Halobellus limi

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halobellus

Average proteome isoelectric point is 4.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3637 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6B7C3|A0A1H6B7C3_9EURY Catalase-peroxidase OS=Halobellus limi OX=699433 GN=katG PE=3 SV=1
MM1 pKa = 7.33NPEE4 pKa = 4.64DD5 pKa = 4.86LPTPGAEE12 pKa = 3.93VDD14 pKa = 4.12SVSPEE19 pKa = 3.75ALKK22 pKa = 10.59EE23 pKa = 3.97QIDD26 pKa = 3.71AGEE29 pKa = 4.59DD30 pKa = 3.3VTLLDD35 pKa = 3.82TRR37 pKa = 11.84MQSDD41 pKa = 4.04YY42 pKa = 11.33EE43 pKa = 4.07EE44 pKa = 3.93WHH46 pKa = 6.63IDD48 pKa = 3.3GDD50 pKa = 4.18NVTSINLPYY59 pKa = 10.59FHH61 pKa = 7.27FLEE64 pKa = 6.02DD65 pKa = 6.11DD66 pKa = 3.33IDD68 pKa = 6.16DD69 pKa = 4.64DD70 pKa = 4.64VLEE73 pKa = 4.2QVPADD78 pKa = 3.87RR79 pKa = 11.84EE80 pKa = 4.58VTVLCAKK87 pKa = 10.38GGASEE92 pKa = 4.54FVAGALKK99 pKa = 10.76ARR101 pKa = 11.84GYY103 pKa = 10.96DD104 pKa = 3.32VDD106 pKa = 3.83HH107 pKa = 7.26LEE109 pKa = 4.21NGMNGWAEE117 pKa = 3.54IYY119 pKa = 10.01EE120 pKa = 4.33RR121 pKa = 11.84YY122 pKa = 9.7EE123 pKa = 3.67VTDD126 pKa = 3.41YY127 pKa = 11.74DD128 pKa = 4.28GAGTLYY134 pKa = 10.35QYY136 pKa = 10.4QRR138 pKa = 11.84PSSGCLGYY146 pKa = 11.15LLVDD150 pKa = 3.88GGEE153 pKa = 4.14AAVVDD158 pKa = 4.38PLRR161 pKa = 11.84AFTDD165 pKa = 4.01RR166 pKa = 11.84YY167 pKa = 10.72LDD169 pKa = 4.04DD170 pKa = 4.5AAEE173 pKa = 4.41LDD175 pKa = 4.21AEE177 pKa = 4.29LTYY180 pKa = 11.39AFDD183 pKa = 3.63THH185 pKa = 6.62IHH187 pKa = 6.74ADD189 pKa = 3.7HH190 pKa = 6.95ISGVRR195 pKa = 11.84NLDD198 pKa = 3.37AEE200 pKa = 4.54GVEE203 pKa = 4.58GVIPAAAVDD212 pKa = 3.84RR213 pKa = 11.84GVTYY217 pKa = 10.96ADD219 pKa = 3.8EE220 pKa = 4.38LTTAEE225 pKa = 4.93DD226 pKa = 4.09GDD228 pKa = 4.24TFSVGDD234 pKa = 3.71ATVEE238 pKa = 4.34TVATPGHH245 pKa = 4.8TTGMTSYY252 pKa = 11.22LLDD255 pKa = 3.96EE256 pKa = 4.96SLLATT261 pKa = 4.61

Molecular weight: 28.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5WP69|A0A1H5WP69_9EURY Uncharacterized protein OS=Halobellus limi OX=699433 GN=DV707_01055 PE=4 SV=1
MM1 pKa = 7.83LLRR4 pKa = 11.84PKK6 pKa = 10.73DD7 pKa = 3.28RR8 pKa = 11.84TVRR11 pKa = 11.84MLRR14 pKa = 11.84QFPARR19 pKa = 11.84KK20 pKa = 8.54PLQASKK26 pKa = 10.51LAAVLAVLLFGTLGFFRR43 pKa = 11.84LVPDD47 pKa = 3.85RR48 pKa = 11.84QLTALLVVPFAGFALALVVLGEE70 pKa = 4.04VLVAGFRR77 pKa = 11.84LVSADD82 pKa = 3.54APASDD87 pKa = 5.24RR88 pKa = 11.84IDD90 pKa = 3.82DD91 pKa = 3.93RR92 pKa = 11.84PVYY95 pKa = 7.88TTVRR99 pKa = 11.84VIEE102 pKa = 4.09AVAALVAVVGVAGTIASVPSDD123 pKa = 3.99PPPGPGAIGLLFVAGGFGLLVLGATLVRR151 pKa = 11.84TAVEE155 pKa = 4.74CYY157 pKa = 8.42HH158 pKa = 5.92TARR161 pKa = 11.84GG162 pKa = 3.57

Molecular weight: 16.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3637

0

3637

1069361

36

4263

294.0

31.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.9 ± 0.054

0.64 ± 0.013

8.291 ± 0.057

8.591 ± 0.064

3.36 ± 0.031

8.834 ± 0.044

1.89 ± 0.022

3.969 ± 0.037

1.789 ± 0.026

8.91 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.671 ± 0.018

2.257 ± 0.024

4.691 ± 0.03

2.294 ± 0.021

6.705 ± 0.044

5.793 ± 0.037

6.279 ± 0.048

9.295 ± 0.044

1.152 ± 0.016

2.689 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski