Amino acid dipepetide frequency for Thauera chlorobenzoica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.453AlaAla: 20.453 ± 0.24	1.521AlaCys: 1.521 ± 0.046	7.044AlaAsp: 7.044 ± 0.092	8.282AlaGlu: 8.282 ± 0.108	4.3AlaPhe: 4.3 ± 0.057	12.07AlaGly: 12.07 ± 0.129	2.988AlaHis: 2.988 ± 0.06	5.424AlaIle: 5.424 ± 0.078	3.178AlaLys: 3.178 ± 0.072	16.442AlaLeu: 16.442 ± 0.174	3.172AlaMet: 3.172 ± 0.058	2.519AlaAsn: 2.519 ± 0.048	6.418AlaPro: 6.418 ± 0.106	4.751AlaGln: 4.751 ± 0.075	10.929AlaArg: 10.929 ± 0.137	5.845AlaSer: 5.845 ± 0.084	5.703AlaThr: 5.703 ± 0.072	9.461AlaVal: 9.461 ± 0.106	1.84AlaTrp: 1.84 ± 0.047	2.372AlaTyr: 2.372 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	1.291CysAla: 1.291 ± 0.04	0.153CysCys: 0.153 ± 0.013	0.612CysAsp: 0.612 ± 0.028	0.565CysGlu: 0.565 ± 0.025	0.356CysPhe: 0.356 ± 0.017	1.091CysGly: 1.091 ± 0.035	0.308CysHis: 0.308 ± 0.019	0.427CysIle: 0.427 ± 0.019	0.242CysLys: 0.242 ± 0.017	0.926CysLeu: 0.926 ± 0.029	0.183CysMet: 0.183 ± 0.014	0.246CysAsn: 0.246 ± 0.015	0.614CysPro: 0.614 ± 0.028	0.23CysGln: 0.23 ± 0.015	0.81CysArg: 0.81 ± 0.028	0.509CysSer: 0.509 ± 0.024	0.554CysThr: 0.554 ± 0.026	0.709CysVal: 0.709 ± 0.027	0.136CysTrp: 0.136 ± 0.011	0.246CysTyr: 0.246 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	7.245AspAla: 7.245 ± 0.095	0.522AspCys: 0.522 ± 0.022	2.95AspAsp: 2.95 ± 0.062	3.776AspGlu: 3.776 ± 0.058	2.145AspPhe: 2.145 ± 0.046	5.026AspGly: 5.026 ± 0.079	1.209AspHis: 1.209 ± 0.034	2.589AspIle: 2.589 ± 0.062	1.71AspLys: 1.71 ± 0.042	5.249AspLeu: 5.249 ± 0.071	1.086AspMet: 1.086 ± 0.031	1.178AspAsn: 1.178 ± 0.038	3.203AspPro: 3.203 ± 0.061	1.415AspGln: 1.415 ± 0.039	3.589AspArg: 3.589 ± 0.062	2.229AspSer: 2.229 ± 0.039	2.587AspThr: 2.587 ± 0.068	3.645AspVal: 3.645 ± 0.068	0.967AspTrp: 0.967 ± 0.027	1.5AspTyr: 1.5 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	8.33GluAla: 8.33 ± 0.097	0.487GluCys: 0.487 ± 0.02	2.649GluAsp: 2.649 ± 0.052	2.956GluGlu: 2.956 ± 0.059	1.908GluPhe: 1.908 ± 0.043	4.285GluGly: 4.285 ± 0.069	1.719GluHis: 1.719 ± 0.042	3.191GluIle: 3.191 ± 0.056	1.792GluLys: 1.792 ± 0.043	6.929GluLeu: 6.929 ± 0.09	1.315GluMet: 1.315 ± 0.034	1.279GluAsn: 1.279 ± 0.036	2.483GluPro: 2.483 ± 0.047	2.566GluGln: 2.566 ± 0.05	5.927GluArg: 5.927 ± 0.092	2.429GluSer: 2.429 ± 0.05	2.744GluThr: 2.744 ± 0.049	4.466GluVal: 4.466 ± 0.066	0.76GluTrp: 0.76 ± 0.03	1.168GluTyr: 1.168 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	4.652PheAla: 4.652 ± 0.063	0.428PheCys: 0.428 ± 0.021	2.579PheAsp: 2.579 ± 0.053	2.277PheGlu: 2.277 ± 0.047	1.353PhePhe: 1.353 ± 0.037	3.449PheGly: 3.449 ± 0.059	0.806PheHis: 0.806 ± 0.027	1.63PheIle: 1.63 ± 0.042	0.9PheLys: 0.9 ± 0.029	3.124PheLeu: 3.124 ± 0.059	0.718PheMet: 0.718 ± 0.025	0.991PheAsn: 0.991 ± 0.031	1.514PhePro: 1.514 ± 0.037	0.878PheGln: 0.878 ± 0.029	2.275PheArg: 2.275 ± 0.042	1.948PheSer: 1.948 ± 0.042	1.751PheThr: 1.751 ± 0.04	2.768PheVal: 2.768 ± 0.054	0.481PheTrp: 0.481 ± 0.022	0.828PheTyr: 0.828 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	9.89GlyAla: 9.89 ± 0.113	0.992GlyCys: 0.992 ± 0.034	4.077GlyAsp: 4.077 ± 0.071	5.635GlyGlu: 5.635 ± 0.08	3.313GlyPhe: 3.313 ± 0.06	7.301GlyGly: 7.301 ± 0.104	2.007GlyHis: 2.007 ± 0.047	4.07GlyIle: 4.07 ± 0.067	2.987GlyLys: 2.987 ± 0.061	9.372GlyLeu: 9.372 ± 0.115	2.342GlyMet: 2.342 ± 0.052	1.95GlyAsn: 1.95 ± 0.054	2.786GlyPro: 2.786 ± 0.056	2.766GlyGln: 2.766 ± 0.053	6.858GlyArg: 6.858 ± 0.091	4.234GlySer: 4.234 ± 0.069	4.072GlyThr: 4.072 ± 0.058	6.564GlyVal: 6.564 ± 0.087	1.413GlyTrp: 1.413 ± 0.039	2.13GlyTyr: 2.13 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	3.118HisAla: 3.118 ± 0.052	0.297HisCys: 0.297 ± 0.019	1.351HisAsp: 1.351 ± 0.034	1.23HisGlu: 1.23 ± 0.035	1.002HisPhe: 1.002 ± 0.03	2.416HisGly: 2.416 ± 0.05	0.692HisHis: 0.692 ± 0.028	0.99HisIle: 0.99 ± 0.033	0.572HisLys: 0.572 ± 0.022	2.517HisLeu: 2.517 ± 0.046	0.469HisMet: 0.469 ± 0.02	0.48HisAsn: 0.48 ± 0.02	1.679HisPro: 1.679 ± 0.042	0.727HisGln: 0.727 ± 0.026	1.774HisArg: 1.774 ± 0.043	1.047HisSer: 1.047 ± 0.032	1.067HisThr: 1.067 ± 0.032	1.506HisVal: 1.506 ± 0.038	0.407HisTrp: 0.407 ± 0.019	0.676HisTyr: 0.676 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.368IleAla: 6.368 ± 0.082	0.443IleCys: 0.443 ± 0.023	3.466IleAsp: 3.466 ± 0.065	3.602IleGlu: 3.602 ± 0.057	1.281IlePhe: 1.281 ± 0.035	4.381IleGly: 4.381 ± 0.071	1.047IleHis: 1.047 ± 0.028	1.603IleIle: 1.603 ± 0.047	1.293IleLys: 1.293 ± 0.041	3.756IleLeu: 3.756 ± 0.066	0.721IleMet: 0.721 ± 0.028	1.258IleAsn: 1.258 ± 0.035	2.14IlePro: 2.14 ± 0.045	1.142IleGln: 1.142 ± 0.034	3.221IleArg: 3.221 ± 0.059	2.12IleSer: 2.12 ± 0.05	2.325IleThr: 2.325 ± 0.056	3.575IleVal: 3.575 ± 0.059	0.402IleTrp: 0.402 ± 0.021	0.931IleTyr: 0.931 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	3.541LysAla: 3.541 ± 0.074	0.201LysCys: 0.201 ± 0.013	1.436LysAsp: 1.436 ± 0.041	1.384LysGlu: 1.384 ± 0.035	0.822LysPhe: 0.822 ± 0.031	2.156LysGly: 2.156 ± 0.048	0.654LysHis: 0.654 ± 0.023	1.374LysIle: 1.374 ± 0.041	1.168LysLys: 1.168 ± 0.043	3.122LysLeu: 3.122 ± 0.061	0.716LysMet: 0.716 ± 0.027	0.819LysAsn: 0.819 ± 0.029	1.716LysPro: 1.716 ± 0.048	0.993LysGln: 0.993 ± 0.032	2.08LysArg: 2.08 ± 0.05	1.444LysSer: 1.444 ± 0.036	1.545LysThr: 1.545 ± 0.036	2.332LysVal: 2.332 ± 0.052	0.314LysTrp: 0.314 ± 0.017	0.637LysTyr: 0.637 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	16.449LeuAla: 16.449 ± 0.172	1.096LeuCys: 1.096 ± 0.038	6.394LeuAsp: 6.394 ± 0.087	6.581LeuGlu: 6.581 ± 0.096	3.78LeuPhe: 3.78 ± 0.064	9.154LeuGly: 9.154 ± 0.094	2.472LeuHis: 2.472 ± 0.05	4.749LeuIle: 4.749 ± 0.072	3.174LeuLys: 3.174 ± 0.058	11.931LeuLeu: 11.931 ± 0.164	2.32LeuMet: 2.32 ± 0.049	2.446LeuAsn: 2.446 ± 0.05	6.506LeuPro: 6.506 ± 0.087	3.654LeuGln: 3.654 ± 0.067	8.563LeuArg: 8.563 ± 0.104	5.773LeuSer: 5.773 ± 0.079	5.091LeuThr: 5.091 ± 0.077	7.837LeuVal: 7.837 ± 0.099	1.272LeuTrp: 1.272 ± 0.042	2.23LeuTyr: 2.23 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.674MetAla: 2.674 ± 0.055	0.168MetCys: 0.168 ± 0.013	1.003MetAsp: 1.003 ± 0.031	1.05MetGlu: 1.05 ± 0.033	0.708MetPhe: 0.708 ± 0.027	1.588MetGly: 1.588 ± 0.041	0.515MetHis: 0.515 ± 0.02	1.094MetIle: 1.094 ± 0.031	1.037MetLys: 1.037 ± 0.031	2.563MetLeu: 2.563 ± 0.052	0.579MetMet: 0.579 ± 0.026	0.88MetAsn: 0.88 ± 0.029	1.363MetPro: 1.363 ± 0.038	0.825MetGln: 0.825 ± 0.03	1.667MetArg: 1.667 ± 0.038	1.509MetSer: 1.509 ± 0.038	1.276MetThr: 1.276 ± 0.037	1.617MetVal: 1.617 ± 0.046	0.204MetTrp: 0.204 ± 0.013	0.34MetTyr: 0.34 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.886AsnAla: 2.886 ± 0.052	0.257AsnCys: 0.257 ± 0.016	1.116AsnAsp: 1.116 ± 0.033	1.15AsnGlu: 1.15 ± 0.034	0.791AsnPhe: 0.791 ± 0.026	1.893AsnGly: 1.893 ± 0.042	0.539AsnHis: 0.539 ± 0.023	1.106AsnIle: 1.106 ± 0.037	0.658AsnLys: 0.658 ± 0.03	2.556AsnLeu: 2.556 ± 0.05	0.528AsnMet: 0.528 ± 0.025	0.623AsnAsn: 0.623 ± 0.025	1.692AsnPro: 1.692 ± 0.043	0.705AsnGln: 0.705 ± 0.027	1.754AsnArg: 1.754 ± 0.04	0.945AsnSer: 0.945 ± 0.032	1.192AsnThr: 1.192 ± 0.036	1.575AsnVal: 1.575 ± 0.042	0.361AsnTrp: 0.361 ± 0.021	0.583AsnTyr: 0.583 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	7.833ProAla: 7.833 ± 0.116	0.446ProCys: 0.446 ± 0.022	3.046ProAsp: 3.046 ± 0.053	3.559ProGlu: 3.559 ± 0.065	1.796ProPhe: 1.796 ± 0.042	4.805ProGly: 4.805 ± 0.073	1.158ProHis: 1.158 ± 0.032	1.828ProIle: 1.828 ± 0.047	1.251ProLys: 1.251 ± 0.046	5.442ProLeu: 5.442 ± 0.07	1.153ProMet: 1.153 ± 0.033	1.099ProAsn: 1.099 ± 0.035	3.023ProPro: 3.023 ± 0.064	1.851ProGln: 1.851 ± 0.038	3.243ProArg: 3.243 ± 0.058	2.263ProSer: 2.263 ± 0.048	2.195ProThr: 2.195 ± 0.051	4.129ProVal: 4.129 ± 0.065	0.726ProTrp: 0.726 ± 0.028	1.051ProTyr: 1.051 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	5.039GlnAla: 5.039 ± 0.075	0.314GlnCys: 0.314 ± 0.016	1.49GlnAsp: 1.49 ± 0.041	1.497GlnGlu: 1.497 ± 0.037	0.999GlnPhe: 0.999 ± 0.032	2.491GlnGly: 2.491 ± 0.049	0.819GlnHis: 0.819 ± 0.029	1.56GlnIle: 1.56 ± 0.045	0.935GlnLys: 0.935 ± 0.031	3.678GlnLeu: 3.678 ± 0.068	0.797GlnMet: 0.797 ± 0.03	0.653GlnAsn: 0.653 ± 0.023	1.8GlnPro: 1.8 ± 0.043	1.458GlnGln: 1.458 ± 0.041	3.034GlnArg: 3.034 ± 0.056	1.452GlnSer: 1.452 ± 0.038	1.458GlnThr: 1.458 ± 0.043	2.522GlnVal: 2.522 ± 0.052	0.503GlnTrp: 0.503 ± 0.023	0.662GlnTyr: 0.662 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	9.305ArgAla: 9.305 ± 0.128	0.729ArgCys: 0.729 ± 0.029	3.913ArgAsp: 3.913 ± 0.058	4.843ArgGlu: 4.843 ± 0.075	3.182ArgPhe: 3.182 ± 0.058	5.237ArgGly: 5.237 ± 0.07	2.296ArgHis: 2.296 ± 0.053	4.222ArgIle: 4.222 ± 0.058	2.059ArgLys: 2.059 ± 0.047	9.53ArgLeu: 9.53 ± 0.133	1.969ArgMet: 1.969 ± 0.042	1.844ArgAsn: 1.844 ± 0.04	3.792ArgPro: 3.792 ± 0.074	2.797ArgGln: 2.797 ± 0.055	6.57ArgArg: 6.57 ± 0.092	3.818ArgSer: 3.818 ± 0.064	3.406ArgThr: 3.406 ± 0.052	5.388ArgVal: 5.388 ± 0.073	1.175ArgTrp: 1.175 ± 0.033	2.044ArgTyr: 2.044 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.444SerAla: 6.444 ± 0.083	0.5SerCys: 0.5 ± 0.02	2.305SerAsp: 2.305 ± 0.049	2.383SerGlu: 2.383 ± 0.051	1.857SerPhe: 1.857 ± 0.037	4.946SerGly: 4.946 ± 0.075	1.044SerHis: 1.044 ± 0.032	2.234SerIle: 2.234 ± 0.046	1.226SerLys: 1.226 ± 0.037	5.175SerLeu: 5.175 ± 0.069	1.13SerMet: 1.13 ± 0.031	1.063SerAsn: 1.063 ± 0.035	2.571SerPro: 2.571 ± 0.046	1.363SerGln: 1.363 ± 0.035	3.464SerArg: 3.464 ± 0.068	2.436SerSer: 2.436 ± 0.048	2.387SerThr: 2.387 ± 0.043	3.37SerVal: 3.37 ± 0.057	0.663SerTrp: 0.663 ± 0.023	1.055SerTyr: 1.055 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	5.815ThrAla: 5.815 ± 0.085	0.44ThrCys: 0.44 ± 0.022	2.33ThrAsp: 2.33 ± 0.047	2.433ThrGlu: 2.433 ± 0.052	1.504ThrPhe: 1.504 ± 0.034	4.221ThrGly: 4.221 ± 0.071	1.083ThrHis: 1.083 ± 0.031	1.96ThrIle: 1.96 ± 0.049	1.013ThrLys: 1.013 ± 0.031	6.122ThrLeu: 6.122 ± 0.079	0.956ThrMet: 0.956 ± 0.033	0.96ThrAsn: 0.96 ± 0.033	3.17ThrPro: 3.17 ± 0.055	1.4ThrGln: 1.4 ± 0.043	3.518ThrArg: 3.518 ± 0.063	1.987ThrSer: 1.987 ± 0.049	2.361ThrThr: 2.361 ± 0.057	4.062ThrVal: 4.062 ± 0.074	0.563ThrTrp: 0.563 ± 0.024	0.929ThrTyr: 0.929 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	9.547ValAla: 9.547 ± 0.105	0.793ValCys: 0.793 ± 0.026	4.28ValAsp: 4.28 ± 0.065	4.497ValGlu: 4.497 ± 0.069	2.863ValPhe: 2.863 ± 0.052	5.457ValGly: 5.457 ± 0.081	1.736ValHis: 1.736 ± 0.04	3.584ValIle: 3.584 ± 0.06	2.214ValLys: 2.214 ± 0.046	8.587ValLeu: 8.587 ± 0.114	1.756ValMet: 1.756 ± 0.041	1.693ValAsn: 1.693 ± 0.044	3.613ValPro: 3.613 ± 0.061	2.166ValGln: 2.166 ± 0.052	5.672ValArg: 5.672 ± 0.084	3.909ValSer: 3.909 ± 0.063	3.434ValThr: 3.434 ± 0.065	6.136ValVal: 6.136 ± 0.092	0.89ValTrp: 0.89 ± 0.033	1.398ValTyr: 1.398 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.289TrpAla: 1.289 ± 0.033	0.165TrpCys: 0.165 ± 0.012	0.542TrpAsp: 0.542 ± 0.023	0.608TrpGlu: 0.608 ± 0.023	0.532TrpPhe: 0.532 ± 0.021	0.868TrpGly: 0.868 ± 0.024	0.368TrpHis: 0.368 ± 0.021	0.666TrpIle: 0.666 ± 0.025	0.458TrpLys: 0.458 ± 0.022	1.948TrpLeu: 1.948 ± 0.053	0.375TrpMet: 0.375 ± 0.019	0.369TrpAsn: 0.369 ± 0.018	0.674TrpPro: 0.674 ± 0.026	0.72TrpGln: 0.72 ± 0.025	1.209TrpArg: 1.209 ± 0.04	0.697TrpSer: 0.697 ± 0.027	0.617TrpThr: 0.617 ± 0.025	1.014TrpVal: 1.014 ± 0.035	0.244TrpTrp: 0.244 ± 0.015	0.283TrpTyr: 0.283 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.544TyrAla: 2.544 ± 0.051	0.258TyrCys: 0.258 ± 0.015	1.238TyrAsp: 1.238 ± 0.036	1.073TyrGlu: 1.073 ± 0.029	0.859TyrPhe: 0.859 ± 0.03	1.926TyrGly: 1.926 ± 0.045	0.558TyrHis: 0.558 ± 0.024	0.774TyrIle: 0.774 ± 0.025	0.586TyrLys: 0.586 ± 0.024	2.505TyrLeu: 2.505 ± 0.049	0.373TyrMet: 0.373 ± 0.017	0.559TyrAsn: 0.559 ± 0.025	1.141TyrPro: 1.141 ± 0.034	0.756TyrGln: 0.756 ± 0.028	1.962TyrArg: 1.962 ± 0.043	1.02TyrSer: 1.02 ± 0.036	1.06TyrThr: 1.06 ± 0.032	1.55TyrVal: 1.55 ± 0.036	0.324TyrTrp: 0.324 ± 0.016	0.622TyrTyr: 0.622 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3343 proteins (1097188 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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