Moniliophthora perniciosa (strain FA553 / isolate CP02) (Witches -broom disease fungus) (Marasmius perniciosus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Marasmiaceae; Moniliophthora; Moniliophthora perniciosa

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13649 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E2LNG8|E2LNG8_MONPE Uncharacterized protein (Fragment) OS=Moniliophthora perniciosa (strain FA553 / isolate CP02) OX=554373 GN=MPER_08369 PE=4 SV=1
SS1 pKa = 7.61PIRR4 pKa = 11.84TIISNSGNLVHH15 pKa = 7.49IGQLYY20 pKa = 10.3FDD22 pKa = 5.22DD23 pKa = 5.02AFNDD27 pKa = 3.45QILALEE33 pKa = 4.93PYY35 pKa = 8.91TGNSNTRR42 pKa = 11.84TLNKK46 pKa = 10.11DD47 pKa = 3.0DD48 pKa = 6.06AYY50 pKa = 11.15LQEE53 pKa = 4.77QLEE56 pKa = 4.51DD57 pKa = 4.23DD58 pKa = 3.94NNAFIDD64 pKa = 3.92VQLLGEE70 pKa = 4.16TLADD74 pKa = 3.58GVLGYY79 pKa = 10.03ISKK82 pKa = 8.96LTINFVLKK90 pKa = 8.09TTIDD94 pKa = 3.45TFYY97 pKa = 10.51LAVGIDD103 pKa = 3.46STASYY108 pKa = 10.56GISNTNYY115 pKa = 10.4LNSTDD120 pKa = 3.67TDD122 pKa = 3.68SEE124 pKa = 4.74DD125 pKa = 3.41FVMVV129 pKa = 4.1

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2M2Y4|E2M2Y4_MONPE DUF1604 domain-containing protein (Fragment) OS=Moniliophthora perniciosa (strain FA553 / isolate CP02) OX=554373 GN=MPER_14461 PE=4 SV=1
FF1 pKa = 7.31KK2 pKa = 10.57RR3 pKa = 11.84VGPNAMRR10 pKa = 11.84GRR12 pKa = 11.84GIGTARR18 pKa = 11.84GRR20 pKa = 11.84ATIMRR25 pKa = 11.84ANARR29 pKa = 11.84RR30 pKa = 11.84GRR32 pKa = 11.84GVAPTAGRR40 pKa = 11.84GIRR43 pKa = 11.84RR44 pKa = 3.61

Molecular weight: 4.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13649

0

13649

2499614

15

1671

183.1

20.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.02 ± 0.023

1.242 ± 0.01

5.762 ± 0.022

6.245 ± 0.024

3.991 ± 0.017

6.712 ± 0.024

2.387 ± 0.011

5.369 ± 0.021

5.172 ± 0.022

9.138 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.22 ± 0.009

3.833 ± 0.015

5.663 ± 0.025

3.694 ± 0.017

5.775 ± 0.021

7.882 ± 0.024

5.981 ± 0.02

6.428 ± 0.019

1.557 ± 0.009

2.889 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski