Amino acid dipepetide frequency for Escherichia alba

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.35AlaAla: 11.35 ± 0.119	1.248AlaCys: 1.248 ± 0.034	5.326AlaAsp: 5.326 ± 0.07	5.933AlaGlu: 5.933 ± 0.087	3.596AlaPhe: 3.596 ± 0.052	8.337AlaGly: 8.337 ± 0.097	1.857AlaHis: 1.857 ± 0.038	5.921AlaIle: 5.921 ± 0.073	3.56AlaLys: 3.56 ± 0.063	12.84AlaLeu: 12.84 ± 0.139	2.869AlaMet: 2.869 ± 0.046	2.92AlaAsn: 2.92 ± 0.056	3.969AlaPro: 3.969 ± 0.071	4.427AlaGln: 4.427 ± 0.065	6.756AlaArg: 6.756 ± 0.087	5.632AlaSer: 5.632 ± 0.081	4.782AlaThr: 4.782 ± 0.066	7.018AlaVal: 7.018 ± 0.076	1.846AlaTrp: 1.846 ± 0.043	1.881AlaTyr: 1.881 ± 0.039	0.001AlaXaa: 0.001 ± 0.001
Cys	1.094CysAla: 1.094 ± 0.029	0.177CysCys: 0.177 ± 0.012	0.616CysAsp: 0.616 ± 0.021	0.629CysGlu: 0.629 ± 0.023	0.461CysPhe: 0.461 ± 0.019	1.155CysGly: 1.155 ± 0.03	0.317CysHis: 0.317 ± 0.018	0.608CysIle: 0.608 ± 0.021	0.31CysLys: 0.31 ± 0.018	1.083CysLeu: 1.083 ± 0.027	0.231CysMet: 0.231 ± 0.015	0.297CysAsn: 0.297 ± 0.015	0.561CysPro: 0.561 ± 0.025	0.447CysGln: 0.447 ± 0.016	0.802CysArg: 0.802 ± 0.03	0.671CysSer: 0.671 ± 0.023	0.496CysThr: 0.496 ± 0.021	0.789CysVal: 0.789 ± 0.027	0.204CysTrp: 0.204 ± 0.014	0.332CysTyr: 0.332 ± 0.017	0.001CysXaa: 0.001 ± 0.001
Asp	5.407AspAla: 5.407 ± 0.075	0.507AspCys: 0.507 ± 0.018	2.953AspAsp: 2.953 ± 0.054	3.592AspGlu: 3.592 ± 0.061	2.083AspPhe: 2.083 ± 0.039	4.008AspGly: 4.008 ± 0.069	0.815AspHis: 0.815 ± 0.026	3.395AspIle: 3.395 ± 0.055	2.371AspLys: 2.371 ± 0.044	4.575AspLeu: 4.575 ± 0.069	1.269AspMet: 1.269 ± 0.032	2.061AspAsn: 2.061 ± 0.041	2.268AspPro: 2.268 ± 0.041	1.2AspGln: 1.2 ± 0.036	3.302AspArg: 3.302 ± 0.056	2.725AspSer: 2.725 ± 0.053	2.455AspThr: 2.455 ± 0.051	3.547AspVal: 3.547 ± 0.065	0.787AspTrp: 0.787 ± 0.029	1.87AspTyr: 1.87 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	6.13GluAla: 6.13 ± 0.091	0.513GluCys: 0.513 ± 0.021	2.223GluAsp: 2.223 ± 0.049	3.58GluGlu: 3.58 ± 0.068	1.731GluPhe: 1.731 ± 0.034	3.755GluGly: 3.755 ± 0.055	1.335GluHis: 1.335 ± 0.026	3.347GluIle: 3.347 ± 0.061	3.341GluLys: 3.341 ± 0.055	5.734GluLeu: 5.734 ± 0.076	1.815GluMet: 1.815 ± 0.038	2.257GluAsn: 2.257 ± 0.044	2.125GluPro: 2.125 ± 0.045	3.159GluGln: 3.159 ± 0.055	3.98GluArg: 3.98 ± 0.061	3.076GluSer: 3.076 ± 0.045	2.92GluThr: 2.92 ± 0.047	3.828GluVal: 3.828 ± 0.065	0.8GluTrp: 0.8 ± 0.027	1.51GluTyr: 1.51 ± 0.034	0.002GluXaa: 0.002 ± 0.001
Phe	3.665PheAla: 3.665 ± 0.065	0.554PheCys: 0.554 ± 0.019	2.228PheAsp: 2.228 ± 0.039	1.668PheGlu: 1.668 ± 0.035	1.659PhePhe: 1.659 ± 0.045	3.061PheGly: 3.061 ± 0.059	0.786PheHis: 0.786 ± 0.026	2.569PheIle: 2.569 ± 0.05	1.185PheLys: 1.185 ± 0.03	3.226PheLeu: 3.226 ± 0.063	0.914PheMet: 0.914 ± 0.033	1.662PheAsn: 1.662 ± 0.032	1.512PhePro: 1.512 ± 0.036	1.017PheGln: 1.017 ± 0.029	2.017PheArg: 2.017 ± 0.045	3.2PheSer: 3.2 ± 0.055	2.452PheThr: 2.452 ± 0.045	2.332PheVal: 2.332 ± 0.054	0.617PheTrp: 0.617 ± 0.022	1.209PheTyr: 1.209 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	6.681GlyAla: 6.681 ± 0.096	1.074GlyCys: 1.074 ± 0.031	3.851GlyAsp: 3.851 ± 0.068	4.992GlyGlu: 4.992 ± 0.067	3.211GlyPhe: 3.211 ± 0.063	5.75GlyGly: 5.75 ± 0.089	1.615GlyHis: 1.615 ± 0.037	4.892GlyIle: 4.892 ± 0.066	3.779GlyLys: 3.779 ± 0.06	7.659GlyLeu: 7.659 ± 0.094	2.32GlyMet: 2.32 ± 0.046	2.655GlyAsn: 2.655 ± 0.056	2.091GlyPro: 2.091 ± 0.039	3.084GlyGln: 3.084 ± 0.048	4.508GlyArg: 4.508 ± 0.059	4.464GlySer: 4.464 ± 0.086	3.755GlyThr: 3.755 ± 0.067	5.933GlyVal: 5.933 ± 0.066	1.398GlyTrp: 1.398 ± 0.035	2.654GlyTyr: 2.654 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	1.835HisAla: 1.835 ± 0.036	0.296HisCys: 0.296 ± 0.015	1.107HisAsp: 1.107 ± 0.03	1.037HisGlu: 1.037 ± 0.028	0.97HisPhe: 0.97 ± 0.029	1.738HisGly: 1.738 ± 0.042	0.65HisHis: 0.65 ± 0.024	1.286HisIle: 1.286 ± 0.036	0.723HisLys: 0.723 ± 0.024	2.161HisLeu: 2.161 ± 0.042	0.475HisMet: 0.475 ± 0.02	0.764HisAsn: 0.764 ± 0.024	1.302HisPro: 1.302 ± 0.039	0.986HisGln: 0.986 ± 0.033	1.423HisArg: 1.423 ± 0.032	1.195HisSer: 1.195 ± 0.035	1.022HisThr: 1.022 ± 0.03	1.205HisVal: 1.205 ± 0.03	0.327HisTrp: 0.327 ± 0.015	0.853HisTyr: 0.853 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.45IleAla: 6.45 ± 0.077	0.684IleCys: 0.684 ± 0.024	3.492IleAsp: 3.492 ± 0.052	3.12IleGlu: 3.12 ± 0.05	2.072IlePhe: 2.072 ± 0.046	4.582IleGly: 4.582 ± 0.069	1.149IleHis: 1.149 ± 0.032	3.209IleIle: 3.209 ± 0.061	2.298IleLys: 2.298 ± 0.046	4.834IleLeu: 4.834 ± 0.069	1.191IleMet: 1.191 ± 0.033	2.614IleAsn: 2.614 ± 0.053	2.731IlePro: 2.731 ± 0.045	1.708IleGln: 1.708 ± 0.037	3.121IleArg: 3.121 ± 0.042	3.642IleSer: 3.642 ± 0.055	3.256IleThr: 3.256 ± 0.059	4.094IleVal: 4.094 ± 0.054	0.695IleTrp: 0.695 ± 0.025	1.55IleTyr: 1.55 ± 0.038	0.001IleXaa: 0.001 ± 0.001
Lys	4.141LysAla: 4.141 ± 0.065	0.32LysCys: 0.32 ± 0.014	1.869LysAsp: 1.869 ± 0.047	2.471LysGlu: 2.471 ± 0.049	1.07LysPhe: 1.07 ± 0.029	2.87LysGly: 2.87 ± 0.046	0.77LysHis: 0.77 ± 0.025	2.421LysIle: 2.421 ± 0.053	2.35LysLys: 2.35 ± 0.049	3.868LysLeu: 3.868 ± 0.056	1.214LysMet: 1.214 ± 0.031	1.767LysAsn: 1.767 ± 0.039	1.912LysPro: 1.912 ± 0.038	1.692LysGln: 1.692 ± 0.038	2.528LysArg: 2.528 ± 0.042	2.32LysSer: 2.32 ± 0.041	2.459LysThr: 2.459 ± 0.044	2.74LysVal: 2.74 ± 0.052	0.443LysTrp: 0.443 ± 0.019	1.191LysTyr: 1.191 ± 0.034	0.001LysXaa: 0.001 ± 0.001
Leu	11.991LeuAla: 11.991 ± 0.126	1.377LeuCys: 1.377 ± 0.031	5.23LeuAsp: 5.23 ± 0.074	5.527LeuGlu: 5.527 ± 0.07	4.284LeuPhe: 4.284 ± 0.072	7.198LeuGly: 7.198 ± 0.094	2.229LeuHis: 2.229 ± 0.045	5.845LeuIle: 5.845 ± 0.088	4.414LeuLys: 4.414 ± 0.072	12.457LeuLeu: 12.457 ± 0.161	2.812LeuMet: 2.812 ± 0.051	4.287LeuAsn: 4.287 ± 0.062	5.774LeuPro: 5.774 ± 0.078	4.112LeuGln: 4.112 ± 0.065	6.769LeuArg: 6.769 ± 0.071	7.485LeuSer: 7.485 ± 0.087	6.495LeuThr: 6.495 ± 0.085	6.674LeuVal: 6.674 ± 0.083	1.557LeuTrp: 1.557 ± 0.04	2.681LeuTyr: 2.681 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.925MetAla: 2.925 ± 0.045	0.186MetCys: 0.186 ± 0.011	1.15MetAsp: 1.15 ± 0.027	1.22MetGlu: 1.22 ± 0.029	0.805MetPhe: 0.805 ± 0.026	1.777MetGly: 1.777 ± 0.034	0.51MetHis: 0.51 ± 0.019	1.284MetIle: 1.284 ± 0.036	1.364MetLys: 1.364 ± 0.03	2.983MetLeu: 2.983 ± 0.053	0.812MetMet: 0.812 ± 0.025	1.07MetAsn: 1.07 ± 0.031	1.321MetPro: 1.321 ± 0.031	1.127MetGln: 1.127 ± 0.03	1.56MetArg: 1.56 ± 0.035	1.761MetSer: 1.761 ± 0.04	1.674MetThr: 1.674 ± 0.034	1.805MetVal: 1.805 ± 0.038	0.245MetTrp: 0.245 ± 0.013	0.513MetTyr: 0.513 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.508AsnAla: 3.508 ± 0.05	0.334AsnCys: 0.334 ± 0.017	1.992AsnAsp: 1.992 ± 0.047	1.85AsnGlu: 1.85 ± 0.036	1.263AsnPhe: 1.263 ± 0.032	3.144AsnGly: 3.144 ± 0.065	0.784AsnHis: 0.784 ± 0.023	2.281AsnIle: 2.281 ± 0.045	1.493AsnLys: 1.493 ± 0.039	3.371AsnLeu: 3.371 ± 0.05	0.851AsnMet: 0.851 ± 0.029	1.557AsnAsn: 1.557 ± 0.039	1.995AsnPro: 1.995 ± 0.041	1.389AsnGln: 1.389 ± 0.031	2.131AsnArg: 2.131 ± 0.04	1.903AsnSer: 1.903 ± 0.047	1.83AsnThr: 1.83 ± 0.041	2.514AsnVal: 2.514 ± 0.054	0.46AsnTrp: 0.46 ± 0.019	1.126AsnTyr: 1.126 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	4.93ProAla: 4.93 ± 0.07	0.427ProCys: 0.427 ± 0.02	2.876ProAsp: 2.876 ± 0.051	3.567ProGlu: 3.567 ± 0.054	1.814ProPhe: 1.814 ± 0.037	3.787ProGly: 3.787 ± 0.055	0.989ProHis: 0.989 ± 0.028	1.641ProIle: 1.641 ± 0.038	1.376ProLys: 1.376 ± 0.034	5.276ProLeu: 5.276 ± 0.084	1.024ProMet: 1.024 ± 0.026	1.158ProAsn: 1.158 ± 0.027	1.911ProPro: 1.911 ± 0.049	2.354ProGln: 2.354 ± 0.055	2.289ProArg: 2.289 ± 0.04	2.054ProSer: 2.054 ± 0.043	2.064ProThr: 2.064 ± 0.047	4.051ProVal: 4.051 ± 0.053	0.829ProTrp: 0.829 ± 0.026	1.141ProTyr: 1.141 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.635GlnAla: 4.635 ± 0.065	0.368GlnCys: 0.368 ± 0.017	1.635GlnAsp: 1.635 ± 0.036	2.221GlnGlu: 2.221 ± 0.048	1.294GlnPhe: 1.294 ± 0.031	2.969GlnGly: 2.969 ± 0.049	1.045GlnHis: 1.045 ± 0.027	2.228GlnIle: 2.228 ± 0.04	1.778GlnLys: 1.778 ± 0.037	4.737GlnLeu: 4.737 ± 0.073	1.122GlnMet: 1.122 ± 0.03	1.406GlnAsn: 1.406 ± 0.04	2.16GlnPro: 2.16 ± 0.052	2.948GlnGln: 2.948 ± 0.067	3.136GlnArg: 3.136 ± 0.056	2.143GlnSer: 2.143 ± 0.046	2.122GlnThr: 2.122 ± 0.042	2.66GlnVal: 2.66 ± 0.049	0.595GlnTrp: 0.595 ± 0.021	1.147GlnTyr: 1.147 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	5.511ArgAla: 5.511 ± 0.073	0.734ArgCys: 0.734 ± 0.023	3.189ArgAsp: 3.189 ± 0.053	4.223ArgGlu: 4.223 ± 0.073	2.782ArgPhe: 2.782 ± 0.05	3.788ArgGly: 3.788 ± 0.06	1.686ArgHis: 1.686 ± 0.049	3.474ArgIle: 3.474 ± 0.049	2.299ArgLys: 2.299 ± 0.045	7.659ArgLeu: 7.659 ± 0.087	1.648ArgMet: 1.648 ± 0.036	1.964ArgAsn: 1.964 ± 0.04	2.761ArgPro: 2.761 ± 0.042	3.558ArgGln: 3.558 ± 0.058	4.701ArgArg: 4.701 ± 0.079	3.157ArgSer: 3.157 ± 0.046	2.734ArgThr: 2.734 ± 0.046	4.093ArgVal: 4.093 ± 0.056	1.165ArgTrp: 1.165 ± 0.035	2.464ArgTyr: 2.464 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	6.077SerAla: 6.077 ± 0.085	0.614SerCys: 0.614 ± 0.02	3.084SerAsp: 3.084 ± 0.043	3.149SerGlu: 3.149 ± 0.05	2.182SerPhe: 2.182 ± 0.042	5.659SerGly: 5.659 ± 0.075	1.325SerHis: 1.325 ± 0.027	2.821SerIle: 2.821 ± 0.048	1.812SerLys: 1.812 ± 0.041	6.722SerLeu: 6.722 ± 0.087	1.403SerMet: 1.403 ± 0.028	1.671SerAsn: 1.671 ± 0.04	2.782SerPro: 2.782 ± 0.048	2.358SerGln: 2.358 ± 0.045	3.934SerArg: 3.934 ± 0.066	3.382SerSer: 3.382 ± 0.064	2.822SerThr: 2.822 ± 0.056	4.353SerVal: 4.353 ± 0.062	0.995SerTrp: 0.995 ± 0.029	1.587SerTyr: 1.587 ± 0.036	0.001SerXaa: 0.001 ± 0.001
Thr	5.131ThrAla: 5.131 ± 0.077	0.538ThrCys: 0.538 ± 0.022	2.544ThrAsp: 2.544 ± 0.055	2.514ThrGlu: 2.514 ± 0.045	2.018ThrPhe: 2.018 ± 0.043	4.704ThrGly: 4.704 ± 0.066	1.119ThrHis: 1.119 ± 0.026	2.899ThrIle: 2.899 ± 0.053	1.335ThrLys: 1.335 ± 0.033	7.479ThrLeu: 7.479 ± 0.091	1.062ThrMet: 1.062 ± 0.029	1.418ThrAsn: 1.418 ± 0.037	3.259ThrPro: 3.259 ± 0.06	1.901ThrGln: 1.901 ± 0.041	3.289ThrArg: 3.289 ± 0.048	2.761ThrSer: 2.761 ± 0.057	2.658ThrThr: 2.658 ± 0.06	3.798ThrVal: 3.798 ± 0.077	0.821ThrTrp: 0.821 ± 0.024	1.025ThrTyr: 1.025 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	7.055ValAla: 7.055 ± 0.08	0.767ValCys: 0.767 ± 0.024	3.705ValAsp: 3.705 ± 0.054	3.96ValGlu: 3.96 ± 0.058	2.37ValPhe: 2.37 ± 0.044	4.848ValGly: 4.848 ± 0.076	1.243ValHis: 1.243 ± 0.029	4.201ValIle: 4.201 ± 0.063	3.103ValLys: 3.103 ± 0.056	7.029ValLeu: 7.029 ± 0.071	2.057ValMet: 2.057 ± 0.043	2.796ValAsn: 2.796 ± 0.041	3.048ValPro: 3.048 ± 0.042	2.302ValGln: 2.302 ± 0.04	4.055ValArg: 4.055 ± 0.058	4.569ValSer: 4.569 ± 0.066	4.248ValThr: 4.248 ± 0.069	5.36ValVal: 5.36 ± 0.074	1.008ValTrp: 1.008 ± 0.027	1.638ValTyr: 1.638 ± 0.037	0.001ValXaa: 0.001 ± 0.001
Trp	1.013TrpAla: 1.013 ± 0.026	0.195TrpCys: 0.195 ± 0.012	0.618TrpAsp: 0.618 ± 0.022	0.662TrpGlu: 0.662 ± 0.024	0.701TrpPhe: 0.701 ± 0.027	0.94TrpGly: 0.94 ± 0.029	0.446TrpHis: 0.446 ± 0.021	0.747TrpIle: 0.747 ± 0.025	0.536TrpLys: 0.536 ± 0.019	2.499TrpLeu: 2.499 ± 0.054	0.43TrpMet: 0.43 ± 0.02	0.465TrpAsn: 0.465 ± 0.019	0.74TrpPro: 0.74 ± 0.029	1.161TrpGln: 1.161 ± 0.03	1.254TrpArg: 1.254 ± 0.032	0.877TrpSer: 0.877 ± 0.029	0.575TrpThr: 0.575 ± 0.02	0.899TrpVal: 0.899 ± 0.028	0.243TrpTrp: 0.243 ± 0.014	0.452TrpTyr: 0.452 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.542TyrAla: 2.542 ± 0.05	0.368TyrCys: 0.368 ± 0.019	1.5TyrAsp: 1.5 ± 0.037	1.249TyrGlu: 1.249 ± 0.031	1.167TyrPhe: 1.167 ± 0.034	2.295TyrGly: 2.295 ± 0.048	0.685TyrHis: 0.685 ± 0.023	1.423TyrIle: 1.423 ± 0.033	0.985TyrLys: 0.985 ± 0.035	3.004TyrLeu: 3.004 ± 0.053	0.574TyrMet: 0.574 ± 0.022	0.984TyrAsn: 0.984 ± 0.031	1.335TyrPro: 1.335 ± 0.03	1.422TyrGln: 1.422 ± 0.034	2.034TyrArg: 2.034 ± 0.039	1.716TyrSer: 1.716 ± 0.039	1.452TyrThr: 1.452 ± 0.038	1.671TyrVal: 1.671 ± 0.039	0.419TyrTrp: 0.419 ± 0.019	0.923TyrTyr: 0.923 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4335 proteins (1333019 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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