Crimson clover cryptic virus 2
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M9VTV7|M9VTV7_9VIRU RNA-dependent RNA polymerase OS=Crimson clover cryptic virus 2 OX=1323528 PE=4 SV=1
MM1 pKa = 6.65 TTNSSNARR9 pKa = 11.84 LNKK12 pKa = 10.01 LKK14 pKa = 10.96 NNTVPLPFNATEE26 pKa = 4.25 VPEE29 pKa = 4.37 PATDD33 pKa = 4.05 LLTSMKK39 pKa = 9.92 EE40 pKa = 3.71 NTSAHH45 pKa = 6.22 SATANQWTIDD55 pKa = 4.26 LFPNMIPVLIHH66 pKa = 5.69 VMSAASHH73 pKa = 6.21 HH74 pKa = 6.58 ANAINFRR81 pKa = 11.84 EE82 pKa = 3.99 HH83 pKa = 6.8 SKK85 pKa = 9.98 TSAATVAMYY94 pKa = 10.8 HH95 pKa = 5.44 MAVVYY100 pKa = 9.69 GYY102 pKa = 11.15 FLLNDD107 pKa = 3.75 LNIRR111 pKa = 11.84 LNPSAHH117 pKa = 5.41 ARR119 pKa = 11.84 SWAEE123 pKa = 3.72 ASWKK127 pKa = 10.63 SEE129 pKa = 3.62 FVEE132 pKa = 4.31 FLYY135 pKa = 10.85 GLPVPEE141 pKa = 4.81 FLAPILSEE149 pKa = 4.01 YY150 pKa = 9.56 QHH152 pKa = 6.52 FVTDD156 pKa = 3.54 KK157 pKa = 9.32 TPNVHH162 pKa = 6.1 FTPSAAGFDD171 pKa = 3.42 HH172 pKa = 7.29 DD173 pKa = 4.07 QFFGRR178 pKa = 11.84 VFPLNMFAALHH189 pKa = 6.88 DD190 pKa = 4.23 STATLPSSSTYY201 pKa = 10.92 AQVLQDD207 pKa = 3.98 LFSRR211 pKa = 11.84 PLYY214 pKa = 10.18 TITDD218 pKa = 3.97 PAFTCYY224 pKa = 10.54 LPDD227 pKa = 3.95 LLGITLTAGEE237 pKa = 4.52 NVTTLNYY244 pKa = 9.87 INSKK248 pKa = 9.92 LYY250 pKa = 10.61 QVFTTIFNPVLFRR263 pKa = 11.84 DD264 pKa = 3.71 HH265 pKa = 5.86 QRR267 pKa = 11.84 RR268 pKa = 11.84 SSLAALSFKK277 pKa = 11.0 SPTYY281 pKa = 10.11 PNPNINAYY289 pKa = 10.03 DD290 pKa = 4.04 LLFSASVANLRR301 pKa = 11.84 EE302 pKa = 4.15 IKK304 pKa = 10.2 IVLQSIYY311 pKa = 10.83 KK312 pKa = 10.44 LFDD315 pKa = 3.01 GRR317 pKa = 11.84 VACKK321 pKa = 8.81 HH322 pKa = 5.5 TLSQFICEE330 pKa = 4.3 STSPSITKK338 pKa = 9.82 HH339 pKa = 5.7 GYY341 pKa = 7.84 STFPLPTWSHH351 pKa = 5.78 TEE353 pKa = 3.61 SDD355 pKa = 3.07 AKK357 pKa = 10.62 AIRR360 pKa = 11.84 FSGVTALVHH369 pKa = 6.22 VSEE372 pKa = 4.87 EE373 pKa = 3.76 DD374 pKa = 3.33 RR375 pKa = 11.84 AQDD378 pKa = 3.19 FCFLQRR384 pKa = 11.84 PAEE387 pKa = 4.67 AIPHH391 pKa = 5.21 VNEE394 pKa = 4.16 VPDD397 pKa = 3.26 IRR399 pKa = 11.84 YY400 pKa = 7.49 ATTEE404 pKa = 4.02 DD405 pKa = 4.35 PEE407 pKa = 4.63 TAVPLPANHH416 pKa = 6.28 ILVRR420 pKa = 11.84 RR421 pKa = 11.84 FPFAIRR427 pKa = 11.84 LRR429 pKa = 11.84 QEE431 pKa = 3.8 AANGFPRR438 pKa = 11.84 HH439 pKa = 6.74 DD440 pKa = 4.16 NDD442 pKa = 5.43 DD443 pKa = 3.54 LTKK446 pKa = 10.73 FSDD449 pKa = 3.99 HH450 pKa = 5.33 VHH452 pKa = 5.51 TAPRR456 pKa = 11.84 VLILDD461 pKa = 3.7 TTGDD465 pKa = 4.26 GIISAHH471 pKa = 5.21 TTTASGKK478 pKa = 9.58 IIEE481 pKa = 4.39 SFEE484 pKa = 4.67 LDD486 pKa = 3.04 GSTIEE491 pKa = 4.75 MPNADD496 pKa = 4.58 KK497 pKa = 11.17 SLGMQNCLFADD508 pKa = 3.42 SAIAYY513 pKa = 8.89 KK514 pKa = 9.6 YY515 pKa = 9.43 VRR517 pKa = 11.84 PGSFYY522 pKa = 10.78 RR523 pKa = 11.84 PRR525 pKa = 11.84 TQGSVLPPLNRR536 pKa = 11.84 APPNSRR542 pKa = 11.84 PRR544 pKa = 11.84 LPASSMLHH552 pKa = 6.68 DD553 pKa = 3.68 RR554 pKa = 11.84 TKK556 pKa = 11.13 VFLPQLNTRR565 pKa = 11.84 INEE568 pKa = 4.06 AVDD571 pKa = 3.04 IRR573 pKa = 11.84 TLPGLTMLNNVNCINYY589 pKa = 8.46 AQSFIGFKK597 pKa = 10.19 TVDD600 pKa = 2.95 ATRR603 pKa = 11.84 NDD605 pKa = 3.96 DD606 pKa = 3.81 VLDD609 pKa = 4.29 AVPGMPLNNLILWSPYY625 pKa = 9.86 TYY627 pKa = 9.79 TAYY630 pKa = 10.19 EE631 pKa = 4.44 DD632 pKa = 3.49 EE633 pKa = 4.85 TYY635 pKa = 10.56 PDD637 pKa = 3.83 PVFSNSRR644 pKa = 11.84 HH645 pKa = 5.29 YY646 pKa = 11.33 YY647 pKa = 7.96 LTNLRR652 pKa = 11.84 TIFGTDD658 pKa = 2.85 TNLIKK663 pKa = 10.53 AVHH666 pKa = 6.82 PYY668 pKa = 9.41 EE669 pKa = 4.91 AYY671 pKa = 9.61 PVSS674 pKa = 3.51
Molecular weight: 75.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.87
IPC2_protein 5.792
IPC_protein 5.893
Toseland 6.415
ProMoST 6.275
Dawson 6.211
Bjellqvist 6.198
Wikipedia 6.237
Rodwell 6.198
Grimsley 6.62
Solomon 6.211
Lehninger 6.211
Nozaki 6.491
DTASelect 6.693
Thurlkill 6.707
EMBOSS 6.678
Sillero 6.59
Patrickios 3.884
IPC_peptide 6.224
IPC2_peptide 6.59
IPC2.peptide.svr19 6.498
Protein with the highest isoelectric point:
>tr|M9VTV7|M9VTV7_9VIRU RNA-dependent RNA polymerase OS=Crimson clover cryptic virus 2 OX=1323528 PE=4 SV=1
MM1 pKa = 7.81 PFNAVRR7 pKa = 11.84 NYY9 pKa = 10.52 LNEE12 pKa = 3.53 RR13 pKa = 11.84 MTRR16 pKa = 11.84 LKK18 pKa = 10.63 QEE20 pKa = 3.51 WKK22 pKa = 8.49 TYY24 pKa = 10.3 QSTDD28 pKa = 2.96 HH29 pKa = 7.31 DD30 pKa = 5.06 PIKK33 pKa = 10.71 ILDD36 pKa = 4.3 TIQDD40 pKa = 3.59 PDD42 pKa = 3.32 YY43 pKa = 11.23 RR44 pKa = 11.84 RR45 pKa = 11.84 YY46 pKa = 10.67 LDD48 pKa = 3.33 NARR51 pKa = 11.84 FNSDD55 pKa = 3.17 NEE57 pKa = 4.48 MKK59 pKa = 10.57 HH60 pKa = 6.7 LMLNKK65 pKa = 9.91 EE66 pKa = 3.89 YY67 pKa = 9.61 STLVEE72 pKa = 5.36 AYY74 pKa = 8.28 RR75 pKa = 11.84 TDD77 pKa = 3.31 NAKK80 pKa = 9.94 KK81 pKa = 7.82 HH82 pKa = 4.88 QVYY85 pKa = 9.84 EE86 pKa = 3.83 LHH88 pKa = 6.59 QPIPNDD94 pKa = 3.58 AAPVIQSRR102 pKa = 11.84 LPAKK106 pKa = 10.1 GIKK109 pKa = 9.39 LVPLMYY115 pKa = 10.13 HH116 pKa = 6.17 YY117 pKa = 11.45 GHH119 pKa = 6.32 VTHH122 pKa = 7.42 DD123 pKa = 3.91 PVSLTDD129 pKa = 4.02 ANRR132 pKa = 11.84 SDD134 pKa = 4.71 DD135 pKa = 4.44 FEE137 pKa = 4.61 TQVVPDD143 pKa = 3.73 SDD145 pKa = 3.55 APTRR149 pKa = 11.84 FGYY152 pKa = 10.0 PIDD155 pKa = 3.56 VRR157 pKa = 11.84 IYY159 pKa = 10.33 NLICYY164 pKa = 9.56 RR165 pKa = 11.84 YY166 pKa = 8.73 SKK168 pKa = 10.59 YY169 pKa = 11.01 LEE171 pKa = 4.51 VINAYY176 pKa = 9.52 CRR178 pKa = 11.84 PIGTVNATFEE188 pKa = 4.58 DD189 pKa = 4.7 FNKK192 pKa = 10.12 EE193 pKa = 4.12 QIPSAPIDD201 pKa = 3.67 PKK203 pKa = 10.73 RR204 pKa = 11.84 KK205 pKa = 9.58 EE206 pKa = 3.91 NVLSHH211 pKa = 5.41 IHH213 pKa = 6.52 KK214 pKa = 10.53 FLDD217 pKa = 3.5 TKK219 pKa = 10.72 PYY221 pKa = 10.62 LPLHH225 pKa = 5.97 FVDD228 pKa = 4.11 TQFCKK233 pKa = 10.39 TPLVTGTGYY242 pKa = 10.81 HH243 pKa = 5.59 NRR245 pKa = 11.84 YY246 pKa = 9.47 SFKK249 pKa = 10.71 QKK251 pKa = 10.14 AHH253 pKa = 6.25 AKK255 pKa = 8.19 YY256 pKa = 10.18 SHH258 pKa = 6.48 PAEE261 pKa = 4.48 YY262 pKa = 8.59 ATKK265 pKa = 9.37 PISKK269 pKa = 10.03 GYY271 pKa = 9.88 FYY273 pKa = 11.23 NATYY277 pKa = 10.76 EE278 pKa = 3.93 NARR281 pKa = 11.84 TIIHH285 pKa = 6.83 FIKK288 pKa = 10.54 EE289 pKa = 3.72 YY290 pKa = 10.52 GLPFNVIRR298 pKa = 11.84 ADD300 pKa = 3.92 DD301 pKa = 3.98 KK302 pKa = 11.95 SEE304 pKa = 3.99 LTDD307 pKa = 3.83 SDD309 pKa = 3.74 VQKK312 pKa = 11.1 YY313 pKa = 10.08 INEE316 pKa = 4.19 ANSFFNDD323 pKa = 3.31 YY324 pKa = 7.74 PTLLFTRR331 pKa = 11.84 NHH333 pKa = 5.47 ISKK336 pKa = 10.27 RR337 pKa = 11.84 DD338 pKa = 3.55 GPLKK342 pKa = 10.16 VRR344 pKa = 11.84 PVYY347 pKa = 10.62 AVDD350 pKa = 4.65 DD351 pKa = 3.68 IFIIMEE357 pKa = 4.25 LMLTFPLTIQARR369 pKa = 11.84 KK370 pKa = 8.74 PSCCIMYY377 pKa = 10.42 GLEE380 pKa = 4.28 TIRR383 pKa = 11.84 GSNRR387 pKa = 11.84 YY388 pKa = 8.8 IEE390 pKa = 4.44 QIARR394 pKa = 11.84 DD395 pKa = 3.74 YY396 pKa = 11.09 STFFSLDD403 pKa = 2.74 WSSYY407 pKa = 6.99 DD408 pKa = 3.17 QRR410 pKa = 11.84 LPRR413 pKa = 11.84 VITDD417 pKa = 3.3 IYY419 pKa = 9.28 YY420 pKa = 9.62 TDD422 pKa = 4.27 FLRR425 pKa = 11.84 SLLVINNGYY434 pKa = 9.28 QPTYY438 pKa = 10.24 EE439 pKa = 4.22 YY440 pKa = 8.37 PTYY443 pKa = 10.44 PDD445 pKa = 3.72 LDD447 pKa = 3.61 EE448 pKa = 5.24 HH449 pKa = 6.91 KK450 pKa = 10.3 LYY452 pKa = 11.02 HH453 pKa = 7.43 RR454 pKa = 11.84 MDD456 pKa = 3.79 NLLHH460 pKa = 6.85 FLHH463 pKa = 6.72 LWYY466 pKa = 11.33 NNMTFLLPDD475 pKa = 4.16 GYY477 pKa = 11.15 AYY479 pKa = 10.7 RR480 pKa = 11.84 RR481 pKa = 11.84 TSCGVPSGLYY491 pKa = 7.56 NTQYY495 pKa = 11.31 LDD497 pKa = 3.35 SFGNLFLIIDD507 pKa = 4.03 AMLEE511 pKa = 3.96 FGFTDD516 pKa = 3.75 SEE518 pKa = 4.08 IQKK521 pKa = 10.67 FILLVLGDD529 pKa = 5.24 DD530 pKa = 3.93 NTGMTTIPICRR541 pKa = 11.84 MFNFITFLEE550 pKa = 4.95 KK551 pKa = 10.76 YY552 pKa = 10.2 ALEE555 pKa = 4.49 RR556 pKa = 11.84 YY557 pKa = 10.39 NMVLSTTKK565 pKa = 10.58 SVLTTFRR572 pKa = 11.84 SKK574 pKa = 10.49 IEE576 pKa = 3.95 SLGYY580 pKa = 9.35 QCNHH584 pKa = 6.66 GSPKK588 pKa = 9.96 RR589 pKa = 11.84 DD590 pKa = 2.8 IDD592 pKa = 3.69 KK593 pKa = 11.03 LVAQLCYY600 pKa = 9.98 PEE602 pKa = 5.79 NGLKK606 pKa = 9.45 PHH608 pKa = 6.12 TMAARR613 pKa = 11.84 AIGIAYY619 pKa = 9.85 ASAGQDD625 pKa = 3.3 DD626 pKa = 4.87 MFHH629 pKa = 6.69 SFCQDD634 pKa = 2.32 VYY636 pKa = 11.4 NIFRR640 pKa = 11.84 SDD642 pKa = 3.51 YY643 pKa = 11.0 RR644 pKa = 11.84 PDD646 pKa = 3.55 DD647 pKa = 3.83 RR648 pKa = 11.84 MNLHH652 pKa = 7.07 FKK654 pKa = 10.58 RR655 pKa = 11.84 QIFHH659 pKa = 6.44 NLEE662 pKa = 4.65 DD663 pKa = 4.34 GMPDD667 pKa = 3.93 LAPPIVPPFPSLYY680 pKa = 10.08 EE681 pKa = 3.62 IRR683 pKa = 11.84 EE684 pKa = 3.76 MYY686 pKa = 10.25 AYY688 pKa = 10.76 YY689 pKa = 10.45 KK690 pKa = 10.81 GPLDD694 pKa = 5.61 FAPKK698 pKa = 8.95 WNYY701 pKa = 9.41 AHH703 pKa = 6.86 FMSDD707 pKa = 3.44 PDD709 pKa = 3.77 TTPPYY714 pKa = 10.32 SKK716 pKa = 9.81 TMRR719 pKa = 11.84 DD720 pKa = 3.47 YY721 pKa = 10.87 EE722 pKa = 4.28 AEE724 pKa = 4.06 NNISSRR730 pKa = 11.84 VAPTFEE736 pKa = 4.21 TVVPSTINLPP746 pKa = 3.63
Molecular weight: 87.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.879
IPC2_protein 5.931
IPC_protein 6.033
Toseland 6.465
ProMoST 6.44
Dawson 6.351
Bjellqvist 6.351
Wikipedia 6.376
Rodwell 6.351
Grimsley 6.634
Solomon 6.351
Lehninger 6.351
Nozaki 6.634
DTASelect 6.825
Thurlkill 6.854
EMBOSS 6.825
Sillero 6.737
Patrickios 3.503
IPC_peptide 6.376
IPC2_peptide 6.678
IPC2.peptide.svr19 6.658
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1420
674
746
710.0
81.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.042 ± 1.352
1.127 ± 0.159
6.479 ± 0.766
4.225 ± 0.148
5.634 ± 0.103
3.31 ± 0.131
3.521 ± 0.127
5.915 ± 0.487
4.155 ± 0.8
8.944 ± 0.372
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.324 ± 0.366
6.127 ± 0.27
7.254 ± 0.211
2.676 ± 0.203
5.282 ± 0.26
6.761 ± 0.843
7.676 ± 0.726
5.0 ± 0.53
0.634 ± 0.073
5.915 ± 1.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here