Amino acid dipepetide frequency for Streptomyces sp. MMG1533

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.057AlaAla: 20.057 ± 0.129
1.074AlaCys: 1.074 ± 0.02
8.254AlaAsp: 8.254 ± 0.062
8.706AlaGlu: 8.706 ± 0.074
3.526AlaPhe: 3.526 ± 0.037
12.53AlaGly: 12.53 ± 0.072
2.795AlaHis: 2.795 ± 0.034
3.534AlaIle: 3.534 ± 0.041
2.944AlaLys: 2.944 ± 0.047
14.057AlaLeu: 14.057 ± 0.087
2.446AlaMet: 2.446 ± 0.032
1.98AlaAsn: 1.98 ± 0.026
6.659AlaPro: 6.659 ± 0.069
3.709AlaGln: 3.709 ± 0.048
9.828AlaArg: 9.828 ± 0.081
6.059AlaSer: 6.059 ± 0.053
6.997AlaThr: 6.997 ± 0.06
12.115AlaVal: 12.115 ± 0.074
1.876AlaTrp: 1.876 ± 0.029
2.826AlaTyr: 2.826 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.054CysAla: 1.054 ± 0.017
0.095CysCys: 0.095 ± 0.007
0.481CysAsp: 0.481 ± 0.015
0.435CysGlu: 0.435 ± 0.014
0.223CysPhe: 0.223 ± 0.01
0.961CysGly: 0.961 ± 0.02
0.197CysHis: 0.197 ± 0.008
0.18CysIle: 0.18 ± 0.008
0.122CysLys: 0.122 ± 0.006
0.762CysLeu: 0.762 ± 0.017
0.117CysMet: 0.117 ± 0.007
0.139CysAsn: 0.139 ± 0.008
0.489CysPro: 0.489 ± 0.016
0.172CysGln: 0.172 ± 0.008
0.576CysArg: 0.576 ± 0.016
0.437CysSer: 0.437 ± 0.013
0.541CysThr: 0.541 ± 0.015
0.673CysVal: 0.673 ± 0.015
0.127CysTrp: 0.127 ± 0.007
0.156CysTyr: 0.156 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.498AspAla: 7.498 ± 0.056
0.433AspCys: 0.433 ± 0.014
3.734AspAsp: 3.734 ± 0.048
3.956AspGlu: 3.956 ± 0.038
1.741AspPhe: 1.741 ± 0.028
6.418AspGly: 6.418 ± 0.056
1.427AspHis: 1.427 ± 0.025
1.992AspIle: 1.992 ± 0.027
1.299AspLys: 1.299 ± 0.027
6.261AspLeu: 6.261 ± 0.05
0.856AspMet: 0.856 ± 0.019
1.043AspAsn: 1.043 ± 0.021
4.424AspPro: 4.424 ± 0.047
1.57AspGln: 1.57 ± 0.025
4.777AspArg: 4.777 ± 0.047
2.696AspSer: 2.696 ± 0.033
3.357AspThr: 3.357 ± 0.04
4.882AspVal: 4.882 ± 0.042
1.082AspTrp: 1.082 ± 0.023
1.148AspTyr: 1.148 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
7.385GluAla: 7.385 ± 0.074
0.365GluCys: 0.365 ± 0.012
2.957GluAsp: 2.957 ± 0.032
3.591GluGlu: 3.591 ± 0.045
1.542GluPhe: 1.542 ± 0.024
4.425GluGly: 4.425 ± 0.041
1.526GluHis: 1.526 ± 0.021
2.274GluIle: 2.274 ± 0.031
1.525GluLys: 1.525 ± 0.025
6.834GluLeu: 6.834 ± 0.061
0.893GluMet: 0.893 ± 0.019
1.085GluAsn: 1.085 ± 0.021
3.386GluPro: 3.386 ± 0.039
2.371GluGln: 2.371 ± 0.032
5.215GluArg: 5.215 ± 0.054
2.653GluSer: 2.653 ± 0.029
2.932GluThr: 2.932 ± 0.033
4.542GluVal: 4.542 ± 0.043
0.824GluTrp: 0.824 ± 0.017
1.184GluTyr: 1.184 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.636PheAla: 3.636 ± 0.036
0.273PheCys: 0.273 ± 0.011
1.976PheAsp: 1.976 ± 0.028
1.497PheGlu: 1.497 ± 0.023
0.897PhePhe: 0.897 ± 0.022
3.019PheGly: 3.019 ± 0.039
0.631PheHis: 0.631 ± 0.015
0.694PheIle: 0.694 ± 0.017
0.562PheLys: 0.562 ± 0.014
2.694PheLeu: 2.694 ± 0.04
0.421PheMet: 0.421 ± 0.012
0.603PheAsn: 0.603 ± 0.015
1.432PhePro: 1.432 ± 0.023
0.728PheGln: 0.728 ± 0.017
1.869PheArg: 1.869 ± 0.029
1.488PheSer: 1.488 ± 0.023
2.081PheThr: 2.081 ± 0.029
2.367PheVal: 2.367 ± 0.031
0.463PheTrp: 0.463 ± 0.014
0.612PheTyr: 0.612 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
10.758GlyAla: 10.758 ± 0.077
0.863GlyCys: 0.863 ± 0.018
5.182GlyAsp: 5.182 ± 0.045
5.124GlyGlu: 5.124 ± 0.042
2.93GlyPhe: 2.93 ± 0.035
8.735GlyGly: 8.735 ± 0.085
2.291GlyHis: 2.291 ± 0.033
3.584GlyIle: 3.584 ± 0.036
2.529GlyLys: 2.529 ± 0.038
9.459GlyLeu: 9.459 ± 0.064
1.987GlyMet: 1.987 ± 0.03
1.868GlyAsn: 1.868 ± 0.028
4.897GlyPro: 4.897 ± 0.047
2.7GlyGln: 2.7 ± 0.039
7.547GlyArg: 7.547 ± 0.047
5.473GlySer: 5.473 ± 0.051
6.468GlyThr: 6.468 ± 0.056
7.577GlyVal: 7.577 ± 0.06
1.73GlyTrp: 1.73 ± 0.028
2.324GlyTyr: 2.324 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.642HisAla: 2.642 ± 0.036
0.219HisCys: 0.219 ± 0.009
1.37HisAsp: 1.37 ± 0.019
1.262HisGlu: 1.262 ± 0.023
0.652HisPhe: 0.652 ± 0.017
2.452HisGly: 2.452 ± 0.033
0.695HisHis: 0.695 ± 0.018
0.693HisIle: 0.693 ± 0.019
0.377HisLys: 0.377 ± 0.011
2.372HisLeu: 2.372 ± 0.03
0.336HisMet: 0.336 ± 0.011
0.403HisAsn: 0.403 ± 0.012
1.755HisPro: 1.755 ± 0.029
0.627HisGln: 0.627 ± 0.014
2.001HisArg: 2.001 ± 0.024
1.025HisSer: 1.025 ± 0.02
1.376HisThr: 1.376 ± 0.024
1.735HisVal: 1.735 ± 0.024
0.386HisTrp: 0.386 ± 0.013
0.504HisTyr: 0.504 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.766IleAla: 4.766 ± 0.046
0.292IleCys: 0.292 ± 0.01
2.285IleAsp: 2.285 ± 0.027
1.972IleGlu: 1.972 ± 0.028
0.724IlePhe: 0.724 ± 0.018
3.581IleGly: 3.581 ± 0.043
0.675IleHis: 0.675 ± 0.016
0.875IleIle: 0.875 ± 0.021
0.759IleLys: 0.759 ± 0.018
2.52IleLeu: 2.52 ± 0.034
0.469IleMet: 0.469 ± 0.012
0.751IleAsn: 0.751 ± 0.018
1.867IlePro: 1.867 ± 0.028
0.771IleGln: 0.771 ± 0.019
2.296IleArg: 2.296 ± 0.031
1.702IleSer: 1.702 ± 0.025
2.292IleThr: 2.292 ± 0.03
2.799IleVal: 2.799 ± 0.038
0.389IleTrp: 0.389 ± 0.012
0.555IleTyr: 0.555 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
3.09LysAla: 3.09 ± 0.039
0.119LysCys: 0.119 ± 0.007
1.425LysAsp: 1.425 ± 0.025
1.273LysGlu: 1.273 ± 0.025
0.48LysPhe: 0.48 ± 0.014
1.948LysGly: 1.948 ± 0.032
0.431LysHis: 0.431 ± 0.014
0.881LysIle: 0.881 ± 0.019
0.899LysLys: 0.899 ± 0.027
2.131LysLeu: 2.131 ± 0.028
0.407LysMet: 0.407 ± 0.013
0.57LysAsn: 0.57 ± 0.017
1.422LysPro: 1.422 ± 0.026
0.741LysGln: 0.741 ± 0.019
1.423LysArg: 1.423 ± 0.026
1.269LysSer: 1.269 ± 0.024
1.374LysThr: 1.374 ± 0.026
2.051LysVal: 2.051 ± 0.028
0.297LysTrp: 0.297 ± 0.011
0.527LysTyr: 0.527 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
14.769LeuAla: 14.769 ± 0.095
0.867LeuCys: 0.867 ± 0.018
6.753LeuAsp: 6.753 ± 0.059
4.71LeuGlu: 4.71 ± 0.05
2.659LeuPhe: 2.659 ± 0.039
9.37LeuGly: 9.37 ± 0.073
2.318LeuHis: 2.318 ± 0.031
3.249LeuIle: 3.249 ± 0.038
2.225LeuLys: 2.225 ± 0.031
11.234LeuLeu: 11.234 ± 0.098
1.652LeuMet: 1.652 ± 0.026
1.773LeuAsn: 1.773 ± 0.028
6.385LeuPro: 6.385 ± 0.052
2.275LeuGln: 2.275 ± 0.032
8.351LeuArg: 8.351 ± 0.069
5.272LeuSer: 5.272 ± 0.047
6.966LeuThr: 6.966 ± 0.049
8.907LeuVal: 8.907 ± 0.063
1.292LeuTrp: 1.292 ± 0.022
1.927LeuTyr: 1.927 ± 0.027
0.0LeuXaa: 0.0 ± 0.0
Met
2.248MetAla: 2.248 ± 0.032
0.149MetCys: 0.149 ± 0.008
0.897MetAsp: 0.897 ± 0.021
0.771MetGlu: 0.771 ± 0.017
0.478MetPhe: 0.478 ± 0.012
1.354MetGly: 1.354 ± 0.025
0.396MetHis: 0.396 ± 0.012
0.662MetIle: 0.662 ± 0.017
0.413MetLys: 0.413 ± 0.013
1.725MetLeu: 1.725 ± 0.024
0.296MetMet: 0.296 ± 0.011
0.418MetAsn: 0.418 ± 0.012
1.136MetPro: 1.136 ± 0.022
0.443MetGln: 0.443 ± 0.014
1.45MetArg: 1.45 ± 0.023
1.252MetSer: 1.252 ± 0.024
1.513MetThr: 1.513 ± 0.024
1.333MetVal: 1.333 ± 0.025
0.227MetTrp: 0.227 ± 0.009
0.345MetTyr: 0.345 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.261AsnAla: 2.261 ± 0.027
0.174AsnCys: 0.174 ± 0.007
1.004AsnAsp: 1.004 ± 0.022
0.853AsnGlu: 0.853 ± 0.017
0.516AsnPhe: 0.516 ± 0.015
2.0AsnGly: 2.0 ± 0.032
0.419AsnHis: 0.419 ± 0.013
0.714AsnIle: 0.714 ± 0.017
0.445AsnLys: 0.445 ± 0.014
1.737AsnLeu: 1.737 ± 0.029
0.314AsnMet: 0.314 ± 0.012
0.493AsnAsn: 0.493 ± 0.016
1.395AsnPro: 1.395 ± 0.026
0.547AsnGln: 0.547 ± 0.015
1.283AsnArg: 1.283 ± 0.023
1.056AsnSer: 1.056 ± 0.022
1.238AsnThr: 1.238 ± 0.025
1.465AsnVal: 1.465 ± 0.024
0.319AsnTrp: 0.319 ± 0.012
0.442AsnTyr: 0.442 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
7.939ProAla: 7.939 ± 0.067
0.332ProCys: 0.332 ± 0.012
4.479ProAsp: 4.479 ± 0.042
4.321ProGlu: 4.321 ± 0.046
1.558ProPhe: 1.558 ± 0.024
6.26ProGly: 6.26 ± 0.054
1.387ProHis: 1.387 ± 0.026
1.319ProIle: 1.319 ± 0.027
1.237ProLys: 1.237 ± 0.025
5.294ProLeu: 5.294 ± 0.042
0.974ProMet: 0.974 ± 0.02
0.945ProAsn: 0.945 ± 0.022
3.338ProPro: 3.338 ± 0.052
1.772ProGln: 1.772 ± 0.034
3.712ProArg: 3.712 ± 0.045
3.258ProSer: 3.258 ± 0.04
3.296ProThr: 3.296 ± 0.042
5.553ProVal: 5.553 ± 0.047
0.928ProTrp: 0.928 ± 0.021
1.465ProTyr: 1.465 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.757GlnAla: 3.757 ± 0.04
0.168GlnCys: 0.168 ± 0.009
1.484GlnAsp: 1.484 ± 0.026
1.512GlnGlu: 1.512 ± 0.023
0.697GlnPhe: 0.697 ± 0.017
2.382GlnGly: 2.382 ± 0.034
0.637GlnHis: 0.637 ± 0.015
1.11GlnIle: 1.11 ± 0.019
0.669GlnLys: 0.669 ± 0.017
3.095GlnLeu: 3.095 ± 0.041
0.514GlnMet: 0.514 ± 0.014
0.525GlnAsn: 0.525 ± 0.015
1.726GlnPro: 1.726 ± 0.028
1.249GlnGln: 1.249 ± 0.032
2.31GlnArg: 2.31 ± 0.033
1.312GlnSer: 1.312 ± 0.021
1.393GlnThr: 1.393 ± 0.024
2.454GlnVal: 2.454 ± 0.03
0.513GlnTrp: 0.513 ± 0.014
0.643GlnTyr: 0.643 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
9.328ArgAla: 9.328 ± 0.075
0.578ArgCys: 0.578 ± 0.013
4.123ArgAsp: 4.123 ± 0.045
4.722ArgGlu: 4.722 ± 0.049
2.327ArgPhe: 2.327 ± 0.027
5.521ArgGly: 5.521 ± 0.05
2.103ArgHis: 2.103 ± 0.033
3.293ArgIle: 3.293 ± 0.032
1.637ArgLys: 1.637 ± 0.025
8.631ArgLeu: 8.631 ± 0.067
1.703ArgMet: 1.703 ± 0.023
1.355ArgAsn: 1.355 ± 0.021
4.729ArgPro: 4.729 ± 0.048
2.313ArgGln: 2.313 ± 0.035
7.378ArgArg: 7.378 ± 0.066
3.972ArgSer: 3.972 ± 0.042
5.248ArgThr: 5.248 ± 0.048
5.762ArgVal: 5.762 ± 0.051
1.37ArgTrp: 1.37 ± 0.024
1.8ArgTyr: 1.8 ± 0.026
0.0ArgXaa: 0.0 ± 0.0
Ser
6.775SerAla: 6.775 ± 0.053
0.409SerCys: 0.409 ± 0.012
2.825SerAsp: 2.825 ± 0.031
2.481SerGlu: 2.481 ± 0.034
1.567SerPhe: 1.567 ± 0.027
6.086SerGly: 6.086 ± 0.054
0.98SerHis: 0.98 ± 0.021
1.482SerIle: 1.482 ± 0.025
1.127SerLys: 1.127 ± 0.017
4.9SerLeu: 4.9 ± 0.048
1.105SerMet: 1.105 ± 0.022
0.948SerAsn: 0.948 ± 0.019
3.206SerPro: 3.206 ± 0.041
1.281SerGln: 1.281 ± 0.02
3.635SerArg: 3.635 ± 0.035
2.959SerSer: 2.959 ± 0.041
3.176SerThr: 3.176 ± 0.037
4.475SerVal: 4.475 ± 0.04
0.934SerTrp: 0.934 ± 0.019
1.336SerTyr: 1.336 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
8.841ThrAla: 8.841 ± 0.073
0.441ThrCys: 0.441 ± 0.014
3.83ThrAsp: 3.83 ± 0.046
3.365ThrGlu: 3.365 ± 0.031
1.704ThrPhe: 1.704 ± 0.029
6.775ThrGly: 6.775 ± 0.061
1.21ThrHis: 1.21 ± 0.02
1.784ThrIle: 1.784 ± 0.029
1.3ThrLys: 1.3 ± 0.027
5.835ThrLeu: 5.835 ± 0.045
0.975ThrMet: 0.975 ± 0.022
1.082ThrAsn: 1.082 ± 0.024
4.074ThrPro: 4.074 ± 0.045
1.412ThrGln: 1.412 ± 0.023
3.871ThrArg: 3.871 ± 0.04
3.33ThrSer: 3.33 ± 0.041
4.003ThrThr: 4.003 ± 0.045
6.195ThrVal: 6.195 ± 0.044
0.969ThrTrp: 0.969 ± 0.022
1.464ThrTyr: 1.464 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
10.653ValAla: 10.653 ± 0.078
0.757ValCys: 0.757 ± 0.017
5.035ValAsp: 5.035 ± 0.041
4.818ValGlu: 4.818 ± 0.047
2.509ValPhe: 2.509 ± 0.035
6.698ValGly: 6.698 ± 0.058
1.956ValHis: 1.956 ± 0.032
2.907ValIle: 2.907 ± 0.035
1.829ValLys: 1.829 ± 0.032
9.454ValLeu: 9.454 ± 0.073
1.455ValMet: 1.455 ± 0.024
1.796ValAsn: 1.796 ± 0.027
5.201ValPro: 5.201 ± 0.047
2.182ValGln: 2.182 ± 0.027
7.234ValArg: 7.234 ± 0.058
4.482ValSer: 4.482 ± 0.038
5.839ValThr: 5.839 ± 0.053
8.206ValVal: 8.206 ± 0.072
1.201ValTrp: 1.201 ± 0.026
1.654ValTyr: 1.654 ± 0.024
0.0ValXaa: 0.0 ± 0.0
Trp
1.683TrpAla: 1.683 ± 0.024
0.15TrpCys: 0.15 ± 0.007
0.845TrpAsp: 0.845 ± 0.02
0.784TrpGlu: 0.784 ± 0.018
0.53TrpPhe: 0.53 ± 0.016
1.091TrpGly: 1.091 ± 0.021
0.406TrpHis: 0.406 ± 0.012
0.596TrpIle: 0.596 ± 0.015
0.411TrpLys: 0.411 ± 0.014
1.828TrpLeu: 1.828 ± 0.032
0.305TrpMet: 0.305 ± 0.01
0.45TrpAsn: 0.45 ± 0.014
0.775TrpPro: 0.775 ± 0.016
0.667TrpGln: 0.667 ± 0.018
1.331TrpArg: 1.331 ± 0.024
0.972TrpSer: 0.972 ± 0.021
1.102TrpThr: 1.102 ± 0.021
0.978TrpVal: 0.978 ± 0.018
0.356TrpTrp: 0.356 ± 0.011
0.4TrpTyr: 0.4 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.832TyrAla: 2.832 ± 0.032
0.178TyrCys: 0.178 ± 0.008
1.654TyrAsp: 1.654 ± 0.025
1.359TyrGlu: 1.359 ± 0.023
0.683TyrPhe: 0.683 ± 0.018
2.418TyrGly: 2.418 ± 0.031
0.403TyrHis: 0.403 ± 0.012
0.515TyrIle: 0.515 ± 0.015
0.449TyrLys: 0.449 ± 0.015
2.133TyrLeu: 2.133 ± 0.029
0.278TyrMet: 0.278 ± 0.01
0.448TyrAsn: 0.448 ± 0.016
1.069TyrPro: 1.069 ± 0.022
0.619TyrGln: 0.619 ± 0.016
1.805TyrArg: 1.805 ± 0.026
1.021TyrSer: 1.021 ± 0.022
1.268TyrThr: 1.268 ± 0.025
1.799TyrVal: 1.799 ± 0.028
0.381TyrTrp: 0.381 ± 0.012
0.506TyrTyr: 0.506 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8476 proteins (2742154 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski