Cyanophage SS120-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Banchanvirus; Prochlorococcus virus SS120-1

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1UGV5|M1UGV5_9CAUD Uncharacterized protein OS=Cyanophage SS120-1 OX=616674 GN=CYYG_00051 PE=4 SV=1
MM1 pKa = 6.87KK2 pKa = 9.67TNHH5 pKa = 6.76ILLDD9 pKa = 4.73LIDD12 pKa = 5.27DD13 pKa = 4.07YY14 pKa = 11.54VVRR17 pKa = 11.84LEE19 pKa = 4.23DD20 pKa = 3.83DD21 pKa = 4.49GYY23 pKa = 10.41PLVEE27 pKa = 4.43VLDD30 pKa = 4.13ALIEE34 pKa = 4.25YY35 pKa = 10.63ADD37 pKa = 3.87VARR40 pKa = 11.84DD41 pKa = 3.49IYY43 pKa = 10.78FSNAPRR49 pKa = 11.84DD50 pKa = 3.69IYY52 pKa = 11.56GDD54 pKa = 3.4

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1TVS4|M1TVS4_9CAUD Uncharacterized protein OS=Cyanophage SS120-1 OX=616674 GN=CYYG_00022 PE=4 SV=1
MM1 pKa = 7.63ASSKK5 pKa = 9.1TSKK8 pKa = 10.22FYY10 pKa = 11.2KK11 pKa = 10.2KK12 pKa = 10.16NPKK15 pKa = 9.54SAAKK19 pKa = 9.83RR20 pKa = 11.84RR21 pKa = 11.84AYY23 pKa = 10.34DD24 pKa = 3.05RR25 pKa = 11.84AYY27 pKa = 10.04KK28 pKa = 10.24KK29 pKa = 10.36RR30 pKa = 11.84EE31 pKa = 3.92KK32 pKa = 10.91GSLASGAKK40 pKa = 9.53KK41 pKa = 10.09KK42 pKa = 10.13RR43 pKa = 11.84LKK45 pKa = 10.66RR46 pKa = 11.84ADD48 pKa = 3.36SKK50 pKa = 11.05RR51 pKa = 11.84WAEE54 pKa = 3.95RR55 pKa = 11.84KK56 pKa = 9.44RR57 pKa = 11.84RR58 pKa = 11.84GIAGKK63 pKa = 9.67GGKK66 pKa = 9.59DD67 pKa = 2.91ISHH70 pKa = 6.54TKK72 pKa = 9.87SGRR75 pKa = 11.84TVLEE79 pKa = 3.93SRR81 pKa = 11.84SKK83 pKa = 10.56NRR85 pKa = 11.84ARR87 pKa = 11.84NGKK90 pKa = 8.53NKK92 pKa = 10.19KK93 pKa = 7.15STKK96 pKa = 9.73KK97 pKa = 10.57

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

14383

48

1807

276.6

30.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.593 ± 0.293

0.953 ± 0.185

6.299 ± 0.185

6.41 ± 0.44

3.636 ± 0.197

7.286 ± 0.4

1.662 ± 0.171

5.555 ± 0.201

6.271 ± 0.489

8.434 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.571 ± 0.122

5.277 ± 0.339

4.123 ± 0.281

4.074 ± 0.305

4.63 ± 0.29

7.3 ± 0.339

7.154 ± 0.593

5.764 ± 0.269

1.46 ± 0.104

3.546 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski