Cronobacter phage ENT47670 
Average proteome isoelectric point is 6.66 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|F1C5E3|F1C5E3_9CAUD Uncharacterized protein OS=Cronobacter phage ENT47670 OX=984186 PE=4 SV=1MM1 pKa = 8.11  AITPITAAQVKK12 pKa = 9.02  QQLSSLGYY20 pKa = 9.71  SIPDD24 pKa = 4.35  FIIDD28 pKa = 4.38  AYY30 pKa = 10.7  LCKK33 pKa = 10.42  LSSIEE38 pKa = 3.93  QCLEE42 pKa = 3.36  ASGYY46 pKa = 9.39  DD47 pKa = 3.25  EE48 pKa = 5.48  CDD50 pKa = 3.12  VVLIQVYY57 pKa = 9.73  AVSLMALTAYY67 pKa = 8.81  SQRR70 pKa = 11.84  IKK72 pKa = 10.78  SQSAPSGASRR82 pKa = 11.84  SFDD85 pKa = 3.3  YY86 pKa = 11.12  TGDD89 pKa = 3.68  VLSMRR94 pKa = 11.84  DD95 pKa = 3.49  ALLSLDD101 pKa = 4.29  KK102 pKa = 11.08  SGCTASLPIDD112 pKa = 3.48  VGSRR116 pKa = 11.84  VGFFDD121 pKa = 4.66  VVGGCC126 pKa = 4.15  
 13.38 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.077 
IPC2_protein 4.304 
IPC_protein 4.215 
Toseland    3.999 
ProMoST     4.393 
Dawson      4.215 
Bjellqvist  4.368 
Wikipedia   4.177 
Rodwell     4.037 
Grimsley    3.91 
Solomon     4.202 
Lehninger   4.164 
Nozaki      4.329 
DTASelect   4.609 
Thurlkill   4.062 
EMBOSS      4.177 
Sillero     4.342 
Patrickios  0.655 
IPC_peptide 4.202 
IPC2_peptide  4.317 
IPC2.peptide.svr19  4.218 
 Protein with the highest isoelectric point: 
>tr|F1C5C1|F1C5C1_9CAUD Uncharacterized protein OS=Cronobacter phage ENT47670 OX=984186 PE=4 SV=1MM1 pKa = 7.64  RR2 pKa = 11.84  KK3 pKa = 9.97  SNATYY8 pKa = 10.07  AAKK11 pKa = 10.56  ALLRR15 pKa = 11.84  LPFTVAMAMKK25 pKa = 10.16  SCATNAITTFTTLMMKK41 pKa = 10.05  ARR43 pKa = 11.84  KK44 pKa = 8.82  LKK46 pKa = 9.3  PSARR50 pKa = 11.84  RR51 pKa = 11.84  SEE53 pKa = 3.8  WSGLNVASGCRR64 pKa = 11.84  KK65 pKa = 9.52  RR66 pKa = 11.84  MQSSRR71 pKa = 11.84  CSHH74 pKa = 6.9  ASRR77 pKa = 11.84  VATYY81 pKa = 9.69  RR82 pKa = 11.84  LCIILKK88 pKa = 9.97  DD89 pKa = 3.7  DD90 pKa = 4.13  GMTHH94 pKa = 6.74  TEE96 pKa = 3.95  LCQFARR102 pKa = 11.84  MLPTGCHH109 pKa = 5.2  SLPRR113 pKa = 11.84  QLNSSRR119 pKa = 3.65  
 13.29 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.297 
IPC2_protein 9.823 
IPC_protein 10.833 
Toseland    11.111 
ProMoST     11.359 
Dawson      11.155 
Bjellqvist  10.95 
Wikipedia   11.447 
Rodwell     11.301 
Grimsley    11.184 
Solomon     11.418 
Lehninger   11.359 
Nozaki      11.111 
DTASelect   10.95 
Thurlkill   11.096 
EMBOSS      11.55 
Sillero     11.111 
Patrickios  11.038 
IPC_peptide 11.418 
IPC2_peptide  10.35 
IPC2.peptide.svr19  8.927 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        46 
0
46 
11892
102
825
258.5
28.74
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        9.612 ± 0.739
1.144 ± 0.175
5.743 ± 0.277
6.029 ± 0.416
3.557 ± 0.289
6.778 ± 0.338
1.623 ± 0.213
5.642 ± 0.26
5.155 ± 0.368
8.022 ± 0.306
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.598 ± 0.202
4.6 ± 0.253
3.809 ± 0.256
4.549 ± 0.404
6.088 ± 0.381
6.971 ± 0.461
6.054 ± 0.415
7.173 ± 0.342
1.589 ± 0.15
3.263 ± 0.269
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here