Jiangella asiatica
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6206 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R5DLG4|A0A4R5DLG4_9ACTN VOC family protein OS=Jiangella asiatica OX=2530372 GN=E1269_09470 PE=4 SV=1
MM1 pKa = 7.63 AARR4 pKa = 11.84 AGAAALGIAALVSACGGGGEE24 pKa = 4.6 EE25 pKa = 5.15 VDD27 pKa = 3.71 VDD29 pKa = 4.5 DD30 pKa = 6.27 GNGTSGADD38 pKa = 3.22 QGGGDD43 pKa = 3.95 GDD45 pKa = 4.17 APAGTLVAAISAQPDD60 pKa = 3.11 QFDD63 pKa = 3.48 PHH65 pKa = 6.08 KK66 pKa = 8.27 TQAYY70 pKa = 10.15 ASFQVLEE77 pKa = 4.21 NVYY80 pKa = 9.04 DD81 pKa = 3.9 TLVVPDD87 pKa = 4.79 AEE89 pKa = 5.69 GEE91 pKa = 4.27 FQPSLATEE99 pKa = 4.23 WTTSDD104 pKa = 5.56 DD105 pKa = 3.52 ALTWTFTLRR114 pKa = 11.84 EE115 pKa = 4.25 GVTFHH120 pKa = 7.47 DD121 pKa = 5.21 GSAFDD126 pKa = 3.98 SADD129 pKa = 2.91 VVYY132 pKa = 10.33 SYY134 pKa = 11.3 RR135 pKa = 11.84 RR136 pKa = 11.84 IIDD139 pKa = 3.47 EE140 pKa = 4.05 QLANAYY146 pKa = 9.95 RR147 pKa = 11.84 FASVANIDD155 pKa = 3.9 APDD158 pKa = 4.13 PQTVVITLSTPTPALLDD175 pKa = 4.02 NIGGFKK181 pKa = 10.51 GMAIIPEE188 pKa = 4.43 GAGQQTDD195 pKa = 3.56 LATTAVGTGPFTLEE209 pKa = 3.52 EE210 pKa = 4.11 TGAGGVTLARR220 pKa = 11.84 YY221 pKa = 9.7 DD222 pKa = 4.83 DD223 pKa = 4.09 YY224 pKa = 11.26 WGDD227 pKa = 3.44 PATVEE232 pKa = 4.25 GVEE235 pKa = 4.06 FRR237 pKa = 11.84 YY238 pKa = 10.49 VSEE241 pKa = 3.99 PAAALTALRR250 pKa = 11.84 SGDD253 pKa = 3.31 IHH255 pKa = 5.96 WTDD258 pKa = 3.37 NVPPQDD264 pKa = 4.07 IEE266 pKa = 4.29 GLQDD270 pKa = 3.67 DD271 pKa = 4.94 EE272 pKa = 4.44 EE273 pKa = 5.3 VEE275 pKa = 4.57 LGVTPSVDD283 pKa = 3.06 YY284 pKa = 9.78 WYY286 pKa = 11.06 LAMNQTRR293 pKa = 11.84 PPFDD297 pKa = 3.14 NRR299 pKa = 11.84 DD300 pKa = 3.3 FRR302 pKa = 11.84 RR303 pKa = 11.84 GVAYY307 pKa = 10.06 GIDD310 pKa = 3.39 RR311 pKa = 11.84 EE312 pKa = 4.53 EE313 pKa = 3.89 VTEE316 pKa = 3.92 AARR319 pKa = 11.84 FGAATTNQTAIPEE332 pKa = 4.05 QSLWYY337 pKa = 10.06 HH338 pKa = 6.62 EE339 pKa = 4.32 YY340 pKa = 11.22 APFEE344 pKa = 4.53 HH345 pKa = 7.51 DD346 pKa = 3.58 PDD348 pKa = 3.56 QAADD352 pKa = 4.56 LIEE355 pKa = 4.16 QTAAATPQPMGLMVTDD371 pKa = 5.18 EE372 pKa = 4.32 YY373 pKa = 10.95 PEE375 pKa = 4.32 TVQAAQVIQAQLAEE389 pKa = 4.25 VGVEE393 pKa = 3.59 IGIEE397 pKa = 3.93 QEE399 pKa = 5.82 AFATWLDD406 pKa = 3.56 RR407 pKa = 11.84 QAAGDD412 pKa = 3.79 YY413 pKa = 11.03 DD414 pKa = 4.33 AFLLGWLGNLDD425 pKa = 3.53 PFGFYY430 pKa = 10.0 HH431 pKa = 6.36 AQHH434 pKa = 5.75 VCEE437 pKa = 4.48 GTSNFQGYY445 pKa = 9.78 CNPEE449 pKa = 3.53 VDD451 pKa = 3.67 TLLNQAAAEE460 pKa = 4.12 TDD462 pKa = 3.39 QEE464 pKa = 4.16 ARR466 pKa = 11.84 KK467 pKa = 9.78 ALYY470 pKa = 10.04 DD471 pKa = 3.48 QAAEE475 pKa = 4.73 LIVDD479 pKa = 3.99 DD480 pKa = 4.67 VSYY483 pKa = 10.96 LYY485 pKa = 10.58 LYY487 pKa = 10.65 NPDD490 pKa = 4.08 VVQAWAPGLEE500 pKa = 4.5 GYY502 pKa = 9.03 EE503 pKa = 3.9 IRR505 pKa = 11.84 PDD507 pKa = 3.15 KK508 pKa = 10.89 AINFEE513 pKa = 4.46 TVRR516 pKa = 11.84 LPEE519 pKa = 3.86
Molecular weight: 55.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.77
IPC_protein 3.795
Toseland 3.579
ProMoST 3.923
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.91
Patrickios 1.163
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A4R5CFL7|A0A4R5CFL7_9ACTN Uncharacterized protein OS=Jiangella asiatica OX=2530372 GN=E1269_28210 PE=4 SV=1
PP1 pKa = 7.17 HH2 pKa = 7.43 RR3 pKa = 11.84 NLRR6 pKa = 11.84 PGPRR10 pKa = 11.84 PRR12 pKa = 11.84 PRR14 pKa = 11.84 HH15 pKa = 5.02 PHH17 pKa = 4.75 HH18 pKa = 6.21 QRR20 pKa = 11.84 PHH22 pKa = 6.16 RR23 pKa = 11.84 RR24 pKa = 11.84 TPTRR28 pKa = 11.84 ARR30 pKa = 11.84 PGPHH34 pKa = 5.2 THH36 pKa = 6.95 LPAHH40 pKa = 7.07 RR41 pKa = 11.84 PPPGPTRR48 pKa = 11.84 KK49 pKa = 9.79
Molecular weight: 5.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.574
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.31
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.231
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6206
0
6206
2091672
33
3131
337.0
36.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.357 ± 0.041
0.682 ± 0.008
6.754 ± 0.03
5.571 ± 0.025
2.826 ± 0.019
9.375 ± 0.028
2.179 ± 0.014
3.459 ± 0.02
1.317 ± 0.015
10.035 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.757 ± 0.011
1.774 ± 0.015
5.928 ± 0.024
2.614 ± 0.013
7.955 ± 0.041
5.132 ± 0.018
6.115 ± 0.028
9.433 ± 0.031
1.616 ± 0.014
2.121 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here