysLys: 0.873 ± 0.027	2.17LysLeu: 2.17 ± 0.036	0.405LysMet: 0.405 ± 0.014	0.574LysAsn: 0.574 ± 0.019	1.39LysPro: 1.39 ± 0.029	0.75LysGln: 0.75 ± 0.023	1.436LysArg: 1.436 ± 0.027	1.283LysSer: 1.283 ± 0.027	1.35LysThr: 1.35 ± 0.03	2.029LysVal: 2.029 ± 0.037	0.294LysTrp: 0.294 ± 0.011	0.505LysTyr: 0.505 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.63LeuAla: 14.63 ± 0.101	0.848LeuCys: 0.848 ± 0.02	6.666LeuAsp: 6.666 ± 0.056	4.663LeuGlu: 4.663 ± 0.053	2.544LeuPhe: 2.544 ± 0.036	9.09LeuGly: 9.09 ± 0.071	2.246LeuHis: 2.246 ± 0.034	3.236LeuIle: 3.236 ± 0.041	2.172LeuLys: 2.172 ± 0.035	11.028LeuLeu: 11.028 ± 0.101	1.612LeuMet: 1.612 ± 0.025	1.807LeuAsn: 1.807 ± 0.03	6.413LeuPro: 6.413 ± 0.055	2.18LeuGln: 2.18 ± 0.036	8.503LeuArg: 8.503 ± 0.083	5.379LeuSer: 5.379 ± 0.049	7.186LeuThr: 7.186 ± 0.067	8.681LeuVal: 8.681 ± 0.072	1.365LeuTrp: 1.365 ± 0.027	1.928LeuTyr: 1.928 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.217MetAla: 2.217 ± 0.032	0.145MetCys: 0.145 ± 0.007	0.921MetAsp: 0.921 ± 0.021	0.791MetGlu: 0.791 ± 0.02	0.466MetPhe: 0.466 ± 0.015	1.329MetGly: 1.329 ± 0.027	0.365MetHis: 0.365 ± 0.013	0.654MetIle: 0.654 ± 0.017	0.428MetLys: 0.428 ± 0.015	1.628MetLeu: 1.628 ± 0.03	0.282MetMet: 0.282 ± 0.012	0.415MetAsn: 0.415 ± 0.013	1.096MetPro: 1.096 ± 0.024	0.415MetGln: 0.415 ± 0.015	1.451MetArg: 1.451 ± 0.026	1.307MetSer: 1.307 ± 0.021	1.474MetThr: 1.474 ± 0.024	1.345MetVal: 1.345 ± 0.025	0.228MetTrp: 0.228 ± 0.009	0.323MetTyr: 0.323 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.307AsnAla: 2.307 ± 0.035	0.189AsnCys: 0.189 ± 0.01	1.034AsnAsp: 1.034 ± 0.022	0.853AsnGlu: 0.853 ± 0.017	0.546AsnPhe: 0.546 ± 0.017	2.105AsnGly: 2.105 ± 0.037	0.45AsnHis: 0.45 ± 0.015	0.697AsnIle: 0.697 ± 0.017	0.468AsnLys: 0.468 ± 0.014	1.743AsnLeu: 1.743 ± 0.031	0.323AsnMet: 0.323 ± 0.012	0.516AsnAsn: 0.516 ± 0.019	1.42AsnPro: 1.42 ± 0.024	0.605AsnGln: 0.605 ± 0.017	1.365AsnArg: 1.365 ± 0.022	1.117AsnSer: 1.117 ± 0.025	1.243AsnThr: 1.243 ± 0.028	1.528AsnVal: 1.528 ± 0.025	0.355AsnTrp: 0.355 ± 0.014	0.46AsnTyr: 0.46 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.088ProAla: 8.088 ± 0.069	0.325ProCys: 0.325 ± 0.012	4.449ProAsp: 4.449 ± 0.051	4.196ProGlu: 4.196 ± 0.049	1.535ProPhe: 1.535 ± 0.026	6.322ProGly: 6.322 ± 0.061	1.384ProHis: 1.384 ± 0.026	1.287ProIle: 1.287 ± 0.022	1.237ProLys: 1.237 ± 0.025	5.306ProLeu: 5.306 ± 0.051	0.98ProMet: 0.98 ± 0.023	0.984ProAsn: 0.984 ± 0.02	3.296ProPro: 3.296 ± 0.05	1.776ProGln: 1.776 ± 0.033	3.633ProArg: 3.633 ± 0.045	3.266ProSer: 3.266 ± 0.046	3.454ProThr: 3.454 ± 0.044	5.502ProVal: 5.502 ± 0.06	0.939ProTrp: 0.939 ± 0.022	1.445ProTyr: 1.445 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.779GlnAla: 3.779 ± 0.045	0.196GlnCys: 0.196 ± 0.009	1.474GlnAsp: 1.474 ± 0.025	1.48GlnGlu: 1.48 ± 0.032	0.765GlnPhe: 0.765 ± 0.019	2.394GlnGly: 2.394 ± 0.036	0.702GlnHis: 0.702 ± 0.018	1.101GlnIle: 1.101 ± 0.022	0.641GlnLys: 0.641 ± 0.019	3.235GlnLeu: 3.235 ± 0.04	0.498GlnMet: 0.498 ± 0.016	0.568GlnAsn: 0.568 ± 0.018	1.681GlnPro: 1.681 ± 0.031	1.324GlnGln: 1.324 ± 0.036	2.41GlnArg: 2.41 ± 0.037	1.374GlnSer: 1.374 ± 0.026	1.445GlnThr: 1.445 ± 0.027	2.441GlnVal: 2.441 ± 0.034	0.505GlnTrp: 0.505 ± 0.018	0.677GlnTyr: 0.677 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.542ArgAla: 9.542 ± 0.084	0.581ArgCys: 0.581 ± 0.018	4.197ArgAsp: 4.197 ± 0.047	4.711ArgGlu: 4.711 ± 0.054	2.286ArgPhe: 2.286 ± 0.033	5.568ArgGly: 5.568 ± 0.045	2.062ArgHis: 2.062 ± 0.03	3.076ArgIle: 3.076 ± 0.036	1.666ArgLys: 1.666 ± 0.03	8.624ArgLeu: 8.624 ± 0.084	1.675ArgMet: 1.675 ± 0.029	1.409ArgAsn: 1.409 ± 0.026	4.669ArgPro: 4.669 ± 0.049	2.36ArgGln: 2.36 ± 0.036	7.263ArgArg: 7.263 ± 0.074	3.993ArgSer: 3.993 ± 0.05	5.376ArgThr: 5.376 ± 0.064	5.89ArgVal: 5.89 ± 0.053	1.357ArgTrp: 1.357 ± 0.024	1.769ArgTyr: 1.769 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.911SerAla: 6.911 ± 0.061	0.427SerCys: 0.427 ± 0.016	2.913SerAsp: 2.913 ± 0.036	2.459SerGlu: 2.459 ± 0.033	1.613SerPhe: 1.613 ± 0.028	6.174SerGly: 6.174 ± 0.065	1.071SerHis: 1.071 ± 0.023	1.428SerIle: 1.428 ± 0.022	1.131SerLys: 1.131 ± 0.025	5.016SerLeu: 5.016 ± 0.05	1.102SerMet: 1.102 ± 0.021	0.997SerAsn: 0.997 ± 0.025	3.309SerPro: 3.309 ± 0.043	1.383SerGln: 1.383 ± 0.026	3.667SerArg: 3.667 ± 0.043	3.074SerSer: 3.074 ± 0.043	3.302SerThr: 3.302 ± 0.049	4.49SerVal: 4.49 ± 0.044	0.957SerTrp: 0.957 ± 0.023	1.359SerTyr: 1.359 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	8.933ThrAla: 8.933 ± 0.074	0.443ThrCys: 0.443 ± 0.014	3.817ThrAsp: 3.817 ± 0.036	3.312ThrGlu: 3.312 ± 0.04	1.726ThrPhe: 1.726 ± 0.033	6.981ThrGly: 6.981 ± 0.072	1.297ThrHis: 1.297 ± 0.022	1.787ThrIle: 1.787 ± 0.032	1.283ThrLys: 1.283 ± 0.026	5.819ThrLeu: 5.819 ± 0.047	0.99ThrMet: 0.99 ± 0.021	1.181ThrAsn: 1.181 ± 0.026	4.254ThrPro: 4.254 ± 0.047	1.473ThrGln: 1.473 ± 0.024	3.951ThrArg: 3.951 ± 0.043	3.492ThrSer: 3.492 ± 0.042	4.049ThrThr: 4.049 ± 0.058	6.227ThrVal: 6.227 ± 0.058	0.994ThrTrp: 0.994 ± 0.022	1.476ThrTyr: 1.476 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.641ValAla: 10.641 ± 0.074	0.763ValCys: 0.763 ± 0.02	5.064ValAsp: 5.064 ± 0.046	4.751ValGlu: 4.751 ± 0.052	2.446ValPhe: 2.446 ± 0.035	6.583ValGly: 6.583 ± 0.056	1.936ValHis: 1.936 ± 0.027	2.925ValIle: 2.925 ± 0.036	1.869ValLys: 1.869 ± 0.033	9.292ValLeu: 9.292 ± 0.071	1.453ValMet: 1.453 ± 0.026	1.815ValAsn: 1.815 ± 0.033	5.159ValPro: 5.159 ± 0.052	2.184ValGln: 2.184 ± 0.035	7.272ValArg: 7.272 ± 0.069	4.548ValSer: 4.548 ± 0.048	5.755ValThr: 5.755 ± 0.049	7.887ValVal: 7.887 ± 0.069	1.185ValTrp: 1.185 ± 0.027	1.639ValTyr: 1.639 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.691TrpAla: 1.691 ± 0.029	0.155TrpCys: 0.155 ± 0.008	0.899TrpAsp: 0.899 ± 0.023	0.754TrpGlu: 0.754 ± 0.019	0.514TrpPhe: 0.514 ± 0.015	1.11TrpGly: 1.11 ± 0.025	0.39TrpHis: 0.39 ± 0.014	0.582TrpIle: 0.582 ± 0.015	0.41TrpLys: 0.41 ± 0.014	1.784TrpLeu: 1.784 ± 0.031	0.276TrpMet: 0.276 ± 0.011	0.464TrpAsn: 0.464 ± 0.014	0.782TrpPro: 0.782 ± 0.018	0.668TrpGln: 0.668 ± 0.016	1.338TrpArg: 1.338 ± 0.026	1.044TrpSer: 1.044 ± 0.025	1.143TrpThr: 1.143 ± 0.024	0.985TrpVal: 0.985 ± 0.021	0.36TrpTrp: 0.36 ± 0.013	0.409TrpTyr: 0.409 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.837TyrAla: 2.837 ± 0.038	0.179TyrCys: 0.179 ± 0.009	1.581TyrAsp: 1.581 ± 0.031	1.34TyrGlu: 1.34 ± 0.023	0.68TyrPhe: 0.68 ± 0.018	2.399TyrGly: 2.399 ± 0.036	0.388TyrHis: 0.388 ± 0.014	0.512TyrIle: 0.512 ± 0.015	0.451TyrLys: 0.451 ± 0.015	2.1TyrLeu: 2.1 ± 0.03	0.283TyrMet: 0.283 ± 0.01	0.463TyrAsn: 0.463 ± 0.014	1.089TyrPro: 1.089 ± 0.023	0.656TyrGln: 0.656 ± 0.016	1.802TyrArg: 1.802 ± 0.031	1.066TyrSer: 1.066 ± 0.023	1.226TyrThr: 1.226 ± 0.024	1.764TyrVal: 1.764 ± 0.027	0.395TyrTrp: 0.395 ± 0.014	0.491TyrTyr: 0.491 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7314 proteins (2386731 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---