Conexibacter sp. Seoho-28
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4386 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T4UBY5|A0A2T4UBY5_9ACTN MlaD domain-containing protein OS=Conexibacter sp. Seoho-28 OX=2133960 GN=C7Y72_21930 PE=4 SV=1
MM1 pKa = 8.22 PDD3 pKa = 3.25 PTLADD8 pKa = 3.74 DD9 pKa = 5.66 DD10 pKa = 4.69 FAASLTDD17 pKa = 3.24 SSLYY21 pKa = 10.87 SIGAYY26 pKa = 9.93 FCDD29 pKa = 3.37 RR30 pKa = 11.84 HH31 pKa = 6.19 PEE33 pKa = 3.91 LVEE36 pKa = 4.6 DD37 pKa = 4.19 VLAQSVDD44 pKa = 3.1 IEE46 pKa = 4.44 QQGLRR51 pKa = 11.84 AWATTEE57 pKa = 4.02 GTSVEE62 pKa = 4.47 TAFQTLVTGLAVRR75 pKa = 11.84 YY76 pKa = 8.32 FSAVAGGGG84 pKa = 3.35
Molecular weight: 8.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.516
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.846
Patrickios 1.875
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|A0A2T4UI88|A0A2T4UI88_9ACTN HTH tetR-type domain-containing protein OS=Conexibacter sp. Seoho-28 OX=2133960 GN=C7Y72_04480 PE=4 SV=1
MM1 pKa = 7.19 LRR3 pKa = 11.84 SRR5 pKa = 11.84 RR6 pKa = 11.84 LRR8 pKa = 11.84 SRR10 pKa = 11.84 LRR12 pKa = 11.84 PAPPRR17 pKa = 11.84 PRR19 pKa = 11.84 RR20 pKa = 11.84 SPPRR24 pKa = 11.84 ATAPRR29 pKa = 11.84 PLRR32 pKa = 11.84 PAPRR36 pKa = 11.84 RR37 pKa = 11.84 AARR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 GGRR45 pKa = 11.84 GWRR48 pKa = 11.84 RR49 pKa = 11.84 SPAPSGCPGAWRR61 pKa = 11.84 RR62 pKa = 11.84 SARR65 pKa = 3.51
Molecular weight: 7.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.866
Toseland 13.027
ProMoST 13.525
Dawson 13.027
Bjellqvist 13.027
Wikipedia 13.495
Rodwell 12.515
Grimsley 13.056
Solomon 13.525
Lehninger 13.422
Nozaki 13.027
DTASelect 13.027
Thurlkill 13.027
EMBOSS 13.525
Sillero 13.027
Patrickios 12.237
IPC_peptide 13.525
IPC2_peptide 12.515
IPC2.peptide.svr19 9.228
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4386
0
4386
1458732
29
2462
332.6
35.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.532 ± 0.066
0.8 ± 0.01
6.338 ± 0.032
5.455 ± 0.051
2.712 ± 0.022
9.569 ± 0.046
2.007 ± 0.021
3.319 ± 0.027
1.746 ± 0.029
10.397 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.517 ± 0.015
1.524 ± 0.02
6.132 ± 0.034
2.668 ± 0.02
8.98 ± 0.052
4.453 ± 0.025
6.067 ± 0.04
8.864 ± 0.032
1.23 ± 0.014
1.69 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here