Gordonia phage Spooky

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Langleyhallvirinae; unclassified Langleyhallvirinae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DFE9|A0A514DFE9_9CAUD RecA-like DNA recombinase OS=Gordonia phage Spooky OX=2591210 GN=63 PE=4 SV=1
MM1 pKa = 7.43TYY3 pKa = 8.71STRR6 pKa = 11.84HH7 pKa = 5.2AVSAGGFRR15 pKa = 11.84WRR17 pKa = 11.84GPDD20 pKa = 3.37YY21 pKa = 10.67PYY23 pKa = 11.09DD24 pKa = 3.88LPGPGDD30 pKa = 4.43MLPEE34 pKa = 4.95DD35 pKa = 4.21PDD37 pKa = 3.97EE38 pKa = 4.71PEE40 pKa = 3.82EE41 pKa = 4.24MYY43 pKa = 10.91PNPAYY48 pKa = 10.14GQQDD52 pKa = 3.61PWRR55 pKa = 11.84QPP57 pKa = 2.91

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DFG3|A0A514DFG3_9CAUD Uncharacterized protein OS=Gordonia phage Spooky OX=2591210 GN=86 PE=4 SV=1
MM1 pKa = 7.36TSKK4 pKa = 10.51PCNRR8 pKa = 11.84PDD10 pKa = 3.62CEE12 pKa = 3.97RR13 pKa = 11.84PATRR17 pKa = 11.84RR18 pKa = 11.84GLCHH22 pKa = 7.02ADD24 pKa = 3.53YY25 pKa = 8.6MAHH28 pKa = 6.83MNRR31 pKa = 11.84QMAYY35 pKa = 9.36GRR37 pKa = 11.84WEE39 pKa = 3.88SGYY42 pKa = 11.05VDD44 pKa = 3.55AQPARR49 pKa = 11.84EE50 pKa = 3.98HH51 pKa = 5.78VANLRR56 pKa = 11.84AAGMGTRR63 pKa = 11.84TIAQTAGISRR73 pKa = 11.84TILQCLIHH81 pKa = 6.39GKK83 pKa = 8.81PRR85 pKa = 11.84PGAEE89 pKa = 3.77RR90 pKa = 11.84RR91 pKa = 11.84DD92 pKa = 3.95PPSKK96 pKa = 9.89TIAKK100 pKa = 9.74HH101 pKa = 4.31NAEE104 pKa = 5.24KK105 pKa = 10.44ILAVPIPDD113 pKa = 3.88DD114 pKa = 3.73VRR116 pKa = 11.84DD117 pKa = 3.73GSGNIDD123 pKa = 3.26ALGTRR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84LQALIAYY137 pKa = 7.5GYY139 pKa = 7.64SQRR142 pKa = 11.84DD143 pKa = 3.18LSRR146 pKa = 11.84RR147 pKa = 11.84LGYY150 pKa = 8.28TADD153 pKa = 3.63NLTSIVNGSLAAVKK167 pKa = 9.91PSTRR171 pKa = 11.84EE172 pKa = 3.39RR173 pKa = 11.84VSRR176 pKa = 11.84LFSEE180 pKa = 4.72LQVTPGNCSRR190 pKa = 11.84SRR192 pKa = 11.84NRR194 pKa = 11.84GRR196 pKa = 11.84ANRR199 pKa = 11.84WPLPLDD205 pKa = 3.34WDD207 pKa = 4.24EE208 pKa = 4.9DD209 pKa = 4.53TIDD212 pKa = 5.03DD213 pKa = 4.08PTAEE217 pKa = 4.18ATRR220 pKa = 11.84SIIRR224 pKa = 11.84RR225 pKa = 11.84YY226 pKa = 10.32DD227 pKa = 3.08PRR229 pKa = 11.84QDD231 pKa = 3.22VEE233 pKa = 4.59KK234 pKa = 10.58IADD237 pKa = 3.58RR238 pKa = 11.84RR239 pKa = 11.84TRR241 pKa = 11.84VARR244 pKa = 11.84LLDD247 pKa = 4.23AGLSASEE254 pKa = 4.03IAVQLRR260 pKa = 11.84CTVRR264 pKa = 11.84TIEE267 pKa = 4.1RR268 pKa = 11.84DD269 pKa = 2.9KK270 pKa = 10.8TAIRR274 pKa = 11.84EE275 pKa = 4.04ADD277 pKa = 3.7SLSAASS283 pKa = 3.74

Molecular weight: 31.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

18085

28

1871

165.9

18.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.164 ± 0.463

1.399 ± 0.205

6.774 ± 0.272

5.861 ± 0.252

2.577 ± 0.157

8.217 ± 0.352

2.488 ± 0.194

4.567 ± 0.174

3.434 ± 0.291

7.492 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.124

2.986 ± 0.15

5.447 ± 0.195

3.561 ± 0.154

7.564 ± 0.356

5.657 ± 0.19

6.591 ± 0.293

7.382 ± 0.244

2.317 ± 0.105

2.217 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski