Amino acid dipepetide frequency for Cyanophage SS120-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.135AlaAla: 8.135 ± 1.228
0.556AlaCys: 0.556 ± 0.178
5.98AlaAsp: 5.98 ± 0.592
4.172AlaGlu: 4.172 ± 0.44
3.755AlaPhe: 3.755 ± 0.481
6.675AlaGly: 6.675 ± 1.047
1.113AlaHis: 1.113 ± 0.32
5.076AlaIle: 5.076 ± 0.519
4.728AlaLys: 4.728 ± 0.612
7.231AlaLeu: 7.231 ± 0.586
1.391AlaMet: 1.391 ± 0.328
5.563AlaAsn: 5.563 ± 0.84
3.129AlaPro: 3.129 ± 0.534
3.824AlaGln: 3.824 ± 0.625
4.311AlaArg: 4.311 ± 0.661
6.466AlaSer: 6.466 ± 0.723
6.466AlaThr: 6.466 ± 0.817
4.033AlaVal: 4.033 ± 0.48
0.904AlaTrp: 0.904 ± 0.261
2.434AlaTyr: 2.434 ± 0.386
0.0AlaXaa: 0.0 ± 0.0
Cys
0.834CysAla: 0.834 ± 0.224
0.278CysCys: 0.278 ± 0.144
0.278CysAsp: 0.278 ± 0.145
0.487CysGlu: 0.487 ± 0.365
0.556CysPhe: 0.556 ± 0.19
0.626CysGly: 0.626 ± 0.28
0.209CysHis: 0.209 ± 0.124
0.487CysIle: 0.487 ± 0.178
0.834CysLys: 0.834 ± 0.281
1.043CysLeu: 1.043 ± 0.342
0.139CysMet: 0.139 ± 0.154
0.209CysAsn: 0.209 ± 0.119
0.417CysPro: 0.417 ± 0.191
0.278CysGln: 0.278 ± 0.133
0.556CysArg: 0.556 ± 0.229
0.695CysSer: 0.695 ± 0.223
0.626CysThr: 0.626 ± 0.25
0.626CysVal: 0.626 ± 0.202
0.07CysTrp: 0.07 ± 0.074
0.278CysTyr: 0.278 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
5.98AspAla: 5.98 ± 0.554
0.556AspCys: 0.556 ± 0.274
3.685AspAsp: 3.685 ± 0.642
4.867AspGlu: 4.867 ± 0.674
2.434AspPhe: 2.434 ± 0.357
4.659AspGly: 4.659 ± 0.627
1.113AspHis: 1.113 ± 0.259
4.589AspIle: 4.589 ± 0.447
3.198AspLys: 3.198 ± 0.487
5.493AspLeu: 5.493 ± 0.589
0.695AspMet: 0.695 ± 0.262
2.92AspAsn: 2.92 ± 0.573
2.434AspPro: 2.434 ± 0.531
2.434AspGln: 2.434 ± 0.473
3.059AspArg: 3.059 ± 0.405
4.311AspSer: 4.311 ± 0.538
3.546AspThr: 3.546 ± 0.683
3.198AspVal: 3.198 ± 0.342
1.113AspTrp: 1.113 ± 0.224
2.642AspTyr: 2.642 ± 0.378
0.0AspXaa: 0.0 ± 0.0
Glu
5.771GluAla: 5.771 ± 0.645
0.487GluCys: 0.487 ± 0.197
3.755GluAsp: 3.755 ± 0.584
5.284GluGlu: 5.284 ± 0.934
2.295GluPhe: 2.295 ± 0.517
4.38GluGly: 4.38 ± 0.595
0.904GluHis: 0.904 ± 0.255
3.824GluIle: 3.824 ± 0.593
3.546GluLys: 3.546 ± 0.475
7.301GluLeu: 7.301 ± 0.993
1.182GluMet: 1.182 ± 0.289
2.364GluAsn: 2.364 ± 0.354
2.086GluPro: 2.086 ± 0.427
2.364GluGln: 2.364 ± 0.467
2.503GluArg: 2.503 ± 0.428
3.616GluSer: 3.616 ± 0.492
4.867GluThr: 4.867 ± 0.778
4.311GluVal: 4.311 ± 0.589
1.113GluTrp: 1.113 ± 0.311
2.155GluTyr: 2.155 ± 0.331
0.0GluXaa: 0.0 ± 0.0
Phe
1.808PheAla: 1.808 ± 0.371
0.626PheCys: 0.626 ± 0.214
2.712PheAsp: 2.712 ± 0.491
2.155PheGlu: 2.155 ± 0.325
1.669PhePhe: 1.669 ± 0.341
2.225PheGly: 2.225 ± 0.399
0.765PheHis: 0.765 ± 0.233
1.877PheIle: 1.877 ± 0.253
2.225PheLys: 2.225 ± 0.359
3.059PheLeu: 3.059 ± 0.583
0.695PheMet: 0.695 ± 0.18
1.877PheAsn: 1.877 ± 0.455
2.016PhePro: 2.016 ± 0.458
1.738PheGln: 1.738 ± 0.449
2.016PheArg: 2.016 ± 0.377
2.016PheSer: 2.016 ± 0.449
2.642PheThr: 2.642 ± 0.334
2.573PheVal: 2.573 ± 0.328
0.487PheTrp: 0.487 ± 0.175
1.182PheTyr: 1.182 ± 0.242
0.0PheXaa: 0.0 ± 0.0
Gly
6.327GlyAla: 6.327 ± 0.794
0.487GlyCys: 0.487 ± 0.19
4.798GlyAsp: 4.798 ± 0.654
3.546GlyGlu: 3.546 ± 0.676
3.407GlyPhe: 3.407 ± 0.698
5.702GlyGly: 5.702 ± 0.822
1.252GlyHis: 1.252 ± 0.261
4.589GlyIle: 4.589 ± 0.663
4.102GlyLys: 4.102 ± 0.58
5.841GlyLeu: 5.841 ± 0.914
0.765GlyMet: 0.765 ± 0.21
3.824GlyAsn: 3.824 ± 0.751
2.086GlyPro: 2.086 ± 0.389
2.92GlyGln: 2.92 ± 0.491
2.92GlyArg: 2.92 ± 0.474
5.91GlySer: 5.91 ± 1.163
6.258GlyThr: 6.258 ± 1.078
4.033GlyVal: 4.033 ± 0.631
1.113GlyTrp: 1.113 ± 0.308
2.99GlyTyr: 2.99 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
1.182HisAla: 1.182 ± 0.272
0.278HisCys: 0.278 ± 0.139
1.252HisAsp: 1.252 ± 0.251
0.973HisGlu: 0.973 ± 0.299
0.556HisPhe: 0.556 ± 0.243
1.321HisGly: 1.321 ± 0.359
0.278HisHis: 0.278 ± 0.147
0.487HisIle: 0.487 ± 0.184
0.973HisLys: 0.973 ± 0.26
1.808HisLeu: 1.808 ± 0.486
0.487HisMet: 0.487 ± 0.175
0.556HisAsn: 0.556 ± 0.17
0.695HisPro: 0.695 ± 0.179
0.626HisGln: 0.626 ± 0.242
0.626HisArg: 0.626 ± 0.22
1.391HisSer: 1.391 ± 0.364
1.321HisThr: 1.321 ± 0.344
0.765HisVal: 0.765 ± 0.256
0.07HisTrp: 0.07 ± 0.071
0.973HisTyr: 0.973 ± 0.307
0.0HisXaa: 0.0 ± 0.0
Ile
5.145IleAla: 5.145 ± 0.556
0.209IleCys: 0.209 ± 0.131
4.241IleAsp: 4.241 ± 0.636
4.52IleGlu: 4.52 ± 0.758
1.391IlePhe: 1.391 ± 0.279
3.129IleGly: 3.129 ± 0.557
0.973IleHis: 0.973 ± 0.229
2.642IleIle: 2.642 ± 0.485
4.033IleLys: 4.033 ± 0.782
4.311IleLeu: 4.311 ± 0.53
1.113IleMet: 1.113 ± 0.276
3.129IleAsn: 3.129 ± 0.451
2.781IlePro: 2.781 ± 0.504
2.503IleGln: 2.503 ± 0.587
2.642IleArg: 2.642 ± 0.461
3.059IleSer: 3.059 ± 0.489
3.685IleThr: 3.685 ± 0.584
2.99IleVal: 2.99 ± 0.447
0.556IleTrp: 0.556 ± 0.207
2.503IleTyr: 2.503 ± 0.415
0.0IleXaa: 0.0 ± 0.0
Lys
5.98LysAla: 5.98 ± 0.657
0.348LysCys: 0.348 ± 0.154
4.033LysAsp: 4.033 ± 0.626
4.728LysGlu: 4.728 ± 0.569
2.016LysPhe: 2.016 ± 0.363
4.867LysGly: 4.867 ± 0.758
1.182LysHis: 1.182 ± 0.316
3.268LysIle: 3.268 ± 0.632
3.963LysLys: 3.963 ± 0.844
5.354LysLeu: 5.354 ± 0.612
1.252LysMet: 1.252 ± 0.304
2.434LysAsn: 2.434 ± 0.432
2.99LysPro: 2.99 ± 0.543
2.086LysGln: 2.086 ± 0.426
3.546LysArg: 3.546 ± 0.697
4.45LysSer: 4.45 ± 0.528
4.241LysThr: 4.241 ± 0.528
2.712LysVal: 2.712 ± 0.362
1.113LysTrp: 1.113 ± 0.251
2.155LysTyr: 2.155 ± 0.391
0.0LysXaa: 0.0 ± 0.0
Leu
6.953LeuAla: 6.953 ± 0.929
0.487LeuCys: 0.487 ± 0.184
5.771LeuAsp: 5.771 ± 0.721
6.258LeuGlu: 6.258 ± 0.742
2.503LeuPhe: 2.503 ± 0.451
5.423LeuGly: 5.423 ± 0.449
1.391LeuHis: 1.391 ± 0.292
4.52LeuIle: 4.52 ± 0.72
6.049LeuLys: 6.049 ± 0.899
5.91LeuLeu: 5.91 ± 0.619
1.321LeuMet: 1.321 ± 0.332
5.215LeuAsn: 5.215 ± 0.448
3.616LeuPro: 3.616 ± 0.769
3.894LeuGln: 3.894 ± 0.373
4.45LeuArg: 4.45 ± 0.605
6.675LeuSer: 6.675 ± 0.671
5.632LeuThr: 5.632 ± 0.589
5.076LeuVal: 5.076 ± 0.752
0.695LeuTrp: 0.695 ± 0.204
2.503LeuTyr: 2.503 ± 0.321
0.0LeuXaa: 0.0 ± 0.0
Met
1.669MetAla: 1.669 ± 0.329
0.348MetCys: 0.348 ± 0.209
0.695MetAsp: 0.695 ± 0.197
1.252MetGlu: 1.252 ± 0.357
0.556MetPhe: 0.556 ± 0.223
0.834MetGly: 0.834 ± 0.196
0.348MetHis: 0.348 ± 0.159
0.973MetIle: 0.973 ± 0.231
1.391MetLys: 1.391 ± 0.366
1.043MetLeu: 1.043 ± 0.293
0.209MetMet: 0.209 ± 0.145
0.626MetAsn: 0.626 ± 0.234
0.904MetPro: 0.904 ± 0.25
0.765MetGln: 0.765 ± 0.19
0.417MetArg: 0.417 ± 0.19
1.252MetSer: 1.252 ± 0.32
1.182MetThr: 1.182 ± 0.361
0.695MetVal: 0.695 ± 0.254
0.07MetTrp: 0.07 ± 0.068
0.487MetTyr: 0.487 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
4.52AsnAla: 4.52 ± 0.896
0.139AsnCys: 0.139 ± 0.095
3.198AsnAsp: 3.198 ± 0.456
2.573AsnGlu: 2.573 ± 0.48
2.155AsnPhe: 2.155 ± 0.373
4.798AsnGly: 4.798 ± 0.897
0.556AsnHis: 0.556 ± 0.234
2.295AsnIle: 2.295 ± 0.424
2.642AsnLys: 2.642 ± 0.497
4.102AsnLeu: 4.102 ± 0.602
0.348AsnMet: 0.348 ± 0.172
3.338AsnAsn: 3.338 ± 0.757
3.059AsnPro: 3.059 ± 0.396
2.92AsnGln: 2.92 ± 0.49
1.738AsnArg: 1.738 ± 0.377
3.755AsnSer: 3.755 ± 0.745
4.033AsnThr: 4.033 ± 0.766
3.477AsnVal: 3.477 ± 0.611
0.765AsnTrp: 0.765 ± 0.36
2.364AsnTyr: 2.364 ± 0.363
0.0AsnXaa: 0.0 ± 0.0
Pro
2.99ProAla: 2.99 ± 0.488
0.487ProCys: 0.487 ± 0.189
2.781ProAsp: 2.781 ± 0.517
3.407ProGlu: 3.407 ± 0.589
1.46ProPhe: 1.46 ± 0.33
2.016ProGly: 2.016 ± 0.401
0.904ProHis: 0.904 ± 0.278
2.503ProIle: 2.503 ± 0.423
2.295ProLys: 2.295 ± 0.665
2.92ProLeu: 2.92 ± 0.416
0.417ProMet: 0.417 ± 0.162
2.781ProAsn: 2.781 ± 0.441
1.947ProPro: 1.947 ± 0.568
1.599ProGln: 1.599 ± 0.398
1.252ProArg: 1.252 ± 0.369
3.198ProSer: 3.198 ± 0.416
2.781ProThr: 2.781 ± 0.387
2.92ProVal: 2.92 ± 0.468
0.834ProTrp: 0.834 ± 0.302
1.738ProTyr: 1.738 ± 0.389
0.0ProXaa: 0.0 ± 0.0
Gln
4.38GlnAla: 4.38 ± 0.844
0.348GlnCys: 0.348 ± 0.169
2.016GlnAsp: 2.016 ± 0.318
2.712GlnGlu: 2.712 ± 0.627
1.53GlnPhe: 1.53 ± 0.271
2.712GlnGly: 2.712 ± 0.456
0.834GlnHis: 0.834 ± 0.241
1.947GlnIle: 1.947 ± 0.453
2.781GlnLys: 2.781 ± 0.533
3.129GlnLeu: 3.129 ± 0.58
1.182GlnMet: 1.182 ± 0.343
1.877GlnAsn: 1.877 ± 0.315
1.599GlnPro: 1.599 ± 0.338
2.295GlnGln: 2.295 ± 0.767
1.669GlnArg: 1.669 ± 0.358
2.851GlnSer: 2.851 ± 0.499
2.364GlnThr: 2.364 ± 0.524
2.503GlnVal: 2.503 ± 0.601
0.765GlnTrp: 0.765 ± 0.245
1.252GlnTyr: 1.252 ± 0.458
0.0GlnXaa: 0.0 ± 0.0
Arg
3.685ArgAla: 3.685 ± 0.624
0.695ArgCys: 0.695 ± 0.235
2.155ArgAsp: 2.155 ± 0.429
2.712ArgGlu: 2.712 ± 0.532
1.738ArgPhe: 1.738 ± 0.38
2.434ArgGly: 2.434 ± 0.381
1.043ArgHis: 1.043 ± 0.307
2.434ArgIle: 2.434 ± 0.413
4.589ArgLys: 4.589 ± 0.674
4.241ArgLeu: 4.241 ± 0.454
1.182ArgMet: 1.182 ± 0.264
1.738ArgAsn: 1.738 ± 0.391
1.947ArgPro: 1.947 ± 0.47
2.295ArgGln: 2.295 ± 0.316
2.016ArgArg: 2.016 ± 0.445
4.033ArgSer: 4.033 ± 0.563
2.225ArgThr: 2.225 ± 0.371
2.781ArgVal: 2.781 ± 0.499
0.626ArgTrp: 0.626 ± 0.216
1.738ArgTyr: 1.738 ± 0.276
0.0ArgXaa: 0.0 ± 0.0
Ser
6.536SerAla: 6.536 ± 0.843
0.904SerCys: 0.904 ± 0.405
4.241SerAsp: 4.241 ± 0.614
3.198SerGlu: 3.198 ± 0.409
2.155SerPhe: 2.155 ± 0.323
6.953SerGly: 6.953 ± 1.133
1.252SerHis: 1.252 ± 0.287
4.937SerIle: 4.937 ± 0.742
4.52SerLys: 4.52 ± 0.582
6.397SerLeu: 6.397 ± 0.681
0.834SerMet: 0.834 ± 0.257
4.45SerAsn: 4.45 ± 0.687
2.155SerPro: 2.155 ± 0.499
2.225SerGln: 2.225 ± 0.455
3.963SerArg: 3.963 ± 0.556
5.076SerSer: 5.076 ± 0.683
4.937SerThr: 4.937 ± 0.895
4.241SerVal: 4.241 ± 0.702
1.599SerTrp: 1.599 ± 0.418
2.434SerTyr: 2.434 ± 0.32
0.0SerXaa: 0.0 ± 0.0
Thr
5.98ThrAla: 5.98 ± 0.852
0.765ThrCys: 0.765 ± 0.276
3.685ThrAsp: 3.685 ± 0.637
4.798ThrGlu: 4.798 ± 0.668
2.573ThrPhe: 2.573 ± 0.506
6.814ThrGly: 6.814 ± 0.883
0.695ThrHis: 0.695 ± 0.236
4.102ThrIle: 4.102 ± 0.724
3.546ThrLys: 3.546 ± 0.47
6.188ThrLeu: 6.188 ± 1.178
0.695ThrMet: 0.695 ± 0.178
4.102ThrAsn: 4.102 ± 0.678
4.033ThrPro: 4.033 ± 0.496
2.434ThrGln: 2.434 ± 0.423
2.295ThrArg: 2.295 ± 0.382
5.006ThrSer: 5.006 ± 0.703
6.049ThrThr: 6.049 ± 0.962
4.867ThrVal: 4.867 ± 1.123
0.904ThrTrp: 0.904 ± 0.301
2.016ThrTyr: 2.016 ± 0.396
0.0ThrXaa: 0.0 ± 0.0
Val
5.145ValAla: 5.145 ± 0.595
0.765ValCys: 0.765 ± 0.329
4.311ValAsp: 4.311 ± 0.418
3.477ValGlu: 3.477 ± 0.43
1.877ValPhe: 1.877 ± 0.441
4.38ValGly: 4.38 ± 0.542
0.904ValHis: 0.904 ± 0.242
2.225ValIle: 2.225 ± 0.385
4.033ValLys: 4.033 ± 0.479
4.45ValLeu: 4.45 ± 0.697
0.904ValMet: 0.904 ± 0.254
3.407ValAsn: 3.407 ± 0.452
1.877ValPro: 1.877 ± 0.362
1.947ValGln: 1.947 ± 0.32
2.781ValArg: 2.781 ± 0.54
4.38ValSer: 4.38 ± 0.714
4.937ValThr: 4.937 ± 0.74
3.198ValVal: 3.198 ± 0.479
0.973ValTrp: 0.973 ± 0.347
1.669ValTyr: 1.669 ± 0.282
0.0ValXaa: 0.0 ± 0.0
Trp
1.182TrpAla: 1.182 ± 0.303
0.139TrpCys: 0.139 ± 0.106
1.043TrpAsp: 1.043 ± 0.295
1.113TrpGlu: 1.113 ± 0.323
0.348TrpPhe: 0.348 ± 0.126
0.904TrpGly: 0.904 ± 0.233
0.278TrpHis: 0.278 ± 0.126
0.695TrpIle: 0.695 ± 0.242
0.556TrpLys: 0.556 ± 0.163
1.669TrpLeu: 1.669 ± 0.367
0.278TrpMet: 0.278 ± 0.13
0.556TrpAsn: 0.556 ± 0.163
0.556TrpPro: 0.556 ± 0.229
0.487TrpGln: 0.487 ± 0.162
0.695TrpArg: 0.695 ± 0.158
1.043TrpSer: 1.043 ± 0.235
1.113TrpThr: 1.113 ± 0.306
1.113TrpVal: 1.113 ± 0.273
0.0TrpTrp: 0.0 ± 0.0
0.834TrpTyr: 0.834 ± 0.285
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.738TyrAla: 1.738 ± 0.32
0.626TyrCys: 0.626 ± 0.227
2.364TyrAsp: 2.364 ± 0.352
1.877TyrGlu: 1.877 ± 0.336
1.391TyrPhe: 1.391 ± 0.292
2.016TyrGly: 2.016 ± 0.358
0.626TyrHis: 0.626 ± 0.247
2.086TyrIle: 2.086 ± 0.371
2.712TyrLys: 2.712 ± 0.44
2.851TyrLeu: 2.851 ± 0.501
0.556TyrMet: 0.556 ± 0.174
1.808TyrAsn: 1.808 ± 0.325
0.904TyrPro: 0.904 ± 0.23
1.113TyrGln: 1.113 ± 0.278
2.851TyrArg: 2.851 ± 0.441
3.824TyrSer: 3.824 ± 0.478
2.642TyrThr: 2.642 ± 0.6
1.53TyrVal: 1.53 ± 0.343
0.834TyrTrp: 0.834 ± 0.248
1.113TyrTyr: 1.113 ± 0.298
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (14383 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski