Sediminibacterium goheungense
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3224 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R6ITU4|A0A4R6ITU4_9BACT Sortilin (Neurotensin receptor 3) OS=Sediminibacterium goheungense OX=1086393 GN=BC659_2650 PE=4 SV=1
MM1 pKa = 7.6 NKK3 pKa = 8.21 FTRR6 pKa = 11.84 LWVSLVSLTLFTVFSAFRR24 pKa = 11.84 FTGLPEE30 pKa = 4.3 LGGAEE35 pKa = 4.08 KK36 pKa = 10.03 MYY38 pKa = 10.72 NNPGKK43 pKa = 9.94 VVKK46 pKa = 10.43 DD47 pKa = 3.25 ATVVRR52 pKa = 11.84 NFTTRR57 pKa = 11.84 VLKK60 pKa = 10.9 NITRR64 pKa = 11.84 AAGFTGRR71 pKa = 11.84 TFNGNSNISLTVPGNMSFSNTPGTCGRR98 pKa = 11.84 VVTYY102 pKa = 9.74 AAPTAQANAVSVEE115 pKa = 3.63 FDD117 pKa = 3.31 YY118 pKa = 11.3 TGNIQTFTVPAGVTSITIKK137 pKa = 11.03 GIGADD142 pKa = 3.79 GGNSLSSGVNGGRR155 pKa = 11.84 GASLTGTFAVTPGQTIYY172 pKa = 10.46 MVVGQRR178 pKa = 11.84 GSDD181 pKa = 3.5 DD182 pKa = 3.65 FADD185 pKa = 4.71 FGLGAAGGGGGTYY198 pKa = 10.06 ISATPFGTPSAVPLMVAGAGGGAAAIVDD226 pKa = 4.07 NNIHH230 pKa = 6.57 ANANSVDD237 pKa = 3.5 GNYY240 pKa = 10.73 GVTGDD245 pKa = 4.55 GGDD248 pKa = 3.66 GALGTGGSGGEE259 pKa = 3.93 AGTNSGAGAGWLADD273 pKa = 3.64 GLSDD277 pKa = 4.92 INSGDD282 pKa = 3.45 LTGGLSQFGTNPFAGGSLFGVLYY305 pKa = 10.53 GGYY308 pKa = 10.23 GGGGSAGFVGGGGGGGYY325 pKa = 9.95 SGGGGGGDD333 pKa = 3.14 NGGGGGGGSFNGGTNPVNVPGVDD356 pKa = 3.59 GDD358 pKa = 4.63 DD359 pKa = 3.71 GAGGNGKK366 pKa = 9.04 VVITYY371 pKa = 7.42 GTSVTVTQIAGLPSGATFPVGVTTNTFQATDD402 pKa = 3.74 GVNTTTASFTVTVNDD417 pKa = 3.52 TEE419 pKa = 5.0 APVLTAPSNITYY431 pKa = 7.79 TIPAGSSNCNAAAVNLGTPTVSDD454 pKa = 3.27 NCGANTPTNNAPSLFPLGTTTVTWSVTDD482 pKa = 2.82 IHH484 pKa = 6.99 GNPATATQQVTVVAPEE500 pKa = 3.64 IRR502 pKa = 11.84 IASASFVFSILNGSTSIDD520 pKa = 3.73 LATGTDD526 pKa = 3.98 FGTTNINNQVTRR538 pKa = 11.84 TYY540 pKa = 10.46 IIDD543 pKa = 3.81 NAGGTADD550 pKa = 4.83 LSVNSINIAGANPSFFTVGGITLPATIPAGQTTTFTVTYY589 pKa = 8.94 SASAGGTHH597 pKa = 6.44 NAIVQLNNNDD607 pKa = 3.93 CDD609 pKa = 3.39 EE610 pKa = 4.39 SAYY613 pKa = 11.1 SFAIQGVTNQATTPITGNTSVCVSGTTQLANVTAGGTWSSANAAVATVDD662 pKa = 3.68 ANGLVTAVSAGTANISYY679 pKa = 8.18 TMPGGTVQVTVTVNAAPTAAISANGPTTFCAGGSVTLTATGGASYY724 pKa = 10.35 LWSNGSTTSSITVSAAGTYY743 pKa = 10.41 SVVATSAEE751 pKa = 4.4 GCSSASVSSSVTVNPVPTAAISADD775 pKa = 3.52 GPTTFCAGGSVTLTATGGASYY796 pKa = 10.28 LWSNGATTSSITVSSGGVYY815 pKa = 10.34 SVVATSAEE823 pKa = 4.4 GCSSASVSSSVTVNPVPTAAISAA846 pKa = 4.02
Molecular weight: 81.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.971
IPC2_protein 4.075
IPC_protein 4.088
Toseland 3.859
ProMoST 4.266
Dawson 4.088
Bjellqvist 4.24
Wikipedia 4.05
Rodwell 3.91
Grimsley 3.77
Solomon 4.088
Lehninger 4.05
Nozaki 4.202
DTASelect 4.495
Thurlkill 3.923
EMBOSS 4.05
Sillero 4.215
Patrickios 0.388
IPC_peptide 4.075
IPC2_peptide 4.19
IPC2.peptide.svr19 4.079
Protein with the highest isoelectric point:
>tr|A0A4R6IWM2|A0A4R6IWM2_9BACT NIPSNAP protein OS=Sediminibacterium goheungense OX=1086393 GN=BC659_2423 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPHH8 pKa = 4.67 KK9 pKa = 9.7 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.4 SVHH16 pKa = 5.0 GFRR19 pKa = 11.84 KK20 pKa = 10.11 RR21 pKa = 11.84 MQTANGRR28 pKa = 11.84 KK29 pKa = 8.71 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.04 GRR39 pKa = 11.84 KK40 pKa = 8.7 KK41 pKa = 10.08 LTVSSEE47 pKa = 4.1 KK48 pKa = 10.7 GLKK51 pKa = 9.34
Molecular weight: 6.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.891
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.179
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3224
0
3224
1116394
29
4357
346.3
38.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.563 ± 0.044
0.878 ± 0.014
5.046 ± 0.037
5.617 ± 0.062
4.881 ± 0.033
6.877 ± 0.042
1.94 ± 0.025
7.288 ± 0.04
6.85 ± 0.052
9.342 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.503 ± 0.026
5.312 ± 0.054
3.85 ± 0.025
4.034 ± 0.029
4.112 ± 0.03
6.261 ± 0.05
6.024 ± 0.081
6.36 ± 0.032
1.272 ± 0.017
3.992 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here