Escherichia alba

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L6IRN4|A0A6L6IRN4_9ESCH DNA-binding protein OS=Escherichia alba OX=2562891 GN=GJV78_22245 PE=4 SV=1
MM1 pKa = 7.45SLLKK5 pKa = 10.1TEE7 pKa = 4.45LVLPFEE13 pKa = 5.15DD14 pKa = 4.92EE15 pKa = 4.17YY16 pKa = 11.73SAMSFWDD23 pKa = 3.74EE24 pKa = 4.19VGVTLIYY31 pKa = 10.57VEE33 pKa = 4.68PDD35 pKa = 3.52DD36 pKa = 4.11VPEE39 pKa = 5.01DD40 pKa = 3.25IKK42 pKa = 11.54SQVLNVIHH50 pKa = 5.98QLIVNPEE57 pKa = 3.89FVVRR61 pKa = 11.84LTDD64 pKa = 4.12DD65 pKa = 3.87YY66 pKa = 11.81YY67 pKa = 12.15LMLSVTEE74 pKa = 4.53DD75 pKa = 3.41NGNGIYY81 pKa = 10.76LLFHH85 pKa = 7.31PDD87 pKa = 3.7CPLKK91 pKa = 11.1GIDD94 pKa = 4.63LLISMAEE101 pKa = 3.7SRR103 pKa = 11.84YY104 pKa = 10.42

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L6IR25|A0A6L6IR25_9ESCH Uncharacterized protein OS=Escherichia alba OX=2562891 GN=GJV78_21115 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4335

0

4335

1333019

14

2860

307.5

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.207 ± 0.048

1.128 ± 0.015

5.119 ± 0.026

5.716 ± 0.041

3.753 ± 0.022

7.637 ± 0.044

2.136 ± 0.017

5.649 ± 0.034

4.041 ± 0.036

10.92 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.02

3.577 ± 0.028

4.556 ± 0.028

4.235 ± 0.032

6.359 ± 0.034

5.873 ± 0.032

5.227 ± 0.031

6.936 ± 0.034

1.545 ± 0.018

2.775 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski