Beta vulgaris subsp. vulgaris (Beet)
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7611 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0J8DSF5|A0A0J8DSF5_BETVV Uncharacterized protein OS=Beta vulgaris subsp. vulgaris OX=3555 GN=BVRB_029180 PE=4 SV=1
MM1 pKa = 7.29 NKK3 pKa = 9.91 SLALALGALMVASPALAQEE22 pKa = 4.12 EE23 pKa = 4.47 AVLNVYY29 pKa = 9.34 NWSDD33 pKa = 4.06 YY34 pKa = 10.51 IAEE37 pKa = 3.92 DD38 pKa = 3.95 TIANFEE44 pKa = 4.08 AATGIKK50 pKa = 10.12 VNYY53 pKa = 9.54 DD54 pKa = 3.15 VYY56 pKa = 11.54 DD57 pKa = 3.63 NNEE60 pKa = 3.78 IVDD63 pKa = 4.06 AKK65 pKa = 10.85 LLAGSSGYY73 pKa = 10.79 DD74 pKa = 2.68 IVVPSGNFLEE84 pKa = 4.48 RR85 pKa = 11.84 QIQAGLILPLDD96 pKa = 3.78 KK97 pKa = 11.27 SKK99 pKa = 9.84 LTNLGNLDD107 pKa = 4.01 PAVMATAAAQDD118 pKa = 3.86 PDD120 pKa = 3.72 NAHH123 pKa = 6.26 GVPYY127 pKa = 9.78 MINTIGYY134 pKa = 8.36 GYY136 pKa = 10.71 NVAKK140 pKa = 9.42 VTEE143 pKa = 4.21 ILGADD148 pKa = 3.73 APVDD152 pKa = 3.35 SWDD155 pKa = 5.1 LIFKK159 pKa = 10.5 PEE161 pKa = 3.85 FAEE164 pKa = 4.25 KK165 pKa = 10.56 LSACGISLLDD175 pKa = 3.71 SPSEE179 pKa = 4.09 VMGIALNYY187 pKa = 10.26 LGLDD191 pKa = 3.36 ANSEE195 pKa = 4.24 SEE197 pKa = 4.04 EE198 pKa = 4.07 DD199 pKa = 3.33 LAKK202 pKa = 10.78 AEE204 pKa = 4.11 EE205 pKa = 5.17 LINAIKK211 pKa = 10.48 PFIRR215 pKa = 11.84 YY216 pKa = 7.94 FNSSQYY222 pKa = 10.49 IDD224 pKa = 4.31 DD225 pKa = 4.69 LGNGEE230 pKa = 4.2 TCVALGYY237 pKa = 10.59 SGDD240 pKa = 3.41 IFIAADD246 pKa = 3.15 AA247 pKa = 4.59
Molecular weight: 26.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.859
IPC_protein 3.846
Toseland 3.63
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.668
Grimsley 3.541
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.681
EMBOSS 3.757
Sillero 3.961
Patrickios 1.138
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.852
Protein with the highest isoelectric point:
>tr|A0A0J7YMS7|A0A0J7YMS7_BETVV Serine/threonine-protein phosphatase 2A activator (Fragment) OS=Beta vulgaris subsp. vulgaris OX=3555 GN=BVRB_041550 PE=3 SV=1
SS1 pKa = 6.84 TIFTARR7 pKa = 11.84 RR8 pKa = 11.84 PDD10 pKa = 3.03 SMLQRR15 pKa = 11.84 RR16 pKa = 11.84 IFTARR21 pKa = 11.84 RR22 pKa = 11.84 PFVRR26 pKa = 11.84 RR27 pKa = 11.84 SNRR30 pKa = 11.84 LRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 SSRR37 pKa = 11.84 LRR39 pKa = 11.84 RR40 pKa = 11.84 FFRR43 pKa = 11.84 WSFQQATSSFRR54 pKa = 11.84 SFSFQQAAAEE64 pKa = 4.14 FRR66 pKa = 11.84 SSFVGG71 pKa = 3.44
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.428
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.93
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.157
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7157
454
7611
2194387
8
4316
288.3
32.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.921 ± 0.033
1.871 ± 0.016
5.514 ± 0.027
6.307 ± 0.034
4.049 ± 0.021
6.254 ± 0.043
2.441 ± 0.017
5.387 ± 0.026
5.893 ± 0.029
9.634 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.012
4.479 ± 0.023
4.963 ± 0.035
3.892 ± 0.027
5.461 ± 0.029
8.986 ± 0.038
4.985 ± 0.02
6.541 ± 0.023
1.215 ± 0.013
2.767 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here