Thauera chlorobenzoica
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3343 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L6FGW0|A0A1L6FGW0_9RHOO O-succinylhomoserine sulfhydrylase OS=Thauera chlorobenzoica OX=96773 GN=metZ PE=3 SV=1
MM1 pKa = 7.71 LYY3 pKa = 10.66 APDD6 pKa = 4.16 HH7 pKa = 5.68 YY8 pKa = 10.79 RR9 pKa = 11.84 GLDD12 pKa = 3.46 LGEE15 pKa = 5.01 LEE17 pKa = 5.59 LDD19 pKa = 3.95 NACVACALLADD30 pKa = 4.25 YY31 pKa = 8.3 LTGEE35 pKa = 4.73 DD36 pKa = 4.22 EE37 pKa = 4.73 PPTGEE42 pKa = 4.6 LDD44 pKa = 3.53 SLSRR48 pKa = 11.84 QVADD52 pKa = 4.19 SMAALGWSIPPDD64 pKa = 3.66 AAPAFALKK72 pKa = 10.7 VCGDD76 pKa = 3.89 SEE78 pKa = 4.39 RR79 pKa = 11.84 LNN81 pKa = 3.65
Molecular weight: 8.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.897
Patrickios 0.769
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A1L6FHD7|A0A1L6FHD7_9RHOO Putative pilus assembly protein OS=Thauera chlorobenzoica OX=96773 GN=Tchl_3360 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.6 RR14 pKa = 11.84 THH16 pKa = 5.75 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.7 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVIRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.91 GRR39 pKa = 11.84 HH40 pKa = 4.92 RR41 pKa = 11.84 LAVV44 pKa = 3.37
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3343
0
3343
1097188
37
2131
328.2
35.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.471 ± 0.065
1.021 ± 0.015
5.382 ± 0.032
5.938 ± 0.04
3.535 ± 0.025
8.476 ± 0.04
2.402 ± 0.019
4.507 ± 0.029
2.845 ± 0.037
11.147 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.266 ± 0.022
2.291 ± 0.023
5.19 ± 0.029
3.316 ± 0.027
7.812 ± 0.042
4.762 ± 0.027
4.683 ± 0.027
7.423 ± 0.045
1.365 ± 0.018
2.169 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here