Halobellus limi
Average proteome isoelectric point is 4.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3637 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H6B7C3|A0A1H6B7C3_9EURY Catalase-peroxidase OS=Halobellus limi OX=699433 GN=katG PE=3 SV=1
MM1 pKa = 7.33 NPEE4 pKa = 4.64 DD5 pKa = 4.86 LPTPGAEE12 pKa = 3.93 VDD14 pKa = 4.12 SVSPEE19 pKa = 3.75 ALKK22 pKa = 10.59 EE23 pKa = 3.97 QIDD26 pKa = 3.71 AGEE29 pKa = 4.59 DD30 pKa = 3.3 VTLLDD35 pKa = 3.82 TRR37 pKa = 11.84 MQSDD41 pKa = 4.04 YY42 pKa = 11.33 EE43 pKa = 4.07 EE44 pKa = 3.93 WHH46 pKa = 6.63 IDD48 pKa = 3.3 GDD50 pKa = 4.18 NVTSINLPYY59 pKa = 10.59 FHH61 pKa = 7.27 FLEE64 pKa = 6.02 DD65 pKa = 6.11 DD66 pKa = 3.33 IDD68 pKa = 6.16 DD69 pKa = 4.64 DD70 pKa = 4.64 VLEE73 pKa = 4.2 QVPADD78 pKa = 3.87 RR79 pKa = 11.84 EE80 pKa = 4.58 VTVLCAKK87 pKa = 10.38 GGASEE92 pKa = 4.54 FVAGALKK99 pKa = 10.76 ARR101 pKa = 11.84 GYY103 pKa = 10.96 DD104 pKa = 3.32 VDD106 pKa = 3.83 HH107 pKa = 7.26 LEE109 pKa = 4.21 NGMNGWAEE117 pKa = 3.54 IYY119 pKa = 10.01 EE120 pKa = 4.33 RR121 pKa = 11.84 YY122 pKa = 9.7 EE123 pKa = 3.67 VTDD126 pKa = 3.41 YY127 pKa = 11.74 DD128 pKa = 4.28 GAGTLYY134 pKa = 10.35 QYY136 pKa = 10.4 QRR138 pKa = 11.84 PSSGCLGYY146 pKa = 11.15 LLVDD150 pKa = 3.88 GGEE153 pKa = 4.14 AAVVDD158 pKa = 4.38 PLRR161 pKa = 11.84 AFTDD165 pKa = 4.01 RR166 pKa = 11.84 YY167 pKa = 10.72 LDD169 pKa = 4.04 DD170 pKa = 4.5 AAEE173 pKa = 4.41 LDD175 pKa = 4.21 AEE177 pKa = 4.29 LTYY180 pKa = 11.39 AFDD183 pKa = 3.63 THH185 pKa = 6.62 IHH187 pKa = 6.74 ADD189 pKa = 3.7 HH190 pKa = 6.95 ISGVRR195 pKa = 11.84 NLDD198 pKa = 3.37 AEE200 pKa = 4.54 GVEE203 pKa = 4.58 GVIPAAAVDD212 pKa = 3.84 RR213 pKa = 11.84 GVTYY217 pKa = 10.96 ADD219 pKa = 3.8 EE220 pKa = 4.38 LTTAEE225 pKa = 4.93 DD226 pKa = 4.09 GDD228 pKa = 4.24 TFSVGDD234 pKa = 3.71 ATVEE238 pKa = 4.34 TVATPGHH245 pKa = 4.8 TTGMTSYY252 pKa = 11.22 LLDD255 pKa = 3.96 EE256 pKa = 4.96 SLLATT261 pKa = 4.61
Molecular weight: 28.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.554
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.884
Patrickios 0.985
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A1H5WP69|A0A1H5WP69_9EURY Uncharacterized protein OS=Halobellus limi OX=699433 GN=DV707_01055 PE=4 SV=1
MM1 pKa = 7.83 LLRR4 pKa = 11.84 PKK6 pKa = 10.73 DD7 pKa = 3.28 RR8 pKa = 11.84 TVRR11 pKa = 11.84 MLRR14 pKa = 11.84 QFPARR19 pKa = 11.84 KK20 pKa = 8.54 PLQASKK26 pKa = 10.51 LAAVLAVLLFGTLGFFRR43 pKa = 11.84 LVPDD47 pKa = 3.85 RR48 pKa = 11.84 QLTALLVVPFAGFALALVVLGEE70 pKa = 4.04 VLVAGFRR77 pKa = 11.84 LVSADD82 pKa = 3.54 APASDD87 pKa = 5.24 RR88 pKa = 11.84 IDD90 pKa = 3.82 DD91 pKa = 3.93 RR92 pKa = 11.84 PVYY95 pKa = 7.88 TTVRR99 pKa = 11.84 VIEE102 pKa = 4.09 AVAALVAVVGVAGTIASVPSDD123 pKa = 3.99 PPPGPGAIGLLFVAGGFGLLVLGATLVRR151 pKa = 11.84 TAVEE155 pKa = 4.74 CYY157 pKa = 8.42 HH158 pKa = 5.92 TARR161 pKa = 11.84 GG162 pKa = 3.57
Molecular weight: 16.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.502
IPC_protein 10.482
Toseland 10.584
ProMoST 10.379
Dawson 10.687
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.745
Grimsley 10.745
Solomon 10.847
Lehninger 10.804
Nozaki 10.57
DTASelect 10.438
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.628
Patrickios 10.526
IPC_peptide 10.847
IPC2_peptide 9.56
IPC2.peptide.svr19 8.636
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3637
0
3637
1069361
36
4263
294.0
31.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.9 ± 0.054
0.64 ± 0.013
8.291 ± 0.057
8.591 ± 0.064
3.36 ± 0.031
8.834 ± 0.044
1.89 ± 0.022
3.969 ± 0.037
1.789 ± 0.026
8.91 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.671 ± 0.018
2.257 ± 0.024
4.691 ± 0.03
2.294 ± 0.021
6.705 ± 0.044
5.793 ± 0.037
6.279 ± 0.048
9.295 ± 0.044
1.152 ± 0.016
2.689 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here