Digitaria streak virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q67568|Q67568_9GEMI Replication-associated protein OS=Digitaria streak virus OX=10837 PE=3 SV=1
MM1 pKa = 7.4AANRR5 pKa = 11.84SFRR8 pKa = 11.84HH9 pKa = 5.38RR10 pKa = 11.84NANTFLTYY18 pKa = 10.33SKK20 pKa = 10.32CDD22 pKa = 3.45HH23 pKa = 6.64SPQLIADD30 pKa = 4.38HH31 pKa = 6.87LWDD34 pKa = 5.57LLKK37 pKa = 10.61SWNPIYY43 pKa = 10.48ILVASEE49 pKa = 3.78HH50 pKa = 6.43HH51 pKa = 6.98ADD53 pKa = 3.99GSLHH57 pKa = 5.62SHH59 pKa = 6.91ALVQTEE65 pKa = 4.2KK66 pKa = 10.77QVNTTNQRR74 pKa = 11.84FFDD77 pKa = 3.28ILEE80 pKa = 3.86FHH82 pKa = 7.37PNIQSAKK89 pKa = 9.09SVNKK93 pKa = 9.47VRR95 pKa = 11.84TYY97 pKa = 10.19ILKK100 pKa = 10.23NPVEE104 pKa = 4.16KK105 pKa = 10.6FEE107 pKa = 5.15RR108 pKa = 11.84GTFVPRR114 pKa = 11.84KK115 pKa = 9.73SPFLGEE121 pKa = 4.18SSSSEE126 pKa = 3.9KK127 pKa = 10.51KK128 pKa = 9.9HH129 pKa = 6.58NKK131 pKa = 9.2DD132 pKa = 3.28DD133 pKa = 3.69VMRR136 pKa = 11.84DD137 pKa = 3.6IIDD140 pKa = 3.68HH141 pKa = 5.73ATSKK145 pKa = 11.07EE146 pKa = 4.22EE147 pKa = 3.96YY148 pKa = 10.48LSMVQKK154 pKa = 10.83ALPYY158 pKa = 10.55DD159 pKa = 3.43WATKK163 pKa = 10.42LSYY166 pKa = 10.68FEE168 pKa = 4.67YY169 pKa = 10.61SADD172 pKa = 3.43RR173 pKa = 11.84LFPVEE178 pKa = 4.48AAPFINPHH186 pKa = 6.56PPSEE190 pKa = 4.54PDD192 pKa = 3.58LLCQEE197 pKa = 5.49TITDD201 pKa = 3.79WLQNDD206 pKa = 4.6LFQVSAEE213 pKa = 4.37AYY215 pKa = 9.48CLLNPTCYY223 pKa = 9.7TRR225 pKa = 11.84EE226 pKa = 3.8EE227 pKa = 4.77AISDD231 pKa = 4.17LQWMSDD237 pKa = 3.63YY238 pKa = 10.39TRR240 pKa = 11.84SRR242 pKa = 11.84MGSEE246 pKa = 3.85NAASTSSVQQEE257 pKa = 4.44LEE259 pKa = 4.14SLLGPEE265 pKa = 4.28AA266 pKa = 4.5

Molecular weight: 30.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q67567|Q67567_9GEMI Uncharacterized protein (Fragment) OS=Digitaria streak virus OX=10837 PE=4 SV=1
MM1 pKa = 7.26SSSMKK6 pKa = 10.25RR7 pKa = 11.84KK8 pKa = 8.92RR9 pKa = 11.84TDD11 pKa = 2.87EE12 pKa = 4.32GSGSRR17 pKa = 11.84RR18 pKa = 11.84FTKK21 pKa = 10.25KK22 pKa = 9.81KK23 pKa = 9.65SAAKK27 pKa = 10.19GRR29 pKa = 11.84TSSSRR34 pKa = 11.84AIRR37 pKa = 11.84PALQIQSFVSAGAATIAVPTGGVCHH62 pKa = 7.27LLSSYY67 pKa = 11.26SRR69 pKa = 11.84GSGEE73 pKa = 4.1GDD75 pKa = 2.58RR76 pKa = 11.84HH77 pKa = 5.17TNEE80 pKa = 3.68TVTYY84 pKa = 9.43KK85 pKa = 10.89AAFDD89 pKa = 3.69YY90 pKa = 10.99HH91 pKa = 7.06FSANAGPCAYY101 pKa = 10.34SSIGVGVLWLVYY113 pKa = 9.84DD114 pKa = 4.52AQPSGQVPAVTDD126 pKa = 3.76IFPHH130 pKa = 6.35DD131 pKa = 3.64TTLQSFPYY139 pKa = 7.3TWKK142 pKa = 10.2VGRR145 pKa = 11.84EE146 pKa = 3.83VCHH149 pKa = 6.21RR150 pKa = 11.84FVVKK154 pKa = 10.44RR155 pKa = 11.84RR156 pKa = 11.84WCFTMEE162 pKa = 3.97TNGRR166 pKa = 11.84IGSDD170 pKa = 3.39KK171 pKa = 10.7PPSNAVWPPCKK182 pKa = 9.78RR183 pKa = 11.84SIYY186 pKa = 8.45FHH188 pKa = 7.11KK189 pKa = 10.18FATGLGVKK197 pKa = 7.95TEE199 pKa = 4.21WKK201 pKa = 8.12NTTDD205 pKa = 3.62GGVGSIKK212 pKa = 10.37KK213 pKa = 9.07GALYY217 pKa = 9.47FVIAPGSGIDD227 pKa = 3.5FTLFGTCRR235 pKa = 11.84MYY237 pKa = 10.82FKK239 pKa = 10.94SVGNQQ244 pKa = 2.8

Molecular weight: 26.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

767

109

266

191.8

21.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.953 ± 0.271

1.956 ± 0.228

4.954 ± 0.804

5.476 ± 1.221

4.433 ± 0.587

5.867 ± 2.101

2.608 ± 0.671

4.433 ± 0.245

5.737 ± 0.882

7.301 ± 1.525

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.695 ± 0.162

3.781 ± 0.831

5.997 ± 0.623

3.781 ± 0.533

5.606 ± 0.643

9.648 ± 0.732

6.389 ± 0.686

6.128 ± 0.858

2.347 ± 0.289

3.911 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski