Aureimonas fodinaquatilis
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3594 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B0DV82|A0A5B0DV82_9RHIZ Winged helix DNA-binding protein OS=Aureimonas fodinaquatilis OX=2565783 GN=FPY71_06875 PE=4 SV=1
MM1 pKa = 7.57 SNRR4 pKa = 11.84 NTVLFGASLLAAIGAVAVDD23 pKa = 4.27 YY24 pKa = 10.97 SVSDD28 pKa = 3.58 APRR31 pKa = 11.84 AVAAQVEE38 pKa = 4.58 DD39 pKa = 4.01 AAPCAAATPSPASGARR55 pKa = 11.84 NYY57 pKa = 11.43 DD58 pKa = 4.39 DD59 pKa = 3.94 IANDD63 pKa = 3.74 YY64 pKa = 11.15 SSGSAPAPAPAPMPAEE80 pKa = 4.19 NDD82 pKa = 3.67 ANPCSAGVLL91 pKa = 3.81
Molecular weight: 8.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.808
IPC_protein 3.694
Toseland 3.49
ProMoST 3.923
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.834
Patrickios 0.604
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A5B0DRB3|A0A5B0DRB3_9RHIZ Sulfurtransferase OS=Aureimonas fodinaquatilis OX=2565783 GN=FPY71_12305 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATTGGRR28 pKa = 11.84 RR29 pKa = 11.84 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3594
0
3594
1177670
25
2166
327.7
35.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.972 ± 0.05
0.785 ± 0.011
5.489 ± 0.033
5.729 ± 0.039
3.938 ± 0.025
8.436 ± 0.04
1.98 ± 0.021
5.545 ± 0.032
2.988 ± 0.027
10.277 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.582 ± 0.018
2.93 ± 0.022
4.947 ± 0.031
3.543 ± 0.025
6.687 ± 0.039
5.883 ± 0.025
5.286 ± 0.027
7.488 ± 0.035
1.221 ± 0.016
2.293 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here