Polyangium sp. SDU3-1
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9297 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4U1HM64|A0A4U1HM64_9DELT ATP-grasp domain-containing protein OS=Polyangium sp. SDU3-1 OX=2567896 GN=E8A73_12010 PE=4 SV=1
MM1 pKa = 7.06 NTTTLARR8 pKa = 11.84 SFVSVAALAATLFSFAACSATVVQGGDD35 pKa = 3.45 DD36 pKa = 3.74 TDD38 pKa = 3.94 TNSAQIKK45 pKa = 9.56 GGNGPKK51 pKa = 10.11 KK52 pKa = 8.27 STAMAMLYY60 pKa = 10.37 SEE62 pKa = 5.09 LPEE65 pKa = 4.41 VSDD68 pKa = 4.12 GDD70 pKa = 3.83 SGGTSGSSGGGEE82 pKa = 3.91 PGIDD86 pKa = 3.47 PNTLYY91 pKa = 10.15 IYY93 pKa = 10.59 LSSQGMVCSDD103 pKa = 3.0 PHH105 pKa = 5.95 ATLEE109 pKa = 5.11 CGNQWRR115 pKa = 11.84 VSIGIPPEE123 pKa = 3.9 LQKK126 pKa = 11.32 VGIIPLSTPGLIAFATSTGADD147 pKa = 3.83 HH148 pKa = 7.87 GDD150 pKa = 4.08 GTCSWGGGSFLDD162 pKa = 3.86 GTLEE166 pKa = 3.59 ITAIDD171 pKa = 3.93 DD172 pKa = 3.56 THH174 pKa = 6.95 VAGVLADD181 pKa = 3.37 TWGVDD186 pKa = 3.77 FDD188 pKa = 5.32 ADD190 pKa = 3.96 GAFDD194 pKa = 4.52 AALCQFF200 pKa = 4.42
Molecular weight: 20.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.808
IPC_protein 3.808
Toseland 3.579
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.63
Grimsley 3.49
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.215
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.935
Patrickios 0.998
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A4U1HV07|A0A4U1HV07_9DELT YicC family protein OS=Polyangium sp. SDU3-1 OX=2567896 GN=E8A73_07825 PE=4 SV=1
MM1 pKa = 7.07 QPSPPSRR8 pKa = 11.84 ARR10 pKa = 11.84 PWLAGRR16 pKa = 11.84 PPSPSRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 TAARR28 pKa = 11.84 IAPRR32 pKa = 11.84 SRR34 pKa = 11.84 ASIRR38 pKa = 11.84 PRR40 pKa = 11.84 SSSRR44 pKa = 11.84 SPRR47 pKa = 11.84 ARR49 pKa = 11.84 TPTRR53 pKa = 11.84 TTTGRR58 pKa = 11.84 ASTSGRR64 pKa = 11.84 RR65 pKa = 11.84 GICGSTRR72 pKa = 11.84 SSSSS76 pKa = 2.84
Molecular weight: 8.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.506
IPC2_protein 11.155
IPC_protein 12.764
Toseland 12.925
ProMoST 13.422
Dawson 12.925
Bjellqvist 12.925
Wikipedia 13.408
Rodwell 12.427
Grimsley 12.969
Solomon 13.422
Lehninger 13.334
Nozaki 12.925
DTASelect 12.925
Thurlkill 12.925
EMBOSS 13.422
Sillero 12.925
Patrickios 12.149
IPC_peptide 13.437
IPC2_peptide 12.427
IPC2.peptide.svr19 9.192
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9297
0
9297
3626891
30
3772
390.1
42.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.077 ± 0.041
1.191 ± 0.018
5.713 ± 0.018
6.478 ± 0.022
3.162 ± 0.014
9.179 ± 0.03
2.028 ± 0.011
3.876 ± 0.015
3.054 ± 0.021
9.86 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.95 ± 0.01
2.037 ± 0.017
6.285 ± 0.025
2.656 ± 0.014
8.064 ± 0.034
5.498 ± 0.018
4.987 ± 0.019
7.639 ± 0.019
1.274 ± 0.011
1.992 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here