Desulfatibacillum aliphaticivorans
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5198 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B8FAR4|B8FAR4_DESAL Methyltransferase type 12 OS=Desulfatibacillum aliphaticivorans OX=218208 GN=Dalk_1662 PE=4 SV=1
MM1 pKa = 7.63 PCKK4 pKa = 10.43 LYY6 pKa = 9.96 WKK8 pKa = 10.21 SFALSVLLLSFFIAFTGCSDD28 pKa = 4.83 DD29 pKa = 6.22 DD30 pKa = 4.03 DD31 pKa = 4.33 VLYY34 pKa = 11.2 SGATSTGKK42 pKa = 10.4 AVLGPLCGADD52 pKa = 3.32 YY53 pKa = 11.1 KK54 pKa = 11.39 VFAYY58 pKa = 9.85 PDD60 pKa = 3.62 MKK62 pKa = 11.16 NPIITGKK69 pKa = 9.14 TSVSDD74 pKa = 4.26 DD75 pKa = 3.49 LSQAGLFSLPDD86 pKa = 3.46 DD87 pKa = 4.2 RR88 pKa = 11.84 LADD91 pKa = 3.21 GWLYY95 pKa = 10.69 IMEE98 pKa = 4.48 VTGGTDD104 pKa = 2.89 VDD106 pKa = 4.17 ADD108 pKa = 4.0 DD109 pKa = 6.11 DD110 pKa = 4.54 GVIDD114 pKa = 5.44 AAATPNLGTIHH125 pKa = 7.41 LALQGEE131 pKa = 4.75 TIKK134 pKa = 11.11 KK135 pKa = 9.55 EE136 pKa = 3.98 GFRR139 pKa = 11.84 VNILSEE145 pKa = 4.3 LVYY148 pKa = 9.57 QTVSFMLMAGYY159 pKa = 8.29 PQSDD163 pKa = 3.86 VIARR167 pKa = 11.84 MNAAASRR174 pKa = 11.84 LINQDD179 pKa = 2.75 LDD181 pKa = 3.9 GNGQIDD187 pKa = 4.38 HH188 pKa = 7.5 KK189 pKa = 11.21 DD190 pKa = 3.16 LAYY193 pKa = 9.7 WEE195 pKa = 4.41 PVNNRR200 pKa = 11.84 GALVRR205 pKa = 11.84 GWASITPCIDD215 pKa = 3.04 AVHH218 pKa = 6.52 NNDD221 pKa = 4.81 DD222 pKa = 4.17 LFSLAVNRR230 pKa = 11.84 TSPQSALANTPGTALNVAVDD250 pKa = 3.47 GGFAYY255 pKa = 10.62 VADD258 pKa = 4.5 ADD260 pKa = 4.27 GGLHH264 pKa = 6.52 VFDD267 pKa = 4.39 TTDD270 pKa = 3.26 PFDD273 pKa = 4.16 PVLLGTAAMDD283 pKa = 3.94 RR284 pKa = 11.84 ARR286 pKa = 11.84 DD287 pKa = 3.76 VVISGTYY294 pKa = 10.19 AYY296 pKa = 10.61 VADD299 pKa = 4.56 DD300 pKa = 4.2 AEE302 pKa = 4.32 GLKK305 pKa = 10.17 IVDD308 pKa = 4.26 AADD311 pKa = 3.89 PSDD314 pKa = 3.77 PQVVGVVDD322 pKa = 3.7 TEE324 pKa = 3.95 KK325 pKa = 10.99 ARR327 pKa = 11.84 AVVVRR332 pKa = 11.84 GNYY335 pKa = 10.34 AYY337 pKa = 10.89 VADD340 pKa = 4.78 DD341 pKa = 4.03 ANGLRR346 pKa = 11.84 VIDD349 pKa = 4.02 VADD352 pKa = 3.89 PANPQIVGTAPTVKK366 pKa = 10.55 ANDD369 pKa = 3.14 VALFGDD375 pKa = 4.49 YY376 pKa = 11.06 ACVADD381 pKa = 4.78 DD382 pKa = 3.63 VGGVKK387 pKa = 10.17 VVDD390 pKa = 3.55 IADD393 pKa = 3.94 PANPQVVGTAATEE406 pKa = 4.26 DD407 pKa = 3.6 ASSAAIADD415 pKa = 4.56 GYY417 pKa = 11.64 AYY419 pKa = 10.74 VADD422 pKa = 3.74 SAFGIKK428 pKa = 10.03 VVDD431 pKa = 4.78 LSDD434 pKa = 3.24 PTAPVVVGAASTGFSRR450 pKa = 11.84 SMEE453 pKa = 3.97 VFNRR457 pKa = 11.84 VAYY460 pKa = 8.63 VTGDD464 pKa = 3.04 SDD466 pKa = 3.82 GFKK469 pKa = 10.13 MVDD472 pKa = 3.05 VSDD475 pKa = 4.01 PANPKK480 pKa = 10.21 IIGTRR485 pKa = 11.84 DD486 pKa = 3.07 EE487 pKa = 4.63 GDD489 pKa = 3.34 TRR491 pKa = 11.84 GLWIGDD497 pKa = 3.72 NIAYY501 pKa = 8.92 LANGYY506 pKa = 9.67 QGLKK510 pKa = 8.55 VTDD513 pKa = 3.27 ITYY516 pKa = 10.16 EE517 pKa = 3.98 PNPQVIGCVNTADD530 pKa = 3.82 AQDD533 pKa = 3.45 AAFQGDD539 pKa = 4.04 YY540 pKa = 10.97 AYY542 pKa = 11.02 VADD545 pKa = 4.52 GAGGLAVVDD554 pKa = 4.31 ISDD557 pKa = 3.91 PANPVLIGSAPSTFAQGAAVQGDD580 pKa = 4.04 YY581 pKa = 11.05 AYY583 pKa = 11.03 VADD586 pKa = 4.96 GGDD589 pKa = 3.49 GLRR592 pKa = 11.84 IFDD595 pKa = 4.18 VSDD598 pKa = 3.43 PANPFEE604 pKa = 4.25 TSVLDD609 pKa = 3.6 TDD611 pKa = 4.32 DD612 pKa = 4.49 ANIVAVSGDD621 pKa = 3.66 YY622 pKa = 10.82 AYY624 pKa = 11.1 VADD627 pKa = 5.04 GSAGLRR633 pKa = 11.84 VIDD636 pKa = 3.8 VSNPYY641 pKa = 10.22 SPQLKK646 pKa = 8.47 GTLDD650 pKa = 3.17 TRR652 pKa = 11.84 SAYY655 pKa = 10.36 DD656 pKa = 3.33 VTVSGDD662 pKa = 3.22 LAYY665 pKa = 11.06 VGDD668 pKa = 4.5 GMDD671 pKa = 3.53 GLIIVDD677 pKa = 3.68 VSDD680 pKa = 4.64 PEE682 pKa = 4.17 NPDD685 pKa = 4.18 DD686 pKa = 5.58 IGVLDD691 pKa = 4.38 TFFADD696 pKa = 6.05 DD697 pKa = 3.75 IVLDD701 pKa = 3.86 GNYY704 pKa = 10.39 AYY706 pKa = 9.79 IADD709 pKa = 4.57 GPQGLKK715 pKa = 10.31 VADD718 pKa = 4.04 ISDD721 pKa = 3.77 PTAPEE726 pKa = 3.44 QKK728 pKa = 10.59 AYY730 pKa = 11.06 LDD732 pKa = 3.97 TPNSCRR738 pKa = 11.84 ALALSGNYY746 pKa = 9.86 LYY748 pKa = 10.98 AADD751 pKa = 5.11 NGPDD755 pKa = 3.67 MIIVDD760 pKa = 4.12 VSDD763 pKa = 3.9 PLNPVEE769 pKa = 4.23 IAWVEE774 pKa = 4.09 TLNVFYY780 pKa = 10.57 RR781 pKa = 11.84 LEE783 pKa = 3.89 VHH785 pKa = 6.77 EE786 pKa = 5.43 GYY788 pKa = 10.86 LYY790 pKa = 9.77 LTSSEE795 pKa = 5.07 LGLQVLKK802 pKa = 10.81 AVPAPP807 pKa = 3.64
Molecular weight: 84.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.667
IPC2_protein 3.681
IPC_protein 3.757
Toseland 3.503
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.745
Rodwell 3.579
Grimsley 3.414
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.215
Thurlkill 3.567
EMBOSS 3.757
Sillero 3.884
Patrickios 1.57
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|B8FN68|B8FN68_DESAL Ion-translocating oxidoreductase complex subunit A OS=Desulfatibacillum aliphaticivorans OX=218208 GN=rnfA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.24 IKK11 pKa = 10.37 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 6.37 GFLKK20 pKa = 10.63 RR21 pKa = 11.84 MSTKK25 pKa = 10.08 AGRR28 pKa = 11.84 RR29 pKa = 11.84 IIKK32 pKa = 9.74 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.98 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.301
IPC_protein 12.881
Toseland 13.056
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.749
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.486
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.2
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5198
0
5198
1868736
31
15349
359.5
39.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.395 ± 0.05
1.331 ± 0.017
5.866 ± 0.048
6.594 ± 0.048
4.203 ± 0.034
7.969 ± 0.065
1.889 ± 0.017
5.917 ± 0.03
5.644 ± 0.057
9.571 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.97 ± 0.024
3.65 ± 0.033
4.612 ± 0.034
3.417 ± 0.02
4.971 ± 0.043
5.829 ± 0.038
4.941 ± 0.064
7.03 ± 0.037
1.194 ± 0.014
3.007 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here