Streptomyces sp. MMG1533

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8476 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M8TF94|A0A0M8TF94_9ACTN Benzoate transporter OS=Streptomyces sp. MMG1533 OX=1415546 GN=ADK57_43320 PE=4 SV=1
MM1 pKa = 7.39SRR3 pKa = 11.84LASAGAAAALIGLAAASPAMADD25 pKa = 3.55DD26 pKa = 5.52EE27 pKa = 4.98GPLWYY32 pKa = 10.37SNRR35 pKa = 11.84GTVTFNDD42 pKa = 4.27DD43 pKa = 3.28GDD45 pKa = 5.38DD46 pKa = 3.35ITVCDD51 pKa = 4.73KK52 pKa = 11.17LADD55 pKa = 4.34GYY57 pKa = 11.69GVTGYY62 pKa = 10.66LEE64 pKa = 4.47RR65 pKa = 11.84LNSSGTGLVWVIKK78 pKa = 10.66EE79 pKa = 4.21EE80 pKa = 4.53DD81 pKa = 3.87GGDD84 pKa = 3.69AGCDD88 pKa = 3.2RR89 pKa = 11.84SDD91 pKa = 3.88NGYY94 pKa = 11.46DD95 pKa = 3.21MIGNVSHH102 pKa = 6.86GLAICWNGNGNCVRR116 pKa = 11.84SNWYY120 pKa = 9.87FDD122 pKa = 3.49EE123 pKa = 4.5

Molecular weight: 12.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8TC50|A0A0M8TC50_9ACTN Sporulation protein OS=Streptomyces sp. MMG1533 OX=1415546 GN=ADK57_42785 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8476

0

8476

2742154

25

3659

323.5

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.197 ± 0.038

0.794 ± 0.008

6.059 ± 0.02

5.751 ± 0.029

2.794 ± 0.016

9.403 ± 0.027

2.288 ± 0.013

3.234 ± 0.017

2.276 ± 0.022

10.296 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.75 ± 0.011

1.868 ± 0.015

5.937 ± 0.023

2.815 ± 0.017

7.789 ± 0.029

5.169 ± 0.02

6.246 ± 0.025

8.587 ± 0.025

1.566 ± 0.011

2.182 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski