Cronobacter phage ENT47670
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F1C5E3|F1C5E3_9CAUD Uncharacterized protein OS=Cronobacter phage ENT47670 OX=984186 PE=4 SV=1
MM1 pKa = 8.11 AITPITAAQVKK12 pKa = 9.02 QQLSSLGYY20 pKa = 9.71 SIPDD24 pKa = 4.35 FIIDD28 pKa = 4.38 AYY30 pKa = 10.7 LCKK33 pKa = 10.42 LSSIEE38 pKa = 3.93 QCLEE42 pKa = 3.36 ASGYY46 pKa = 9.39 DD47 pKa = 3.25 EE48 pKa = 5.48 CDD50 pKa = 3.12 VVLIQVYY57 pKa = 9.73 AVSLMALTAYY67 pKa = 8.81 SQRR70 pKa = 11.84 IKK72 pKa = 10.78 SQSAPSGASRR82 pKa = 11.84 SFDD85 pKa = 3.3 YY86 pKa = 11.12 TGDD89 pKa = 3.68 VLSMRR94 pKa = 11.84 DD95 pKa = 3.49 ALLSLDD101 pKa = 4.29 KK102 pKa = 11.08 SGCTASLPIDD112 pKa = 3.48 VGSRR116 pKa = 11.84 VGFFDD121 pKa = 4.66 VVGGCC126 pKa = 4.15
Molecular weight: 13.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.077
IPC2_protein 4.304
IPC_protein 4.215
Toseland 3.999
ProMoST 4.393
Dawson 4.215
Bjellqvist 4.368
Wikipedia 4.177
Rodwell 4.037
Grimsley 3.91
Solomon 4.202
Lehninger 4.164
Nozaki 4.329
DTASelect 4.609
Thurlkill 4.062
EMBOSS 4.177
Sillero 4.342
Patrickios 0.655
IPC_peptide 4.202
IPC2_peptide 4.317
IPC2.peptide.svr19 4.218
Protein with the highest isoelectric point:
>tr|F1C5C1|F1C5C1_9CAUD Uncharacterized protein OS=Cronobacter phage ENT47670 OX=984186 PE=4 SV=1
MM1 pKa = 7.64 RR2 pKa = 11.84 KK3 pKa = 9.97 SNATYY8 pKa = 10.07 AAKK11 pKa = 10.56 ALLRR15 pKa = 11.84 LPFTVAMAMKK25 pKa = 10.16 SCATNAITTFTTLMMKK41 pKa = 10.05 ARR43 pKa = 11.84 KK44 pKa = 8.82 LKK46 pKa = 9.3 PSARR50 pKa = 11.84 RR51 pKa = 11.84 SEE53 pKa = 3.8 WSGLNVASGCRR64 pKa = 11.84 KK65 pKa = 9.52 RR66 pKa = 11.84 MQSSRR71 pKa = 11.84 CSHH74 pKa = 6.9 ASRR77 pKa = 11.84 VATYY81 pKa = 9.69 RR82 pKa = 11.84 LCIILKK88 pKa = 9.97 DD89 pKa = 3.7 DD90 pKa = 4.13 GMTHH94 pKa = 6.74 TEE96 pKa = 3.95 LCQFARR102 pKa = 11.84 MLPTGCHH109 pKa = 5.2 SLPRR113 pKa = 11.84 QLNSSRR119 pKa = 3.65
Molecular weight: 13.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.823
IPC_protein 10.833
Toseland 11.111
ProMoST 11.359
Dawson 11.155
Bjellqvist 10.95
Wikipedia 11.447
Rodwell 11.301
Grimsley 11.184
Solomon 11.418
Lehninger 11.359
Nozaki 11.111
DTASelect 10.95
Thurlkill 11.096
EMBOSS 11.55
Sillero 11.111
Patrickios 11.038
IPC_peptide 11.418
IPC2_peptide 10.35
IPC2.peptide.svr19 8.927
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
11892
102
825
258.5
28.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.612 ± 0.739
1.144 ± 0.175
5.743 ± 0.277
6.029 ± 0.416
3.557 ± 0.289
6.778 ± 0.338
1.623 ± 0.213
5.642 ± 0.26
5.155 ± 0.368
8.022 ± 0.306
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.598 ± 0.202
4.6 ± 0.253
3.809 ± 0.256
4.549 ± 0.404
6.088 ± 0.381
6.971 ± 0.461
6.054 ± 0.415
7.173 ± 0.342
1.589 ± 0.15
3.263 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here