Amino acid dipepetide frequency for Conexibacter sp. Seoho-28

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.823AlaAla: 23.823 ± 0.275
1.162AlaCys: 1.162 ± 0.032
8.766AlaAsp: 8.766 ± 0.083
7.119AlaGlu: 7.119 ± 0.106
4.067AlaPhe: 4.067 ± 0.053
13.767AlaGly: 13.767 ± 0.113
2.55AlaHis: 2.55 ± 0.047
5.058AlaIle: 5.058 ± 0.066
2.416AlaLys: 2.416 ± 0.054
16.03AlaLeu: 16.03 ± 0.155
2.626AlaMet: 2.626 ± 0.048
1.924AlaAsn: 1.924 ± 0.044
8.317AlaPro: 8.317 ± 0.124
4.219AlaGln: 4.219 ± 0.086
12.679AlaArg: 12.679 ± 0.142
6.007AlaSer: 6.007 ± 0.074
8.714AlaThr: 8.714 ± 0.107
12.014AlaVal: 12.014 ± 0.105
1.76AlaTrp: 1.76 ± 0.039
2.303AlaTyr: 2.303 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
1.176CysAla: 1.176 ± 0.034
0.101CysCys: 0.101 ± 0.008
0.527CysAsp: 0.527 ± 0.019
0.464CysGlu: 0.464 ± 0.018
0.25CysPhe: 0.25 ± 0.014
1.039CysGly: 1.039 ± 0.029
0.156CysHis: 0.156 ± 0.01
0.194CysIle: 0.194 ± 0.012
0.121CysLys: 0.121 ± 0.009
0.611CysLeu: 0.611 ± 0.022
0.093CysMet: 0.093 ± 0.008
0.133CysAsn: 0.133 ± 0.009
0.504CysPro: 0.504 ± 0.019
0.16CysGln: 0.16 ± 0.011
0.637CysArg: 0.637 ± 0.023
0.446CysSer: 0.446 ± 0.018
0.475CysThr: 0.475 ± 0.019
0.683CysVal: 0.683 ± 0.023
0.111CysTrp: 0.111 ± 0.011
0.12CysTyr: 0.12 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
9.569AspAla: 9.569 ± 0.081
0.421AspCys: 0.421 ± 0.018
4.367AspAsp: 4.367 ± 0.081
4.194AspGlu: 4.194 ± 0.057
1.331AspPhe: 1.331 ± 0.032
7.664AspGly: 7.664 ± 0.1
1.368AspHis: 1.368 ± 0.035
1.567AspIle: 1.567 ± 0.033
0.826AspLys: 0.826 ± 0.03
6.717AspLeu: 6.717 ± 0.076
0.699AspMet: 0.699 ± 0.02
0.683AspAsn: 0.683 ± 0.024
4.795AspPro: 4.795 ± 0.056
1.383AspGln: 1.383 ± 0.035
5.994AspArg: 5.994 ± 0.076
1.677AspSer: 1.677 ± 0.04
2.733AspThr: 2.733 ± 0.054
5.689AspVal: 5.689 ± 0.071
0.734AspTrp: 0.734 ± 0.024
0.968AspTyr: 0.968 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
6.897GluAla: 6.897 ± 0.111
0.268GluCys: 0.268 ± 0.012
3.003GluAsp: 3.003 ± 0.045
3.187GluGlu: 3.187 ± 0.064
1.179GluPhe: 1.179 ± 0.031
3.916GluGly: 3.916 ± 0.058
1.671GluHis: 1.671 ± 0.038
2.547GluIle: 2.547 ± 0.041
1.09GluLys: 1.09 ± 0.03
7.088GluLeu: 7.088 ± 0.092
0.742GluMet: 0.742 ± 0.024
0.802GluAsn: 0.802 ± 0.026
3.219GluPro: 3.219 ± 0.052
2.194GluGln: 2.194 ± 0.046
6.188GluArg: 6.188 ± 0.09
1.924GluSer: 1.924 ± 0.036
2.813GluThr: 2.813 ± 0.044
4.481GluVal: 4.481 ± 0.059
0.565GluTrp: 0.565 ± 0.021
0.771GluTyr: 0.771 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
3.86PheAla: 3.86 ± 0.053
0.256PheCys: 0.256 ± 0.013
2.011PheAsp: 2.011 ± 0.039
1.483PheGlu: 1.483 ± 0.034
0.906PhePhe: 0.906 ± 0.031
2.998PheGly: 2.998 ± 0.052
0.526PheHis: 0.526 ± 0.023
0.708PheIle: 0.708 ± 0.021
0.483PheLys: 0.483 ± 0.018
2.507PheLeu: 2.507 ± 0.046
0.324PheMet: 0.324 ± 0.017
0.524PheAsn: 0.524 ± 0.017
1.431PhePro: 1.431 ± 0.037
0.662PheGln: 0.662 ± 0.022
1.793PheArg: 1.793 ± 0.033
1.475PheSer: 1.475 ± 0.036
1.826PheThr: 1.826 ± 0.039
2.448PheVal: 2.448 ± 0.041
0.379PheTrp: 0.379 ± 0.016
0.522PheTyr: 0.522 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
12.517GlyAla: 12.517 ± 0.143
0.821GlyCys: 0.821 ± 0.023
5.698GlyAsp: 5.698 ± 0.082
5.122GlyGlu: 5.122 ± 0.064
2.881GlyPhe: 2.881 ± 0.052
9.635GlyGly: 9.635 ± 0.146
1.936GlyHis: 1.936 ± 0.035
3.427GlyIle: 3.427 ± 0.053
1.845GlyLys: 1.845 ± 0.051
9.268GlyLeu: 9.268 ± 0.085
1.772GlyMet: 1.772 ± 0.039
1.592GlyAsn: 1.592 ± 0.047
5.573GlyPro: 5.573 ± 0.078
2.368GlyGln: 2.368 ± 0.04
8.643GlyArg: 8.643 ± 0.1
4.88GlySer: 4.88 ± 0.063
6.602GlyThr: 6.602 ± 0.091
7.901GlyVal: 7.901 ± 0.075
1.329GlyTrp: 1.329 ± 0.032
1.886GlyTyr: 1.886 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.788HisAla: 2.788 ± 0.051
0.191HisCys: 0.191 ± 0.011
1.49HisAsp: 1.49 ± 0.036
1.311HisGlu: 1.311 ± 0.029
0.496HisPhe: 0.496 ± 0.018
2.233HisGly: 2.233 ± 0.04
0.636HisHis: 0.636 ± 0.022
0.422HisIle: 0.422 ± 0.018
0.267HisLys: 0.267 ± 0.013
1.935HisLeu: 1.935 ± 0.042
0.232HisMet: 0.232 ± 0.014
0.272HisAsn: 0.272 ± 0.015
1.42HisPro: 1.42 ± 0.042
0.485HisGln: 0.485 ± 0.019
1.853HisArg: 1.853 ± 0.041
0.612HisSer: 0.612 ± 0.022
0.846HisThr: 0.846 ± 0.024
1.927HisVal: 1.927 ± 0.04
0.254HisTrp: 0.254 ± 0.014
0.4HisTyr: 0.4 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
5.423IleAla: 5.423 ± 0.061
0.274IleCys: 0.274 ± 0.013
2.578IleAsp: 2.578 ± 0.046
2.194IleGlu: 2.194 ± 0.046
0.802IlePhe: 0.802 ± 0.026
3.535IleGly: 3.535 ± 0.056
0.637IleHis: 0.637 ± 0.024
0.853IleIle: 0.853 ± 0.03
0.603IleLys: 0.603 ± 0.023
2.561IleLeu: 2.561 ± 0.05
0.382IleMet: 0.382 ± 0.017
0.596IleAsn: 0.596 ± 0.023
1.811IlePro: 1.811 ± 0.036
0.711IleGln: 0.711 ± 0.025
2.212IleArg: 2.212 ± 0.042
1.527IleSer: 1.527 ± 0.033
2.18IleThr: 2.18 ± 0.045
3.43IleVal: 3.43 ± 0.05
0.328IleTrp: 0.328 ± 0.016
0.55IleTyr: 0.55 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
2.226LysAla: 2.226 ± 0.054
0.091LysCys: 0.091 ± 0.007
1.037LysAsp: 1.037 ± 0.033
0.895LysGlu: 0.895 ± 0.03
0.376LysPhe: 0.376 ± 0.02
1.43LysGly: 1.43 ± 0.043
0.337LysHis: 0.337 ± 0.016
0.773LysIle: 0.773 ± 0.03
0.65LysLys: 0.65 ± 0.028
1.952LysLeu: 1.952 ± 0.049
0.279LysMet: 0.279 ± 0.015
0.339LysAsn: 0.339 ± 0.015
1.111LysPro: 1.111 ± 0.03
0.558LysGln: 0.558 ± 0.024
1.414LysArg: 1.414 ± 0.039
0.757LysSer: 0.757 ± 0.024
1.129LysThr: 1.129 ± 0.036
1.619LysVal: 1.619 ± 0.039
0.162LysTrp: 0.162 ± 0.011
0.322LysTyr: 0.322 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
15.986LeuAla: 15.986 ± 0.142
0.856LeuCys: 0.856 ± 0.028
7.297LeuAsp: 7.297 ± 0.082
5.686LeuGlu: 5.686 ± 0.073
2.349LeuPhe: 2.349 ± 0.046
9.748LeuGly: 9.748 ± 0.091
2.212LeuHis: 2.212 ± 0.037
2.726LeuIle: 2.726 ± 0.05
1.68LeuLys: 1.68 ± 0.05
11.396LeuLeu: 11.396 ± 0.127
1.276LeuMet: 1.276 ± 0.032
1.449LeuAsn: 1.449 ± 0.039
6.089LeuPro: 6.089 ± 0.064
2.763LeuGln: 2.763 ± 0.044
9.858LeuArg: 9.858 ± 0.105
4.402LeuSer: 4.402 ± 0.056
5.818LeuThr: 5.818 ± 0.069
9.839LeuVal: 9.839 ± 0.096
1.041LeuTrp: 1.041 ± 0.032
1.501LeuTyr: 1.501 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
1.856MetAla: 1.856 ± 0.039
0.1MetCys: 0.1 ± 0.009
0.814MetAsp: 0.814 ± 0.024
0.646MetGlu: 0.646 ± 0.023
0.351MetPhe: 0.351 ± 0.015
1.229MetGly: 1.229 ± 0.027
0.273MetHis: 0.273 ± 0.014
0.537MetIle: 0.537 ± 0.023
0.35MetLys: 0.35 ± 0.017
1.532MetLeu: 1.532 ± 0.032
0.248MetMet: 0.248 ± 0.015
0.333MetAsn: 0.333 ± 0.015
1.085MetPro: 1.085 ± 0.03
0.417MetGln: 0.417 ± 0.018
1.412MetArg: 1.412 ± 0.028
1.285MetSer: 1.285 ± 0.031
1.22MetThr: 1.22 ± 0.03
1.134MetVal: 1.134 ± 0.032
0.149MetTrp: 0.149 ± 0.011
0.199MetTyr: 0.199 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.093AsnAla: 2.093 ± 0.037
0.134AsnCys: 0.134 ± 0.009
0.958AsnAsp: 0.958 ± 0.034
0.756AsnGlu: 0.756 ± 0.023
0.415AsnPhe: 0.415 ± 0.017
1.548AsnGly: 1.548 ± 0.045
0.319AsnHis: 0.319 ± 0.016
0.504AsnIle: 0.504 ± 0.02
0.241AsnLys: 0.241 ± 0.014
1.479AsnLeu: 1.479 ± 0.04
0.21AsnMet: 0.21 ± 0.011
0.277AsnAsn: 0.277 ± 0.016
1.258AsnPro: 1.258 ± 0.036
0.432AsnGln: 0.432 ± 0.017
1.217AsnArg: 1.217 ± 0.031
0.611AsnSer: 0.611 ± 0.024
0.835AsnThr: 0.835 ± 0.03
1.44AsnVal: 1.44 ± 0.036
0.174AsnTrp: 0.174 ± 0.01
0.341AsnTyr: 0.341 ± 0.016
0.0AsnXaa: 0.0 ± 0.0
Pro
9.565ProAla: 9.565 ± 0.129
0.408ProCys: 0.408 ± 0.018
4.682ProAsp: 4.682 ± 0.063
3.57ProGlu: 3.57 ± 0.051
1.648ProPhe: 1.648 ± 0.036
6.67ProGly: 6.67 ± 0.082
1.05ProHis: 1.05 ± 0.029
1.69ProIle: 1.69 ± 0.039
1.064ProLys: 1.064 ± 0.036
5.28ProLeu: 5.28 ± 0.068
0.844ProMet: 0.844 ± 0.023
0.926ProAsn: 0.926 ± 0.031
3.578ProPro: 3.578 ± 0.063
1.742ProGln: 1.742 ± 0.03
4.592ProArg: 4.592 ± 0.073
3.033ProSer: 3.033 ± 0.048
3.809ProThr: 3.809 ± 0.064
5.381ProVal: 5.381 ± 0.072
0.815ProTrp: 0.815 ± 0.027
0.977ProTyr: 0.977 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.83GlnAla: 3.83 ± 0.07
0.138GlnCys: 0.138 ± 0.01
1.632GlnAsp: 1.632 ± 0.035
1.612GlnGlu: 1.612 ± 0.051
0.651GlnPhe: 0.651 ± 0.025
2.092GlnGly: 2.092 ± 0.04
0.542GlnHis: 0.542 ± 0.017
1.067GlnIle: 1.067 ± 0.026
0.502GlnLys: 0.502 ± 0.02
3.14GlnLeu: 3.14 ± 0.041
0.396GlnMet: 0.396 ± 0.017
0.458GlnAsn: 0.458 ± 0.016
1.486GlnPro: 1.486 ± 0.033
1.03GlnGln: 1.03 ± 0.035
2.687GlnArg: 2.687 ± 0.047
1.002GlnSer: 1.002 ± 0.028
1.483GlnThr: 1.483 ± 0.035
2.185GlnVal: 2.185 ± 0.04
0.313GlnTrp: 0.313 ± 0.014
0.437GlnTyr: 0.437 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
12.581ArgAla: 12.581 ± 0.142
0.743ArgCys: 0.743 ± 0.025
5.399ArgAsp: 5.399 ± 0.068
5.355ArgGlu: 5.355 ± 0.086
2.717ArgPhe: 2.717 ± 0.042
6.955ArgGly: 6.955 ± 0.086
1.832ArgHis: 1.832 ± 0.04
3.412ArgIle: 3.412 ± 0.041
1.356ArgLys: 1.356 ± 0.035
9.181ArgLeu: 9.181 ± 0.102
1.657ArgMet: 1.657 ± 0.034
1.251ArgAsn: 1.251 ± 0.033
5.505ArgPro: 5.505 ± 0.08
2.093ArgGln: 2.093 ± 0.038
9.883ArgArg: 9.883 ± 0.163
4.201ArgSer: 4.201 ± 0.057
5.521ArgThr: 5.521 ± 0.066
7.215ArgVal: 7.215 ± 0.082
1.262ArgTrp: 1.262 ± 0.028
1.686ArgTyr: 1.686 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
5.935SerAla: 5.935 ± 0.069
0.452SerCys: 0.452 ± 0.02
2.392SerAsp: 2.392 ± 0.038
1.878SerGlu: 1.878 ± 0.037
1.582SerPhe: 1.582 ± 0.035
5.076SerGly: 5.076 ± 0.072
0.714SerHis: 0.714 ± 0.022
1.672SerIle: 1.672 ± 0.032
0.821SerLys: 0.821 ± 0.027
4.006SerLeu: 4.006 ± 0.046
0.89SerMet: 0.89 ± 0.027
0.724SerAsn: 0.724 ± 0.023
2.879SerPro: 2.879 ± 0.048
1.065SerGln: 1.065 ± 0.026
3.634SerArg: 3.634 ± 0.053
2.593SerSer: 2.593 ± 0.057
3.105SerThr: 3.105 ± 0.051
3.519SerVal: 3.519 ± 0.061
0.693SerTrp: 0.693 ± 0.02
0.898SerTyr: 0.898 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
8.416ThrAla: 8.416 ± 0.098
0.506ThrCys: 0.506 ± 0.022
3.264ThrAsp: 3.264 ± 0.055
2.434ThrGlu: 2.434 ± 0.047
1.935ThrPhe: 1.935 ± 0.038
6.109ThrGly: 6.109 ± 0.091
0.952ThrHis: 0.952 ± 0.026
2.434ThrIle: 2.434 ± 0.055
1.095ThrLys: 1.095 ± 0.032
6.215ThrLeu: 6.215 ± 0.069
0.885ThrMet: 0.885 ± 0.026
1.027ThrAsn: 1.027 ± 0.028
4.396ThrPro: 4.396 ± 0.074
1.313ThrGln: 1.313 ± 0.031
4.285ThrArg: 4.285 ± 0.059
2.791ThrSer: 2.791 ± 0.049
4.261ThrThr: 4.261 ± 0.08
6.41ThrVal: 6.41 ± 0.085
0.785ThrTrp: 0.785 ± 0.025
1.151ThrTyr: 1.151 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
13.074ValAla: 13.074 ± 0.126
0.768ValCys: 0.768 ± 0.024
5.564ValAsp: 5.564 ± 0.066
4.948ValGlu: 4.948 ± 0.059
2.229ValPhe: 2.229 ± 0.04
7.435ValGly: 7.435 ± 0.073
1.795ValHis: 1.795 ± 0.041
2.667ValIle: 2.667 ± 0.049
1.468ValLys: 1.468 ± 0.041
10.016ValLeu: 10.016 ± 0.109
1.159ValMet: 1.159 ± 0.032
1.365ValAsn: 1.365 ± 0.035
5.431ValPro: 5.431 ± 0.069
2.314ValGln: 2.314 ± 0.042
7.96ValArg: 7.96 ± 0.082
3.873ValSer: 3.873 ± 0.061
5.521ValThr: 5.521 ± 0.085
8.834ValVal: 8.834 ± 0.096
0.937ValTrp: 0.937 ± 0.025
1.284ValTyr: 1.284 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.346TrpAla: 1.346 ± 0.032
0.134TrpCys: 0.134 ± 0.01
0.676TrpAsp: 0.676 ± 0.026
0.631TrpGlu: 0.631 ± 0.023
0.437TrpPhe: 0.437 ± 0.017
0.901TrpGly: 0.901 ± 0.028
0.263TrpHis: 0.263 ± 0.014
0.546TrpIle: 0.546 ± 0.017
0.264TrpLys: 0.264 ± 0.014
1.371TrpLeu: 1.371 ± 0.037
0.28TrpMet: 0.28 ± 0.015
0.287TrpAsn: 0.287 ± 0.015
0.582TrpPro: 0.582 ± 0.022
0.385TrpGln: 0.385 ± 0.013
1.177TrpArg: 1.177 ± 0.027
0.732TrpSer: 0.732 ± 0.022
0.859TrpThr: 0.859 ± 0.025
0.906TrpVal: 0.906 ± 0.029
0.274TrpTrp: 0.274 ± 0.016
0.244TrpTyr: 0.244 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.358TyrAla: 2.358 ± 0.043
0.177TyrCys: 0.177 ± 0.01
1.224TyrAsp: 1.224 ± 0.03
1.06TyrGlu: 1.06 ± 0.03
0.521TyrPhe: 0.521 ± 0.019
1.713TyrGly: 1.713 ± 0.036
0.302TyrHis: 0.302 ± 0.016
0.383TyrIle: 0.383 ± 0.017
0.313TyrLys: 0.313 ± 0.015
1.686TyrLeu: 1.686 ± 0.035
0.175TyrMet: 0.175 ± 0.012
0.283TyrAsn: 0.283 ± 0.013
0.853TyrPro: 0.853 ± 0.027
0.388TyrGln: 0.388 ± 0.014
1.688TyrArg: 1.688 ± 0.038
0.698TyrSer: 0.698 ± 0.022
0.915TyrThr: 0.915 ± 0.027
1.592TyrVal: 1.592 ± 0.038
0.23TyrTrp: 0.23 ± 0.013
0.339TyrTyr: 0.339 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4386 proteins (1458732 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski